• Title/Summary/Keyword: Indirect Method

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Near Infrared Spectroscopy for Measuring Purine Derivatives in Urine and Estimation of Microbial Protein Synthesis in the Rumen for Sheep

  • Atanassova, Stefka;Iancheva, Nana;Tsenkova, Roumiana
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1273-1273
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    • 2001
  • The efficiency of the luminal fermentation process influences overall efficiency of luminal production, animal health and reproduction. Ruminant production systems have a significant impact on the global environment, as well. Animal wastes contribute to pollution of the environment as ammonia volatilized to the air and nitrate leached to ground water. Microbial protein synthesis in the rumen satisfies a large proportion of the protein requirements of animals. Quantifying the microbial synthesis is possible by using markers for lumen bacteria and protozoa such as nucleic acids, purine bases, some specific amino acids, or by isotopic $^{15}N,^{32}P,\;and\;^{35}S$ labelled feeds. All those methods require cannulated animals, they are time-consuming and some methods are very expensive as well. Many attempts have been made to find an alternative method for indirect measurement of microbial synthesis in intact animals. The present investigations aimed to assess possibilities of NIRS for prediction of purine nitrogen excretion and ruminal microbial nitrogen synthesis by NIR spectra of urine. Urine samples were collected from 12 growing sheep,6 of them male, and 6- female. The sheep were included in feeding experiment. The ration consisted of sorghum silage and protein supplements -70:30 on dry matter basis. The protein supplements were chosen to differ in protein degradability. The urine samples were collected daily in a vessel containing $60m{\ell}$ 10% sulphuric acid to reduce pH below 3 and diluted with tap water to 4 liters. Samples were stored in plastic bottles and frozen at $-20^{\circ}C$ until chemical and NIRS analysis. The urine samples were analyzed for purine derivates - allantoin, uric acid, xantine and hypoxantine content. Microbial nitrogen synthesis in the lumen was calculated according to Chen and Gomes, 1995. Transmittance urine spectra with sample thickness 1mm were obtained by NIR System 6500 spectrophotometer in the spectral range 1100-2500nm. The calibration was performed using ISI software and PLS regression, respectively. The following statistical results of NIRS calibration for prediction of purine derivatives and microbial protein synthesis were obtained.(Table Omitted). The result of estimation of purine nitrogen excretion and microbial protein synthesis by NIR spectra of urine showed accuracy, adequate for rapid evaluation of microbial protein synthesis for a large number of animals and different diets. The results indicate that the advantages of the NIRS technology can be extended into animal physiological studies. The fast and low cost NIRS analyses could be used with no significant loss of accuracy when microbial protein synthesis in the lumen and the microbial protein flow in the duodenum are to be assessed by NIRS.

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Transforming Growth Factor-Beta Stimulates Osteoclastic Bone Resorption in vitro (파골세포에 대한 Transforming Growth Factor-$\beta$의 활성화 작용)

  • 양대석;김일찬;고성희;유병제;남궁용;강신성;이창호
    • The Korean Journal of Zoology
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    • v.39 no.3
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    • pp.317-324
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    • 1996
  • Osteoclast has been known as a primary responsible cell for the bone resorption. The activation of osteoclast, therefore, may be the key event in the regulation of bone growth and remodeling. Various factors were reported to have influence on the resorbing activity of osteoclast in organ culture. Among those factors, transforming growth factor-$\beta$ (TGF-$\beta$) has been known to have a profound effect on bone metabolism. Since a large amount of TGF-$\beta$ presents in bone tissue, it may be important for the understanding the regulatory mechanism of bone resorption to elucidate the effect of TGF-$\beta$ on the osteoclast. We have reported the dlsaggregated chick embryonic osteoclast culture as an useful assay method for determining the resorption activity of osteoclast. In this culture, we found that TGF-$\beta$ significantly enhaced the osteoclastic bone resorption activity. We also found that the timulatory effect seemed to be an indirect one that is mediated by other cells. As nordihydroguaiaretic acid significantly inhibited the TGF-$\beta$1-induced osteoclastic bone resorption, we suggest that the lipoxygenase derivative of arachidonic acid may participate in the action of TGF-$\beta$ as a paracrine or an autocrine mediator.

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Prediction of Ground Condition and Evaluation of its Uncertainty by Simulated Annealing (모의 담금질 기법을 이용한 지반 조건 추정 및 불확실성 평가에 관한 연구)

  • Ryu Dong-Woo
    • Tunnel and Underground Space
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    • v.15 no.4 s.57
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    • pp.275-287
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    • 2005
  • At the planning and design stages of a development of underground space or tunneling project, the information regarding ground conditions is very important to enhance economical efficiency and overall safety In general, the information can be expressed using RMR or Q-system and with the geophysical exploration image. RMR or Q-system can provide direct information of rock mass in a local scale for the design scheme. Oppositely, the image of geophysical exploration can provide an exthaustive but indirect information. These two types of the information have inherent uncertainties from various sources and are given in different scales and with their own physical meanings. Recently, RMR has been estimated in unsampled areas based on given data using geostatistical methods like Kriging and conditional simulation. In this study, simulated annealing(SA) is applied to overcome the shortcomings of Kriging methods or conditional simulations just using a primary variable. Using this technique, RMR and the image of geophysical exploration can be integrated to construct the spatial distribution of RM and to evaluate its uncertainty. The SA method was applied to solve an optimization problem with constraints. We have suggested the practical procedure of the SA technique for the uncertainty evaluation of RMR and also demonstrated this technique through an application, where it was used to identify the spatial distribution of RMR and quantify the uncertainty. For a geotechnical application, the objective functions of SA are defined using statistical models of RMR and the correlations between RMR and the reference image. The applicability and validity of this application are examined and then the result of uncertainty evaluation can be used to optimize the tunnel layout.

Performance of Occupational Health Services by Type of Service (사업장 보건관리사업의 형태별 수행성과 분석 -실적, 수혜도, 영향요인을 중심으로-)

  • Cho, Tong-Ran
    • Research in Community and Public Health Nursing
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    • v.6 no.1
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    • pp.34-54
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    • 1995
  • Occupational health services in Korea have been operated as dual types: one is operated by occupational health care manager and the other is health care agency without their own personnel. The performance of occupational health service should be different due to the variety of characteristics of health care manager and workplace, qualification of health care manager. This study is to analyze performance of occupational health care services with a particular consideration of job, based on comparing those two types of health care management to show on the basic data for the settlement of more qualitative. health care management system at workplace. For this study, total 391 places in Seoul and Inchon city area: 154 places (39.4%) managed by designated. health care manager and 237 places (60.6%) by the agency with their commission are selected as research samples. Tools for data collection are questionnares have been investigated during the period of 20 September 1993-20 December 1993. Those data are compared with percentiles, mean, standard deviation due to the characterstics of each variable and analyzed for impacting factors with relation to the using multiple regression analysis using SPSS PC program, especially using t -test method in order to compare each type of health care management. Conclusions observed from the tests and each comparison could be summerized as follows : 1. Occupational health care have been accomplished at workplaces with designated people than with agencies people, and coverage rate of the occupational health care services has differences, due to management types. The reason of these results is due to visit only one or two times monthly by the agencies, while their own health care manager obsess, at the workplaces all the times. 2. The common sickness management is the most accomplished item in health care area of occupational health care services, while the preventive care and control for the workers who have serious health problems are insufficient in workers health care area. 3. An insufficient accomplishment of overall health education has been shown because it is difficult to perform health education due to almost no chance of the direct introduction at workplaces. Therefore a strong support system for making and supplying the media is necessary in order to activate indirect health education by means of media. 4. Because health care managers and the agencies managers where take the workplaces for this study are almost nurses who have been comparatively high work site rounding rate about an environmental management at the workplaces, that non-profession can also do it, the activities about the professional area not enough. Therefore, an appropriate referral system should be established in order to complement professional area. 5. Two factors which have an effect on the coverage rate of occupational health care services are : one is those from the workplaces such as type of services, the number of workers, the number of harzadous factors and safety & health committee, the other from health care organization about whether there is its own manager or not.

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Effects of Mentoring Function on Career Development and Organizational Effectiveness in Private Security Service Organization (경호경비조직의 멘토링기능이 경력개발 및 조직유효성에 미치는 영향)

  • Yoo, Young-Chang;Kim, Hyo-Joon;Kim, Chan-Sun
    • The Journal of the Korea Contents Association
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    • v.11 no.6
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    • pp.326-336
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    • 2011
  • The Purpose of this study was to examine effects of mentoring function on career development and organizational effectiveness in private security service organization. Example number used on interpretation finally using purposive sampling method after this study establishes 5 places private security service company's security guard by population in Seoul on March, 2010 is total 227 people. Reliability of questionnaire appeared Cronbach's ${\alpha}$ value more than .667. Conclusions that appears in this study is as following. First, private security service organization's Mentoring function affects in career development. That is, if friendship, patronage, career management, society mind, and role model are mobilized, innovative own development, special capacity development, and information competitive power development are helped. Second, private security service organization's Mentoring function affects in organization effectiveness. If friendship, patronage, career management, society mind, and role model are mobilized, career satisfaction, organization immersion, and job satisfaction increase. On the other hand, change of jobs intention decreases if function of society mind is mobilized. Third, private security service organization's career development affects in organization effectiveness. If reform and special capacity development are helped, career satisfaction, organization immersion, and job satisfaction increase. While change of jobs intention decreases as development of information competition is helped. Fourth, Mentoring function exerts influence of causality on career development and organization effectiveness. That is, Mentoring exerts direct influence on organization effectiveness, but it exerts indirect effect through career development.

An Experimental Research on the Usability of Indirect Control using Finger Gesture Interaction in Three Dimensional Space (3차원 공간에서 손가락 제스쳐 인터랙션을 이용한 간접제어의 사용성에 관한 실험연구)

  • Ham, Kyung Sun;Lee, Dahye;Hong, Hee Jung;Park, Sungjae;Kim, Jinwoo
    • The Journal of the Korea Contents Association
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    • v.14 no.11
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    • pp.519-532
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    • 2014
  • The emerging technologies for the natural computer interaction can give manufacturers new opportunities of product innovation. This paper is the study on a method of human communication about a finger gestures interaction. As technological advance has been so rapid over the last few decades, the utilizing products or services will be soon popular. The purpose of this experiment are as follows; What is the usefulness of gesture interaction? What is the cognitive impact on gesture interaction users. The finger gestures interaction consist of poking, picking and grasping. By measuring each usability in 2D and 3D space, this study shows the effect of finger gestures interaction. The 2D and 3D experimental tool is developed by using LeapMotion technology. As a results, the experiments involved 48 subjects shows that there is no difference in usability between the gestures in 2D space but in 3D space, the meaningful difference has been found. In addition, all gestures express good usability in 2D space rather than 3D space. Especially, there are the attractive interest that using uni-finger is better than multi-fingers.

Relationship between Internal Service Quality and Internal Customer Satisfaction, Management Outcome in Airport Private Security Agents (공항 특수경비조직의 내부서비스품질과 내부고객만족 및 경영성과의 관계)

  • Kim, Chan-Sun;Lee, Ji-Eun;Jo, Byung-Hae
    • The Journal of the Korea Contents Association
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    • v.12 no.4
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    • pp.426-437
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    • 2012
  • The purpose of this study is to investigate the relationship between internal service quality, internal customer satisfaction and management outcome of airport security agents. This study had selected special securities from Incheon airport of October, 2011 for population. Using judgment sampling method, 282 samples were drawn and were used for the final analysis. Questionnaire used in study is composed of total 43 questions and using SPSSWIN 18.0, frequency analysis, factor analysis, reliability analysis, multiple regression analysis and path analysis were performed. Cronbach's ${\alpha}$ value which shows the reliability of the survey came out to be over .686. The conclusion is following: First, internal service quality of airport security agents affects internal customer satisfaction. That is, as corporality, responsiveness and empathy increase, internal supply satisfaction and job satisfaction also increase. Second, internal service quality of airport security agent affects job performance. That is, as corporality, reliability and responsiveness increase, non-financial performance and financial performance also increase. However, when empathy is not activated well, financial performance is decreased. Third, airport security agent…s internal customer satisfaction affects job performance. That is, as activation of internal supply satisfaction increases, nonfinancial performance and financial performance are increased. Fourth, airport special security agent…s inside quality of service exerts direction indirect effect in inside customers satisfaction and management result. That is, inside customers satisfaction is an important variable that mediate inside quality of service and management result.

A Study on the substance of Eum-Fire(陰火) in Li Dong-yuan(李東垣)'s Eum-Fire theory (이동원(李東垣)이 논한 음화(陰火)의 실질(實質)에 대한 연구)

  • Eun, Seok-Min
    • Journal of Korean Medical classics
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    • v.25 no.4
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    • pp.5-22
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    • 2012
  • Objective : The concept of Eum-Fire(陰火), which was brought up by Li Dong-yuan(李東垣), is such a very ambiguous thing that many medical scholars had tried to understand its real meaning. Trying to understand the real concept of Eum-Fire, this study goes on based on the view that the concept of Eum-Fire in the medical books of Li Dong-yuan was used as being the same thing with the concept of Xim-Fire(心火) and Premier-Fire (相火), and the substance of this kind of fire is the fire in Ximpo(心包) and Myungmun (命門) which works in the system of Ximgye(心系). To verify this view is the main objective of this study. Method : Inquiring into Li Dong-yuan's medical books, this study investigated examples of the uses of the terms of Eum-Fire, Xim-Fire and Premier-Fire, and analyzed the relation between the meanings of these terms. And as a collateral evidence to the main view of this study, this study notes Wang An-dao(王安道)'s reference about Eum-Fire and inquires into the concept of Premier-Fire that was formed by Liu He-jian(劉河間), who had been referred to in Wang An-dao's argument about Eum-Fire. And this study also presents the Chen Shi-duo(陳士鐸)'s view about Eum-Fire that is expected to show a more concrete concept of Eum-Fire than now and seems to deserve to be compared with that of Li Dong-yuan. Result and Conclusion : It could be understood that the concept of Eum-Fire in Li Dong-yuan's books was used as a thing that owns the same substance as a kind of fire with Xim-Fire and Premier-Fire. In this viewpoint it could also be understood that Li Dong-yuan had attached the concepts like Xim-Fire and Eum-Fire to the concept of Premier-Fire, so terms like these could be understood as such concepts that represented the diverse figures of Premier-Fire. And this study also found that the substance of Xim-Fire as a kind of Premier-Fire means the fire of Ximpo and Myungmun that works in the system of Ximgye, which seems to be formerly introduced as a frame of Premier-Fire by Liu He-jian. This study also found the indirect basis of this viewpoint in the books of Chen Shi-duo, and could get a conclusion that Chen Shi-duo's view about Eum-Fire can be regarded as a thing that could show us more concrete matter of Eum-Fire than now.

Breast Cancer Clustering in Kanagawa, Japan: A Geographic Analysis

  • Katayama, Kayoko;Yokoyama, Kazuhito;Yako-Suketomo, Hiroko;Okamoto, Naoyuki;Tango, Toshiro;Inaba, Yutaka
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.1
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    • pp.455-460
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    • 2014
  • Background: The purpose of the present study was to determine geographic clustering of breast cancer incidence in Kanagawa Prefecture, using cancer registry data. The study also aimed at examining the association between socio-economic factors and any identified cluster. Materials and Methods: Incidence data were collected for women who were first diagnosed with breast cancer during the period from January to December 2006 in Kanagawa. The data consisted of 2,326 incidence cases extracted from the total of 34,323 Kanagawa Cancer Registration data issued in 2011. To adjust for differences in age distribution, the standardized mortality ratio (SMR) and the standardized incidence ratio (SIR) of breast cancer were calculated for each of 56 municipalities (e.g., city, special ward, town, and village) in Kanagawa by an indirect method using Kanagawa female population data. Spatial scan statistics were used to detect any area of elevated risk as a cluster for breast cancer deaths and/or incidences. The Student t-test was performed to examine differences in socio-economic variables, viz, persons per household, total fertility rate, age at first marriage for women, and marriage rate, between cluster and other regions. Results: There was a statistically significant cluster of breast cancer incidence (p=0.001) composed of 11 municipalities in southeastern area of Kanagawa Prefecture, whose SIR was 35 percent higher than that of the remainder of Kanagawa Prefecture. In this cluster, average value of age at first-marriage for women was significantly higher than in the rest of Kanagawa (p=0.017). No statistically significant clusters of breast cancer deaths were detected (p=0.53). Conclusions: There was a statistically significant cluster of high breast cancer incidence in southeastern area of Kanagawa Prefecture. It was suggested that the cluster region was related to the tendency to marry later. This study methodology will be helpful in the analysis of geographical disparities in cancer deaths and incidence.

Fabrication and estimation of the plastic detector for measuring the contamination for beta-ray level of the kind of duct waste (배관류 폐기물의 베타선 오염도 측정용 플라스틱 검출기 제작 및 특성평가)

  • Kim Gye-Hong;Oh Won-Zin;Lee Kune-Woo;Seo Bum-Kyoung
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.3 no.3
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    • pp.159-165
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    • 2005
  • The characterization of radiological contamination inside pipes generated during the decommission of a nuclear facility is necessary before pipes can be recycled or disposed. But, existing direct measurements of radioactive contamination level using the survey-meter can not estimate the characteristic of contamination on a local area such as the pipe inside. Moreover, the measurement of surface contamination level using the indirect methods has many problems of an application because of the difficulty of collecting sample and contamination possibility of a worker when collecting sample. In this work, plastic scintillator was simulated by using Monte Carlo simulation method for detection of beta radiation emitted from internal surfaces of small diameter pipe. Simulation results predicted the optimum thickness and geometry of plastic scintillator at which energy absorption for beta radiation was maximized. In addition, the problem of scintillator processing and transferring the detector into the pipe inside was considered when fabricating the plastic detector on the basis of simulation results. The characteristic of detector fabricated was also estimated. As a result, it was confirmed that detector capability was suitable for the measurement of contamination level. Also, the development of a detector for estimating the radiological characteristic of contamination on a local area such as the pipe inside was proven to be feasible.

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