• BMB Reports
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• v.35 no.4
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• pp.428-431
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• 2002

The often-encountered problem of disrupting bacteria for the purpose of extracting soluble protein has generated various methods. Many require specialized equipment. Very often, especially during preliminary studies, investigators need a simple, fast, and inexpensive method for cell disruption that preserves biological activity. This paper compares some simple and inexpensive methods for cell disruption, such as bead-vortexing, freesing-thawing, French pressing, and sonication. It also provides some tips to increase protein yield and preserve biological activity. If performed under optimal conditions, bead-vortexing gives protein yields that are comparable to French pressing and sonication. It also preserves the activities of labile enzymes and releases periplasmic enzymes. Vortexing with glass beads appears to be the simplest method for cell disruption.

• International Journal of Advanced Culture Technology
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• v.3 no.2
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• pp.67-76
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• 2015

This paper aims to study and design of Organic Rankine Cycle (ORC) Machine using Isopentane as working fluid expanding through Tesla turbine. The study on ORC machine expanding through Tesla turbine has result on the efficiency of Tesla turbine. In addition, Thermodynamics theory on isentropic efficiency proved to be a successful method for overcoming the difficulties associated with the determination of very low torque at very high angular speed. By using an inexpensive experiment device and a simple method, the angular acceleration method, for measuring output torque and power in a Tesla turbine is able to predict a tendency of output work. The experiments using two Tesla turbine sizes, the first size is 1.6 bigger than the second one. In comparison with the first size, the tesla turbine can produce power output more than 62% of the second size. Further study on the machine can be developed throughout the county due to its low cost and efficiency.

• BMB Reports
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• v.38 no.3
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• pp.370-372
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• 2005

A cross-linked leucaena (Leucaena leucocephala) seed gum (CLLSG) matrix was prepared for the isolation of galactose-specific lectins by affinity chromatography. The matrix was evaluated for affinity with a known galactose-specific lectin from the seeds of snake gourd (Trichosanthes anguina). The matrix preparation was simple and inexpensive when compared to commercial galactose-specific matrices (i.e. about 1.5 US$/100 ml of matrix). The current method is also useful for the demonstration of the affinity chromatography technique in laboratories. Since leucaena seeds are abundant and inexpensive, and the matrix preparation is easy, CLLSG appears to be a promising tool for the separation of galactose-specific lectins.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2004.05a
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• pp.88-92
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• 2004

Most studies in the past in testing and benchmarking on Intrusion Detection System (IDS) were conducted as comparisons, rather than evaluation, on different IDSs. This paper presents the evaluation of the performance of one of the open source IDS, snort, in an inexpensive high availability system configuration. Redundancy and fault tolerance technology are used in deploying such IDS, because of the possible attacks that can make snort exhaust resources, degrade in performance and even crash. Several test data are used in such environment and yielded different results. CPU speed, Disk usage, memory utilization and other resources of the IDS host are also monitored. Test results with the proposed system configuration environment show much better system availability and reliability, especially on security systems.

• Journal of the Korean Institute of Navigation
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• v.24 no.4
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• pp.299-312
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• 2000

Because shore(ship) to ship(shore) communication service via INMARSAT pay expensive cost, satellite service typically use two stage service that land users dial up LES(Land Earth Station) for sending or receiving message. In order to send and receive message, land users must maintain online state with LES on dedicated line. Also, two stage INMARSAT communication service must consume long time that processes step by step. In case of large company, they have own communication system, but small companys communicate simple messages with only telex. This paper describes the design and implementation of an integrated communication services via INMARSAT-C. This system uses inexpensive INMARSAT-C for transmission between ship and land. Because this system provides web and E-Mail interface, users send and receive messages easily and quickly. This system provides most users with inexpensive and easy communication facilities between ship and land.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 1999.04a
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• pp.140-141
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• 1999

• Journal of Electrochemical Science and Technology
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• v.7 no.3
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• pp.190-198
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• 2016

Application of fuel cell to produce renewable energy for commercial purpose is limited by the high cost of Pt based electrode materials. Development of inexpensive, high energetic electrode is the need of the hour to produce pollution free energy using bio-fuel through a fuel cell. Ni-Cu and Ni-CeO₂-Cu electrode materials, electro synthesized by pulse current have been developed. The surface morphology of the electrode materials is controlled by different deposition parameters in order to produce a high current from the electro-oxidation of the fuel, the ethanol. The developed materials are electrochemically characterized by Cyclic Voltammetry (CV), Chronoamperometry (CA) and Potentiodynamic polarization tests. The results confirm that the high current is due to their enhanced catalytic properties viz. high exchange current density (i₀), low polarization resistance (R_p) and low impedance. It is worthwhile to mention here that the addition of CeO₂ to Ni-Cu has outperformed Pt as far as the high electro catalytic properties are concerned; the exchange current density is about eight times higher than the same on Pt surface. The morphology of the electrode surface examined by SEM and FESEM exhibits that the grains are narrow and sub spherical with 3D surface, containing vacancies in between the elongated grains. The fact has enhanced more surface area for electro oxidation of the fuel, giving rise to an increase in current. Presence of Ni, CeO₂, and Cu is confirmed by the XRD and EDXS. Fuel cell fabricated with Ni-CeO₂-Cu material electrode is expected to produce clean electrical energy at cheaper rates than conventional one, using bio fuel the derived from biomass.

• Journal of IKEEE
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• v.23 no.1
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• pp.295-301
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• 2019

A heater in a portable real-time polymerase chain reaction(PCR) system is one of the important factors for controlling the PCR thermocycle precisely. Since heaters are integrated on a small-sized PCR chip for rapid heating and fabricated by semiconductor processes, the cost of producing PCR chips is high. Here, we propose to use chip resistors as an inexpensive and accurate temperature control method. The temperature distribution was simulated using one or two chip resistors on a real-time PCR chip and the PCR chip with uniform temperature distribution was fabricated. The temperature rise and fall rates were $18^{\circ}C/s$ and $3^{\circ}C/s$, respectively.

• Bulletin of the Korean Chemical Society
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• v.24 no.7
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• pp.885-886
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• 2003

• Journal of Plant Biotechnology
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• v.37 no.3
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• pp.243-249
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• 2010

Molecular farming is production of pharmaceutically and industrially important proteins in plants. Plants and plant cell culture systems have been used as bio-factory to produce recombinant proteins such as monoclonal antibodies, enzymes, vaccines, hormones, interleukins, commercial enzymes and etc. The terms molecular farming, biofarming, molecular pharming, phytomanufacturing, recombinant or plant-made industrials, planta-pharma, plant bioreactors, plant biofactory, and pharmaceutical gardening are used interchangeably. Molecular farming can provide safe and inexpensive pharmaceutical proteins as well as commercial ones. In spite of several advantages of molecular farming such as safety and inexpensive cost, there are also a couple of drawbacks in the existing technology. One of them is low expression level of target gene in plants, which has been improved by optimizing gene-based codon usage, screening of strong promoters, expression of transcription factors, subcellular targeting of target proteins, chloroplast transformation, and transient expression using viral expression system (magnifection). Some plant-based commercial proteins have already been in markets and more than twenty plant-based pharmaceuticals have been in clinical trials, from that we can expect that several plant-based pharmaceutical proteins will be seen in the markets in the near future.