• Advances in nano research
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• 제9권1호
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• pp.15-24
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• 2020

Iron has a crucial role in growth as part of metalo-proteins like haemoglobin or myoglobin, enzymes; they are also involved in energetic reactions. Iron plays a vital role in fertility. At high doses, Iron has a harmful consequence on the reproductive system, which can be strongly reflected the final stage of spermatogenesis. Nutritional products are claiming to use nanotechnology and it is important to recognize the potential toxicity of nano-sized nutrients. Recently iron nanoparticles were proposed as a food additive for poultry. The objective of this study was to investigate the effects of L-cystein coated iron oxide nanoparticles on reproductive performance in male quails. The results of Fourier Transform Infrared Spectrometer, Alternating Gradient Force Magnetometer and Scaning Electron Microscopy showed that iron oxide nanoparticles was produced and have been coated with L-cycstein (Fe₃O₄-Cys NPs). A total of 100 one-week-old quail chicks were randomly placed to five groups of five replicates. Four quails (two male and two females) were raised in an individual cage for each replicate. The five experimental treatment diets consisted; negative control diet, with no Iron supplementation; positive control diet supplemented with 60 mg/kg of Fe₃O₄; treatment diets supplemented with 0.6, 6 and 60 mg/kg of L-cystein coated iron oxide nanoparticles. The hemoglobin, Red blood cell, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, gonadal somatic index, daily sperm production, total testicular sperm and sperm viability of the male quails that were fed with diet supplemented by 0.6 mg/kg of Fe₃O₄-Cys NPs were improved as compare with negative control. This study showed that not only the use of the Fe₃O₄-Cys nanoparticles had no side effects but also it can be used as a feed additive to improve the reproductive performance in male quails.

• 천문학회지
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• 제38권2호
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• pp.219-222
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• 2005

We present the high-resolution (2"-4") images of the molecular envelopes surrounding the evolved stars, V Hya, VY CMa, and ${\pi}^1$ Gru observed with the Submillimeter Array. The CO J=2-1 and 3-2 images of the carbon star V Hya show that the circumstellar structure of this star consists of three kinematic components; there is a flattened disk-like envelope that is expanding with a velocity of ${\~}16 km\;s^{-1}$, the second component is the medium-velocity wind having a deprojected velocity of 40-120 km $s^{-l}$ moving along the disk plane, and the third one is the bipolar molecular jet having an extreme velocity of 70-185 km $s^{-l}$. The axis of this high velocity jet is perpendicular to the plane of the disk-like envelope. We found that the circumstellar structure of the S-star ${\pi}^1$ Gru traced by the CO J =2-1 resembles that of V Hya quite closely; the star is surrounded by the expanding disk-like envelope and is driving the medium-velocity wind along the disk plane. We also obtained the excellent images of VY CMa with the CO and $^{13}CO$ J=2-1 and $SO\;6_5-5_4$ lines. The maps of three molecular lines show that the envelope has a significant velocity gradient in the east-west direction, suggesting that the envelope surrounding VY CMa is also flattened and expanding along its radial direction. The high-resolution images obtained with the SMA show that some AGB stars are associated with the asymmetric mass loss including the equatorial wind and bipolar jet.

• Applied Microscopy
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• 제20권2호
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• pp.57-70
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• 1990

Characterization and electron microscopic visualization of the plasmid and the gene expression of Escherichia coli were carried out. Transcriptional units of active structural genes were observed after lysis of Escherichia coli cells. The ribosomes attached to the E. coli genome on mRNA molecule as polyribosomes. From this gradient of polyribosome length, we estimated location of mRNA synthesis initiation site. In this experiment, a granule is ofen present which may correspond to a RNA polymerase at the promoter site. pOX1, pOX7, pOX7A, $pOX7{\Delta}1$, pSTP36, pSTP21, pBR322, and pJH12 were visualized by way of electron microscope, and their estimated sizes were determined to be $5.70{\pm}0.08{\mu}m,\;2.15{\pm}0.10{\mu}m,\;2.14{\pm}0.12{\mu}m,\;7.39{\pm}0.08{\mu}m,\;4.03{\pm}0.04{\mu}m,\;1.50{\pm}0.03{\mu}m\;and\;1.25{\pm}0.09{\mu}m$ respectively. One micrometer of measured length corresponded to about 3.0 Kb. Mica-press adsorption method that allows selectivs visualization of the plasmid DNA released in situ from the bacterial cell is rapid and useful for visualization of plasmids. The released plasmid DNA was adsorbed preferently on mica in a divalent cation-free solution. Miller chromatin-spreading method was useful to observe the plasmid and transcripts. BAC method and cytochrome C monolayer were useful to observe the plasmid DNA. Our ability to visualize ultrastructural aspects of the expression of E. coli has given us a unique tool with which to study the regulation the level of an individual gene.

• 천문학회지
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• 제38권4호
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• pp.429-435
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• 2005

We present BV CCD photometry for the open clusters Czernik 24 and Czernik 27. These clusters have never been studied before, and we provide, for the first time, the cluster parameters; reddening, distance, metallicity and age. Czernik 24 is an old open cluster with age $1.8{\pm}0.2Gyr$, metallicity $[Fe/H]=-0.41{\pm}0.15dex$, distance modulus $(m-M)_0=13.1{\pm}0.3mag$ ($d=4.1{\pm}0.5kpc$), and reddening $E(B-V)=0.54{\pm}0.12mag$. The parameters for Czernik 27 are estimated to be $age=0.63{\pm}0.07Gyr$, $[Fe/H]=-0.02{\pm}0.10dex$, $(m-M)_0=13.8{\pm}0.2mag$ ($d=5.8{\pm}0.5kpc$), and $E(B-V)=0.15{\pm}0.05mag$. The metallicity and distance values for Czernik 24 are consistent with the relation between the metallicity and the Galactocentric distance of other old open clusters. We find the metallicity gradient of 51 old open clusters including Czernik 24 to be ${\Delta}[Fe/H]/{\Delta}R_{gc}=-0.064 {\pm}0.009dex\;kpc^{-1}$.

• Investigative Magnetic Resonance Imaging
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• 제19권3호
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• pp.168-177
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• 2015

Purpose: Brain surface intensity model (BSIM)-based cortical thickness analysis does not require complicated 3D segmentation of brain gray/white matters. Instead, this technique uses the local intensity profile to compute cortical thickness. The aim of the present study was to evaluate intra-rater and inter-rater reliability of BSIM-based cortical thickness analysis using images from elderly participants. Materials and Methods: Fifteen healthy elderly participants (ages, 55-84 years) were included in this study. High-resolution 3D T1-spoiled gradient recalled-echo (SPGR) images were obtained using 3T MRI. BSIM-based processing steps included an inhomogeneity correction, intensity normalization, skull stripping, atlas registration, extraction of intensity profiles, and calculation of cortical thickness. Processing steps were automatic, with the exception of semiautomatic skull stripping. Individual cortical thicknesses were compared to a database indicating mean cortical thickness of healthy adults, in order to produce Z-score thinning maps. Intra-class correlation coefficients (ICCs) were calculated in order to evaluate inter-rater and intra-rater reliabilities. Results: ICCs for intra-rater reliability were excellent, ranging from 0.751-0.940 in brain regions except the right occipital, left anterior cingulate, and left and right cerebellum (ICCs = 0.65-0.741). Although ICCs for inter-rater reliability were fair to excellent in most regions, poor inter-rater correlations were observed for the cingulate and occipital regions. Processing time, including manual skull stripping, was $17.07{\pm}3.43min$. Z-score maps for all participants indicated that cortical thicknesses were not significantly different from those in the comparison databases of healthy adults. Conclusion: BSIM-based cortical thickness measurements provide acceptable intra-rater and inter-rater reliability. We therefore suggest BSIM-based cortical thickness analysis as an adjunct clinical tool to detect cortical atrophy.

• 대한한의학회지
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• 제25권3호
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• pp.45-54
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• 2004

Objectives : This study was aimed to evaluate marker substances in Gami-Honghwa-Tang (KH-19) by high performance liquid chromatography-photodiode array detector (HPLC-DAD). Gami-Honghwa-Tang is composed of nine crude herbs, Rehmanniae Radix Preparata, Angelicae Gigantis Radix, Cnidii Rhizoma, Paeoniae Radix, Corni Fructus, Moutan Cortex Radicis, Lycii Fructus, Carthami Flos and Glycyrrhizae Radix. Methods : The separation was performed on an Aquasil C18 (4.6×250mm) column by gradient elution with 0.05% TFA in H2O - 0.05% TFA in acetonitrile (0 min 100:0, 20 min 90:10, 40 min 70:30, 60 min 50:50, 80 min 0:100, 90 min 100:0) as the mobile phase at a flow-rate of 1.0 ml/min with detection at 190-800nm. Also we examined the contents for bacteria, pesticide residue and harmful heavy metals. Results : HPLC-DAD was employed to determine the quantities and the qualities of several marker substances such as 5­hydroxymethyl-2-furaldehyde (5-HMF), paeonol, loganin, paeoniflorin, glycyrrhizin, and decursin in the KH-19. There were no bacterial contents, pesticide residues, or harmful heavy metals. Conclusions : We suggest these results could be a useful evidence for quality control of KH-19. This method permits fingerprints of selected individual marker substances from herbal prescriptions without derivatization, multiple purification steps, or lengthy separation times.

• 한국환경복원기술학회지
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• 제12권6호
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• pp.1-17
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• 2009

This study was carried out to classify vegetation structure of the Anseong district in the Deogyusan National Park from May 2007 to September 2008 using the gradient analyses and phytosociological method. The vegetation communities were classified into Quercus mongolica, Carpinus laxiflara, Q. serrata, Fraxinus mandshurica, Betula davurica, B. costata, Pinus densiflora and Abies koreana. Characteristics of the vegetation such as species composition, layer structure, vegetation ratio, and the distribution of individual trees by DBH were significantly different among communities. The order of important value of the forest community with DBH 2cm above plants was Q. mongolica (40.02), C. laxiflora (25.65), Q. serrata (24.68), F. mandshurica (21.66), P. densiflora (19.12), Acer pseudosieboldianum (16.96), C. cordata (11.87), B. davurica (11.66), B. costata (10.90), Styrax obassia (10.90) and A. koreana (10.41). Distribution of DBH P. densiflora had a formality distribution, suggesting a continuous domination of these species over the other species for the time being. In contrast, F. mandshurica appeared limited to the valley of the sheet and a higher frequency of young individuals, suggesting a continuous domination of these species the development of a climax forest terrain. This study showed the correlation between each community and the environment according to DCCA ordination. The A. koreana community predominated on the northern slope of the park in the high elevation habitats which had many O-M. F. mandshurica community predominated in the highest elevation habitats which had many Moisture, EX-Cation and T-N. The P. densiflora community mainly occurred on the southern slope of the park in the low elevation habitats which had few Moisture, O-M and T-N. The Q. serrata community and C. laxifiora community appeared on the park in the middle habitats.

• 한국지반공학회논문집
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• 제36권8호
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• pp.49-60
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• 2020

동상민감성 시료의 동상 거동을 평가하기 위해 완전히 결합된 열-수리-역학 연계해석을 수행하였다. 이를 위한 구성모델은 질량보존방정식, 에너지보존방정식, 힘평형방정식을 기반으로 유도되었다. 구성모델을 통해 간극수의 상변화, 간극수 유동 및 수반되는 기계적 변형 등 1차원 동결에 대한 다양한 물리적 현상을 정량적으로 고려할 수 있었다. 한편, 시료의 동상발생량 및 동상속도에 미치는 영향 인자들을 조사하기 위해 민감도 분석 연구가 수행되었다. 민감도 분석 결과에 따르면, 시료의 초기 간극비는 종속변수인 동상발생량과 동상속도에 독립적으로 큰 영향을 미치는 반면 흙 입자 열전도도 및 온도구배는 독립적으로 미치는 영향보다 두 변수 간 상호 작용을 통해 더 큰 영향을 미침을 확인하였다. 본 연구에서 고려된 인자들은 모두 동상발생량과 동상속도에 영향을 미치는 주요 인자이며, 표본시료의 동상민감성 여부를 결정하는 데에 활용될 수 있을 것이다.

• Investigative Magnetic Resonance Imaging
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• 제19권2호
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• pp.67-75
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• 2015

Purpose: To investigate the value of image post-processing software (FreeSurfer, IBASPM [individual brain atlases using statistical parametric mapping software]) and inversion time (TI) in volumetric analyses of the hippocampus and to identify differences in comparison with manual tracing. Materials and Methods: Brain images from 12 normal adults were acquired using magnetization prepared rapid acquisition gradient echo (MPRAGE) with a slice thickness of 1.3 mm and TI of 800, 900, 1000, and 1100 ms. Hippocampal volumes were measured using FreeSurfer, IBASPM and manual tracing. Statistical differences were examined using correlation analyses accounting for spatial interpretations percent volume overlap and percent volume difference. Results: FreeSurfer revealed a maximum percent volume overlap and maximum percent volume difference at TI = 800 ms ($77.1{\pm}2.9%$) and TI = 1100 ms ($13.1{\pm}2.1%$), respectively. The respective values for IBASPM were TI = 1100 ms ($55.3{\pm}9.1%$) and TI = 800 ms ($43.1{\pm}10.7%$). FreeSurfer presented a higher correlation than IBASPM but it was not statistically significant. Conclusion: FreeSurfer performed better in volumetric determination than IBASPM. Given the subjective nature of manual tracing, automated image acquisition and analysis image is accurate and preferable.

• Nuclear Engineering and Technology
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• 제42권6호
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• pp.670-679
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• 2010

In this paper, a new approach using a pixel-based correction method was developed to fix the non-uniform responses of flat-bed type scanners used for radiochromic film dosimetry. In order to validate the method's performance, two cases were tested: the first consisted of simple dose distributions delivered by a single port; the second was a complicated dose distribution composed of multiple beams. In the case of the simple individual dose condition, ten different doses, from 8.3 cGy to 307.1 cGy, were measured, horizontal profiles were analyzed using the pixel-based correcton method and compared with results measured by an ionization chamber and results corrected using the existing correction method. A complicated inverse pyramid dose distribution was made by piling up four different field shapes, which were measured with GAFCHROMIC$^{(R)}$EBT film and compared with the Monte Carlo calculation; as well as the dose distribution corrected using a conventional method. The results showed that a pixel-based correction method reduced dose difference from the reference measurement down to 1% in the flat dose distribution region or 2 mm in a steep dose gradient region compared to the reference data, which were ionization chamber measurement data for simple cases and the MC computed data for the complicated case, with an exception for very low doses of less than about 10 cGy in the simple case. Therefore, the pixel-based scanner correction method is expected to enhance the accuracy of GAFCHROMIC$^{(R)}$EBT film dosimetry, which is a widely used tool for two-dimensional dosimetry.