• Korean Journal of Ichthyology
• /
• v.19 no.2
• /
• pp.112-119
• /
• 2007

A histological study on development and transformation of the oocyte' follicle cell for Korean four sillurid fishes, Liobagrus obesus, L. mediadiposalis, Pseudobagrus koreanus, and P. brevicorpus was performed by light and electron microscopes. The follicular layer surrounding the oocyte consisted of an outer theca cell and an inner follicle cell (granulosa cell). The follicle cells of the oocyte were flatten cells at early oocyte but during vitellogenesis they were transformed it to a single layer of cuboidal cell, then to a single columnar cell layer, and finally to a layer covered with a substance secreted by themselves. Although the development and transformation of the follicle cells was similar to four species, the secreted materials, called an adhesive membrane, were divided into two types in its appearance and nature. Firstly, a jelly coat-like type was found in L. obesus and L. mediadiposalis, which they are presumed to be polysaccharides and mucoproteins in its nature and secondly, a granular type in P. koreanus and P. brevicopus, being mucoprotein. A zona radiata with about $0.6{\sim}3.1{\mu}m$ thin was present below the adhesive material secreted by the transformed-follicle cell's activity. The zona radiata was composed of two layers, a thin externa and a thick interna.

• Korean Journal of Ecology and Environment
• /
• v.33 no.3 s.91
• /
• pp.197-205
• /
• 2000

Factors most strongly related to zooplankton growth rates were studied along a lake and a river stretch in the middle part of the lowland River Spree. The study was conducted at the lake inflow (S1), the lake outflow (S2), and at the end of a 21 km stretch of the outflow (S3) from March to November of 1999. Total zooplankton biomass increased significantly at S2 and then sharply decreased at S3. The abundance of microzooplankton (rotifers and nauplii) was strongly higher than macrozooplankton (cladocerans and copepodids) at all station. However, macrozooplankton biomass (${\mu}$g dw 1$^{-1}$) was similar or much higher than microzooplankton biomass. Large-bodied cladocerans (Daphnia cucullata) dominated at S2 while small-bodied cladocerans (Bosmina longirostris) dominated at S1 and S3. Patterns in growth rates (r$_{t}$ in d$^{-1}$ of the major zooplankton community were greatly different between S1 and S2 (lake stretch) and between S2 and S3 (river stretch). In the lake, growth rates generally were positive, while values of growth rates were negative in the river stretch. Among the environmental variables considered, partial retention time (PRT, d$^{-1}$) seemed to play the most important role in determining characteristics of the zooplankton community structure in the middle part of River Spree.

• Ocean and Polar Research
• /
• v.40 no.2
• /
• pp.69-75
• /
• 2018

We investigated the effects of environmental variations on the growth and survival rate of Stichopus japonicus to determine the optimum environmental conditions for its growth. Literature studies and a 12 month-long diver survey were carried out to understand the habitat, ecology and size of the surveyed area. Based on the collected data, we suggested optimum habitat conditions for releasing S. japonicus. Experiments on releasing S. japonicus were conducted in the breakwater of the Hwagye fishing cooperative in Hwagey-ri, Namhae-un, Gyeongsangnam-do, Korea. To implement the experiments, we divided the surveyed area into 4 sub-areas with different characteristics: (1) sand and silt zone; (2) artificial sea cucumber bank zone; (3) artificial rock bank zone; and (4) marine algae zone. The experiment lasted for 12 months. We released 32,000 sea cucumbers over $120m^2$ of each of the sand and silt zone, artificial rock bank zone and marine algae zone and released 6,000 sea cucumbers over $120m^2$ of the artificial sea cucumber bank zone. The average density of the released sea cucumbers from day 30 to day 360 after the releasing was conducted was the highest in the artificial sea cucumber bank zone ($23.7animal/m^2$), which was followed by artificial rock bank zone ($2.0animal/m^2$), marine algae zone ($1.9animal/m^2$) and sand and silt zone ($0.8animal/m^2$). The analysis on growth showed that the initial average weight of 2.3 g increased on day 360 after the releasing to 12.5 g in the artificial sea cucumber rank zone, 20.2 g in the sand and silt zone, 23.3 g in the artificial rock bank zone and 22.9 g in the marine algae zone. Results from the experiment along with the literature analysis suggest the following optimum habitat conditions: $10-15^{\circ}C$ water temperature; 28-34 psu salinity; 5-10 m water depth; 0.2-0.5 m/s velocity; rock, stone and muddy sand as substrate; and less than 20% mud in the substrate.

• Journal of Fisheries and Marine Sciences Education
• /
• v.24 no.6
• /
• pp.845-853
• /
• 2012

To investigate the role of temperatures and photoperiods as environmental cues regulating reproductive rhythm in Pseudobagrus fulvidraco, rearing experiments were conducted using sveral rearing regimes conbined with photoperiods and water temperatures during gonadal degeneration periods. Gonadosomatic index (GSI) in control was $8.16{\pm}1.50%$, while in other experiment GSI levels in female were lower than that in the control. In case of experimental precinct of 9 light (L) and 15 L, GSI levels were decreased. But GSI level with $20^{\circ}C$ was no diffrence after 40 and 60 days. GSI level in male of control was $0.35{\pm}0.05%$. GSI under 9 L and $25^{\circ}C$ was similar to that in control, whereas its level in other experiments was lower than that in control. Testosterone (T) of female was $3.68{\pm}0.22$ ng/mL at experimental precinct. In case of 9 L and 15 L, concentration of T were lower than experimental precinct in all of water temperature. Estradiol-$17{\beta}$ ($E_2$) and $7{\alpha}$, $20{\beta}$-dihydroxy-4-pregnen-3-one ($17{\alpha}20{\beta}OHP$) levels of female were $0.42{\pm}0.02$ and $0.83{\pm}0.01$ ng/mL at experimental precinct. $E_2$ levels of 9 and 15 L were higher than experimental precinct and $17{\alpha}20{\beta}OHP$ levels of 9 and 15 L were higher than experimental precinct. In case of T and 11-ketotestosterone levels were $0.69{\pm}0.11$ and $.62{\pm}0.03$ ng/mL in male. During the period of gonadal degeneration, gonadal maturation did not occur in any of the experimental regimes. However, comparatively high levels of $E_2$ observed at low temperature regimes ($20^{\circ}C$) regardless of photoperiods.

• Korean Journal of Ecology and Environment
• /
• v.54 no.4
• /
• pp.384-389
• /
• 2021

This study was conducted on October 23, 2020 at the Cheongpyeong Reservoir located in Seorakmyeon, Gapyeong-gun, Gyeonggi-do, and analyzed the horizontal and vertical distribution patterns of fish based on data obtained using fishfinder. The total surface area of fishfinder survey conducted was 782,853 m², and where the water depth (WD) ranges from 10 m to 12 m is widest which 31.7% of total surface area. As a result of the heat map analysis, fish density was highest at right bank under the Gapyeong-bridge, but there was no specific pattern in horizontal distribution of fish. As a result of vertical distribution of fish analysis, 86.6% of fishes are observed at below 6 m of the fish depth (FD, distance from water surface to fish). As a result of the relative height (RH, water depth-distance from bottom to fish ratio) analysis, there was a tendency that fishes are distributed in near surface area more as the WD increased. This tendency could have various reasons such as water temperature gradient along the water depth, and further studies are required for detailed explanation.

• Korean Journal of Ichthyology
• /
• v.33 no.4
• /
• pp.262-272
• /
• 2021

The egg development and early life history of Tribolodon hakonensis that collected from the Seomjin River were studied. The fertilized eggs of the T. hakonensis were slightly adhesive and separated with a yellow yolk and no oil globule. The size of the eggs was an average of 2.89 mm (2.79~2.96) in diameter. Fifty percents of the embryos were hatched in about 63 hrs after fertilization at water temperature of 20℃. The newly hatched larvae showed average 6.92 mm (6.09~7.60) in total length. At 8 days after hatching, they were 13.75±0.67 mm (n=10) in total length and their yolk sacs were completely absorbed, opening mouth and anus (postflexion larvae stage). At 40 days after hatching, they became juvenile and reached 26.99±1.82 mm (n=10) in total length and all their fin rays were formed. At 137 days after hatching, the young fish were 72.63±10.7 mm (n=10) in total length and their body shape, color and behavior were similar to those of adult fish. The size of eggs and the larva after hatching significantly affected the initial growth among groups of genus Tribolodon, and the hatching time of fertilized eggs and the growth rate of the early life history according to the water temperate were different.

• Fisheries and Aquatic Sciences
• /
• v.27 no.3
• /
• pp.145-158
• /
• 2024

Life history characteristics, habitat landscape, and historical events are believed to have shaped the patterns of genetic variation in many taxa. The bony lip barb, Osteohilus vittatus, represent a potamodromous fish that complete all life cycle in freshwater and is widely distributed in Southeast Asia. It usually lives in small rivers and other freshwater habitats, and movement between habitats for either food or reproduction has been typical. These life history characteristics may promote gene flow, leading to less structured populations. However, many freshwater habitats are fragmented, which restricts gene flow. We investigate how this interplay has shaped patterns of genetic variation and phylogeographic structure within this species in the Sundaland, a biodiversity hotspot with a complex geological history, using mitochondrial cytochrome oxidase I (mtCOI) as a genetic marker. Forty-six mtCOI sequences of 506 bp long were collected from ten localities, eight geographically isolated and two connected. The sequences were used for population genetic and phylogeographic analyses. Our results showed a low genetic diversity within populations but high between populations. There was a deep phylogeographic structure among geographically isolated populations but a lack of such structure in the connected habitats. Among geographically isolated populations, sequence divergence was revealed, ranging from 1.8% between Java and Sumatra populations to 12.2% between Malaysia and Vietnam. An indication of structuring was also observed among localities that are geographically closer but without connectivity. We conclude that despite high dispersal capacity, the joint effects of historical events, long-term geographic isolation associated with sea level oscillation during the Pleistocene, and restricted gene flow related to lack of habitat connectivity have shaped the phylogeographic structure within the O. vittatus over the Sundaland.

• Korean Journal of Environmental Biology
• /
• v.27 no.4
• /
• pp.369-374
• /
• 2009

The stumpy bullhead (Pseudobagrus brevicorpus) endemic to Korea is one of critically endangered freshwater fish species. To provide baseline data for its captive breeding and reintroduction we investigated basal characteristics on its growth and sexual maturation in an artificial rearing environment. After 698-day rearing the stumpy bullhead grew up to $89.22{\pm}10.29\;mm$ in total length (TL) and $70.93{\pm}7.68\;g$ in total body weight (BW)($BW=5\times10^{-5}\;TL^{2.678}$; $R^2=0.995$), and showed survival rate of 74.2%. The sex ratio was 1 (471 females) : 1 (473 males), and males ($TL=87.44{\pm}1.07mm$; $BW=8.43{\pm}0.31\;g$) showed significantly faster growth than females ($TL=78.38{\pm}0.92mm$; $BW=6.38{\pm}0.20\;g$) after 663-day rearing. However, condition factor of females was significantly higher than that of males (P<0.05). The number of eggs per female ranged from 508 to 867 (average 734), and their diameters ranged from 0.04 to 1.65 mm. The females which reared about two years in the laboratory condition was able to spawn in the early June after 1~2 days of human chorionic gonadotropin (HCG) injection. The number of hatched fries ranged from 113 to 338 (average 216).

• Korean Journal of Ichthyology
• /
• v.18 no.4
• /
• pp.339-346
• /
• 2006

In this study we conducted artificial hybridization between two sibling species, Rhodeus uyekii and R. notatus, and observed the morphological characteristics in early developmental stage. The two species showed difference in egg shape having rhombus and club respectively. The morphology of yolk of larva just hatched also showed well specific characteristics, that is the bean chaff type for R. uyekii (U type) and anchor type for R. notatus (N type). The rate of fertilization between female R. uyekii and male R. notatus (UN type) and between female R. notatus and male R. uyekii (NU type) were complete and its hatching rate were very high, 71.6% for UN type and 97.5% for NU type. The differences occurred in the yolk shape of hatched larvae for each combination of hybrids. In the group of UN, U type of larvae were found very rarely, but almost all the larvae showed intermediate shape polarized to NN type. Similar phenomenon was observed in the NU having intermediate polarized to UU, but without any NN type. These hybrids will be analyzed for their external morphology, sex ratio, the function of sexual organ and karyology after they grown up to adult.