Browse > Article
http://dx.doi.org/10.5423/PPJ.OA.05.2015.0088

Systemic Infection of Maize, Sorghum, Rice, and Beet Seedlings with Fumonisin-Producing and Nonproducing Fusarium verticillioides Strains  

Dastjerdi, Raana (Seed and Plant Improvement Research Institute (SPII))
Karlovsky, Petr (Molecular Phytopathology and Mycotoxin Research, Georg-August-University Gottingen)
Publication Information
The Plant Pathology Journal / v.31, no.4, 2015 , pp. 334-342 More about this Journal
Abstract
Two fumonisin-nonproducing strains of Fusarium verticillioides and their fumonisin producing progenitors were tested for aggressiveness toward maize, sorghum, rice, and beetroot seedlings grown under greenhouse conditions. None of the plants showed obvious disease symptoms after root dip inoculation. Fungal biomass was determined by species-specific real-time PCR. No significant (P = 0.05) differences in systemic colonization were detected between the wild type strains and mutants not producing fumonisins. F. verticillioides was not detected in any of the non-inoculated control plants. The fungus grew from roots to the first two internodes/leaves of maize, rice and beet regardless of fumonisin production. The systemic growth of F. verticillioides in sorghum was limited. The results showed that fumonisin production was not required for the infection of roots of maize, rice and beet by F. verticillioides.
Keywords
Fusarium verticillioides; fumonisins; maize;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Castella, G., Bragulat, M. R. and Cabanes, F. J. 1999. Surveillance of fumonisins in maize-based feeds and cereals from Spain. J. Agric. Food Chem. 47:4707-4710.   DOI
2 da Silva, J. B., Pozzi, C. R., Mallozzi, M. A., Ortega, E. M. and Correa, B. 2000. Mycoflora and occurrence of aflatoxin B1 and fumonisin B1 during storage of Brazilian sorghum. J. Agric. Food Chem. 48:4352-4356.   DOI
3 Demeke, T. and Jenkins, G. R. 2010. Influence of DNA extraction methods, PCR inhibitors and quantification methods on real-time PCR assay of biotechnology-derived traits. Anal. Bioanal. Chem. 396:1977-1990.   DOI
4 Desjardins, A. E., Munkvold, G. P., Plattner, R. D. and Proctor, R. H. 2002. FUM1-A gene required for fumonisin biosynthesis but not for maize ear rot and ear infection by Gibberella moniliformis in field tests. Mol. Plant-Microbe Interact. 15:1157-1164.   DOI
5 Desjardins, A. E., Manandhar, H. K., Plattner, R. D., Manandhar, G. G., Poling, S. M. and Maragos, C. M. 2000. Fusarium species from Nepalese rice and production of mycotoxins and gibberellic acid by selected species. Appl. Environ. Microbiol. 66:1020-1025.   DOI
6 Desjardins, A. E. and Plattner, R. D. 2000. Fumonisin B1-nonproducing strains of Fusarium verticillioides cause maize (Zea mays) ear infection and ear rot. J. Agric. Food Chem. 48:5773-5780.   DOI
7 Desjardins, A. N., Plattner, R. D., Nelsen, T. C. and Leslie, J. F. 1995. Genetic analysis of fumonisin production and virulence of Gibberella fujikuroi mating population A (Fusarium moniliforme) on maize (Zea mays) seedlings. Appl. Environ. Microbiol. 61:79-86.
8 dos Reis, T. A., Zorzete, P., Pozzi, C. R., da Silva, V. N., Ortega, E. and Correa, B. 2010. Mycoflora and fumonisin contamination in Brazilian sorghum from sowing to harvest. J. Sci. Food Agric. 90:1445-1451.   DOI
9 Gilchrist, D. G. 1998. Programmed cell death in plant disease: the purpose and promise of cellular suicide. Annu. Rev. Phytopathol. 36:393-414.   DOI
10 Glenn, A. E., Zitomer, N. C., Zimeri, A. M., Williams, L. D., Riley, R. T. and Proctor, R. H. 2008. Transformation-mediated complementation of a FUM gene cluster deletion in Fusarium verticillioides restores both fumonisin production and pathogenicity. Mol. Plant-Microbe Interact. 21:87-97.   DOI
11 Han, Z., Tangni, E. K., Huybrechts, B., Munaut, F., Scauflaire, J., Wu, A. and Callebaut, A. 2014. Screening survey of co-production of fusaric acid, fusarin C, and fumonisins B1, B2 and B3 by Fusarium strains grown in maize grains. Mycotoxin Res. 30:231-240.   DOI
12 Hanson, L. E. and Hill, A. L. 2004. Fusarium species causing fusarium yellows of sugarbeet. J. Sugar Beet Res. 41:163-178.   DOI
13 Horne, E. C., Kumpatla, S. P., Patterson, K. A., Gupta, M. and Thompson, S. A. 2004. Improved high-throughput sunflower and cotton genomic DNA extraction and PCR fidelity. Plant Mol. Biol. Rep. 22:83a-83i.   DOI
14 Hornunga, R. W. and Reeda, L. D. 1990. Estimation of average concentration in presence of nondetectable values. Appl. Occup. Environ. Hyg. 5:46-51.   DOI
15 Jardine, D. J. and Leslie, J. F. 1999. Aggressiveness to mature maize plants of Fusarium strains differing in ability to produce fumonisin. Plant Dis. 83:690-693.   DOI
16 Kedera, C. J., Leslie, J. F. and Claflin, L. E. 1992. Systemic infection of corn by Fusarium moniliforme. (Abstr.) Phytopathology 82:1138.
17 Kushiro, M., Nagata, R., Nakagawa, H. and Nagashima, H. 2008. Liquid chromatographic deletion of fumonisins in rice seeds. Rep. Nat'l. Food Res. Inst. 72:37-44.
18 Logrieco, A., Mule, G., Moretti, A. and Bottalico, A. 2002. Toxigenic Fusarium species and mycotoxins associated with maize ear rot in Europe. Eur. J. Plant Pathol. 108:597-609.   DOI
19 Lamprecht, S. C., Marasas, W. F. O., Alberts, J. F., Cawood, M. E., Gelderblom, W. C. A., Shephard, G. S., Thiel, P. G. and Calitz, F. J. 1994. Phytotoxicity of fumonisins and TA-toxin to corn and tomato. Phytopathology 84:383-391.   DOI
20 Leslie, J. F., Plattner, R. D., Desjardins, A. E. and Klittich, C. J. R. 1992. Fumonisin B1 production by strains from different mating populations of Gibberella fujikuroi (Fusarium section Liseola). Phytopathology 82:341-345.   DOI
21 Logrieco, A., Moretti, A., Ritieni, A., Bottalico, A. and Corda, P. 1995. Occurrence and toxigenicity of Fusarium proliferatum from preharvest maize ear rot, and associated mycotoxins in Italy. Plant Dis. 79:727-731.   DOI
22 Maheshwar, P. K., Moharram, S. A. and Janardhana, G. R. 2009. Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR). Braz. J. Microbiol. 40:134-138.   DOI
23 Mikusova, P., Srobarova, A., Sulyok, M. and Santini, A. 2013. Fusarium fungi and associated metabolites presence on grapes from Slovakia. Mycotoxin Res. 29:97-102.   DOI
24 Mule, G., Susca, G. S. and Moretti, A. 2004. A species-specific PCR assay based on the calmodulin partial gene for identification of Fusarium verticillioides, F. proliferatum and F. subglutinans. Eur. J. Plant Pathol. 110:495-502.   DOI
25 Munitz, M. S., Resnik, S. L., Pacin, A., Salas, P. M., Gonzalez, H. H. L., Montti, M. I. T., Drunday, V. and Guillin, E. A. 2014. Mycotoxigenic potential of fungi isolated from freshly harvested Argentinean blueberries. Mycotoxin Res. 30:221-229.   DOI
26 Munkvold, G. P., McGee, D. C. and Carlton, W. M. 1997. Importance of different pathways for maize kernel infection by Fusarium moniliforme. Phytopathology 87:209-217.   DOI
27 Munkvold, G. P. 2003. Epidemiology of Fusarium diseases and their mycotoxins in maize ears. Eur. J. Plant Pathol. 109:705-713.   DOI
28 Munkvold, G. P. and Carlton, W. M. 1997. Influence of inoculation method on systemic Fusarium moniliforme infection of maize plants grown from infected seeds. Plant Dis. 81:211-216.   DOI
29 Munkvold, G. P. and Desjardins, A. E. 1997. Fumonisins in maize: can we reduce their occurrence. Plant Dis. 81:556-565.   DOI
30 Nelson, P. E., Desjardins, A. E. and Plattner, R. D. 1993. Fumonisins, mycotoxins produced by Fusarium species: biology, chemistry, and significance. Annu. Rev. Phytopathol. 31:233-252.   DOI
31 Nutz, S., Doell, K. and Karlovsky, P. 2011. Determination of the LOQ in real-time PCR by receiver operating characteristic curve analysis: application to qPCR assays for Fusarium verticillioides and F. proliferatum. Anal. Bioanal. Chem. 401:717-726.   DOI
32 Plante, D., Belanger, G., Leblanc, D., Ward, P., Houde, A. and Trottier, Y. L. 2010. The use of bovine serum albumin to improve the RT-qPCR detection of foodborne viruses rinsed from vegetable surfaces. Lett. Appl. Microbiol. 52:239-244.
33 Proctor, R. H., Desjardins, A. E., McCormick, S. P., Plattner, R. D., Alexander, N. J. and Brown, D. W. 2002. Genetic analysis of the role of trichothecene and fumonisin mycotoxins in the virulence of Fusarium. Eur. J. Plant Pathol. 108:691-698.   DOI
34 Usleber, E., Straka, M. and Terplan, G. 1994. Enzyme immunoassay for fumonisin B1 applied to corn-based food. J. Agric. Food Chem. 42:1392-1396.   DOI
35 Proctor, R. H., Desjardins, A. E., Plattner, R. D. and Hohn, T. 1999. A polyketide synthase gene required for biosynthesis of fumonisin mycotoxins in Gibberella fujikuroi mating population A. Fungal. Genet. Biol. 27:100-112.   DOI
36 Srobarova, A., Moretti, A., Ferracane, R., Ritieni, A. and Logrieco, A. 2002. Toxigenic Fusarium species of Liseola section in pre-harvest maize ear rot, and associated mycotoxins in Slovakia. Eur. J. Plant Pathol. 108:299-306.   DOI
37 Tansakul, N., Limsuwan, S. and Trongvanichnam, K. 2012. Fumonisin monitoring in Thai red cargo rice by reversed-phase high-performance liquid chromatography with electrospray ionization ion trap mass spectrometry. Int. Food Res. J. 19:1561-1566.
38 Wang, E., Norred, W. P., Bacon, C. W., Riley, R. T. and Merrill, A. H. 1991. Inhibition of sphingolipid biosynthesis by fumonisins. Implications for diseases associated with Fusarium moniliforme. J. Biol. Chem. 266:14486-14490.
39 Williams, L. D. and Munkvold, G. P. 2008. Systemic infection by Fusarium verticillioides in maize plants grown under three temperature regimes. Plant Dis. 92:1695-1700.   DOI
40 Williams, L. D., Glenn, A. E., Zimeri, A. M., Bacon, C. W., Smith, M. A. and Riley, R. T. 2007. Fumonisin disruption of ceramide biosynthesis in maize roots and the effects on plant development and Fusarium verticillioides-induced seedling disease. J. Agric. Food Chem. 55:2937-2946.   DOI
41 Wei, T., Lu, G. and Clover, G. 2008. Novel approaches to mitigate primer interaction and eliminate inhibitors in multiplex PCR, demonstrated using an assay for detection of three strawberry viruses. J. Virol. Methods 151:132-139.   DOI
42 Bezuidenhout, S. C., Gelderblom, W. C. A., Gorst-Allman, C. P., Horak, R. M., Marasas, W. F. O., Spiteller, G. and Vleggaar, R. 1988. Structure elucidation of the fumonisins, mycotoxins from Fusarium moniliforme. J. Chem. Soc. Chem. Commun. 11:743-745.
43 Adejumo, T. O., Hettwer, U. and Karlovsky, P. 2007. Survey of maize from south-western Nigeria for zearalenone, alpha- and beta-zearalenols, fumonisin B1 and enniatins produced by Fusarium species. Food Add. Contam. 24:993-1000.   DOI
44 Anukul, N., Maneeboon, T., Roopkham, C., Chuaysrinule, C. and Mahakarnchanakul, W. 2014. Fumonisin and T-2 toxin production of Fusarium spp. isolated from complete feed and individual agricultural commodities used in shrimp farming. Mycotoxin Res. 30:9-16.   DOI
45 Bai, G. H. and Shaner, G. 1996. Variation in Fusarium graminearum and cultivar resistance to wheat scab. Plant Dis. 80:975-979.   DOI
46 Bhargava, S. N., Shukla, D. N., Singh, N. K. and Singh, N. 1979. Fusarium moniliforme causing panicle rot of rice. Indian Phytopathol. 31:367-369.
47 Bottalico, A., Logrieco, A., Ritieni, A., Moretti, A., Randazzo, G. and Corda, P. 1995. Beauvericin and fumonisin B1 in preharvest Fusarium moniliforme maize ear rot in Sardinia. Food Addit. Contam. 12:599-607.   DOI
48 Brown, D. W., Butchko, R. A. E., Busman, M. and Proctor, R. H. 2007. The Fusarium verticillioides FUM gene cluster encodes a Zn(II)2Cys6 protein that affect FUM gene expression and fumonisin production. Eukaryot. Cell 6:1210-1218.   DOI
49 Cankar, K., Stebih, D., Dreo, T., Zel, J. and Gruden, K. 2006. Critical points of DNA quantification by real-time PCR effects of DNA extraction method and sample matrix on quantification of genetically modified organisms. BMC. Biotech. 6:37-51.   DOI