• Title/Summary/Keyword: Initial population

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Estimation of effective population size using single-nucleotide polymorphism (SNP) data in Jeju horse

  • Do, Kyoung-Tag;Lee, Joon-Ho;Lee, Hak-Kyo;Kim, Jun;Park, Kyung-Do
    • Journal of Animal Science and Technology
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    • v.56 no.8
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    • pp.28.1-28.6
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    • 2014
  • This study was conducted to estimate the effective population size using SNPs data of 240 Jeju horses that had raced at the Jeju racing park. Of the total 61,746 genotyped autosomal SNPs, 17,320 (28.1%) SNPs (missing genotype rate of >10%, minor allele frequency of <0.05 and Hardy-Weinberg equilibrium test P-value of < $10^{-6}$) were excluded after quality control processes. SNPs on the X and Y chromosomes and genotyped individuals with missing genotype rate over 10% were also excluded, and finally, 44,426 (71.9%) SNPs were selected and used for the analysis. The measures of the LD, square of correlation coefficient ($r^2$) between SNP pairs, were calculated for each allele and the effective population size was determined based on $r^2$ measures. The polymorphism information contents (PIC) and expected heterozygosity (HE) were 0.27 and 0.34, respectively. In LD, the most rapid decline was observed over the first 1 Mb. But $r^2$ decreased more slowly with increasing distance and was constant after 2 Mb of distance and the decline was almost linear with log-transformed distance. The average $r^2$ between adjacent SNP pairs ranged from 0.20 to 0.31 in each chromosome and whole average was 0.26, while the whole average $r^2$ between all SNP pairs was 0.02. We observed an initial pattern of decreasing $N_e$ and estimated values were closer to 41 at 1 ~ 5 generations ago. The effective population size (41 heads) estimated in this study seems to be large considering Jeju horse's population size (about 2,000 heads), but it should be interpreted with caution because of the technical limitations of the methods and sample size.

Colonization and Population Changes of a Biocontrol Agent, Paenibacillus polymyxa E681, in Seeds and Roots

  • Park, Okhee;Kim, Jinwoo;Ryu, Choong-Min;Park, Chang-Seuk
    • The Plant Pathology Journal
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    • v.20 no.2
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    • pp.97-102
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    • 2004
  • Paenibacillus polymyxa E681, with its plant growth promotion and root colonization ability, has been proven to be a promising biocontrol agent of cucumber and barley. This study investigated the attributes related to the movement of bacteria from the seed to the radicle and to the whole root system. It also illustrated the existing form and population changes of the bacteria on seed and root using the scanning electron microscope and confocal laser scanning microscopy. The bacteria invaded and colonized the inside of the seed coat while the seeds were soaked in bacterial suspension. Almost the same number of bacteria on seed surface invaded the inside of the seed coat right after seed soaking. The population densities of E681 increased greatly inside as well as on the surface of the seed before the radicle emerged. The bacteria attached on the emerging radicle directly affected the initial population of newly emerg-ing root. The colonized cells on the root were arranged linearly toward the elongation of the root axis. In addition to colonizing the root surface, strain E681 was found inside the roots, where cells colonized the inter-cellular space between certain epidermal and cortical cells. When the cucumber seeds were soaked in bacterial suspension and sown in pot, the bacterial populations attached on both the surface and inside of the root were sustained up to harvesting time. This means that E681 successfully colonized the root of cucumber and sustained its population density up to harvesting time through seed treatment.

Biological aspects and population dynamics of Indian mackerel (Rastrelliger kanagurta) in Barru, Makassar Strait, Indonesia

  • Andi Asni;Hasrun;Ihsan;Najamuddin
    • Fisheries and Aquatic Sciences
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    • v.27 no.6
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    • pp.392-409
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    • 2024
  • The present study aims to analyze the biological aspects and population dynamics of Indian mackerel in Barru waters. Data was collected in Barru for 11 months, from June 2022 to April 2023. The observed parameters of biological aspects included gonadal maturation stages (GMSs), size at first gonadal maturation, and length-weight relationship. Meanwhile, the aspects of population dynamics encompass age group, growth, mortality rate, and exploitation rate. Data analysis consisted of morphological selection of general maturation stages, Spearman-Kärber method in estimating gonadal first maturation size, Bhattacharya method in identifying age group, von Bertalanffy function through FISAT II to measure growth (L and K), Pauly Model to estimate mortality rate, Beverton & Holt Model to estimate Y/R, and virtual population analysis (VPA) analysis to estimate stock and fish yield. The results demonstrated that GMS I was observed to be dominant, followed by stages II and III. The initial gonadal maturation was estimated to be 17.98-19.28 cm (FL) for females and 17.98-19.27 cm (FL) for males. The length-weight relationship in male and female Indian mackerels indicated a positive allometric growth. The mode grouping analysis results from the fork length measurement revealed three age groups. It was also identified that the asymptotic length (L) = 29.5 cm (fork length), growth rate coefficient (K) = 0.46 per year, and theoretical age at zero length (t0) = -0.3576 per year. Total mortality (Z) = 2.67 per year, natural mortality (M) = 1.10 per year, fishing mortality (F) = 1.57 per year, and exploitation rate (E) = 0.59, the actual Y/R = 0.083 gram/recruitment, and optimal Y/R 0.03 gram/recruitment. Fishing mortality is higher than the natural mortality rate, and a high exploitation value (E > 0.5) also reflects over-exploitation. VPA analysis on fish yields and stock estimation reported a highly exploited rate between the 11.5 cm and 14.5 cm length classes and an exceeding current yield of 467.07 tons/year with a recommended yield of 233.53 tons/year to ensure population sustainability.

Development of a Forecasting Model for Bacterial Wilt in Hot Pepper (고추 풋마름병 예찰 모형 개발)

  • Kim, Ji-Hoon;Kim, Sung-Taek;Yun, Sung-Chul
    • Research in Plant Disease
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    • v.18 no.4
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    • pp.361-369
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    • 2012
  • A population density model for bacterial wilt, which is caused by Ralstonia solanacearum, in hot pepper was developed to estimate the primary infection date after overwintering in the field. We developed the model mechansitically to predict reproduction of the pathogen and pathogensis on seedlings of the host. The model estimates the pathogen's populations both in the soil and in the host. In order to quantify environmental infection factors, various temperatures and initial population densities were determined for wilt symptoms on the seedlings of hot pepper in a chamber. Once, the pathogens living in soil multiply up to 400 cells/g of soil, they can infect successfully in the host. Primary infection in a host was supposed to be started when the population of the pathogen were over $10^9$ cells/g of root tissue. The estimated primary infection dates of bacterial wilt in 2011 in Korea were mostly mid-July or late-July which were 10-15 days earlier than those in 2010. Two kinds of meterological data, synoptic observation and field measurements from paddy field and orchard in Kyunggi, were operated the model for comparing the result dates. About 1-3 days were earlier from field data than from synoptic observation.

Population Viability Analysis of a Gold-spotted Pond Frog (Rana chosenica) Population: Implications for Effective Conservation and Re-introduction (금개구리 (Rana chosenica) 개체군의 생존분석: 개체군의 효과적인 보존과 야생복귀를 위한 제안)

  • Cheong, Seok-Wan;Sung, Ha-Cheol;Park, Dae-Sik;Park, Shi-Ryong
    • Korean Journal of Environmental Biology
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    • v.27 no.1
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    • pp.73-81
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    • 2009
  • Population viability analysis of a Gold-spotted pond frog (Rana chosenica) population at Cheongwon-gun, Chungbuk, in South Korea was conducted and we proposed several suggestions for effective conservation and re-introduction of the species. Simulating a developed model over 1,000 times predicted that the population will exist over 30 years with a relatively low growth rate of 0.113, but with a high probability of extinction as 81.1%. Population growth and extinction probability were the most greatly depended on the rate of successful metamorphosis. In the case of outbreak of amphibian diseases such as Chytridiomycosis and Ranavirus, the population will be easily extinct within 4 years with 100% probability. In a habitat of which carrying capacity is 200, to successfully re-introduce an extinct population, it is initially needed to put 100 individuals of which 83% is males and its age structure is normal-distributed. If we additionally conducts artificial supplementation of 10% individuals every 2 years from 4 years to 10 years after initial reintroduction, the population will become a stable with 0.297 growth rate and 0.290 extinction rate. Our results are the first case of amphibian population viability analysis in Korea and could be used to develop effective conservation and re-introduction plans for endangered Gold-spotted pond frog.

Release Strategy for the Red Fox (Vulpes vulpes) Restoration Project in Korea Based on Population Viability Analysis (개체군 생존력 분석을 이용한 여우복원사업 방사전략)

  • Lee, Hwa-Jin;Lee, Bae-Keun;Kwon, Gu-Hui;Chung, Chul-Un
    • Korean Journal of Environment and Ecology
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    • v.27 no.4
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    • pp.417-428
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    • 2013
  • The red fox (Vulpes vulpes), listed as a Class I endangered species by the Ministry of Environment of Korea, has been considered to be extinct in South Korea since the 1980s, and an intensive restoration project has been underway in Sobaeksan national park. This study was carried out to develop a suitable model for the red fox reintroduction program based on Population viability analysis (PVA) by using the VORTEX program. If 10 animals (5 females and 5 males) were continuously released into the initial zero population every year for 10 years, population growth rate and extinction probability over the next 50 years after the introduction of the population were $0.018{\pm}0.204$ and 0.354, respectively; the maximum population size was 116.34 at the 16th year after the first release, and a reduction rate of 1.22 every year from the 17th year was inferred. We found that additional releases would be needed from the 17th year after the initial release to maintain a positive growth rate and to prevent the extinction of the released red foxes, and releasing more than 12 individuals every year would be needed for the long-term, continuous existence of red foxes. By contrast, if fewer than 6 red fox individuals were released the extinction probability over the next 50 years was more than 80%. To maintain the minimum population growth rate, the release of more than 8 individuals were needed for positive population growth. The population growth rate was more stable when 10 animals in the change of their sex rate every year from the set value were released as the female-to- male sex ratio of 6:4 rather than 1:1. However, if the female-biased sex ratio was increased by more than 7:3, a negative population growth was expected. The occurrence rate of roadkill and poaching are important factors in the red fox restoration project. The extinction probability was decreased to 30% if each factor was decreased to 3% based on the standard baseline; however, if each factor was increased to more than 3%, an extinction rate of about 90% was reached over the next 50 years.

Flotation Separation of Biological Floc Using the Dissolved Air Flotation Process (용존공기부상(DAF) 공정을 이용한 생물학적 플록의 부상분리)

  • Kwak, Dong-Heui;Kim, Seong-Jin;Lim, Young-Hwan
    • Journal of Korean Society of Water and Wastewater
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    • v.18 no.5
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    • pp.649-655
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    • 2004
  • The behavior of biological particles in DAF (dissolved air flotation) process was analyzed by employing PBT (Population balance theory). After decline growth phase of activated sludge, the value of the initial collision-attachment efficiency was increased over than 0.35 corresponding relatively high value in the whole life cycle of microorganism. For practical application of DAF as a solid separation process. It is desirable that microbial particles should be operated to perform high solid removal efficiency in biological wastewater treatment.

Evaluation and Isolation of Phytin Phosphohydrolyzing Bacterial Population in the Rumen

  • Suzuki, C.;Ushida, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.7
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    • pp.957-961
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    • 2000
  • A series of experiments was conducted to evaluate phytin phosphohydrolysis actlVlty in the rumen and to isolate phytase positive rumen bacteria. Endogenous phytase activity of wheat bran was estimated and compared with that of bacterial phytin phosphohydrolysis. Substantial phytase activity was detected in wheat bran during in vitro rumen incubation. Bacterial phytase activity was suggested not to be high. Only two facultative anaerobes, Klebsiella sp. and Corynebacterium sp. were isolated as phytase producing organisms. These belonged to a minor microbial group in the rumen population. Protozoal fraction showed an initial velocity of phytin phosphohydrolysis 7 times higher than the bacterial fraction.

Markovian Model Analysis of Influenza System (인플루엔자 유행의 마르코프 모델 해석)

  • 정형환;김권수
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.33 no.11
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    • pp.440-446
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    • 1984
  • This thesis investigates the quantitative aspect of epidemic phenomena utilizing the analytical method of discrete time systems based on the theory of Markov processes. In particular, the pattern on the epidemic character of Influenza was analyzed by the mathematical model of Influenza system, which is derived according to the ecologic relationship between five epidemiolgic states of individuals. The quantitative aspects of the model was characterized by digital computer simulations. The main results were obtained as follows: 1) A Markovian model of influenza system represents accurate spead curve. 2) The latent period of influenza has the standard deviation of 1.98 and also the incubation period is 2.68. 3) If the value of susceptibilities in the pre-epidemic period is less than 20% of the population, the epidemic will occur sporadically. 4) The initial value of susceptibilties obtained by this markov theory is less about 10% of total population than the obtained value according to the deterministic model.

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A Method for Determining the k Most Vital Arcs in Maximum Flow Problem (최대유통문제에서 k-MVA를 결정하는 방법)

  • 정호연
    • Journal of the military operations research society of Korea
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    • v.25 no.2
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    • pp.106-116
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    • 1999
  • The purpose of this study is to present a method for determining the k most vital arcs in the maximum flow problem using genetic algorithms. Generally, the problem which determine the k most vital arcs in maximum flow problem has known as NP-hard. Therefore, in this study we propose a method for determining all the k most vital arcs in maximum flow problem using genetic algorithms. First, we propose a genetic algorithm to find the k most vital arcs removed at the same time and then present the expression and determination method of individuals compatible with the characteristics of the problem, and specify the genetic parameter values of constitution, population size, crossover rate, mutation rate and etc. of the initial population which makes detecting efficiency better. Finally, using the proposed algorithms, we analyzed the performance of searching optimal solution through computer experiment. The proposed algorithms found all alternatives within shorter time than other heuristic methods. The method presented in this study can determine all the alternatives when there exists other alternative solutions.

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