• Korean Journal of Food Science and Technology
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• v.37 no.6
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• pp.861-865
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• 2005

2,4-Dinitrophenylhydrazine (DHP), indophenol, and HPLC methods, which are generally used to analyze vitamin C, were evaluated by comparing changes in vitamin C contents in various samples kept at 10 and $20^{\circ}C$. Amount of total ascorbic acid (TAA) in yoghurt A kept at $10^{\circ}C$, as measured by DNP method, decreased from 26.7mg/100mL for early period to 22.8mg/100mL for final period of storage, whereas, on 3rd and 10th storage days, increased contrary to general results. Using indophenol, TAA measured at 28.6 and 9.5mg/100mL, and 30.5 and 14.6mg/100mL using HPLC far early and final periods, respectively. With indophenol and HPLC, TAA amount showed steady tendency to decrease. In conclusion, HPLC is the most suitable method to analyze vitamin C content, and indophenol method can be also used during early storage stage.

• Journal of Environmental Science International
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• v.4 no.1
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• pp.91-103
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• 1995

The reaction rate, equilibrium, and flow injection analysis methods were fundamentally evaluated for the determination of aqueous ammonia. The selected indophenol blue method was based on the formation of indophenol blue in which ammonium ion reacted with hypochlorite and phenol in alkaline solution. In the optimized reaction condition, the reaction followed 1st order reaction kinetics and the final product was stable. The absorbance measurements before and after the equilibrium were utilized for the reaction rate and equilibrium methods. The reaction rate methods, based on the relative analytical signals for the possibility of eliminating interferents, were shown to have good linear calibration curves but the detection limit and the calibration sensitivity were poorer than those in the equilibrium method. The detection limits were 32-49 pub and 24 pub for the reaction rate and equilibrium methods, respectively In the flow injection analysis, the absorbance was measured before the equilibrium reached and thus resulted in 30% reduction of calibration sensitivity. However, the detection limit was 11 ppb, indicating that the peak-to-peak noise for the blank was remarkably improved. Compared to the manual methods, the optimized experimental condition in a closed reaction system reduced the blank absorbance and the inclusion of ammonia from the atmosphere was prevented. In addition, highly reproducible mixing of sample and reagents and analytical data extracted from continuous recording showed excellent reproducibility.

• Bulletin of the Korean Chemical Society
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• v.30 no.9
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• pp.2032-2038
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• 2009

The amount of phenol and NaOH for the colorimetric determination of ammonia in Korean standard methods (KSM) is found to be highly excessive compared to the standard methods of several other countries. The absorbance of indophenol formed by the Berthelot reaction for ammonia analysis was measured under the various reaction conditions classified in experiment groups 1, 2, 3, 4 and KSM and American standards methods (ASM), and the relationships between the absorbance of indophenol and concentration of ammonia were compared. The amount of phenol can be reduced to 10 g (current 25 g in KSM) and NaOH can be reduced to 1.76 g (current 11 g in KSM) for the preparation of 200 mL phenate solution, and the absorbance sensitivity increased. The concentration of the phenol and NaOH correlatively affect the pH of the solution, which is a critical variable in achieving the maximum sensitivity and rapid and stable color development.

• Korean Journal of Soil Science and Fertilizer
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• v.50 no.2
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• pp.100-105
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• 2017

Various methods for assessing soil total nitrogen (TN) and inorganic N content have been developed to manage nutrient and to understand N cycle in soil. This paper address the technical procedures in arable soil samples to conduct soil sampling, sample preparation, and measuring total N and inorganic N. Among various methods for measuring soil total nitrogen contents, Kjeldahl distillation and Indophenol blue method have widely used due to reliability and economic advances. Also, two methods can analyze more samples at the same time compared with other nitrogen measuring methods. For evaluating inorganic N content, mainly in forms of nitrate-N ($NO_3{^-}-N$) and ammonium-N ($NH_4{^+}-N$), extraction with a single reagent such as 2M KCl has been employed, followed by Kjeldahl distillation or indophenol blue methods.

• Journal of Korean Society for Atmospheric Environment
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• v.22 no.E1
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• pp.1-8
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• 2006

The purpose of this study was to evaluate the efficiency of a passive sampler in measuring atmospheric ammonia concentrations using chamber system. The ability of the passive sampler to quantitatively determine atmospheric ammonia gas was almost identical to that of the reference method (indophenol method). There was no significant difference between concentrations measured by the two methods. The detection and quantification limits of the ammonia passive sampler were 16.9 ppb and 25.3 ppb, respectively, for a 24-h sampling period. The average coefficient of variation between replicated samplers was $6.7{\pm}4.2%$. The concentrations measured by the two methods (passive sampler and indophenol method) were no significant difference with good a correlation (correlation coefficient=0.964).

• Applied Biological Chemistry
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• v.23 no.3
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• pp.173-177
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• 1980

The anti-oxidant components in Panax ginseng, which were known to have antiaging effect, were fractionated by various solvents and then isolated by the preparative thin layer chromatography. A developing system with silicagel G using toluene-chloroform-actene (20 : 13 : 13) as mobile phase was applied. The components were investigated the anti-oxidant activity by ${\alpha}$, ${\alpha}'-diphenyl-{\beta}-picrylhydrazyl$ (DPPH) at 517nm. Two effective components were obtained from red ginseng and were indophenol reducing substances.

• Journal of Korean Society of Environmental Engineers
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• v.38 no.12
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• pp.667-675
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• 2016

Nanoscale zero-valent iron (nZVI) has been effectively applied for environmental remediation due to its ability to reduce various toxic compounds. However, quantification of nZVI reactivity has not yet been standardized. Here, we adapted colorimetric assays for determining reductive activity of nZVIs. A modified indophenol method was suggested to determine reducing activity of nZVI. The method was originally developed to determine aqueous ammonia concentration, but it was further modified to quantify phenol and aniline. The assay focused on analysis of reduction products rather than its mother compounds, which gave more accurate quantification of reductive activity. The suggested color assay showed superior selectivity toward reduction products, phenol or aniline, in the presence of mother compounds, 4-chlorophenol or nitrobenzene. Reaction conditions, such as reagent concentration and reaction time, were optimized to maximize sensitivity. Additionally, pretreatment step using $Na_2CO_3$ was suggested to eliminate the interference of residual iron ions. Monometallic nZVI and bimetallic Ni/Fe were investigated with the reaction. The substrates showed graduated reactivity, and thus, reduction potency and kinetics of different materials and reaction mechanism was distinguished. The colorimetric assay based on modified indophenol reaction can be promises to be a useful and simple tool in various nZVI related research topics.

• Journal of the Korean Home Economics Association
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• v.4 no.1
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• pp.589-594
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• 1963

Ascorbic acid has a strong power to reduce other materials, so by using this reducing power we can analise many materials quantitatively. Dihydro-ascorbic acid is quantitatively changed into ascorbic acid under a certain condition, if reduced by hydrogen-sulfide. Dihydro-ascorbic acid also has physiolosical effect, but less effect than ascorbic acid. The effect of dihydro-ascorbic acid is regarded as a half of that of ascorbic acid. Among the analytical methods applying this theory the method using 2.6 dichlorophenol indophenol what called the method of Indophenol is used most widely; so does Hydrazine-method. But comparing these two methods the former shows a little higher rate in analytical value. Vegetable are vital sources for vitamin C. According to the report of the commitee of FAO Korean branch the amount of daily per head average intake of vitamin C is about 70mg in raw materials. Since vitamin C is easily affected by heating or oxidation in Cooking the loss is not a little. Consequntly it is regarded that the actual amount of intake will be much less than basic amount. It is therefore very important to find out that how much percent of the loss there will be in case of cooking, in order to dicide the actual amount of in take as proper nutrition for a person. Therefore this paper intended to give some help in setting a standard amount of V.C intake, by measuring the change of the V.C amount using the general cooking method and by measuring amount of V.C contained in the part of vegetable, used in Korea abundantly.

• Journal of Sensor Science and Technology
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• v.2 no.1
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• pp.41-48
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• 1993

An ISFET urea sensor was fabricated by immobilizing the urease using polyurethane on the $H^{+}$ sensing $Si_{3}N_{4}$ thin film of pH-ISFET. The sensor could determine the urea concentration $1{\sim}50$ mg/dl with fast response of $3{\sim}5$ min. and good repeatability. For its application to clinical analysis, the results of the urea measurements in blood plasma using ISFET urea sensor were compared with these of conventional Urease-Indophenol method.

• Korean Journal of Food Science and Technology
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• v.37 no.3
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• pp.352-359
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• 2005

Uncertainty involved during determination of vitamin C content in infant formula was quantified by indophenol titration method. Uncertainty sources in measurand, such as purity, weight, final volume of standard, volume of standard solution used for titration, sample weight, final volume of sample, extraction solution used for titration, titration of extraction solution and standard solution by indophenol solution were identified and used as parameters for combined standard uncertainty based on Guide to the expression of uncertainty in measurement (GUM) and Draft EURACHEM/CITAC Guide. Uncertainty parameters of each source in measurand were identified as resolution, reproducibility and stability of chemical balance, standard material purity, repeatability, reproducibility, end point of titration, 1 mL pipet, 5 mL autopipet, and 100 mL mass flask. Each uncertainty component was evaluated by types A and B and included to calculate combined uncertainty. Analytical test result for traceability under laboratorial conditions using Certified Reference Material (CRM) test was certified as $108.4{\pm}1.7mg/100g$, which was within CRM certification range of $114.6{\pm}6.6mg/100g$. Uncertainty test result of vitamin C content of 5 g sampling was $56.7{\pm}2.44mg/100g$. Uncertainty could be reduced by identification of uncertainty sources and components related with vitamin C determination by indophenol titration method and by decreasing uncertainty sources and components.