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Selection and Antifungal Activity of Antagonistic Bacterium Bacillus subtilis KMU-13 against Cucumber scab, Cladosporium cucumerinum KACC 40576 (검은별무늬병균 Cladosporium cucumerinum KACC 40576에 대한 길항균주 Bacillus subtilis KMU-13의 선발 및 항진균 활성)

  • Park Sung-Min;Lee Jun-Seuk;Park Chi-Duck;Lee Jung-Hun;Jung Hyuck-Jun;Yu Tae-Shick
    • KSBB Journal
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    • v.21 no.1 s.96
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    • pp.42-48
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    • 2006
  • Bacillus subtilis KMU-13 was isolated from the Lillehammer forest soils at Norway and shown a strong antifungal activity on cucumber scab, Cladosporium cucumerinum KACC 40576. B. subtilis KMU-13 produced a maximum level of antifungal substance under incubation aerobically at $30^{\circ}C$, 180 rpm for 48 hours in LB broth containing 0.5% maltose and 0.5% bactopeptone and initial pH adjusted to 6.0. Butanol extract of cultured broth was confirmed inhibitory zone by plate assay and Rf 0.64 value substance by thin layer chromatography (TLC) represented high antifungal activity against C. cucumerinum KACC 40576 and also shown fungal growth inhibitory activity against Botytis cinerea KACC 40573, C. gloeosporioides KACC 40804, D. byoniae KACC 40669, F. oxysporum KACC 40037, F. oxysporum KACC 40052, F. oxysporum f. sp. radicis-lycopersici KACC 40537, F. oxysporum KACC 40902, M. cannonballus KACC 40940, P. cambivora KACC 40160, R. soiani AG-1 KACC 40101, R. solani AG-4 KACC 40142, and S. scleotiorum KACC by agar diffusion method.

Differences between Patients with TB-Destroyed Lung and Patients with COPD Admitted to the ICU

  • Seo, Young-Kyeong;Lee, Chae-Hun;Lee, Hyun-Kyung;Lee, Young-Min;Park, Hye-Kyeong;Choi, Sang-Bong;Kim, Hyun-Gook;Jang, Hang-Jea;Yum, Ho-Kee;Lee, Seung-Heon
    • Tuberculosis and Respiratory Diseases
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    • v.70 no.4
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    • pp.323-329
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    • 2011
  • Background: Although patients with tuberculous-destroyed lung (TDL) account for a significant proportion of those with chronic airflow obstruction, it is difficult to distinguish patients with airway obstruction due to TDL from patients with pure chronic obstructive pulmonary disease (COPD) on initial presentation with dyspnea. We investigated clinical features differing between (i) patients with TDL and airway obstruction and (ii) those with COPD admitted to the intensive care unit (ICU) due to dyspnea. Methods: We reviewed the medical records of patients with TDL who had a forced expiratory volume in 1 second (FEV1)/forced vital capacity (FVC) of <70% on a pulmonary function test (PFT; best value closest to admission) and patients with COPD without a history of pulmonary tuberculosis (TB) who were admitted to the ICU. Ultimately, 16 patients with TDL and 16 with COPD were compared, excluding patients with co-morbidities. Results: The mean ages of the patients with TDL and COPD were 63.7 and 71.2 years, respectively. Mean FVC% (50.4% vs. 71.9%; p<0.01) and mean FEV1% (39.1% vs. 58.4%; p<0.01) were significantly lower in the TDL group than in the COPD group. More frequent consolidation with TB (68.8% vs. 31.3%; p=0.03) and more tracheostomies (50.0% vs. 0.0%; p=0.02) were observed in the TDL than in the COPD group. Conclusion: Upon ICU admission, patients with TDL had TB pneumonia more frequently, more diminished PFT results, and more tracheostomies than patients with COPD.

A Study on Estimation of Initial Gas in Place for Coalbed Methane Field Using Production Data at Canada (생산자료를 이용한 캐나다 CBM 원시부존량 평가 연구)

  • Seo, Hyeongjun;Moon, Bryan;Kim, Kihong;Han, Jungmin;Kwon, Sunil
    • Journal of the Korean Institute of Gas
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    • v.22 no.1
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    • pp.64-77
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    • 2018
  • This paper presents the prediction of the original gas in place(OGIP) by using the material balance method and decline curve analysis method with production history and pressure transient test data for four coalbed methane wells in the Horseshoe Canyon field. In this study, the conventional gas equation and the Jensen and Smith(J&S) equation were used to material balance analysis, and the Arps' empirical correlation and Khaled method were applied to decline curve analysis. From the results, the OGIP estimated from the conventional gas and the J&S method was small in difference as under 12%. Also, in case of decline curve analysis, it was found that the Khaled method has appropriated to calculate the OGIP, because the OGIP was estimated as unlimited value by the Arps' equation from the decline exponent of 1 - 3.5. The OGIP difference between conventional gas method and Khaled method was calculated as 8.67% ~ 31.04%, and those between J&S method and Khaled method was 13.67% ~ 26.49%.

Lightweight Design of an Outer Tie Rod Using Meta-Model Based Optimization Technique (메타모델기반최적화를 이용한 아우터타이로드의 경량화 설계)

  • Kim, Young-Jun;Park, Soon-Hyeong;Lee, Kwon-Hee;Park, Young-Chul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.11
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    • pp.7754-7760
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    • 2015
  • The outer tie rod is one of the part of steering system, the optimization process was executed to find the lightweight design. The inner tie rod was considered in the optimum design of an outer tie rod. it could be closer to the test condition than in the case of considering outer tie rod only. The aluminum forging material was considered as a weight reduction proposal. The target of optimization was the shape of the minimum weight to resist at the load of buckling. RSM and Kriging interpolation method were applied as a optimization method to consider the nonlinear shape optimization problem. Then, 16.3%, 16.6% of weight reduction was obtained from the result comparing with that of the initial model. The results of meta model optimization was compared with that of finite element method. The error values of buckling load estimation were 2.6%, 2.04%. and those of weight estimation were 0.17%, 0.13%. Therefore, it seemed that the result of Kriging model could be obtained closer to optimum value than that of RSM model.

Benchmarking of US General Contractor's Pre-construction Services for a CM at Risk Project to Improve Contractor's Competitiveness (책임형 CM사 경쟁력 확보 및 선진화를 위한 미국 건설사의 시공이전단계 서비스 벤치마킹 연구)

  • Lee, Chang-Jae;Lee, Sang-Hyo;Ahn, Yong-Han
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.21 no.3
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    • pp.9-18
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    • 2017
  • Construction Management at Risk (CMAR) is a project delivery method that enables CM companies to deliver projects at a Guaranteed Maximum Price (GMP). General contractors can apply CMAR from the initial design phase right through the construction phase to reduce risks and improve project performance. One of the major advantages CMAR offers is that it permits a general contractor to provide a comprehensive suite of preconstruction services, including estimating, a constructability review, value engineering, drawings and a specification review, green building, and Building Information Modeling(BIM), among others. However, general contractors in South Korea currently provide only limited preconstruction services using CMAR because few CMAR projects have yet been implemented in Korea and their experience using the method is therefore limited. This benchmark study of how foreign general contractors utilize CMAR in their projects, particularly during the preconstruction process, its purpose, and the roles and responsibilities of each of the different participants in successful implementations thus provides invaluable information and will serve as a useful guide for Korean contractors seeking to incorporate CMAR preconstruction services in their projects and thus improve the competitiveness of their construction businesses.

Application of Rare Earth Compounds for the Treatment of Phosphate and Fluoride in Wastewater (인산염 및 불소폐수 처리제로서의 희토류 화합물 적용에 관한 연구)

  • Kim, Jin-Wha;Shin, Sung-Hye;Song, Hye-Won;Kim, Dong-Su;Woo, Sang-Mo;Kwon, Young-Shik
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.6
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    • pp.1127-1137
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    • 2000
  • Applications of lanthanum ion and crude rare earth chloride to the phosphate wastewater and fluorine wastewater, respectively, as treatment agents were studied. For the investigation of phosphate removal characteristics according to the amount of lanthanum ion, initial phosphate content was decreased by about 50% when molar ratio of [$La^{3+}$]:[$PO_4{^{3-}}-P$] was 0.25 and nearly all of phosphate was removed when the molar ratio of [$La^{3+}$]:[$PO_4{^{3-}}-P$] to be doubled. The removal of phosphate by $La^{3+}$ appeared to reach equilibrium state rapidly, and it was exothermic reaction since the removed amount of phosphate was diminished somewhat when the reaction temperature was increased. The zeta potential of combined particulate compound of lanthanum ion and phosphate was located for its isoelectric point at pH 5.5 and the turbidity of treated wastewater was found to vary according to the pH in a similar manner as the absolute value of zeta potential of the combined particulate compound did. For the treatment of fluorine wastewater by crude rare earth chloride, the remaining fluorine content after treatment decreased as the dosage of crude rare earth chloride increased. Whereas, the turbidity of treated wastewater and the amount of sludge generated were shown to increase as more crude rare earth chloride was added. The remaining fluorine content and the turbidity of treated wastewater were decreased and the amount of sludge generated was observed to increase according the increase of coagulant dosage under the condition of constant input of crude rare earth chloride.

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Emergy Carrying Capacity of Sungap-do, An Uninhabited Island in Korea (무인도서 선갑도의 에머지 환경수용력 평가)

  • Kang, Dae-Seok
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.13 no.1
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    • pp.60-67
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    • 2010
  • For sustainable use of the resources of uninhabited islands of Korea, their ecological economic potential needs to be fully integrated into their management policy and the carrying capacity of the islands should be evaluated before using or developing them. The emergy methodology was used to evaluate the ecological economic value and carrying capacity of Sungap-do which is an uninhabited island in Incheon, Korea. The system boundary for the emergy evaluation of the island included the sea area within 1km from the high tide level, following the management boundary for the uninhabited islands of Korea stipulated in the Law on the Conservation and Management of Uninhabited Islands. The total renewable emergy input to Sungap-do was $1.04{\times}10^{20}$ sej/yr from tidal energy. The annual ecological economic contribution of the island was evaluated high at 29.9 billion Em₩/yr. If Sungap-do were developed to the national average, its carrying capacity was 6,586 persons at the current living standard of Korea. The carrying capacity of Sungap-do for the long-term sustainability scenario was 2,337 persons at the same living standard as in the developed scenario. When only emergy contribution of the land area was considered, the carrying capacity of Sungap-do sharply decreased to 14 persons for the developed scenario and 5 persons for the long-term sustainability scenario. The carrying capacity of the uninhabited islands of Korea including Sungap-do, thus, needs to be considered from the initial stage of utilization or development projects to sustain the ecosystem benefits and their sustainable uses.

Effects of Supplemental Vegetable Oils on Ruminal Characteristics, Digestibility and Formation of Fatty Acid Soaps in Sheep (식물성유 첨가가 면양의 반추의 발효특성과 소화율 및 지방산염 형성에 미치는 영향)

  • Kim, D.I.;Choi, J.R.;Cho, S.H.;Lee, D.H.;Chung, T.Y.
    • Journal of Animal Science and Technology
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    • v.46 no.5
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    • pp.783-798
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    • 2004
  • This experiment were conducted to determine the formation of fatty acid soaps, digestibility and ruminal characteristics when vegetable oils were added. Three Corriedale${\times}$ 3 Latin square design. Sheep were fed one of three diets consisting of alfalfa hay and concentrates in a ratio of 70:30. Dietary treatments were 1) no oil, 2) 7% soybean oil and 3) 7% corn oil. At the end of each experimental period, rumen contents from each sheep were collected before feeding and at 1, 3, 6 and 9h after feeding. pH decreased up to the 3 hour after feeding and increased until the end of the collection(p<0.0001). Whereas the concentration of $NH_3$-N showed inverse changes to pH(p<0.0001). Type of oil did not affect the molar concentration of total VFA, acetate, propionate and butyrate. Acetic/propionic acid (A/P) ratio decreased with increasing time(P<0.0001), but the ratio at the end of the experiment became similar to the initial value. No effects were found in the digestibility of dry matter, total-N, crude fiber, ash, NFE, NDF and ADF. The formation of fatty acid soaps(FAS) increased after feeding than 1 hour before feeding(P<0.05) and increased in 7% oils than no oil addition(P<0.05). Long chain fatty acids formed mainly FAS. ince the formation of FAS might decrease negative effects on ruminal haracteristics and total tract digestibility.

Reevaluation of the Effect of Phenobarbital on the Response to Pain in Rat (백서에서 동통에 미치는 Phenobarbital 효과의 재평가)

  • So, Byoung-Gyoum;Kim, Kee-Won;Ko, Myoung-Kyu;Yang, Won-Mo;Cho, Kyu-Park
    • The Korean Journal of Pharmacology
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    • v.22 no.2
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    • pp.88-95
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    • 1986
  • Clinically, subhypnotic doses of barbiturates have been known to elicit hyperalgesia. In this experiment, effect of acute or chronic phenobarital treatment on the response to pain in rat was reevaluated by hot-plate method. To elucidate its mechanism, changes of ${\beta}-endorphin$ contents and [3H]-morphine binding of the rat midbrain as well as functional opiate receptor in vas deferens were also measured. Intraperitoneal injection of sub anesthetic dose phenobarbital induced initial hyperalgesia followed by successive analgesia, while chronic phenobarbital-treatment decreased reactivity to pain. Naloxone (10mg/kg, i.p.) markedly shortened hot plate latency period, and significantly inhibited the analgesic action of phenobarbital. Single dose of phenobarbital did not affect ${\beta}-endorphin$ contents and [3H]-morphine binding in rat mid brain, but in the chronic phenobarbital-treated groups, ${\beta}-endorphin$ contents was increased, while Bmax of opiate receptor binding was decreased. Moreover, very significant correlations among responses to pain, changes of ${\beta}-endorphin$ contents and opiate receptor binding were observed. However, Kd values of opiate receptor bindings were not changed in all preparations. In the chronic phenobarbital-treated vas deferens preparations, ID50 of morphine was increased witb concomittant decrease of maximum effect. But $pA_2 $, value for naloxone was not changed. From these results, it is suggested that phenobarbital can produce analgesia due to changes of ${\beta}-endorphin$ contents as well as functional opiate receptors by receptor regulation.

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Study on the Evapotranspiration of Crisphead Lettuce by the Weighing Lysimeter (Weighing Lysimeter에 의한 결구상치의 증발산량 조사연구)

  • 김시원;김선주;노희수
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.28 no.4
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    • pp.41-48
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    • 1986
  • This study was fulfilled by the weighing lysimeter method at the experimental farm of KonKuk University from April to June of 1986 to investgate the amount of evapotranspiration ( ET-lettuec )by the growing periods, evapotranspiration ratio, amount of watering per one time, days of intermission and soil moisture extraction pattern of the crisphead lettuce cultivated in the clay loam soil by different watering points of pFl.7, pF2.O, pF2.7. The results obtained are summar ized as follows : 1.The total evapotranspiration(ETlettuce) of the pFl.7 treatment plot was 358,9mm., 314.9mm in the pF2.O plot and 281.8mm in the pF2.7 plot, therefore the total ETlettuec increased with the difference of 33mm-44mm by the decrease of watering point. 2.The daily maximum ETlettuce by the watering points was 7.66mm, 6:54mm, 5.98mm, respectively in the last ten days of May, and the mean daily ETlettuce during the growing season by the watering points of pFl.7, pF2.O and pF2.7 was 5.44mm(384.5g), 4.77mm(337.2g) and 4.27mm(301.8g), respectively. 3.The evapotranspiration ratio showed maximum value in the middle of May which was the beginning of mid-season stage, and the mean evapotranspiration ratio during the total growing period was 1A7, 1.29, 1.15 by the watering points. 4.The days of watering intermission by the watering points of pFl.7, pF2.O and pF2.7 was 1.0day, 2.9days and 12.Sdays, respectively. 5.The yield of the crisphead lettuce by the watering treatments showed very high significance, and the pF2.O was confirmed as a optimum watering point. 6.The soil moisture extraction pattern(SMEP) of the pF2.0 treatment plot in the initial stage was 85.6% in the 1st and 2nd soil layer and 14.4% in the 3rd and 4th layer, and in the midseason stage, the moisture extraction proportion of the under layer accounted for 34.7%which showed that the root elongated to the lowest soil layer, and there was no difference of the SMEP between the mid-season and late-season stage. 7.The correlation coefficient between the ETlettuce and yield of lettuce by the three watering points was.739, which showed the significance of 5%.

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