• International Journal of Industrial Entomology and Biomaterials
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• 제17권2호
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• pp.205-209
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• 2008

Impact of food plant on reproductive and commercial parameters in Antheraea mylitta, a polyphagous insect of economic importance was studied upon feeding the insect larvae on the same host plants for six continuous generations. A. mylitta larvae were fed upon Terminalia tomentosa, Terminalia arjuna and Zizyphus jujuba and restricted them to the same host plant for six generations to document the quantitative improvement in reproductive and commercial parameters. The parameters showed significant improvement in all the host plants studied over their respective controls. Fecundity among the reproductive parameters was highly improved than others (85.9% in T. tomentosa; 58% in T. arjuna and 49.7% in Z. jujuba). Likewise in commercial parameters, the shell weight in male showed the highest improvement (by 52.9%, 45.8% and 42.1% in T. tomentosa; T. arjuna and Z. jujuba respectively). On the other hand, the shell ratio percentage in female recorded the lowest improvement. The values for all characters were recorded a decline in T. arjuna and Z. jujuba fed ones over T. tomentosa, except that of shell ratio percentage in female has registered an increase in Z jujuba fed. The study thus revealed the comparative superiority of T. tomentosa over T. arjuna and Z. jujuba.

• International Journal of Industrial Entomology and Biomaterials
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• 제23권1호
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• pp.137-141
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• 2011

A previous data was provided information for tissuespecific expression genes by means of whole-genome oligonucleotide microarray in the silkworm. We analyzed the tissue-specific expression patterns in the hemocyte tissue on 5 days of 5th instar larvae during the development of $B.$ $mori$. Total 5 candidates pick out from the $Bombyx$ $mori$ Microarray Database (BmMDB; http://silkworm.swu.edu.cn/microarray). To verify the hemocyte-specific expression, we analyzed by semi-quantitative and real-time quantitative RT-PCR using the highly expressed endogenous $Actin$ RNA as an intrinsic reference. In this study, we confirmed that one gene-sw17255- out of 5 candidates expressed in the hemocyte tissue, which was consistent with the previous data. Circulating hemocytes in the body fluid of the $B.$ $mori$ are most powerful target organ for producing biomaterials. We need further studies to find hemocyte-specific promoter region from sw17255 gene. Finally, this result can be applied in creating transgenic silkworms as a biomedical insect.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권2호
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• pp.235-239
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• 2003

The silkworm Bombyx mori L. was studied as the potential “host” of popular in Uzbekistan biocontrol ectoparasite, entomophagous Bracon hebetor Say. Being one of representatives of economic-beneficial insects, the silkworm (larvae, pupae and imago) can be used as highly sensitive test organism for revealing of neuro toxic effects of insects venom as well as of their purified components in screening assays. In comparative aspect, except a mulberry silkworm, representatives of Uzbekistan pests cotton-boll worm Helycoverpa armigera Hbn., lesser mulberry pyralid Glyphodes pyloalis Wlk., codling moth Corpocapsa pomonella L., malaria mosquito Anopheles claviger and Colorado potato beetle Leptinotarsa decemlineata Say have been subjected to insect toxic test of bracon venom gland extract (VGE) and its fractions which were obtained by gel-chromatography on Sephadex G-100. The paralyzing effect of the VGE and its fractions was shown in a various degree on the pests.

• International Journal of Industrial Entomology and Biomaterials
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• 제22권2호
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• pp.51-57
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• 2011

Reproductive activity in the economically important insect, Antheraea mylitta is very important because the female reproduces only once in her life time and the aging is very rapid and costly. It is a capital breeder and strictly a monoandrous species. To know how strategically the insect behaves and the male allocates its ejaculate resource more prudently during its successive mating with virgin females to maximize its own fitness and the fitness of the female with whom it mated, both being most important to sericulture industry. So, the present study was undertaken and the results revealed fresh and virgin females always prefer to mate with fresh virgin males (84%) and receives high dose of ejaculates leading to higher hatchability than to virgin males of one day old (13.7%) and one day old males with mating experience (2.3%). The ejaculation size (as referred to eupyrene sperm count in the ejaculation) declined significantly over successive mating (r = -0.9931, P < 0.001), so also the male body weight (r = -0.9560, P < 0.001). The quantity of ejaculate passed to female also dramatically declined during aging (r = -0.9982, P < 0.001). It was found that male weight contribute substantially to the quantum of ejaculate (r = -0.9519, P < 0.001), so also higher fecund females receive relatively more ejaculate than the lower group to reach higher reproductive fitness. The life time fecundity was found to be $334{\pm}31$.

• International Journal of Industrial Entomology and Biomaterials
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• 제35권2호
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• pp.118-122
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• 2017

Porcine pseudorabies virus (PRV) causes the Aujeszky's disease (AD) which is economically important disease in the swine industry worldwide. Killed or live vaccines have been used to control this disease, but their efficacy and side effects remain problems to be solved. To solve these problems, in this study, production of recombinant PRV glycoprotein gB, gC and gD was investigated in insect expression system. Glycoprotein gB, gC and gD are regarded as the major immunogenic antigens in PRV. Abundant production and immunogenicity of glycoprotein gB, gC and gD were confirmed by SDS-PAGE and Western blot analysis, respectively. Optimal infection dose and time were also determined for the production of each recombinant PRV glycoprotein. Confirmation of glycosylation of recombinant gB, gC and gD suggested their usefulness as antigens for the development of diagnosis kit or vaccines for Aujeszky's disease.

• International Journal of Industrial Entomology and Biomaterials
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• 제45권2호
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• pp.108-114
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• 2022

Protaetia brevitarsis seulensis larva is well-known as an edible insect. The present study aimed to explore the immune-enhancing effect of 30% ethanol extract of Protaetia brevitarsis seulensis larvae (PBE) in RAW264.7 macrophage cells. PBE were not cytotoxic to RAW264.7 cells and nitric oxide production increased on PBE treatment in a dose-dependent manner. Furthermore, PBE significantly promoted the expression of immune-related mediators (Inos and COX-2) and cytokines (TNF-α, IL-6, and IL-1β) and the phosphorylation of MAPKs (ERK, p38, and JNK). Taken together, the immune-enhancing effects of 30% ethanol extract of PBE in vitro were identified. These findings can be used as data for the development of edible insect-based functional foods that improve immune function.

• International Journal of Industrial Entomology and Biomaterials
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• 제48권2호
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• pp.86-97
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• 2024

Alkaline- or salt-assisted extractions have been widely used to extract edible insect proteins, however, there is a need for extraction techniques that balance cost-efficient production as well as preserving the protein properties. Mealworm proteins (Tenebrio molitor larvae) were extracted using three different extraction methods-alkali (AMP), salt (SMP), and water (WMP)-and then physicochemical and techno-functional properties were examined. AMP had high yield, protein, and amino acid contents, whereas WMP had high moisture, ash, and fat contents. SDS-PAGE showed a wide range of molecular weights in WMP whereas mostly low molecular weights were observed in AMP and SMP. AMP had poor protein solubilities compared to SMP and WMP across all pHs. AMP had enhanced water-holding capacity and emulsion stability, whereas WMP had improved oil-holding capacity and foaming properties. WMP formed a gel with and without the transglutaminase. The physicochemical and techno-functional properties demonstrated that water-soluble mealworm protein was superior to alkali-and salt-soluble mealworm proteins. Considering the cost efficiency and minimal impact on the environment as well, a cold press juicer could be utilized for mass production of mealworm protein compared to the conventional methods of protein extraction using alkali and salt.

• International Journal of Industrial Entomology and Biomaterials
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• 제48권2호
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• pp.67-77
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• 2024

This study aims to assess the efficacies of exogenous enzymes, derived from invertebrate gut-associated microbes, as feed additives, in reducing volatile organic compound (VOC) emissions using an in vitro pig intestine continuous fermentation system. An in vitro continuous fermentation model was used to simulate a comparable bionic digestion system by co-reacting feed, enzymatic additives (arazyme, mannanase, and xylanase, derived from the gut bacteria of Nephila clavata, Eisenia fetida, and Moechotypa diphysis, respectively), and gastrointestinal microbes, followed by an analysis of their correlations. A significant correlation was observed between exogenous enzyme supplementation and reduced VOC emissions in the fecal phase of continuous fermentation (p < 0.05). The concentration of VOCs decreased by 3.75 and 2.75 ppm in the treatment group following arazyme and multi-enzyme supplementation, respectively, compared to that in the control group (7.83 ppm). In addition, supplementation with arazyme and multiple enzymes significantly affected the microbial composition of each fermentation phase (p < 0.05). In particular, Lactiplantibacillus pentosus and Pediococcus pentosaceus, which changed in abundance according to arazyme or multi-enzyme supplementation, exhibited a positive relationship with VOC emissions. These results suggest that exogenous enzymes derived from invertebrate gut-associated bacteria can be efficiently applied as feed additives, leading to a reduction in VOC emissions.

• International Journal of Industrial Entomology and Biomaterials
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• 제43권1호
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• pp.22-28
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• 2021

Natural nonwoven silk fabric has attracted researchers' attention owing to its unique properties as a biomaterial. It is fabricated by reeling, wetting, and hot pressing natural silk filament from silkworm cocoons. In this study, silkworm cocoons were immersed in water at different temperatures for various durations to examine the effects of wet treatment on their crystallinity and morphology. As the treatment temperature and time increased, the cocoon's silk filament separation became more evident, and fibroin strands were observed in it. The crystallinity indexes of the silkworm cocoons increased until a treatment time of 2 h and remained constant thereafter. The increase in the crystallinity index using wet treatment was enhanced by increasing the treatment temperature. Although the weight loss of the silkworm cocoon was insignificant until 40℃, it was appreciable above this temperature, and the degree of weight loss constantly increased with increases in the treatment temperature and time.

• International Journal of Industrial Entomology and Biomaterials
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• 제42권1호
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• pp.14-20
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• 2021

Mulberry was dried with low temperature vacuum dryer using microwave. The nutritional compositions of microwave-dried mulberry including proximate composition, sugar content, mineral content, total phenolic, flavonoids, and anthocyanin, beta-carotene, vitamin C, and amino acid composition were measured. Sugar contents of mulberry were 42.6 mg/100g (Cheongilppong) and 43.27 mg/100g dw (Gwasang No. 2). The main components of mulberry sugars were fructose and glucose. Mineral analysis showed that K, P, Ca, and Mg were abundant regardless of mulberry cultivars.