• The korean journal of orthodontics
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• v.49 no.4
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• pp.246-253
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• 2019

Objective: To evaluate the effectiveness of three different caries-preventing agents on artificial caries in a Streptococcus mutans-based caries model. Methods: Sixty-five caries-free human molar enamel blocks were treated with a demineralization solution and a remineralization solution. The specimens were assigned to the following groups according to the caries-protective product applied: group A, chlorhexidine varnish; group B, fluoride-releasing chemically cured sealant; group C, fluoride-releasing lightcured sealant; group D, positive control (specimens that were subjected to de- and remineralization cycles without treatment with any caries-protective agents); and group E, negative control (specimens that were not subjected to de- and remineralization cycles). Samples in groups A-D were stored in demineralization solution with S. mutans and thereafter in artificial saliva. This procedure was performed for 30 days. Average fluorescence loss (${\Delta}F$) and surface size of the lesions were measured using quantitative light-induced fluorescence at baseline and on the 7th, 14th, and 30th days. Results: After 30 days, group A demonstrated a significant increase in ΔF and the surface size of the lesions, no significant difference in comparison with the positive control group, and a significant difference in comparison with the negative control group. Group B showed no significant changes in both parameters at any of the measurement points. While group C showed increased ${\Delta}F$ after 14 days, no significant fluorescence change was observed after 30 days. Conclusions: Both fluoride-releasing sealants (chemically or light-cured) show anti-cariogenic effects, but the use of chlorhexidine varnish for the purpose of caries protection needs to be reconsidered.

• Journal of the korean academy of Pediatric Dentistry
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• v.33 no.4
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• pp.615-623
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• 2006

The purpose of this study was to evaluate the specificity, sensitivity, and diagnostic power of caries activity test using LED fluorescence. The subjects of this study were 55 children of $6{\sim}7$ years old. LED light were irradiated to labial or buccal surface of all teeth. Fluorescence from initial carious lesion of teeth illuminated by an LED light was observed through barrier filter and the number of teeth showing lesion, size and position of lesion were counted. Streptococcus mutans colony counting and dDfFtT rate test were also done and their correlation was compared. And then specificity, sensitivity, diagnostic power of optical caries activity test using LED light were evaluated. 1. There was positive $correlation({\gamma}=0.43)$ between LED fluorescence test and Streptococcus mutans count(P<0.05). 2. When visual examination was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 100%, 76.1%, and 100%. 3. When dDfFtT rate was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 88.9%, 47.8%, and 95.7%. 4. When S. mutans colony counting was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 100%, 58.7%, and 100%. Considering the above results, optical caries activity test using LED light could be regarded as a practical method because of its close relationship with microbiological caries activity test.

• Journal of the korean academy of Pediatric Dentistry
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• v.42 no.3
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• pp.218-225
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• 2015

This study used sodium fluorescein to improve imaging diagnostic ability by increasing the fluorescence difference between sound enamel and caries lesions. It also made it easier to discriminate between stain and caries lesions using quantitative light-induced fluorescence (QLF). Half of the specimen surface was covered with nail varnish as a control. Specimens were divided randomly in six decalcification groups and decalcified for different lengths of time. Then, ${\Delta}F$ was measured using QLF-D. After applying 0.075% sodium fluorescein, we measured ${\Delta}F$ again and compared it with the initial value. After cutting the central portion of the specimen, we measured the lesion depth using scanning electron microscopy. The lesion surfaces observed with QLF were darker than normal enamel, whereas they were lighter than normal enamel after applying fluorescein. Longer decalcification time was associated with greater fluorescent dye penetration. The ${\Delta}F$ measured after applying fluorescein was higher than the initial value (p < 0.05). Due to QLF measurement using fluorescein being more sensitive for diagnosing early decalcification, this approach will enable early diagnosis of dental caries before the cavity formation stage, allowing the treatment of early caries lesions. With QLF and sodium fluorescein, we can easily discriminate between stain and caries lesions.

• Journal of the korean academy of Pediatric Dentistry
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• v.31 no.4
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• pp.671-679
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• 2004

The purpose of this study was to develop a practical caries activity test by fluorescence using laser, plasma light and halogen light. The subjects of study were 45 children of 7-8 years old Argon laser, plasma light and halogen light were irradiated to buccal or labial surface of all teeth. Fluorescence of initial carious lesion from teeth was observed through barrier filter and the number of teeth showing lesion was counted. Visual examination for the dDfFtT, mutans streptococci screening test and Lactobacilli colony counting were also done. Data analysis was accomplished by Axelsson's method. The result from the present study can be summarized as follows. 1. Laser, plasma light and halogen light could detect the initial carious lesions better than visual examination(p<0.05). 2. There was positive correlation between laser(r=0.42), plasma light(r=0.41), halogen light(r=0.39) and dBfFtT rate(p<0.01). 3. The specificity sensitivity and predictive value was showed highest value in laser, but was showed favorable value in plasma light and halogen light. In regard to above results, laser, plasma light and halogen light all considered to be reliable method for determining individual caries activity. And they were also considered to be practical method because it would be simple, inexpensive, and time saving method.

• Journal of dental hygiene science
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• v.13 no.4
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• pp.403-409
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• 2013

The purpose of this study was to evaluate the correlation between caries experience, Quantitative Light-Induced Fluorescence-Digital (QLF-D) redings and new caries activity test (Cariview) results in preschool children. Fifty-seven healthy kindergarten children (male 28, female 29) were participated this study. The calibrated dentist investigated the caries experience of children and new caries activity test. Cariview samples were incubated in the activated medium at $37^{\circ}C$ for 48 hours. All QLF-D taking and readings were performed by one experienced and trained operator under identical conditions in a dental unit chair located in a darkened room. Analysis range was limited to the maxillary and mandibular anterior teeth. QLF-D redings (white spot and dental plaque) were analysed using QLF system. The dft index had a relatively high correlation with the QLF-D redings (white spot: r=0.617, simple plaque score: r=0.500) (p<0.01). Also, there was significant correlation between dft index and the Cariview score (r=0.286) (p<0.05). However, the Cariview score had no significant correlation between dt index and ft index (p>0.05). QLF-D can be evaluated objectively the initial caries lesions and dental plaque correlated with caries experience. Therefore, QLF-D will be useful to the study of caries prediction.

• Restorative Dentistry and Endodontics
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• v.24 no.3
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• pp.495-502
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• 1999

Recently root caries is reported as an increasing oral disease Many researchers studied in vivo and in vitro enamel lesions but due to its peculiar structure and different chemical composition compared to the enamel, little effort has been made to root caries study. The purpose of this study is to compare and to evaluate the effect of pH on the progression of artificial root caries lesion. Under the constant degree of saturation, the specimens were divided into 4 groups(pH 4.3, 5.0, 5.5, 6.0). Each group was immersed in acid buffer solution for 1, 3, 5, 7 days and examined using the polarizing microscope. The results were as follows: 1. The body of the lesion in the dentin showed higher degree of positive birefringence compared to sound dentin. 2. The rate of progression of the lesion slowed as the pH increased. 3. In all groups, the lesion progressed rapidly at the initiation or the experiment but increased gradually as time elapsed. In conclusion, the study shows that the pH in the acid buffer solution influenced the rate of progression of the lesion in artificial root caries. Compared to the previous enamel study, the initial pattern of the lesion progressed rapidly and as time elapsed increased gradually.

• Journal of the korean academy of Pediatric Dentistry
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• v.27 no.1
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• pp.169-179
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• 2000

The purpose of this study is to develop the system which convert the optical difference of teeth texture between intact enamel and incipient caries lesion into shade difference by laser fluorescence and to develop new and simple caries activity test using laser fluorescence. The experimental design of this study consists of three parts. In first part, a new method for the in vitro assessment of changes in initial enamel caries lesion of Bovine teeth using laser fluorescence is tested. In second part, in vivo assessment undertaken. Number of teeth which showed incipient carious lesion on buccal surface examined by laser fluorescence was compared with the caries activity test of $Cariescreen^{(R)}$ test and other oral environmental test of dDfFtT. In third part, new caries activity test measured by laser fluorescence was developed on the basis of above results and evaluated the sensitivity, specificity, and diagnostic power. Optical density measured by laser fluorescence was increased as increasing the depth of incipient carious lesion and showed high correlation$(\gamma=0.7015)$ with lesion depth. Optical density showed direct proportion to lesion depth. Linear equation was obtained between the optical density and the lesion depth by regression analysis. The result of caries activity test with laser fluorescence showed high correlation with those of $Cariescreen^{(R)}$ test and dDfFtT examination. Caries activity test with laser fluorescence showed 48% of sensitivity, 52% of specificity, and 45% of diagnostic power on the basis of dDfFtT examination, and also showed 48% of sensitivity, 51% of specificity, and 36% of diagnostic power on the basis of $Cariescreen^{(R)}$ test. In regard above result, caries activity test with laser fluorescence considered to be reliable for caries activity test compared with other oral environmental test. and it was also considered to be practical because it would be simple, inexpensive, and time saving method.

• Journal of the korean academy of Pediatric Dentistry
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• v.30 no.1
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• pp.1-9
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• 2003

To evaluate the quantitative nature of laser fluorescence(LF) and dye-enhanced laser fluorescence(DELF) on early enamel caries, artificial caries were induced on the bovine enamels and argon laser was irradiated on the surfaces of lesions. Fluorescence radiance from enamel specimen was recorded with CCD camera which was connected to PC image analyzing system. The difference of fluorescence of radiance(DFR) between the carious and the sound enamel in each sample of the LF and the DELF groups were measured by image analyzing program. The DFR of lesion surfaces measured with laser fluorescence and lesion depths were evaluated and the results are as follows: The caries lesions were discriminated from sound enamels by the darkness with LF and by the brightness with DELF. The DELF was more sensitive than the LF at the earlier stage of demineralization.

• Journal of Korean society of Dental Hygiene
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• v.12 no.1
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• pp.27-35
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• 2012

Objectives : This study was performed to provide the basic data for oral health of infants, through the comparison of Streptococcus mutans activities(the representative bacteria of initial dental caries), in main nutritional foods for infants: breast milk, milk powder, and soya milk. Methods : The study targeted 30 infants and the saliva was sampled after the oral examination. The pH of sampled saliva was measured; and the sample of 'the saliva', 'the saliva and breast milk', and 'the saliva and formula milk' by researcher were produced; and the measurement was repeated 7 times. Results : As a result of examining the change of colony number of Streptococcus mutans by hour, the bacterial activity took in the source of nutrition took place in the first place; and in the group with dental caries, breast-feeding at night occurreds most frequently. Due to the combined activities, such as the increase in the contact time, the existence rate of dental caries appeared high. However, as the improvement of breast-feeding is closely related to the nutrition supply to babies, rather than the improvement of breast-feeding, the 12 hours after the culture in which Streptococcus mutans activities started was considered. Conclusions : In case of breast-feeding at night, it is necessary to remove the dental plaque in the mouth, before 12 hours has passed. Especially, using toothbrush and toothpaste to decrease the occurrence of dental caries, after the eruption of teeth, is recommended for oral administration.

• Journal of the korean academy of Pediatric Dentistry
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• v.28 no.4
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• pp.613-619
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• 2001

The aims of this study were to compare the accuracy, sensitivity and specificity of cnventional visual examination, radiography and a new laser fluorescence method, KaVo Diagnodent, for the detection of occlusal caries lesions. One hundred sound human premolars and molars which had no restorations or interproximal cavities were tested by three methods. Tooth lesions depth was assessed at histologic examination using Caries detector dye The following results were obtained. 1. Diagnodent show 7.8 in sound tooth, 25.4 in initial caries, 30.5 in enamel caries, and 53.8 in dentin caries with average score 2. Spearman and Pearson relation coefficient was high between tooth-specimen test with dye and Diagnodent(0.736, 0.619), visual examination(0.664, 0.666), and was low between tooth-specimen test with dye and radiographic examination(P<0.01, total) 3. Accuracy of occlusal caries was highest on Diagnodent(65%) and lowest on radiographic examination(35%) 4. In initial caries, the sensitivity and specificity of Diagnodent method was the highest. In enamel caries, the sensitivity of visual examination was the highest and specificity of Diagnodent method was the highest. In dentinal caries, the sensitivity and specificity of Diagnodent method was the highest and sensitivity of visual examination was the lowest.