• Title/Summary/Keyword: Initial Design Step

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Production and Characterization of Multi-Polysaccharide Degrading Enzymes from Aspergillus aculeatus BCC199 for Saccharification of Agricultural Residues

  • Suwannarangsee, Surisa;Arnthong, Jantima;Eurwilaichitr, Lily;Champreda, Verawat
    • Journal of Microbiology and Biotechnology
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    • v.24 no.10
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    • pp.1427-1437
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    • 2014
  • Enzymatic hydrolysis of lignocellulosic biomass into fermentable sugars is a key step in the conversion of agricultural by-products to biofuels and value-added chemicals. Utilization of a robust microorganism for on-site production of biomass-degrading enzymes has gained increasing interest as an economical approach for supplying enzymes to biorefinery processes. In this study, production of multi-polysaccharide-degrading enzymes from Aspergillus aculeatus BCC199 by solid-state fermentation was improved through the statistical design approach. Among the operational parameters, yeast extract and soybean meal as well as the nonionic surfactant Tween 20 and initial pH were found as key parameters for maximizing production of cellulolytic and hemicellulolytic enzymes. Under the optimized condition, the production of FPase, endoglucanase, ${\beta}$-glucosidase, xylanase, and ${\beta}$-xylosidase was achieved at 23, 663, 88, 1,633, and 90 units/g of dry substrate, respectively. The multi-enzyme extract was highly efficient in the saccharification of alkaline-pretreated rice straw, corn cob, and corn stover. In comparison with commercial cellulase preparations, the BCC199 enzyme mixture was able to produce remarkable yields of glucose and xylose, as it contained higher relative activities of ${\beta}$-glucosidase and core hemicellulases (xylanase and ${\beta}$-xylosidase). These results suggested that the crude enzyme extract from A. aculeatus BCC199 possesses balanced cellulolytic and xylanolytic activities required for the efficient saccharification of lignocellulosic biomass feedstocks, and supplementation of external ${\beta}$-glucosidase or xylanase was dispensable. The work thus demonstrates the high potential of A. aculeatus BCC199 as a promising producer of lignocellulose-degrading enzymes for the biomass conversion industry.

Real-Time Seam Tracking System Using a Visual Device with Vertical Projection of Laser Beam (레이저빔 수직투사 구조의 시각장치를 이용한 실시간 용접선추적 시스템)

  • Kim, Jin-Dae;Lee, Jeh-Won;Shin, Chan-Bai
    • Journal of the Korean Society for Precision Engineering
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    • v.24 no.10
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    • pp.64-74
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    • 2007
  • Because of the size and environment in the shipbuilding process, the portable type robot is required for the automatic seam tracking. For this reason, the structure of laser sensor should be considered in the initial design step and the coordinate transformation between welding robot and laser sensor, which is joint finder, must be identified exactly and the real time tracking algorithm based on these consideration could be developed. In this research, laser displacement sensor in which its structure is laser beam's vertical projection, is developed to recognize the location of weld joint. In practical applications, however, images of weld joints are often degraded because of the surface specularity or spatter. To overcome the problem, the constrained joint finding algorithm is proposed. In the approach of coordinate conversion rule for the visual feedback control among welding torch, robot body and laser sensor is applied by the same reference point method. In the real time seam tracking algorithms we propose constrained sampling method which uses look ahead distance. The RLS(Recursive Least Square) filter is applied to obtain the smooth tracking path from the sensitive edge data. From the experimental results, we could see the possibility that the developed laser sensor with proposed processing algorithm and real time seam tracking method can be used as a welding under the shipbuilding condition.

A Study on the Methodology of Bioregional Approach for Coastal Area Management - Focus on the Case of Bioregional Classification in the Bay of Hampyong - (연안지역관리를 위한 생물지리지역 접근방법에 관한 연구 - 함평만의 생물지리지역 구분사례를 중심으로 -)

  • Kim, Kwi-Gon;Cho, Dong-Gil;Jung, Sung-Eun;Shin, Ji-Young
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.3 no.3
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    • pp.20-28
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    • 2000
  • The objective of this study is to establish a methodology of bioregional approach for coastal area management as a basis for planning and design. Focusing on the bioregional approach, this study reviewed currently prevailing approaches such as watershed approach and ecological unit approach for planning and management purposes. This research placed its geographical focus on the landward watershed of the Bay of Hampyong located in Chonnam Province, dealing efficiently with shortcomings of existing researches which mainly covered seaward tidal flats without considering outside effects. The main methods of the study are classified into indoor computerized map analysis and field work. For computer analysis, printed maps and digital maps have been analysed, and GIS techniques have been utilized for its synthesis and finalizations. Field work included on-site landscape analysis and verification of a tentative place unit boundary. As a practical step, criteria for classifying bioregion were presented and the selected criteria included : topography & water ways ; roads & administrative boundaries ; habitat types ; and visual enclosure. First, based on the data of topography and water ways, broad classification work was performed and corrections were made based on data drawn out from other criteria. A tentative place unit map was drawn and revised through field visits. This study encompassed an initial but integral part for bioregional approach in landward watershed management of a coastal area. As results of the study, the necessity and efficiency of bioregional approach which considers environmental and cultural components systematically have been presented.

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Development of a Rigid-ended Beam Element and Its Application to Simplify 3-Dimensional Analysis of Bracketed Frame Structures (강체 단부 보요소의 개발 및 브라켓이 있는 골조 구조의 3차원 해석 단순화를 위한 적용)

  • Seo, Seung Il;Lim, Seong Joon
    • Journal of the Society of Naval Architects of Korea
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    • v.34 no.3
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    • pp.76-84
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    • 1997
  • At the initial design stage, for rapid evaluation of strength of ship structures, finite element analysis using beam elements is carried out in general. In beam modeling of ship structures, brackets are usually represented by rigid elements to simplify the analysis. Extent of rigid ends, which is called as a span point, can be determined from the three kinds of view points, i.e., bending, shearing and axial deformation. In this paper, a 2-dimensional novel beam element is developed and a method to replace the 3-dimensional analysis with 2-dimensional analysis is proposed. The developed novel beam element named rigid-ended beam element can consider the effect of three kinds of span points within one element, which was impossible in modeling with the ordinary beam element. Calculated results for the portal frame using the rigid-ended beam element agree with the results using membrane elements. And also, the proposed semi 3-dimensional analysis method which includes two step analysis using influence coefficients shows good accuracy. Structural analysis using the rigid-ended beam element and the semi 3-dimensional method is revealed to have good computing efficiency due to unnecessity of elements corresponding to the brackets and simplification of 3-dimensional analysis.

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Numerical Calculation Method for Paraxial Zoom Loci of Complicated Zoom Lenses with Infinite Object Distance by Using Gaussian Bracket Method (가우스 괄호법을 이용한 무한 물점을 갖는 복잡한 줌 렌즈의 수치해석적인 근축광선 줌 궤적 추적법)

  • Yoo, Nam-Jun;Kim, Won-Seob;Jo, Jae-Heung;Ryu, Jae-Myung;Lee, Hae-Jin;Kang, Geon-Mo
    • Korean Journal of Optics and Photonics
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    • v.18 no.6
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    • pp.410-420
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    • 2007
  • We theoretically derive the set of utilizable paraxial zoom locus equations for all complicated zoom lens systems with infinite object distance, such as a camera zoom lens, by using the Gaussian bracket method and the matrix representation of paraxial ray tracing. And we make the zoom locus program according to these equations in Visual Basic. Since we have applied the paraxial ray tracing equations into Gaussian bracket representation, the resultant program systematically simplifies various constraints of the zoom loci of various N group types. Consequently, the solutions of this method can be consistently used in all types of zoom lens in the step of initial design about zoom loci. Finally, in order to verify the usefulness of this method, we show that one example among 4 groups and that among 5 groups, which are very complex zoom lens systems, can be rapidly and with versatility traced through various interpolations by using this program.

Study on the Determination of Fatigue Damage Parameter for Rubber Component under Multiaxial Loading (다축하중이 작용하는 방진고무부품 피로손상 파라미터 결정에 관한 연구)

  • Moon, Seong-In;Woo, Chang-Su;Kim, Wan-Doo
    • Elastomers and Composites
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    • v.47 no.3
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    • pp.194-200
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    • 2012
  • Rubber components have been widely used in automotive industry as anti-vibration components for many years. These subjected to fluctuating loads, often fail due to the nucleation and growth of defects or cracks. To prevent such failures, it is necessary to understand the fatigue failure mechanism for rubber materials and to evaluate the fatigue life for rubber components. The objective of this study is to develop the durability analysis process for vulcanized rubber components, which is applicable to predict fatigue life at initial product design step. The determination method of nonlinear material constants for FE analysis was proposed. In order to investigate the applicability of the commonly used damage parameters, fatigue tests and corresponding finite element analyses were carried out and strain energy density was proposed as the fatigue damage parameter for rubber components. The fatigue analysis for automotive rubber components was performed and the durability analysis process was reviewed.

Analysis of Variables Influencing the Pressure Build-up and Volume Expansion of Kimchi Package (김치포장의 압력 및 부피 변화에 영향을 미치는 인자의 분석)

  • 이동선;최홍식;박완수
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.2
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    • pp.429-437
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    • 1999
  • A mathematical model was established for estimating changes in pressure and volume of permeable kimchi packages. The model comprises the CO2 gas production from kimchi and permeation of O2, CO2 and N2 through the permeable film or sheet. Using the developed model, the effects of various packaging variables on the pressure and volume changes were analyzed for rigid and flexible packag es of kimchi(3% salt content) at 15oC, and then effect of storage temperature was also looked into. In case of rigid pack of 400g, using the plastic sheet of high CO2 permeability and initial vacuumizing can help to relieve the problem of pressure build up. The lower fill weight can further reduce the pressure, but will result in higher packaging cost. For the flexible package of 3 kg, highly permeable films such as low density polyethylene(LDPE) and polypropylene can reduce the volume expansion. Higher ratio of CO2 permeability to O2 and N2 permeabilities are effective in reducing the volume expansion. Increased surface area cannot contribute to reduction of volume expansion for highly permeable flexible packages of kimchi. For the impermeable packages, pressure and volume at over ripening stage (acidity 1.0%) increase with decreased temperature, while those at optimum ripening stage(acidity 0.6%) change little with temperature. Pressure of permeable rigid LDPE package increases with tem perature at any ripening stage, and temperature affects the volume of flexible LDPE package very slightly. Experimental verification of the present results and package design with economical consid eration are needed as a next step for practical application.

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Design of Water Gas Shift Reactor for Rapid Start-Up in 200 W Portable Fuel Cell System (200 W급 휴대용 연료전지 시스템의 빠른 기동 특성을 위한 수성 가스 반응기 설계)

  • Choi, Jong-Rock;Lee, Sungchul
    • Korean Chemical Engineering Research
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    • v.51 no.4
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    • pp.455-459
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    • 2013
  • The fuel processor for the portable fuel cell includes multi-step processes consisting of hydrogen generator, heat generator and several CO clean-up stages. One of requirements of the fuel processor for portable fuel cell system is a rapid start-up time. Especially, the warm-up time for WGS reactor is crucial factors for total start-up time. In this paper, active heating protocol, which is the heating protocol of WGS reactor supplied by the oxidation of CO rich reformate in the initial stage, is used for a rapid start-up. The air stream fed to the inlet of WGS reactor rapidly oxidize the CO rich reformate in the WGS reactor. Therefore, CO concentration in reformate quickly stabilized at the desired concentration without CO surges.

A Study on the Presumption of Proper Construction Cost of Distribution Facilities by Analyzing Actual Construction Cost (실적공사비 분석을 통한 유통시설물의 적정공사비 추정에 관한 연구)

  • Go, Seong-Seok;Kim, Hyun-Sik;Lee, Hyun-Chul
    • Korean Journal of Construction Engineering and Management
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    • v.9 no.3
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    • pp.108-117
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    • 2008
  • The appearance of the large distribution facility of large enterprise putting first reaches get to the various effect until change of leisure life and life pattern of the consumers from the distribution industry of the interior of a country. Competition of the distribution facility upgrade of the distribution facility and it shows the aspect which becomes the semi-department store, and construction cost is appearing different in proportion to each form or scale. Therefore, purpose of this study was to facilitate amicable construction progress between the owner and the builder through estimating the proper construction cost. This study investigated and analyzed the actual cost of 15 domestic distribution facilities and these datums were used to estimate the proper construction cost. This cost shows that from new project accomplishment through analysis of prediction construction cost for feasibility study from initial plan and design step and can be utilizable elementary data bH decision method to whether or not to propriety of distribution facilities business.

Mixing Zone Analysis on Outfall Plume considering Influence of Temperature Variation (수온 변화의 영향을 고려한 방류관 플룸의 혼합역 분석)

  • Kim Ji-Yeon;Lee Joong-Woo
    • Journal of Navigation and Port Research
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    • v.28 no.10 s.96
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    • pp.947-953
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    • 2004
  • As a large scale port development in coastal waters proceeds step by step and populations in the vicinity of port are getting increased, the issue on 'how to dispose the treated municipal water and wastewater in harbor' brings peoples' concern. The submarine outfall system discharges the primary or secondary treated effluent at the coastline or in deep water, or between these two. The effluent, which has a density similar to that of fresh water, rises to the sea surface forming plume or jet, together with entraining the surrounding sea water and becomes very dilute. We intended in this paper to investigate the impact on dilution of effluent and the behavior of flume under the conditions of the seasonal and spatial temperature variations, which have not been noticeable in designing effective marine outfall system. To predict and analyze the behaviour and dilution characteristics of plume not just with the effluent temperature, but also with the seasonal variation of temperature of surround water, CORMIX( Cornell Mixing Zone Expert System)-GI have been applied. The results should be used with caution in evaluation the mixing zone characteristics of discharged water. We hope to help for the effective operation of outfall system, probable outfall design, protection of water quality, and warm water discharges from a power plant, etc.