• Title/Summary/Keyword: Individual Identification

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Experimental Study for DNA Fingerprint from Teeth of Charred Body (소사체 치아에서의 유전자지문 분석을 위한 실험적 연구)

  • Jong-Hoon Choi
    • Journal of Oral Medicine and Pain
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    • v.21 no.2
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    • pp.351-367
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    • 1996
  • In the field Of individual identification in forensic Science, if the body is charred, it is sometimes impossible to identify the morphologic changes and charred tissue such as blood, muscle and bone can not be identified by forensic microbiologic method such as DNA typing. So the author used the characteristics of teeth which is relatively firm compare to other organs and stable to external environment such as heat and also possess cells needed for the DNA typing. The author conducted the experiment on teeth to detect DNA related to individual identification regarding to temperature in which other charredorgans can not be detected. The experiment was done on 64 extracted third molars consisted of unheated ones, and heated teeth to $100^{\circ}C$, $150^{\circ}C$, $200^{\circ}C$ for 45 min, 90 min, and 120 min respectively and to $250^{\circ}C$ for 45 min. DNA was extracted from each tooth and amplified fragment length polymorphism procedure(AMP-FLPs) using polymerase chain reaction(PCR) was applied and observed for the matching DNA in HumTH01 and HumCD4 locus and the followings Are the results : 1. It was able to detect matching DNA in HumTH01 and HumCD4 locus in every teeth which no heating has been done. 2. It was able to detect matching DNA in HumTH01 and HumCD4 locus in every teeth heated to $100^{\circ}C$ for 45, 90 and 120 min. 3. It was able to detect matching DNA in HumTH01 and HumCD4 locus in teeth heated to $l00^{\circ}C$, $200^{\circ}C$ for 45, 90, 120 min. 4. It was impossible to detect matching DNA in HumTH01 and HumCD4 locus in teeth heated to $250^{\circ}C$. So, it is possible to extract DNA from teeth that otherwise can not be extracted from other organs in the charred body and it can be concluded that teeth are highly reliable and applicatable as forensic odontology for individual identification.

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Acoustic Identification of Six Fish Species using an Artificial Neural Network (인공 신경망에 의한 6개 어종의 음향학적 식별)

  • Lee, Dae-Jae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.49 no.2
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    • pp.224-233
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    • 2016
  • The objective of this study was to develop an artificial neural network (ANN) model for the acoustic identification of commercially important fish species in Korea. A broadband echo acquisition and processing system operating over the frequency range of 85-225 kHz was used to collect and process species-specific, time-frequency feature images from six fish species: black rockfish Sebastes schlegeli, black scraper Thamnaconus modesutus [K], chub mackerel Scomber japonicus, goldeye rockfish Sebastes thompsoni, konoshiro gizzard shad Konosirus punctatus and large yellow croaker Larimichthys crocea. An ANN classifier was developed to identify fish species acoustically on the basis of only 100 dimension time-frequency features extracted by the principal components analysis (PCA). The overall mean identification rate for the six fish species was 88.5%, with individual identification rates of 76.6% for black rockfish, 82.8% for black scraper, 93.8% for chub mackerel, 90.6% for goldeye rockfish, 96.9% for konoshiro gizzard shad and 90.6% for large yellow croaker, respectively. These results demonstrate that individual live fish in well-controlled environments can be identified accurately by the proposed ANN model.

Biometric identification of Black Bengal goat: unique iris pattern matching system vs deep learning approach

  • Menalsh Laishram;Satyendra Nath Mandal;Avijit Haldar;Shubhajyoti Das;Santanu Bera;Rajarshi Samanta
    • Animal Bioscience
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    • v.36 no.6
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    • pp.980-989
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    • 2023
  • Objective: Iris pattern recognition system is well developed and practiced in human, however, there is a scarcity of information on application of iris recognition system in animals at the field conditions where the major challenge is to capture a high-quality iris image from a constantly moving non-cooperative animal even when restrained properly. The aim of the study was to validate and identify Black Bengal goat biometrically to improve animal management in its traceability system. Methods: Forty-nine healthy, disease free, 3 months±6 days old female Black Bengal goats were randomly selected at the farmer's field. Eye images were captured from the left eye of an individual goat at 3, 6, 9, and 12 months of age using a specialized camera made for human iris scanning. iGoat software was used for matching the same individual goats at 3, 6, 9, and 12 months of ages. Resnet152V2 deep learning algorithm was further applied on same image sets to predict matching percentages using only captured eye images without extracting their iris features. Results: The matching threshold computed within and between goats was 55%. The accuracies of template matching of goats at 3, 6, 9, and 12 months of ages were recorded as 81.63%, 90.24%, 44.44%, and 16.66%, respectively. As the accuracies of matching the goats at 9 and 12 months of ages were low and below the minimum threshold matching percentage, this process of iris pattern matching was not acceptable. The validation accuracies of resnet152V2 deep learning model were found 82.49%, 92.68%, 77.17%, and 87.76% for identification of goat at 3, 6, 9, and 12 months of ages, respectively after training the model. Conclusion: This study strongly supported that deep learning method using eye images could be used as a signature for biometric identification of an individual goat.

Application of DNA Test for Individual Traceability in the Brand Marketing of Korean Native Pig. (한국 재래돼지 브랜드 돈육 원산지 검증을 위한 유전자 원산지 감식 기법 활용 연구)

  • Choi, Bong-Am;Lee, Hak-Kyo;Jeon, Gwang-Joo;Oh, Jaen-Don;Choi, Il-Sin;Park, Mi-Hyun;Kong, Hong-Sik;Jung, Il-Jung;Kim, Tae-Hun;Yoon, Doo-Hak;Cho, Byung-Wook
    • Korean Journal of Organic Agriculture
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    • v.12 no.2
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    • pp.197-207
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    • 2004
  • Identification of animals has been used with an e ar tag with dummy code and blood typing has been used for paternity and individual identification in live animals. Various genetic markers are different for breeds of pig and hence, it is necessary to identity the discrete genetic marker in korean native pig. A total of 240 pigs were used to find korean native pig population specific markers that expressed in population of korean native pigs. To identify the individual traceability, 20 animals were randomly chosen and tested for a whole process from being live to slaughter stages. The candidate genetic marker used in the study were 18 DNA microsatellites which were identified in pig genome. The number of alleles of those DNA microsatellites ranged form a minimum of 3 to maximum of 6. The heterozygote frequency rang6d from 0.44 to 0.69. Effective number of alleles for each DNA microsatellotes were 2 to 4. By choosing 6 candidate genetic markers among all, the traceability of individual identification was estimated as accurate as 99.99%(p>0.0014), nearly.

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Factors related to Nurses' Patient Identification Behavior and the Moderating Effect of Person-organization Value Congruence Climate within Nursing Units (간호사의 환자확인 행동 관련 요인 및 개인-조직 가치일치 분위기의 상호작용 효과)

  • Kim, Young Mee;Kang, Seung-Wan;Kim, Se Young
    • Journal of Korean Academy of Nursing
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    • v.44 no.2
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    • pp.198-208
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    • 2014
  • Purpose: This research was an empirical study designed to identify precursors and interaction effects related to nurses' patient identification behavior. A multilevel analysis methodology was used. Methods: A self-report survey was administered to registered nurses (RNs) of a university hospital in South Korea. Of the questionnaires, 1114 were analyzed. Results: The individual-level factors that had a significantly positive association with patient identification behavior were person-organization value congruence, organizational commitment, occupational commitment, tenure at the hospital, and tenure at the unit. Significantly negative group-level precursors of patient identification behavior were burnout climate and the number of RNs. Two interaction effects of the person-organization value congruence climate were identified. The first was a group-level moderating effect in which the negative relationship between the number of RNs and patient identification behavior was weaker when the nursing unit's value congruence climate was high. The second was a cross-level moderating effect in which the positive relationship between tenure at the unit and patient identification behavior was weaker when value congruence climate was high. Conclusion: This study simultaneously tested both individual-level and group-level factors that potentially influence patient identification behavior and identified the moderating role of person-organization value congruence climate. Implications of these results are discussed.

Establishment of Genetic Characteristics and Individual Identification System Using Microsatellite Loci in Jeju Native Horse (초위성체 DNA를 이용한 제주마 집단의 품종특성 및 개체식별 체계설정)

  • Cho, Byung-Wook;Jung, Ji-Hye;Kim, Sang-Wook;Kim, Heui-Soo;Lee, Hak-Kyo;Cho, Gil-Jae;Song, Ki-Duk
    • Journal of Life Science
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    • v.17 no.10
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    • pp.1441-1446
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    • 2007
  • This study was conducted to establish the individual identification system and to estimate the genetic characteristic of Jeju native horse (JNH) using 13 microsatellite markers located on different horse autosomes. The markers were genotyped on 191 animals from five horse breeds including Jeju native horse (JNH). In total, 138 alleles were detected from the genotypes of 13 microsatellite markers. The average heterozygosities ranged from 0.317 to 0.902 and the polymorphic information content (PIC) ranged from 0.498 to 0.799 in JNH. We found that there were significant differences in allele frequencies in JNH when compared with other horse breeds. In ATH4 marker, there were specific allele frequence pattern that some of allele only found in JNH, Mongolian horse (MONG) and Jeju racing horse (JRH). The calculated cumulative power of discrimination (CPD) was 99.9% when nine microsatellite loci were used for analysis in the individual identification system. Also, the matching probability that two unrelated animals would show the same genotypes, was estimated to be $0.60{\times}10^{-10}$. Therefore, in the nine markers used in this study can be used for individual identification in the Jeju native horse population.

A Study on Speaker Identification by Difference Sum and Correlation Coefficients of Narrow-band Spectrum (좁은대역 스펙트럼의 차이값과 상관계수에 의한 화자확인 연구)

  • Yang, Byung-Gon;Kang, Sun-Mee
    • Speech Sciences
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    • v.9 no.3
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    • pp.3-16
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    • 2002
  • We examined some problems in speaker identification procedures: transformation of acoustic parameters into auditory scales, invalid measurement values, and comparability of spectral energy values across the frequency range. To resolve those problems, we analyzed the acoustic spectral energy of three Korean numbers produced by ten female students from narrow-band spectrograms at 19 proportional time points of each voiced segment. Then, cells of the first five spectral matrices were averaged to form a matrix model for each speaker. The correlation coefficients and sum of the absolute amplitude difference in each pair of the spectral models of the ten subjects were obtained. Also, some individual matrix models were compared to those of the same subject or the other subject with a similar spectral model. Results showed that in numbers '2' and '9' subjects could not be clearly distinguished from the others but in number '4' it shed some possibility of setting threshold values for speaker identification if we employed the coefficients and the sum of absolute difference. Further studies would be desirable on various combinations of the range of long-term average spectra and the degree of signal pre-emphasis.

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The Identification of Japanese Black Cattle by Their Faces

  • Kim, Hyeon T.;Ikeda, Y.;Choi, Hong L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.6
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    • pp.868-872
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    • 2005
  • Individual management of the animal is the first step towards reaching the goal of precision livestock farming that aids animal welfare. Accurate recognition of each individual animal is important for precise management. Electronic identification of cattle, usually referred to as RFID (Radio Frequency Identification), has many advantages for farm management. In practice, however, RFID implementations can cause several problems. Reading speed and distance must be optimized for specific applications. Image processing is more effective than RFID for the development of precision farming system in livestock. Therefore, the aim of this paper is to attempt the identification of cattle by using image processing. The majority of the research on the identification of cattle by using image processing has been for the black-and-white patterns of the Holstein. But, native Japanese and Korean cattle do not have a consistent pattern on the body, so that identification by pattern is impossible. This research aims to identify to Japanese black cattle, which does not have a black-white pattern on the body, by using image processing and a neural network algorithm. 12 Japanese black cattle were tested. Values of input parameter were calculated by using the face image values of 12 cows. The face was identified by the associate neural memory algorithm, and the algorithm was verified by the transformed face image, for example, of brightness, distortion, noise and angle. As a result, there was difference due to a transformation ratio of the brightness, distortion, noise, and angle. The algorithm could identify 100% in the range from -30 to +30 degrees of brightness, -20 to +40 degrees of distortion, 0 to 60% of noise and -20 to +30 degree of angle transformed images.

Correlations between Sperm Motility, SCSA (Sperm Chromatin Structure Assay), Reproductive Performance and Heterospermic Fertility in Boars

  • Kim, In-Cheul;Ryu, Jae-Weon;Cho, Kyu-Ho;Hong, Joon-Ki;Choi, Eun-Ji;Choi, Bong-Hwan;Park, Jun-Cheol;Moon, Hong-Kil;Son, Jung-Ho
    • Reproductive and Developmental Biology
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    • v.32 no.2
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    • pp.127-133
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    • 2008
  • The objective of this study was two folds: to investigate the relationship between paternal identification rate and sperm quality parameters such as motility and sperm chromatin structure assay after heterospermic insemination; to see if mutual complement between tests and development of useful technique to enhance the fertility in artificial insemination. In individual boar's fertilizing ability, 3 high fertility boars showed significantly high fertility (p<0.05) compared to 3 low fertility boars, but there was no difference in litter size between two groups. Sperm motility test in pooled and individual semen using computer assisted sperm analysis (CASA) revealed that no significant difference among boars. The high fertile boar showed tendency of low %Red (High red fluorescence/green+red fluorescence) in sperm chromatin structure assay (SCSA) but paternal identification rate from piglets did not differ after heterospermic insemination. The correlation coefficient between individual or pooled semen function test and farrowing rates were well correlated as follows: %Red with litter size (r= - 0.53, p=0.03); %Red with paternal identification rates (r=-0.51, p=0.03); paternal identification rates with litter size (r=0.57, p=0.02). These results indicate that sperm chromatin structure assay and sperm quality parameter test in pooled semen are useful method to predict and evaluate the fertilizing capacity after heterospermic insemination in boars.

Consideration to The Role of Forensic Odontology in Determination of Unidentified Human Body (신원불상자의 개인식별에서의 치아의 역할에 대한 고찰)

  • Ahn, Yong-Woo;Ko, Chul-Hee;Huh, Ki-Yeong;Lee, Sang-Seob;Ok, Soo-Min;Huh, Joon-Young;Ko, Myung-Yun;Jeong, Sung-Hee
    • Journal of Oral Medicine and Pain
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    • v.35 no.2
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    • pp.111-117
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    • 2010
  • Forensic odontology in individual identification has been progressing rapidly and its significance has been increasing. But not all individual identification can be done single method solely. Therefore, understanding the advantage and disadvantage of each method is necessary to achieve forensic individual identification properly in each case for it to be applied promptly, economically and precisely. Now We have identified almost 20 case in Busan and Kyeong Nam area in 2008, 2009 and we would like to report some of cases. Finally We will consider role of forensic odontology.