• Molecules and Cells
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• 제44권9호
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• pp.627-636
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• 2021

The three-dimensional organization of chromatin and its time-dependent changes greatly affect virtually every cellular function, especially DNA replication, genome maintenance, transcription regulation, and cell differentiation. Sequencing-based techniques such as ChIP-seq, ATAC-seq, and Hi-C provide abundant information on how genomic elements are coupled with regulatory proteins and functionally organized into hierarchical domains through their interactions. However, visualizing the time-dependent changes of such organization in individual cells remains challenging. Recent developments of CRISPR systems for site-specific fluorescent labeling of genomic loci have provided promising strategies for visualizing chromatin dynamics in live cells. However, there are several limiting factors, including background signals, off-target binding of CRISPR, and rapid photobleaching of the fluorophores, requiring a large number of target-bound CRISPR complexes to reliably distinguish the target-specific foci from the background. Various modifications have been engineered into the CRISPR system to enhance the signal-to-background ratio and signal longevity to detect target foci more reliably and efficiently, and to reduce the required target size. In this review, we comprehensively compare the performances of recently developed CRISPR designs for improved visualization of genomic loci in terms of the reliability of target detection, the ability to detect small repeat loci, and the allowed time of live tracking. Longer observation of genomic loci allows the detailed identification of the dynamic characteristics of chromatin. The diffusion properties of chromatin found in recent studies are reviewed, which provide suggestions for the underlying biological processes.

• 품질경영학회지
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• 제49권4호
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• pp.551-567
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• 2021

Purpose: The purpose of this paper is to present implications by analyzing research trends on smart factories by text analysis and visual analysis(Comprehensive/ Fields / Years-based) which are big data analyses, by collecting data based on previous studies on smart factories. Methods: For the collection of analysis data, deep learning was used in the integrated search on the Academic Research Information Service (www.riss.kr) to search for "SMART FACTORY" and "Smart Factory" as search terms, and the titles and Korean abstracts were scrapped out of the extracted paper and they are organize into EXCEL. For the final step, 739 papers derived were analyzed using the Rx64 4.0.2 program and Rstudio using text mining, one of the big data analysis techniques, and Word Cloud for visualization. Results: The results of this study are as follows; Smart factory research slowed down from 2005 to 2014, but until 2019, research increased rapidly. According to the analysis by fields, smart factories were studied in the order of engineering, social science, and complex science. There were many 'engineering' fields in the early stages of smart factories, and research was expanded to 'social science'. In particular, since 2015, it has been studied in various disciplines such as 'complex studies'. Overall, in keyword analysis, the keywords such as 'technology', 'data', and 'analysis' are most likely to appear, and it was analyzed that there were some differences by fields and years. Conclusion: Government support and expert support for smart factories should be activated, and researches on technology-based strategies are needed. In the future, it is necessary to take various approaches to smart factories. If researches are conducted in consideration of the environment or energy, it is judged that bigger implications can be presented.

• Journal of Veterinary Science
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• 제22권1호
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• pp.2.1-2.10
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• 2021

Background: Diseases related to cerebrospinal fluid flow, such as hydrocephalus, syringomyelia, and Chiari malformation, are often found in small dogs. Although studies in human medicine have revealed a correlation with cerebrospinal fluid flow in these diseases by magnetic resonance imaging, there is little information and no standard data for normal dogs. Objectives: The purpose of this study was to obtain cerebrospinal fluid flow velocity data from the cerebral aqueduct and subarachnoid space at the foramen magnum in healthy beagle dogs. Methods: Six healthy beagle dogs were used in this experimental study. The dogs underwent phase-contrast and time-spatial labeling inversion pulse magnetic resonance imaging. Flow rate variations in the cerebrospinal fluid were observed using sagittal time-spatial labeling inversion pulse images. The pattern and velocity of cerebrospinal fluid flow were assessed using phase-contrast magnetic resonance imaging within the subarachnoid space at the foramen magnum level and the cerebral aqueduct. Results: In the ventral aspect of the subarachnoid space and cerebral aqueduct, the cerebrospinal fluid was characterized by a bidirectional flow throughout the cardiac cycle. The mean ± SD peak velocities through the ventral and dorsal aspects of the subarachnoid space and the cerebral aqueduct were 1.39 ± 0.13, 0.32 ± 0.12, and 0.76 ± 0.43 cm/s, respectively. Conclusions: Noninvasive visualization of cerebrospinal fluid flow movement with magnetic resonance imaging was feasible, and a reference dataset of cerebrospinal fluid flow peak velocities was obtained through the cervical subarachnoid space and cerebral aqueduct in healthy dogs.

• 한국의류산업학회지
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• 제21권3호
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• pp.277-287
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• 2019

This study analyzes the online issues of design innovation by a fashion creative director. The study selected fashion house Gucci as the main subject and analyzed social media posts. As for study methods, a social matrix program Textom 2.0 collected 13,014 nouns and adjectives using 'Gucci Collection' as a search keyword from Naver Blogs from March to August 2014 and from March to August 2016. Design related issues were derived through semantic network analysis using Ucinet6 and the NetDraw program. The results of the keyword frequency analysis showed that social media user interest for the Gucci collection increased based on the rapid increase in the number of posts from 1,064 to 2,126 after changing the fashion creative director. The results of visualization of semantic network analysis and content analysis also showed that the main issues related to the Gucci collection design changed after the replacement of the fashion creative director. The study found that issues formed around the product information worn by celebrities for promotion purposes during the 2014 period; however, during the 2016 period, issues were formed around 'vintage' and 'retro' runway concepts with design styles related to Alessandro Michele, the new creative director.

• 한국인터넷방송통신학회논문지
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• 제18권6호
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• pp.1-9
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• 2018

현재 이슈가 되고 있는 4차 산업혁명에서 다양한 빅데이터 기술들을 통하여 기존의 느린 속도 보다 빠른 분석 결과를 즉각적으로 받아 볼 수 있고, 모바일과 웹에서 실시간 모니터링을 하는 연구를 진행하였다. 먼저 IoT 기기인 Raspberry Pi를 이용하여 다양한 비정형 센서 데이터를 생성하고 센서 데이터를 실시간 수집하고, 수집한 데이터를 여러 개의 노드를 이용해 분산 저장한 뒤 저장된 센서 데이터를 가공, 정제 처리하여 분석 모델 및 알고리즘을 통해 분석 결과를 시각화하여 출력한다. 이러한 방법들을 이용한 진행은 IoT를 이용한 빅데이터 및 모바일 관련 분야에서 필요한 고급 인력을 양성 및 데이터를 효율적이고 빠르게 처리할 수 있다. 또한, 실시간 모니터링을 통하여 연구결과의 신뢰성을 확인할 수 있는 정보를 제공하고자 한다.

• 디지털융복합연구
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• 제17권8호
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• pp.373-378
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• 2019

다양한 문화와 문명 속에서 예술가들은 새로운 무언가를 창작하기 보다는 고전의 아름다움을 재해석하고 현대에 맞게 정의 하는 새로운 발상을 시도하고 있다. 즉, 현대의 문화 트렌드는 넘쳐나는 많은 정보들 속에서 고전의 감성과 예술가의 현대적 감각이 합쳐지는 새로운 문화가 수행되고 있는 시점이다. 본 연구는 이러한 문화의 변화에 맞춰 다양한 문화와 디자인의 결합을 시도하고자한다. 소설 이효석의 메밀꽃 필 무렵에 나타난 개인의 심리적 정서, 불가능한 체험의 세계를 표현한 특징을 바탕으로 현실과 상상 속 이분화 된 세계의 경계 부분인 불완전한 의식 영역은 사고와 잠재적인 세계로서 독자로 하여금 상상력을 일으키며, 이러한 문학적 서정성은 새로운 문화를 형성하는 분야이다. 또한 시각화하기 좋은 언어의 구성으로 연구자는 소설 속에서의 언어를 바탕으로 그 속에 내재되어 있는 상징적, 심상적 아름다움을 디자인의 소재로 활용하여 조형분석 하고자 한다.

• Journal of Contemporary Eastern Asia
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• 제18권1호
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• pp.7-29
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• 2019

This study describes an approach for comparative bibliometric analysis of scientific publications related to (i) individual or several departments comprising a university, and (ii) broader integrated subject areas using multiple disciplinary schemes. It uses a custom dataset of scientific publications (ca. 15,000 articles and reviews, published during 2009-2013, and recorded in the Web of Science Core Collections) with author affiliations to the research departments, dedicated to science, technology, engineering, mathematics, and medicine (STEMM), of a comprehensive university. The dataset was subjected, at first, to the department level and discipline level analyses using the newly available KAKEN-L3 classification (based on MEXT/JSPS Grants-in-Aid system), hierarchical clustering, correspondence analysis to decipher the major departmental and disciplinary clusters, and visualization of the department-discipline relationships using two-dimensional stacked bar diagrams. The next step involved the creation of subsets covering integrated subject areas and a comparative analysis of departmental contributions to a specific area (medical, health and life science) using several disciplinary schemes: Essential Science Indicators (ESI) 22 research fields, SCOPUS 27 subject areas, OECD Frascati 38 subordinate research fields, and KAKEN-L3 66 subject categories. To illustrate the effective use of the science mapping techniques, the same subset for medical, health and life science area was subjected to network analyses for co-occurrences of keywords, bibliographic coupling of the publication sources, and co-citation of sources in the reference lists. The science mapping approach demonstrates the ways to extract information on the prolific research themes, the most frequently used journals for publishing research findings, and the knowledge base underlying the research activities covered by the publications concerned.

• 스마트미디어저널
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• 제10권2호
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• pp.110-118
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• 2021

4차 산업혁명의 핵심 성장 동력인 사물인터넷 (IoT) 기술은 자율 형으로 상호 소통하며 공간과 상황에 따른 데이터를 복합처리 할 수 있는 단계로 성장하였다. 이에 따라 IT 인프라 구조는 점점 복잡해지고 시스템을 안정적으로 유지하기 위한 모니터링 분야의 중요도가 높아지고 있다. 모니터링 기술은 과거에도 사용되었지만, 빠르게 변하는 ICT 기술에 대응할 수 있는 유연한 모니터링 시스템을 모색할 필요성이 있다. 본 논문은 오픈소스 기반 Prometheus 모니터링 시스템을 설계하고 테스트 하는 연구를 진행한다. IoT 디바이스를 기반으로 간단한 인프라를 구축하고 Exporter를 통해 디바이스에 대한 데이터를 수집한다. Prometheus는 데이터를 Pull 기반으로 수집한 뒤 Grafana를 사용하여 하나의 대시보드에 통합하고 데이터를 시각화함으로써 디바이스에 대한 정보를 모니터링 할 수 있다.

• Archives of Plastic Surgery
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• 제48권3호
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• pp.323-328
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• 2021

Background Lymphaticovenular anastomosis (LVA) is a minimally invasive surgical procedure used to treat lymphedema. Volumetric measurements and quality-of-life assessments are often performed to assess the effectiveness of LVA, but there is no method that provides information regarding postoperative morphological changes in lymphatic vessels and veins after LVA. Photoacoustic lymphangiography (PAL) is an optical imaging technique that visualizes the distribution of light-absorbing molecules, such as hemoglobin or indocyanine green (ICG), and provides three-dimensional images of superficial lymphatic vessels and the venous system simultaneously. In this study, we performed PAL in lymphedema patients before and after LVA and compared the images to evaluate the effect of LVA. Methods PAL was performed using the PAI-05 system in three patients (one man, two women) with lymphedema, including one primary case and two secondary cases, before LVA. ICG fluorescence lymphography was performed in all cases before PAL. Follow-up PAL was performed between 5 days and 5 months after LVA. Results PAL enabled the simultaneous visualization of clear lymphatic vessels that could not be accurately seen with ICG fluorescence lymphography and veins. We were also able to observe and analyze morphological changes such as the width and the number of lymphatic vessels and veins during the follow-up PAL after LVA. Conclusions By comparing preoperative and postoperative PAL images, it was possible to analyze the morphological changes in lymphatic vessels and veins that occurred after LVA. Our study suggests that PAL would be useful when assessing the effect of LVA surgery.

• Animal Bioscience
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• 제34권1호
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• pp.134-142
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• 2021

Objective: To understand the athletic characteristics of Thoroughbreds, high-throughput analysis has been conducted using horse muscle tissue. However, an in vitro system has been lacking for studying and validating genes from in silico data. The aim of this study is to validate genes from differentially expressed genes (DEGs) of our previous RNA-sequencing data in vitro. Also, we investigated the effects of exercise-induced stress including heat, oxidative, hypoxic and cortisol stress on horse skeletal muscle derived cells with the top six upregulated genes of DEGs. Methods: Enriched pathway analysis was conducted using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) tool with upregulated genes in horse skeletal muscle tissue after exercise. Among the candidates, the top six genes were analysed through geneMANIA to investigate gene networks. Muscle cells derived from neonatal horse skeletal tissue were maintained and subjected to exercise-related stressors. Transcriptional changes in the top six genes followed by stressors were investigated using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results: The inflammation response pathway was the most commonly upregulated pathway after horse exercise. Under non-cytotoxic conditions of exercise-related stressors, the transcriptional response of the top six genes was different among types of stress. Oxidative stress yielded the most similar expression pattern to DEGs. Conclusion: Our results indicate that transcriptional change after horse exercise in skeletal muscle tissue strongly relates to stress response. The qRT-PCR results showed that stressors contribute differently to the transcriptional regulation. These results would be valuable information to understand horse exercise in the stress aspect.