• Proceedings of KHEA Conference
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• 1997.07a
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• pp.186-186
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• 1997

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.901-901
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• 2003

• Microbiology and Biotechnology Letters
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• v.47 no.3
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• pp.449-458
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• 2019

Hepatitis B treatments using immune therapy are gaining interest because of the improvements in dendritic cell performance for antigen presentation, which induces an appropriate immune response and raises patient survival rates. This research aims to produce a significant amount of the HBcAg antigen, which can induce an immune response and have a curative effect on HBV infection. In this study, the HBV subgenotype B3 of the HBcAg gene was used, which is dominant in Indonesia. Further, Lactococcus lactis bacteria was used as the host because of its safety and tightly regulated protein expression. The codon usage for the HBcAg gene was optimized to improve protein expression in L. lactis, which is important because a codon is not random between species. The HBcAg gene is attached to a pNZ8148 plasmid and transformed into the L. lactis NZ3900 expression host. The results confirm that a positive protein band (21 kDa) in two fractions of purified HBcAg was recognized by both western blotting and dot blot hybridization, even if the HBcAg optimized codon has higher GC contents than that suggested for L. lactis expression. Overall, this research strengthens the broad use of L. lactis bacteria for any protein expression, including higher protein expression of codon optimized HBcAg gene compared to non-optimized genes. Furthermore, the improvement in the codon optimization of the HBcAg gene significantly increases the total protein expression by 10-20%, and the expression level of the codon optimized HBcAg increases 1.5 to 3.2-times that of the native HBcAg.

• Mycobiology
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• v.31 no.1
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• pp.19-22
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• 2003

The primary objective of the current research was to detect genetic variations within the Indonesian isolates of Cochliobolus heterostrophus collected from ecologically different places of the country at molecular level using PCR-RFLP analyses. The primer pair of NS3 and NS6 produced amplification fragment in all of the isolates tested. A single fragment of estimated 907 bp was observed in the PCR product pattern. RFLP analysis of the PCR product employing three restriction enzymes, HaeIII, HhaI, and RsaI, respectively, did not reveal intraspecific variations within the fungus. Similarly, nucleotide sequences of portion of small subunit of the ribosomal DNA gene of two of the isolates collected showed no appreciable differences, indicating the absence of genetic diversities among the isolates tested. A phylogenetic tree was constructed and the Indonesian C. heterostrophus, represented by SM-1 isolate, was found to be phylogenetically located near C. sativus, a closely related species.

• The Korean Journal of Ecology
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• v.23 no.2
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• pp.125-129
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• 2000

Gunung Gede Pangrango National Park is one of the Long-term Ecological Research Site in Indonesia. In the late 1997, the fires have burnt and destroyed nearly 300 ha forest in this park. and G. Masigit was the largest burnt area (250 ha) of nine locations of hot spot recognized. Undergrowth vegetation got the most severe impacts. Almost undergrowth vegetation in various location were totally burnt. However, within three months following burning new seedlings such as Omalanthus populneus, Macaranga, Trema orientalis and Eupatorium appeared in the forest floor- The number of mycoflora recorded in burnt forest was interestingly increased in post forest fires site. Forest fires in G. Masigit had also affected the wild life population and diversity. For example, the number of bird species and the number of soil insects in burnt forest was significantly reduced. The forest fires had also great impact on soil. such as on soil organic contents, bulk density, colour, consistency, permeability and the activity of soil microorganisms.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.1
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• pp.27-35
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• 1993

In an attempt to reveal the interrelationship between fowls of jungle and native origin, their gene constitutions were compared using gene frequencies at the 16 loci controlling blood protein variations. Of the 16 loci analysed by electrophoresis, polymorphism was detected at following seven loci: Es-1, Amy-1, Akp-akp, Akp-2, Alb, Tf and 6-PGD. The other nine loci: Amy-3, Es-D, PGM, PHI, MDH, To, LDH, Hb-1 and Hb-2, were noted to be monomorphic. Genetic distance between pairs of native fowl and jungle fowls was estimated by a numerical taxonomic method. The Indonesian native fowl was genetically close to the Indonesian red jungle fowl, and the grey jungle fowl was genetically similar to the Ceylonese jungle fowl. It was also suggested that the green jungle fowl was genetically remote from the other jungle fowls and from the Indonesian native flow. The proportion of polymorphic loci (Ppoly), the expected average heterozygosity per individual $\bar{H}$, and the effective number of alleles per locus (Ne) were calculated to evaluate the genetic variabilities in the native and jungle fowls. The Indonesian native fowl exhibited slightly higher the proportion of polymorphic loci than the jungle fowls.

• Asian Journal of Innovation and Policy
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• v.9 no.3
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• pp.281-307
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• 2020

A variety of approaches are being applied to improve the existing ex-ante evaluation by expert panels in publicly funded R&D. While the objective evaluation criteria are constantly being improved to screen and select the superior projects, alternative approaches such as random prioritization and logical modeling are also underway to overcome the conservative bias of reviewers and to secure disruptive innovation. This study intends to find critical implications for ex-ante evaluation of public R&D system from the comparison of Indonesia and Korea. For the comparative analysis, literature review and expert in-depth interviews are conducted on the national R&D system and the selection evaluation process. In Korea, the selection criteria of projects are legally promulgated for establishing an objective evaluation system, and at the program level, the major considerations in the planning process are specified by Presidential Decree. On the other hand, while Indonesia conducts R&D in 47 strategic fields largely by public research institutes (PRI) based on the non-competitive government contributions. This study draws out implications of institutionalizing the planning process at the level of program, and of increasing the ratio of contract-based competitive funding at the level of project in the national R&D portfolio.

• The Southeast Asian review
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• v.23 no.3
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• pp.181-215
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• 2013

The purpose of this paper is to examine the process of Islamic change in Indonesia since the 1970s, which is commonly called 'Islamic resurgence' or 'Islamization.' A brief analysis of the reasons for Islamic resurgence is followed by discussions on its long-term effects on the life of Indonesian Muslims. Shift in the ways Islamic matters have been treated in public, gradual realization of what Islamic groups have demanded in non-religious areas, stipulation of local regulation based on shariah and spread of radicalism are to be investigated one by one. With these examinations, it will be argued that the most significant change throughout the last four decades has been the surge of Islam as a reference point to interpret everyday life of Muslims. The dichotomy of 'Islamic' and 'non-Islamic' is instated as a key in Islamic discourse and the Quran and Hadith, as a criterion to judge whether certain views and behavior are Islamic or not. These have strengthened the position of scripturalism in Indonesian Islam. Islamic resurgence has also resulted in the acceleration of the diversification of Indonesian Muslims. Muslims with radical, fundamental, reformist, traditional and liberal views coexist, competing for stronger social and religious influences. As radical and fundamental groups have been more active in dealing with socio-political affairs recently, whether their active involvement will result in wider influences over the ordinary Muslims is the key to understand the future dynamics of Indonesian Islam.

• The Journal of Asian Finance, Economics and Business
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• v.9 no.9
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• pp.351-360
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• 2022

This study aims to determine the factors that can influence investors in selecting stocks in the Indonesian capital market to establish an optimal portfolio, and find phenomena that occurred during the COVID-19 pandemic so that buying interest / the number of investors increased in the Indonesian capital market. This study collection technique uses primary data obtained from the survey questionnaire and secondary data which is market data, stock price movement data sourced from the Indonesia Stock Exchange, Indonesian Central Securities Depository, and Bank Indonesia, as well as empirical literature on behavior finance, investment decision, and interest in buying stock. The method used in this research is the survey questionnaire analysis with the SEM (statistical approach). The results of the analysis using SEM show that investor behavior influences the stock-buying interest, investor behavior, and the stock-buying interest influences investor decision-making. However, risk management does not influence investor-decision making. This occurs when the investigator's psychological capacity produces more decision information by decreasing all potential biases, allowing the best stock selection model to be selected. When the investigator's psychological capacity creates more decision information by reducing biases, the optimum stock selection model can be chosen.

• Journal of Veterinary Science
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• v.23 no.2
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• pp.24.1-24.10
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• 2022

Background: Small interfering RNA technology has been considered a prospective alternative antiviral treatment using gene silencing against influenza viruses with high mutations rates. On the other hand, there are no reports on its effectiveness against the highly pathogenic avian influenza H5N1 virus isolated from Indonesia. Objectives: The main objective of this study was to improve the siRNA design based on the nucleoprotein gene (siRNA-NP) for the Indonesian H5N1 virus. Methods: The effectiveness of these siRNA-NPs (NP672, NP1433, and NP1469) was analyzed in vitro in Marbin-Darby canine kidney cells. Results: The siRNA-NP672 caused the largest decrease in viral production and gene expression at 24, 48, and 72 h post-infection compared to the other siRNA-NPs. Moreover, three serial passages of the H5N1 virus in the presence of siRNA-NP672 did not induce any mutations within the nucleoprotein gene. Conclusions: These findings suggest that siRNA-NP672 can provide better protection against the Indonesian strain of the H5N1 virus.