• 한국지하수토양환경학회지:지하수토양환경
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• 제17권3호
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• pp.49-58
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• 2012

Batch experiments using indigenous and commercialized adventive microorganisms were performed to investigate the feasibility of the biodegradation process for the diesel contaminated soil, which was taken in US Military Camp 'Hialeah', Korea. TPH concentration of the soil was determined as 3,819 mg/kg. Four indigenous microorganisms having high TPH degradation activity were isolated from the soil and by 16S rRNA gene sequence analysis, they were identified as Arthrobacter sp., Burkholderia sp., Cupriavidus sp. and Bacillus sp.. Two kinds of commercialized solutions cultured with adventive microorganisms were also used for the experiments. Various biodegradation conditions such as the amount of microorganism, water content and the temperature were applied to decide the optimal bioavailability condition in the experiments. In the case of soils without additional microorganisms (on the natural attenuation condition), 35% of initial TPH was removed from the soil by inhabitant microorganisms in soil for 30 days. When the commercialized microorganism cultured solutions were added into the soil, their average TPH removal efficiencies were 64%, and 54%, respectively, which were higher than that without additional microorganisms. When indigenous microorganisms isolated from the contaminated soil were added into the soil, TPH removal efficiency increased up to 95% (for Bacillus sp.). According to the calculation of the average biodegradation rates for Bacillus sp., the remediation goal (87% of the removal efficiency: 500 mg/kg) for the soil would reach within 24 days. Results suggested that TPH removal efficiency of biodegradation by injecting indigenous microorganisms is better than those by injecting commercialized adventive microorganisms and only by using the natural attenuation.

• Mycobiology
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• 제48권5호
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• pp.392-398
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• 2020

This study was conducted to understand the dynamics of microbial communities of soil microorganisms, and their distribution and abundance in the indigenous microorganisms (IMOs) manipulated from humus collected from the forest near the crop field. The soil microorganisms originated from humus and artificially cultured microbial-based soil amendments were characterized by molecular and biochemical analyses. The bacterial population (2 × 10⁶~13 × 10⁶ CFU/g sample) was approximately 100-fold abundant than the fungal population (2 × 10⁴~8 × 10⁴ CFU/g sample). The 16S rDNA and ITS sequence analyses showed that the bacterial and fungal communities in humus and IMOs were mainly composed of Bacillus and Pseudomonas, and Trichoderma and Aspergillus species, respectively. Some of the bacterial isolates from the humus and IMOs showed strong inhibitory activity against soil-borne pathogenic fungi Fusarium oxysporum and Sclerotinia sclerotiorum. These bacteria also showed the siderophore production activity as well as phosphate solubilizing activity, which are requisite traits for biological control of plant pathogenic fungi. These results suggest that humus and IMOs could be a useful resource for sustainable agriculture.

• 한국균학회지
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• 제49권4호
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• pp.433-440
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• 2021

The fungal genus Trametes is globally distributed and comprises various wood-decay species, including the well-known medicinal mushroom Trametes versicolor, a popular remedy in traditional Asian medicine. Trametes species produce antioxidants, which have a wide range of health benefits. The pressent study evaluated seven indigenous Trametes species from Korea, which were cultivated in three different media (dextrose-yeast extract, DY; malt extract-yeast extract, MY; malt extract broth, MEB) and tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. We found that the medium consumption rate did not significantly differ between the media and among the strains (72-76%). However, the T. versicolor strains had a relatively low consumption rate (14-65%). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) tests demonstrated that culture filtrates of T. cf. junipericola, T. orientalis, T. suaveolens, and T. versicolor possessed antioxidant activity against damage from free radicals. In particular, T. cf. junipericola (DY) and T. versicolor (MY) had >80% activity in the DPPH and ABTS assays, compared with that of the positive control (ascorbic acid). Thus, our study identified promising candidates with substantial antioxidant activity among the indigenous strains of Trametes spp. from Korea.

• 한국균학회지
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• 제49권2호
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• pp.211-242
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• 2021

국내 효모연구는 1910년 이래로 현재까지 주류, 식품, 환경 등의 분야에서 활발히 진행되고 있다. 그러나 국내에 서식하는 효모의 종 수와 목록에 대해서는 연구된 바가 없다. 한국에서 보고된 문헌에 기재된 효모 종과 균주센터가 보유하고 있는 효모를 조사한 결과 128속 681종(26변종 포함)이 국내에서 보고된 효모 종 수로 나타났다. 발표종 681종에 대한 분류학적 재검토 결과 142속 500종(9변종 포함)으로 확인되었다. 이 중 국가생물종목록에 기재되지 않아 새롭게 등록가능성이 있는 종은 334종이다. 국가생물종목록은 국내 서식 생물의 다양성 보전 및 관리에 활용되는 자료로, 국가 생물자원의 주권확보라는 측면에서 매우 중요하다. 따라서 국내에 서식하는 것으로 보고된 효모 종의 문헌과 균주에 대한 추가 검증 연구를 통해 국가생물종목록에 등록하는 후속 연구가 필요하다.

• Journal of Applied Biological Chemistry
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• 제42권1호
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• pp.7-11
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• 1999

An extracellular chitinase of the selected strong antifungal microorganism, Serratia sp. 3095, was purified by salting out, affinity adsorption, Sepadex G-100 gel fitration, Sepadex G-75 gel fitration and DEAE Sepadex A-50 chromatography. The molecular weight of the purified chitinase was estimated to be 62,000 dalton by SDS-PAGE. Optimal pH and temperature of the chitinase were pH 7.5 and 45, respectively. The enzyme retained more than 80% of the activity between pH 5.5 and pH 10.5, and below $50^{\circ}C$ but was unstable above $60^{\circ}C$, below pH 5.0. The activity of the chitinase was inhibited about 60% by $Sn^{2+}$, 40% by $Hg^{2+}$ and $Ag^+$, 70% by AHA, 40% by iodoacetate, 35% by thiourea and p-CMB, but stabilized by SDS. $K_m$ value of the purified chitinase was 3.68 mg/ml for colloidal chitin. The chitinase from Serratia sp. 3095 showed antifungal activity to Fusariurm solani.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 총회 및 춘계학술발표회
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• pp.162-166
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• 2004

This study investigated tile effect of ozonation on indigenous microorganisms distributed in different size fractions of soil aggregates. Soil was ozonated from 0 to 300 minutes. The treated soils were fractionated into 3 groups (small, <53 $\mu$m; medium, 53-500 $\mu$m; and large, 2000-500 $\mu$m) and total heterotrophic bacteria in the soils were enumerated. Cell number decreased rapidly within 120 minute ozonation and showed slow decrease upon longer ozonation. Abundance of total heterotrophic bacteria in each fraction was in the following order regardless of ozonation time: small>medium>large fractions. Difference in microbial abundance among the fractions was smaller as ozonation time increased.

• Mycobiology
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• 제49권5호
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• pp.454-460
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• 2021

Daecheongdo and Socheongdo Islands are located in the West Sea of Korea, 210 km away from land, and are military border areas very close to North Korea, making them difficult to access. Although the ecosystem of the islands is relatively well preserved due to the lack of accessibility, research on fungi of the regions is insufficient. Therefore, we aimed to investigate indigenous fungi in these geographically and geopolitically constrained regions. A survey of the indigenous fungal diversity of the islands was conducted in 2018. All specimens were identified at the species level based on morphological and molecular analyses. Among them, six macrofungi-namely, Agaricus menieri, Crepidotus praecipuus, Dichomitus squalens, Hortiboletus amygdalinus, Melanoleuca friesii, and Trametes lactinea-were not previously reported in Korea. Considering that the proportion of unrecorded species is high in the survey area and period as well as the number of samples collected, similar research on adjacent islands may be necessary.

• Journal of Species Research
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• 제11권4호
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• pp.310-320
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• 2022

As a subset study to discover indigenous prokaryotic species in Korea, we isolated 20 bacterial strains and assigned them to the phyla Actinobacteria, Bacteroidota, Firmicutes, and Proteobacteria. From the high 16S rRNA gene sequence similarity (≥98.7%) and formation of a robust phylogenetic clades, we determined that each strain belonged to independent, predefined bacterial species. There are no official reports of these 20 species in Korea; therefore, 7 strains of the Actinobacteria, 2 strain of the Bacteroidota, 3 strains of the Firmicutes, and 8 strains of the Firmicutes are described in Korea for the first time. Gram reaction, colony and cell morphology, basic biochemical characteristics, and isolation sources are also described in the species description section.

• 대한환경공학회지
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• 제31권1호
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• pp.35-41
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• 2009

본 연구는 토착미생물에 의한 발효기작을 이용하여 음식물쓰레기를 동물 사료로 이용하기 위한 가능성을 평가하기 위하여 수행되었다. 이를 위하여 효소활성 실험을 통하여 anylase, protease, lipase에 대한 우수활성을 나타내는 토착유효미생물을 토양으로부터 분리하여 음식물쓰레기의 사료화를 위한 발효제로 사용하였다. 16s rDNA sequencing의 분석을 통해 우수활성을 나타내는 미생물(H1, D1)은 Bacillus subtilis와 Paenibacillus polymyxa인 것으로 확인되었다. 사료화 실험에서는 수분과 영양소 함량의 조절을 위하여 쌀겨 및 팽화미를 음식물쓰레기에 혼합하여 사료화를 위한 시료로 사용하였으며 효소활성 실험을 통해 분리된 2종의 미생물(Bacillus subtilis, Paenibacillus polymyxa)을 발효제로 사용하여 사료화 실험을 진행하였다. 발효실험 동안의 온도, pH 및 ORP 측정을 통해 토착유효미생물의 접종에 의한 음식물쓰레기의 발효가 효과적으로 진행됨을 확인할 수 있었다. 반응종료후 제조된 사료의 영양분 분석 결과에서 조단백질, 조지방, 조섬유은 동물 급여에 충분한 함량을 나타내었다. 또한 Pb, Hg, Cd, aflatoxin 및 salmonella 등의 유해물질의 분석결과 모든 항목이 허용기준치 이하의 값을 나타내었다. 따라서 토착유효미생물을 이용한 음식물쓰레기의 사료화는 동물의 사료로써 충분히 활용될 수 있을 것으로 기대된다.

• Mycobiology
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• 제45권4호
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• pp.286-296
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• 2017

Ulleung Island is a biodiversity hotspot in South Korea. During a survey of indigenous fungal species from Ulleung Island conducted from 2015 to 2016, we discovered 10 unrecorded macrofungi in Korea. These macrofungi were identified to the species level using morphological features and phylogenetic analysis based on the internal transcribed spacer region: Deconica phyllogena, Mycena zephirus, Phaeomarasmius proximans, Phlebia radiata, Pluteus semibulbosus, Postia alni, Resinicium pinicola, Scytinostroma portentosum, Tricholomopsis flammula, and Tyromyces kmetii. We also provide detailed morphological descriptions for these 10 species.