• Title/Summary/Keyword: Infection of rice seedlings

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Occurrence of Bakanae Disease by Fusarium moniliforme Associated with Different Seedling Methods (묘대(苗代) 양식(樣式)과 Fusarium moniliforme에 의한 벼키다리병(病)의 발생(發生))

  • Sung, Jae-Mo;Yang, Sung-Suk
    • The Korean Journal of Mycology
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    • v.13 no.1
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    • pp.23-26
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    • 1985
  • Percentage of Bakanae infection of rice seedlings grown in nursery box was higher than rice seedlings grown in protected semi-irrigated nursery regardless of seed treatment. Rice seedlings grown in nursery box had higher Bakanae symptom ratio than that of the rice seedlings grown in protected semi-irrigated nursery in the paddy field conditions after transplanting. Grain yield of rice transplanted from nursery box was lower than that of the rice in protected semi-irrigated nursery.

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Overexpression of a Rice Diacylglycerol Kinase Gene OsBIDK1 Enhances Disease Resistance in Transgenic Tobacco

  • Zhang, Weidong;Chen, Jie;Zhang, Huijuan;Song, Fengming
    • Molecules and Cells
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    • v.26 no.3
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    • pp.258-264
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    • 2008
  • A rice diacylglycerol kinase (DGK) gene, OsBIDK1, which encodes a 499-amino acid protein, was cloned and characterized. OsBIDK1 contains a conserved DGK domain, consisting of a diacylglycerol kinase catalytic subdomain and a diacylglycerol kinase accessory subdomain. Expression of OsBIDK1 in rice seedlings was induced by treatment with benzothiadiazole (BTH), a chemical activator of the plant defense response, and by infection with Magnaporthe grisea, causal agent of blast disease. In BTH-treated rice seedlings, expression of OsBIDK1 was induced earlier and at a higher level than in water-treated control seedlings after inoculation with M. grisea. Transgenic tobacco plants that constitutively express the OsBIDK1 gene were generated and disease resistance assays showed that overexpression of OsBIDK1 in transgenic tobacco plants resulted in enhanced resistance against infection by tobacco mosaic virus and Phytophthora parasitica var. nicotianae. These results suggest that OsBIDK1 may play a role in disease resistance responses.

Systemic Infection of Maize, Sorghum, Rice, and Beet Seedlings with Fumonisin-Producing and Nonproducing Fusarium verticillioides Strains

  • Dastjerdi, Raana;Karlovsky, Petr
    • The Plant Pathology Journal
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    • v.31 no.4
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    • pp.334-342
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    • 2015
  • Two fumonisin-nonproducing strains of Fusarium verticillioides and their fumonisin producing progenitors were tested for aggressiveness toward maize, sorghum, rice, and beetroot seedlings grown under greenhouse conditions. None of the plants showed obvious disease symptoms after root dip inoculation. Fungal biomass was determined by species-specific real-time PCR. No significant (P = 0.05) differences in systemic colonization were detected between the wild type strains and mutants not producing fumonisins. F. verticillioides was not detected in any of the non-inoculated control plants. The fungus grew from roots to the first two internodes/leaves of maize, rice and beet regardless of fumonisin production. The systemic growth of F. verticillioides in sorghum was limited. The results showed that fumonisin production was not required for the infection of roots of maize, rice and beet by F. verticillioides.

Production and Transplanting of Ectomycorrhizal Pine Seedlings Using the Old Fairy Ring of Tricholoma matsutake (기존 송이 균환(菌環)을 이용한 송이균 감염 소나무의 생산 및 이식)

  • Ka, Kang-Hyeon;Hur, Tae-Chul;Park, Hyun;Kim, Hee-Su;Bak, Won-Chull;Yoon, Kap-Hee
    • Journal of Korean Society of Forest Science
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    • v.95 no.6
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    • pp.636-642
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    • 2006
  • To make a new fairy ring of Tricholoma matsutake in situ, the way of production and transplanting of ectomycorrhizal seedlings of T. matsutake using Pinus densiflora was investigated after transplanting from 2000 to 2005 as well as the method to improve their survival rate for the fungus. For the production of ectomycorrhizal pine seedlings, the seedlings planted at the edge of fairy ring of T. matsutake in November showed 97% of the survival rate, while those planted in April showed 80% of the rate. For the complete infection of the T. matsutake, it required more than two years after planting. The infection rate of mycelia for the ectomycorrhizal seedlings was 17.6% when the natural seedlings were used, whereas it was relatively low when the seedlings prepared from the nursery were used. The survival of T. matsutake mycelium reached up to 22% by the transplanting in April, while the mycelium transplanted in October and November showed less than 5% of the survival. The survival of T. matsutake on the transplanted seedlings was the highest in the seedlings having 50% of infection rate before transplanting. Excavation of the ectomycorrhizal seedling to examine the vitality of ectomycorrhizal roots of T. matsutake resulted in the perishing of them. Therefore, the method using a 'rice bag triers' to check living mycelium of T. matsutake without digging of transplanted seedlings was introduced in this study. In addition, it is recommended that the examination has to be conducted at least two years after transplanting.

Seed-born Burkholderia glumae Infects Rice Seedling and Maintains Bacterial Population during Vegetative and Reproductive Growth Stage

  • Pedraza, Luz Adriana;Bautista, Jessica;Uribe-Velez, Daniel
    • The Plant Pathology Journal
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    • v.34 no.5
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    • pp.393-402
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    • 2018
  • Rice world production is affected due to the growing impact of diseases such as bacterial panicle blight, produced by Burkholderia glumae. The pathogen-induced symptoms include seedling rot, grain rot and leafsheath browning in rice plants. It is currently recognized the entrance of this pathogen to the plant, from infected seeds and from environmental sources of the microorganism. However, it is still not fully elucidated the dynamics and permanence of the pathogen in the plant, from its entry until the development of disease symptoms in seedlings or panicles. In this work it was evaluated the infection of B. glumae rice plants, starting from inoculated seeds and substrates, and its subsequent monitoring after infection. Various organs of the plant during the vegetative stage and until the beginning of the reproductive stage, were evaluated. In both inoculation models, the bacteria was maintained in the plant as an endophyte between $1{\times}10^1$ and $1{\times}10^5cfu$ of B. $glumae.g^{-1}$ of plant throughout the vegetative stage. An increase of bacterial population towards initiation of the panicle was observed, and in the maturity of the grain, an endophyte population was identified in the flag leaf at $1{\times}10^6cfu$ of B. $glumae.g^{-1}$ fresh weight of rice plant, conducting towards the symptoms of bacterial panicle blight. The results found, suggest that B. glumae in rice plants developed from infected seeds or from the substrate, can colonize seedlings, establishing and maintaining a bacterial population over time, using rice plants as habitat to survive endophyticly until formation of bacterial panicle blight symptoms.

Overwintering of the Pathogen and Factors Affecting Disease Develpoment of Rice Brown Spot Caused by Cochliobolus miyabeanus (벼깨씨무늬병균의 월동과 발병조건에 관한 연구)

  • Yeh, Wan-Hae;Lee, Hoon-Seop;Kim, Yong-Ki;Shim, Hong-Sik;Jee, Hyeong-Jin;Nam, Ki-Woong
    • Research in Plant Disease
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    • v.10 no.2
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    • pp.112-116
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    • 2004
  • This study was carried out to investigate factors affecting disease development of rice brown spot caused by Cochliobolus miyabeanus in the field and under the greenhouse conditions. In normal paddy fields, the pathogen was more frequently isolated from dried rice straws than wet straws after overwintering. The pathogen was also readily isolated from wet rice straws of salty paddy fields although infection rate of the fields was low. The disease symptom was first observed at 2 to 3days after inoculation when the seedlings were kept for 6 hours in dew chamber. The number of lesions peaked at 14 hr incubation in cultivars Chucheongbyeo and 24 hr incubation in cultivar Daesanbyeo and Tetep. Sixty three-d-old seedlings were more susceptible than 21 to 49-d-old seedlings regardless of cultivar. There was some difference in number of lesions observed, but the lesions were evenly spread regardless of leaf position. Light on the rice seedlings at 3,000 lux or 6,000 lux showed more severe symptoms than those in the dark.

Studies on Pathogenicity of Wheat Scab Fungus(Gibberella zeae) to Various Crop Seedlings

  • Chung H. W.;Chung H. S.;Chung B. J.
    • Korean journal of applied entomology
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    • v.3
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    • pp.21-25
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    • 1964
  • 1. Purpose of this experiment is to investigate the influence of wheat scab fungus(Gibberella zeae) remaining in soil or residues of infected plants to the growth of the seedlings of 14 different crops. 2. Susceptibility of these crops to the infection by G. zeae was investigated by planting these crops both to the artificially inoculated soils and naturally infected soils where both serious and light wheat scab epidemic have occurred previously. 3. In artifical inoculation tests, bailey, wheat, rye, soybean, rice, buckwheat, corn, cotton and greenbean has shown susceptible reaction, while indianbean, sesame, sorghum, chinese cabbage and radish has shown resistant reaction. 4. In artificial inoculation tests, there was significant difference in the germination ratio of the susceptible crops between the plants planted in inoculated soils and uninoculated soils with the exception of rice, com and cotton. Preemergence seedling blight was confined only to barley and corn, whereas postemergence seedling blight was confined to rye, wheat, rice buckwheat, barley and corn. la most of the susceptible crops secondary roots were almost rotted and the primary roots were either partially rotted or discolorated in inoculated soil. There was significant difference in the stem length of the plants grown in inoculated soils and uninoculated soils in susceptible crops. 5. No infection by G. zeae was observed when wheat, barley and rye plants were sown to the soils where both serious and light wheat scab epidemic occurred naturally.

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Occurrence of Rice Seedling Blight Caused by Rhizopus sp. in Direct-Seedling Dry Paddy Field and Its Effects on the Later Growth of Rice (건답직파 벼 논에서 Rhizopus sp.에 의한 모마름병 발생과 감염정도가 벼 후기생육에 미치는 영향)

  • 강수웅;권진혁;정부근;박창석
    • Korean Journal Plant Pathology
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    • v.11 no.1
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    • pp.94-97
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    • 1995
  • In 1994, an abnormal rice growth was observed in a 0.5-ha scale farmer's field located at Chogye-Myeon, Hapcheon-Gun, Gyeongnam Province where rice cv. Hwayeongbyeo was seeded directly in dry paddy field conditions. The major symptoms were less stands per acreage, short leaf length, and hypertrophy of root tip portion. The disorder was identified as rice seeding bight caused by Rhizopus sp. The average plant stand per m2 in the infested field was 108, while it was 375 in the normal field, and leaf growth was retarded to less than one-third of healthy plant. The average leaf lengths of infected and healthy seedlings were 12.8cm and 38.9cm, respectively. When the infected seedlings were transplanted to pots, flooded-soil conditions developed no new root growth but upland conditions allowed 11.2 new roots emerging. The length of newly emerged root in infested soil was 5.3cm in upland and 7.1cm in intermediate flooding conditions. However, it was 10.9cm in non-infested soil with intermediate flooding conditions. When the plants were matured, the stem length of infected plants was reduced slightly as compared to normal plants, whereas the length of panicle was not significantly different between infected and healthy plants. The number of panicle per plant, however, greatly different with variation of infection degree. Grain quality such as the number of complete and incomplete grains per panicle, the complete grain weight per panicle, and the weight of 1000 grains was not significantly different.

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Dravya: a Putative Organic Treatment against Alternaria padwickii Infection in Paddy

  • Sathyanarayana, S.G.;Lokesh, S.;Vasanth Kumar, T.;Shetty, H.S.
    • Animal cells and systems
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    • v.10 no.1
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    • pp.21-25
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    • 2006
  • In the present study, Dravya-an organic compound used for seed treatment along with the common fungicides to test its compatibility in the management of Alternaria padwickii in paddy. Dravya (a sea weed extract) was found highly compatible with fungicides like Bavistin and Dithane M-45. Incidence of Alternaria padwickii and Bipolaris oryzae was also reduced to a greater extent in the paddy seed samples of Dravya treatment. On the other hand, it also enhanced the seed germination and seedling vigour. Seedlings of treated samples also showed enhanced activity of peroxidase upon challenge inoculation with Alternaria padwickii. The enzyme activity in the seedlings challenged with the pathogen was two fold more over control. The suppression in disease incidence in growing plants indicated the promising effect of Dravya and Dithane M-45 under green- house conditions.

Morphological Characters and Seed Transmission of Bipolaris panici-miliacei Causing Leaf Spot of Common Millet (기장 점무늬병균 (Bipolaris panici-miliacei)의 형태적 특징과 종자전염)

  • 이두형
    • Korean Journal Plant Pathology
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    • v.13 no.1
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    • pp.18-21
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    • 1997
  • Bipolaris panici-miliacei, Cercospora fusimaculans, Fusarium moniliforme and Rhizoctonia solani were pathogenic fungi detected from 5 seed samples of common millet (Panicum miliaceum). Morphological characters of B. panici-miliacei were as follows. Conidiophores were dark olivaceous brown, simple, cylindrical, geniculate, and septate. Conidia were fusoid, dark olivaceous brown, tapering gradually toward the ends, straight to slightly curved, 3~13 distoseptate, and 29.4~155.4$\times$10~26 ${\mu}{\textrm}{m}$ in size with dark hilum included within the contour of the basal cell. Seed infection with B. panici-miliacei caused seed rotting, coleoptile spot, and seedling blight of common millet plants. According to the inoculation experiments, B. panici-miliacei showed strong virulence on the young seedlings of common millet, but very weak virulence on the young seedlings of rice (Oryza sativa) and foxtail millet (Setaria italica).

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