• Tunnel and Underground Space
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• v.34 no.5
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• pp.503-526
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• 2024

In the research project for the disposal of high-level radioactive waste, where the deep rock environment is considered as the main target for the disposal facilities, the hydrogeological characteristics of rock aquifers are utilized as the most important evaluation factor for the suitability of the disposal site, design/construction of facilities and stability analysis during operation. Such hydrogeological data are obtained by conducting in-situ hydraulic tests using deep boreholes located at the target sites. In this process, the reliability and accuracy of the investigation results are closely linked to various factors including the selection of the optimal testing methods, the performance of testing equipment, the standardization of testing procedures, and data interpretation methods. In this paper, to improve the reliability of the evaluation of hydrogeological characteristics in deep rock aquifers, we conducted a comparative analysis of the hydraulic conductivity characteristics derived from the injection and recovery phases of the most representative hydraulic test, the constant pressure injection test. A high-performance hydraulic testing equipment and standardized testing procedures were applied to deep boreholes in the fractured rock aquifers located in granite/volcanic rock areas in Korea, to obtain the downhole pressure-flow rate, and the hydraulic conductivity was derived by using various transient flow analysis solutions. The results of the study showed a high consistency between the hydraulic conductivity values obtained during the injection and recovery phases within the same test section, even under different permeability conditions (low-permeability/high-permeability). This research case, which precisely conducted a comparative analysis of two different phases (injection/recovery) within a single specific hydraulic testing process using actual field data from deep rock aquifers in Korea, is expected to help overcome the inherent limitations of in-situ field tests, where the validation and verification of measurement results are challenging, and ultimately contribute to enhancing the reliability of deriving in-situ hydrogeological characteristics information.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.19 no.10
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• pp.1-7
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• 2020

In this study, a multichannel gas-liquid microextraction system is designed by integrating the automatic elution of extraction line and multichannel gas-purging liquid phase microextraction. The system uses an injection pump and inert gas to push the extraction solvent to a sample bottle of a gas-phase color autosampler and then implements multichannel gas-liquid microextraction and gas chromatography-mass spectrometry. The system also employs a three-way integrated micro-high-temperature heater, syringe pump, and microflow controller to realize the simultaneous processing of multiple groups of samples, thus improving the sample pretreatment speed and reproducibility and reducing human error. Autoinjection experiments were implemented with polycyclic aromatic hydrocarbon standard samples. The experiments show that the average recovery rate of the system exceeds 70%, and the relative standard among the channels is less than 15%.

• YAKHAK HOEJI
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• v.33 no.3
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• pp.156-160
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• 1989

A gas-chromatographic determination method of thiamin which use a quantitative cleavage of thiamin to 4-methyl-5-(2-hydroxyethyl)thiazol [I] and solvent extraction of the analyte prior to GC injection was modified. A column chromatographic procedure using a reversed phase, high capacity solid phase cartridge was applied to the clean-up of the analyte. Thiazol derivative[I] was quantitatively recovered upon the column method. Acetanilide, an internal standard, has a good recovery through the analytical procedure. The method has analytical precision of 2% or less in the coefficient of variation.

• Korean Journal of Food Science and Technology
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• v.36 no.3
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• pp.518-521
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• 2004

Ginseng saponin was isolated from panax ginseng root using high-speed countercurrent chromatography (HSCCC). Preliminary studies were performed to optimize physical properties of two-phase solvent system and operating parameters including rotation speed of column, elution mode of mobile phase, and flow rate. Two-phase solvent system for isolation of ginseng saponins was composed of chloroform, water, and methanol as blending solvent. Chloroform-aqueous methanol (4:6) systems with various concentration of methanol in water were evaluated for retention of stationary phase in column. Retention of stationary phase decreased with increasing flow rate in tail-to-head elution mode using upper phase as mobile phase and head-to-tail elution mode using lower phase as mobile phase. Latter mode produced high retention at flow rate of 5 mL/min. Optimum conditions for isolation of saponin were chloroform/methanol/water (40/39/21) solvent system; mobile phase, of lower organic layer, flow rate, of 5 mL/min, head to tail elution mode, rotation speed, of 800 rpm, and sample injection, of $200{\mu}L$, Recovery yield of ginseng saponin from panax ginseng root extract by HSCCC was 63.6%, and the purity of HSCCC fractions was verified by TLC.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.7
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• pp.690-698
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• 2010

Contamination of groundwater resources by organic chemicals has become an issue of increasing environmental concern. Surfactant-enhanced aquifer remediation (SEAR) is widely recognized as one of the most promising techniques to remediate organic contaminations in-situ. Solutions of surfactant or surfactant with polymer are used to dramatically expedite the process, which in turn, may reduce the treatment time of a site compared to use of water alone. In the design of surfactant-based technologies for remediation of organic contaminated aquifers, it is very important to have a considerable analysis using extensive numerical simulations prior to full-scale implementation. This study investigated the formation and flow of microemulsions during SEAR of organic-contaminated aquifer using the finite difference model UTCHEM, a three-dimensional, multicomponent, multiphase, compositional model. The remediation process variables considered in this study were the sequence of injection fluids, the injection and extraction rate, the concentrations of polymer in surfactant slug and chase water, and the duration of surfactant injection. For each variable, temporal changes in injection and production wells and spatial distributions of relative saturations in the organic phase were compared. Cleanup time and cumulative organic recovery were also quantified. The study would provide useful information to design strategies for the remediation of nonaqueous phase liquid-contaminated aquifers.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.4
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• pp.321-323
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• 1995

Observations were recorded regarding various fertility parameters on 26 Jersey donor cows following superovulation under tropical conditions. These cows, in their mid-luteal phase were treated with 2,500-3,000 i.u. PMSG or 28-40 mg FSH followed by $500{\mu}g$ $PGF_{2{\alpha}}$ injection 48-60 hours later, to induce oestrus. The cows were bred artificially twelve hours following standing oestrus. Embryo collection was carried out 7 days after oestrus. $PGF_{2{\alpha}}$ was injected to each donor cow after embryo recovery to regress the corpora lutea. Fertility data($PGF_{2{\alpha}}$-Oestrus interval, services per conception, days between embryo collection and successful service and any pathololgical condition) were recorded. $PGF_{2{\alpha}}$-Oestrus interval and correlation (r) between number of corpora lutea and $PGF_{2{\alpha}}$-Oestrus interval were $30.9{\pm}6.3$ and 0.17, respectively. Of 26 treated donors, 19 conceived within a period of $91.7{\pm}18.8$ days after embryo recovery. Average services per conception were $2.3{\pm}0.3$. Only two cows developed metritis which conceived after treatment with antibiotics. These observations indicated no profound adverse effect of superovulation on subsequent reproduction of donor cows, except some effect on services per conception, under tropical conditions.

• Analytical Science and Technology
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• v.36 no.4
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• pp.152-160
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• 2023

Adenosine and cordycepin are bioactive compounds with health benefits. Therefore, both substances are often used to assess the quality of Cordyceps products. Optimization and validation of the HPLC/DAD method for determining two nucleosides were studied. The samples were prepared using an ultrasound-assisted extraction (ultrasonic bath). The result was optimal conditions for aqueous extraction, an extraction time of 35 min, and an extraction temperature of 40 ℃. The Chromatographic separation was achieved using a reverse phase column (InfinityLab Poroshell 120 EC-C18, 4.6 × 250 mm, 2.7 ㎛) at 30 ℃ with a mobile phase gradient elution of water and methanol at a flow rate of 0.7 mL/min. The eluents were monitored via a diode array detector at 260 nm. Two nucleosides were separated by less than 12 min after injection. The developed method was found to be excellent linear (r² > 0.9999), accurate (% recovery 95.34-98.51), and precise (% relative standard deviation < 2.0). The limit of detection (LOD) and quantification (LOQ) were 0.45 and 1.38 mg/mL for adenosine and 0.47 and 1.43 mg/mL for cordycepin, respectively. This method was satisfactory for simultaneously quantitating two nucleoside contents, which were used to evaluate Cordyceps products.

• KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
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• v.5B no.2
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• pp.189-195
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• 2005

This paper presents a new velocity estimation strategy for a non-salient permanent magnet synchronous motor drive without high frequency signal injection or special PWM pattern. This approach is based on the d-axis current regulator output voltage of the drive system, which contains the rotor position error information. The rotor velocity can be estimated through a rotor position tracking PI controller that controls the position error at zero. For zero and low speed operation, the PI gain of the rotor position tracking controller has a variable structure according to the estimated rotor velocity. Then, at zero speed, the rotor position and velocity have sluggish dynamics because the varying gains are very low in this region. In order to boost the bandwidth of the PI controller during zero speed, the loop recovery technique is applied to the control system. The PI tuning formulas are also derived by analyzing this control system by frequency domain specifications such as phase margin and bandwidth assignment.

• Journal of Food Hygiene and Safety
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• v.18 no.2
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• pp.43-50
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• 2003

A liquid chromatographic method, using matrix solid-phase dispersion (MSPD) is developed for the extraction of residual furazolidone in chicken eggs. Blank or fortified egg samples (0.5 g) were blended with Octadecylsilyl (Bulk $C_{18}$, 40${\mu}{\textrm}{m}$, 18%. load, endcapped. 2 g) derivatized silica. After homogenization, $C_{18}$/egg and Na$_2$S $O_4$matrix were transferred to a column made of 10 ml glass syringe and filter paper and compressed 4.0∼4.5 ml volume. The column was washed with 8 ml of hexane and dried under $N_2$ gas. Furazolidone was eluted with acetonitrile (8 ml) under gravity. The eluate containing furazolidone was free from interfering compounds when analyzed by HPLC with UV detection (365 nm, photodiode array). Calibration curves were linear (r = 0.99985) and inter- (1.47%) and intra-assay (5.29%) variabilities for the concentration range examined (7.8∼497 ng/g of eggs, 20 ${mu}ell$ injection volume) were indicative of an acceptable methodology for the analysis of furazolidone. Average recovery of furazolidone added to egg was 96.2%. The limit of detection for the proposed method was 1 ng/g for furazolidone. The method using MSPD is proposed as an alternative assay to the classical method which involves the use of large volumes of a harmful solvent and requires a long tedious separation and clean-up processes prior to its determination.

• Korean Journal of Veterinary Research
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• v.44 no.3
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• pp.367-371
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• 2004

This report describes the determination of ceftiofur residues in milk from treatment of lactating dairy cattle by intramuscular injection of three consecutive daily doses of about 1 mg /kg BW, the recommended label dosing. The separation of ceftiofur was achieved on $C_1_8$ reverse phase column. The mobile phase consisted of 0.1% trifluoracetic acid in water (A) and 0.05% acetic acid in acetonitrile (B) and grediently flowed at the flow rate of 0.4 mL/min. As a result of analysis of blank raw bovine milk samples, matrix interference was not shown. Limit of detection and limit of quantitaion was 0.5 ng/mL and 1 ng/mL, respectively. The values of precision and recovery satisfied the guideline of National Veterinary Research and Quarantine Service (NVRQS, Korea). The mean residual concentration of ceftiofur in milk did not exceed 3.71 ng/mL when ceftiofur was administered intramuscularly to lactating dairy cattle for 3 consecutive days at 1 mg/kg of BW per day. It is much lower than the proposed MRL (100 ng/mL) of ceftiofur in milk.