• Journal of Microbiology and Biotechnology
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• 제18권5호
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• pp.845-851
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• 2008

TolC is an outer membrane porin protein and an essential component of drug efflux and type-I secretion systems in Gram-negative bacteria. TolC comprises a periplasmic $\alpha$-helical barrel domain and a membrane-embedded $\beta$-barrel domain. TdeA, a functional and structural homolog of TolC, is required for toxin and drug export in the pathogenic oral bacterium Actinobacillus actinomycetemcomitans. Here, we report the expression of the periplasmic domain of TdeA as a soluble protein by substitution of the membrane-embedded domain with short linkers, which enabled us to purify the protein in the absence of detergent. We confirmed the structural integrity of the TdeA periplasmic domain by size-exclusion chromatography, circular dichroism spectroscopy, and electron microscopy, which together showed that the periplasmic domain of the TolC protein family fold correctly on its own. We further demonstrated that the periplasmic domain of TdeA interacts with peptidoglycans of the bacterial cell wall, which supports the idea that completely folded TolC family proteins traverse the peptidoglycan layer to interact with inner membrane transporters.

• 동력기계공학회지
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• 제10권4호
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• pp.17-24
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• 2006

The turbulent flow field characteristics of a gun-type gas burner with and without a duct were investigated under the isothermal condition of non-combustion. Vectors and mean velocities were measured by hot-wire anemometer system with an X-type hot-wire probe in this paper. The turbulent flow field with a duct seems to cause a counter-clockwise recirculation flow from downstream to upstream due to the unbalance of static pressure between a main jet flow and a duct wall. Moreover, the recirculation flow seems to expand the main jet flow to the radial and to shorten it to the axial. Therefore, the turbulent flow field with a duct increases a radial momentum but decreases a axial momentum. As a result, an axial mean velocity component with a duct above the downstream range of about X/R=1.5 forms a smaller magnitude than that without a duct in the inner part of a burner, but it shows the opposite trend in the outer part.

• 한국임상수의학회지
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• 제19권1호
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• pp.107-109
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• 2002

혼수상태를 보인 8개월령 타조가 증상 발현 이틀 후 폐사되었다. 부검시 육안상으로 두꺼워진 기낭벽에는 녹회색의 곰팡이가, 간에는 다발성의 작은 결절들이 관찰되었다. 현미경상으로 기낭막, 폐, 간의 육아종성 병소는 많은 격벽을 가지며 가지를 뻗는 곰팡이성 균사를 함유하고 있었다. 곰팡이의 전형적인 포자머리는 감염된 두꺼워진 기낭 내막에서 관찰되었다 이 증례는 Aspergillus fumigatus에 의해 만성의 전신적인 사상균성 기낭염과 폐렴을 보인 동물원 타조에 대한 보고이다

• 한국정밀공학회지
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• 제14권2호
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• pp.102-112
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• 1997

This paper describes the method that can construct kinematically admissible velocity fields for forging of gear-like components which have tooth shape around the cylinder. The kinematically admissible velo- city fields for the various gear-like components, involute spur gear, trapezoidal spline, square spline, ser- ration and trochoidal gear, were constructed by pilling up the velocity components according to the shape of tooth and billet. The billets, of hollow and solid, were Al 2218 and 2024. To verify the method, the analyses and experiments were carried out and compared with each other. For analyses, the half pitches of com- ponents were divided into several deformation regions based on their tooth profile. A neutral surface was used to represent the inner flow of material during forging. Its location varied with the energy optimazation and its contour varied with the number of teeth. In experiment, the contour of material filling up the tooth zone is hyperbolic curve caused by the frictional drag on the interface of die-wall/workpiece but, in the analysis, it is an arc which retains the same contour during all forging operation.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2011년도 춘계학술대회 논문집
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• pp.261-262
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• 2011

Conventional automatic transmission system includes a hydrodynamic torque converter to transfer engine torque from an engine crank shaft to a rotatable input members, which are of complex design permitting them to serve several functions. These are clutches or brakes which couple the rotatable input member to member of a planetary gear set. The annulus gear for an automatic transmission is a monolithic gear having a set of gear teeth formed on an inner surface which is coupling with a set of planetary gear. In this study, the flow forming method is applied to the manufacturing of the annulus gear. This cold forming is proper method in order to manufacture dimensionally precise and round hollow components such as annulus gear. By pre-calculated amount of wall thickness reduction, the seamless tube of SAE1026 is compressed above its yield strength, plastically deformed and made to flow in several roll passes. According to this study, the desired geometry of the annulus gear can be achieved when the outer diameter and the thickness of the tube are properly decreased by compressed roll passes and the available material volume is easily forced to flow longitudinally over the shape of mandrel.

• Nuclear Engineering and Technology
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• 제35권2호
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• pp.144-153
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• 2003

The object of this paper is the probabilistic failure analysis on the cladding performance of WPF(Whole Pin Furnace) test fuel pins under transient conditions, and analysis of the KALIMER fuel pin using the preceding analysis. The cumulative damage estimation and Weibull probability estimation of WPF test are performed. The probabilistic method was adapted for these analyses to determine the effective thickness thinning due to eutectic penetration depth. In the results, it is difficult to assume that a brittle layer depth made by eutectic reaction is all of the thickness reduction due to cladding thinning. About 93% cladding thinning of the eutectic penetration depth is favorable as an effective thickness of cladding. And the unreliability of the KALIMER driver fuel pin under the same WPF test condition is lower than that of the WPF pin because of the higher plenum-fuel volume ratio and lower cladding inner radius vs. thickness ratio. KALIMER fuel pin developed from conceptual design has a more stable transient performance for a failure mechanism due to fission gas buildup than the WPF pin.

• Nuclear Engineering and Technology
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• 제53권11호
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• pp.3625-3634
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• 2021

The crossflow is the key phenomenon in turbulent flow inside rod bundles. In order to establish confidence on application of computational fluid dynamics (CFD) to simulate the crossflow in rod bundles, three Reynolds-Averaged Navier Stokes (RANS) models i.e. the realizable k-ε model, the k-ω SST model and the Reynolds stress model (RSM), and the Large Eddy simulations (LES) with the Wall-Adapting Local Eddy-viscosity (WALE) model are validated based on the Particle Image Velocimetry (PIV) flow measurement experiment in a 5 × 5 rod bundle. In order to investigate effects of periodic boundary condition in the gap, the numerical results obtained with four inner subchannels are compared with that obtained with the whole 5 × 5 rod bundle. The results show that periodic boundaries in the gaps produce strong errors far downstream of the spacer grid, and therefore the full 5 × 5 rod bundle should be simulated. Furthermore, it can be concluded, that the realizable k-ε model can only provide reasonable results very close to the spacer grid, while the other investigated models are in good agreement with the experimental data in the whole downstream flow in the rod bundle. The LES approach shows superiority to the RANS models.

• International Journal of Naval Architecture and Ocean Engineering
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• 제11권1호
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• pp.572-583
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• 2019

This paper aims to assess the applicability of the Runge Kutta Discontinuous Galerkin-Direct Ghost Fluid Method to the internal explosion inside a water-filled tube, which previously was studied by many researchers in separate works. Once the explosive charge located at the inner center of the water-filled tube explodes, the tube wall is subjected to an extremely high intensity fluid loading and deformed. The deformation causes a modification of the field of fluid flow in the region near the water-structure interface so that has substantial influence on the response of the structure. To connect the structure and the fluid, valid data exchanges along the interface are essential. Classical fluid structure interaction simulations usually employ a matched meshing scheme which discretizes the fluid and structure domains using a single mesh density. The computational cost of fluid structure interaction simulations is usually governed by the structure because the size of time step may be determined by the density of structure mesh. The finer mesh density, the better solution, but more expensive computational cost. To reduce such computational cost, a non-matched meshing scheme which allows for different mesh densities is employed. The coupled numerical approach of this paper has fewer difficulties in the implementation and computation, compared to gas dynamics based approach which requires complicated analytical manipulations. It can also be applied to wider compressible, inviscid fluid flow analyses often found in underwater explosion events.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2017년도 학술발표회
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• pp.66-66
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• 2017

하천에 설치된 저낙차 보의 여수로나 위어는 일반적으로 도수가 발생하도록 설계한다. 이러한 수공구조물위를 통과하는 흐름이 갖는 강한 운동에너지를 소산시키는데 도수는 중요한 역할을 한다. 난류흐름과 도수의 수면부근에서 형성되는 롤러 형태의 와류가 이러한 에너지 소산의 대부분을 유발한다. 이 연구에서는 여수로에서 발생하는 수중 도수 현상을 고해상도 3차원 수치모의를 통해서 재현하고 실험실에서 PIV를 이용해서 관측한 실험결과와 비교분석 한다. 아울러 수치모의 결과는 수중의 바닥면을 따라 발생하는 ?의 내부와 외부 전단층 그리고 도수에 의해서 발생하는 제순한 와류의 동적 거동 특성을 제시한다.

• 한국기계가공학회지
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• 제19권11호
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• pp.35-41
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• 2020

The structure of the femur bone was analyzed. Moreover, the mechanical strength of the bone was determined by considering two parameters, namely, the outer wall thickness and inner filling density to realize the 3D printing of a cortical bone and spongy bone by using a fused deposition modeling type 3D printer and ABS material. A basic experiment was conducted to evaluate the variation trend in the mechanical strength of the test specimens with the change in the parameters. Based on the results, the parameters corresponding to the highest mechanical strength were selected and applied to the artificial bone, and the mechanical strength of the artificial bones was examined under a load. Moreover, we proposed an approximation method for the 3D printing parameters to enable the comparison of the actual bones and artificial bones in terms of the strength and weight.