• Food Science of Animal Resources
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• v.37 no.2
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• pp.305-312
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• 2017

This study was conducted to establish the shelf-life of a milk beverage product supplemented with coffee extracts. Qualitative changes including peroxide value (PV), microorganism content, caffeine content, and sensory evaluation were measured periodically in beverages kept at 10, 20, and $30^{\circ}C$ for 8 wk. Lipid oxidation of the product was measured by peroxide value analysis, and apparent changes were observed during a 4 wk storage period. Caffeine analysis revealed that the changes in caffeine content were negligible during the storage period. Total aerobic bacteria, Escherichia coli, yeast, and mold were not detected in the products during an 8 wk storage period. Sensory evaluation revealed that after 4 wk of storage overall acceptance was less than 3 points on a 5-point scale. In this study, PV was used as an indicator of the shelf-life of the milk beverage product. PV analysis revealed that a value of 20 meq/kg was the end of the shelf-life using the Arrhenius equation and the accelerated shelf-life test (ASLT). Assuming that the beverages are kept at $4^{\circ}C$ during distribution, calculation of when the PV reached the quality limit point (20 meq/kg) was done with the equation ln(PV) = 0.3644X - 2.21834 and, using that equation, $PV=e^{0.3644X-2.21834}$ was calculated. Therefore, 14.3086 wk was determined to be the shelf-life of the milk beverage supplemented with coffee when stored at $4^{\circ}C$.

• Journal of the Korean GEO-environmental Society
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• v.15 no.8
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• pp.5-11
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• 2014

In this study, chemical analysis and ecotoxicity tests of leachate from disposal site for foot-and-mouth disease carcasses (FMD leachate) were conducted to collect fundamental data that will be used to develop environmental risk assessment tools for FMD leachate. For chemical analysis, concentration of $Cl^-$, $NH{_4}{^+}-N$, Korea standard method indicators for detection of leachate released from animal carcasses burial site into groundwater and NRN (Ninhydrin-Reactive Nitrogens), a newly suggested screening test indicator to detect groundwater contamination by FMD leachate, were assessed. For ecotoxicity tests, luminescent bacteria (V. fischeri), micro-algae (P. subcapitata) and water flea (D. magna) were selected as test species. Correlation analysis between the concentration of $Cl^-$, $NH{_4}{^+}-N$, NRN and the toxicity to V. fischeri was performed to identify the better indicators to monitor FMD leachate contamination. From regression analysis, the concentration of the indicators in FMD leachate contaminated sample that induced halfmaximal toxic effect to V. fischeri was evaluated. Results obtained from this study can be applied to assess the risk by FMD leachate and to establish the guideline to manage risk in relation to FMD leachate.

• Journal of the korean academy of Pediatric Dentistry
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• v.42 no.1
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• pp.30-37
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• 2015

A new colorimetric test ($Cariview^{(R)}$) using a new type of pH indicator can reflect the acidogenic potential of plaque bacteria. The objective of this study was to evaluate the correlation between $Cariview^{(R)}$ and the caries experience (measured through the dmft index) of children, and to compare $Cariview^{(R)}$ with Dentocult $SM^{(R)}$. Having obtained informed consent, 135 children less than 6 years old participated in the study. We examined their dmft index, and performed two caries activity tests ($Cariview^{(R)}$ and Dentocult $SM^{(R)}$) according to the manufacturers' instructions. In the results, $Cariview^{(R)}$ showed a moderate correlation with the dmft index (r = 0.43, p < 0.01). $Cariview^{(R)}$ showed a sensitivity of 68.8%, a specificity of 69.2%, and an area under curve of 0.686 in the ROC curve analysis. $Cariview^{(R)}$ had a significant correlation with the children's caries experience and had a slightly better explanatory ability than Dentocult $SM^{(R)}$. Furthermore, $Cariview^{(R)}$ was convenient and easy to use on uncooperative children, and also had an educational effect with its visual colors. It is suggested that $Cariview^{(R)}$ could be used clinically to identify the children susceptible to develop caries and to establish a preventive strategy.

• Korean Journal of Food Science and Technology
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• v.25 no.2
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• pp.174-177
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• 1993

Ultara violet ray air cleaner to use as the sterilization and deodorization device in refrigerator was designed and made of UV lamp and carbon block as the main components. The intensity of the lamp was $2.38\;mW/cm^2$ and reflector was installed to increase the radation. After running the device for 12 hours, 80% of the population of airborn bacteria was sterilized both at 48 and 480 liter refrigerator chambers. Ozone production caused by UV-ray reached 0.082 ppm at holding section within a few second when the device was operated at $25^{\circ}C$ and it showed 0.06 pm at $3^{\circ}C$. Deodorization effect was 2.5 times greater than that of a device made of electrical are principle at $25^{\circ}C$. New device 2 times more effectivly decreased trimethylamine and methyl mercaptan content applied as odor indicator substance in chamber at $3^{\circ}C$ than electrical arc type.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.2
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• pp.160-167
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• 2013

The influences of inland pollution sources nearJeokyang and Jangpoports following rainfall events on the bacteriological water quality and safety of commercial shellfish were investigated in the Changseon area, Korea. Stream flow rates exhibited 1.9- to 5.7-fold increases after rainfall events and then fell to 68~81% of that level after 24 h. The calculated impact area of inland pollution sources was 0.47 $km^2$ in the Jeokyangport area and 0.27 $km^2$ in the Jangpoport area at 24 h following 11 mm of rainfall. When the flow rate of inland pollution increased, the level of male-specific bacteriophage (MSB) decreased, and no MSB could be detected in seawater samples, whereas 30 PFU/100 g was detected in shellfish samples. Fecal coliform levels in seawater and shellfish samples did not exceed their respective tolerance levels (4.5 MPN/100 mL) and 130 MPN/100 g, respectively and thus complied with the standard for approved shellfish growing area. The sanitary conditions of areas adjacent to Jeokyang and Jangpoports under conditions of rainfall below 29 mm met the criteria for approved growing area under the United States National Shellfish Sanitation Program and the EC Regulations.

• Korean journal of food and cookery science
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• v.21 no.4 s.88
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• pp.440-446
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• 2005

To determine the shelf-life of low salted Myungran-jeot (low salted and fermented Alaska pollack roe), various chemical, microbiological and sensory analyses were carried out with Myungran-jeot stored at 10 days. The lactic acid content was slowly increased in the early stage of storage but then was rapidly increased with lengthening storage period, while pH was decreased during storage. The $NH_2-N$ content was steady at 10 days and peaked at 17 days. The production of VBN was increased to over $30mg\%$ at 19 days. In microbiological tests, the viable cell count and halotolerant bacteria were $1.5\times10^3\;and\;5.5\times10^3CFU/g$, respectively, at the initial state, but they slowly increased to $10^7 CFU/g$ at 17 days. Yeast or fungi did not appear on the surface of Myungran-jeot. Sensory evaluation showed that the original torture of Myungran-jeot in the mouth was a very important indicator for acceptability. Sensory characters and acceptability of Myungran-jeot at 17 days were not significantly different from those of the initial state. From these results, the estimated shelf-life of Myungran-jeot whom sealed with aluminum coated paper, was about 18 days in this study.

• Journal of dental hygiene science
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• v.8 no.4
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• pp.367-373
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• 2008

The natural products are used to be development of new antibacterial substances against human pathogenic bacteria. Adherence to the tooth surface by S. mutans is an important step in initiation of dental caries. This study was to examine antibacterial activity and anti-adhesive effect of Scutellaria baicalensis extract against S. mutans. Extracts of S. baicalensis were tested for antimicrobial activities by paper disc methods and radial diffusion assay methods, and bacterial adherence assay using 3 type of hydroxyapatite. The antibacterial level of ethyl acetate extract, IPK-3 on the growth of S. mutans was 125 mg/ml of minimum inhibitory concentration (MIC). The maximum growth of S. mutans in medium added with IPK-3 extract (50 mg/ml) was delayed to 30 hr, while the highest at 24 hr in control medium. The pH values of the control medium was 5.63 at 18 hr, but the media supplemented with IPK-3 extract was pH 6.50 at 12 hr. In adhesive inhibition assay, S. mutans was labelled with the fluorescent indicator DAPI and measured with fluorescence microscope. Adhesion of S. mutans on hydroxyapatite beads was inhibited by IPK-3 extracts. These results suggest that S. baicalensis extract can be used as an effective material for antibacterial activity and adhesive inhibition against S. mutans.

• Journal of Life Science
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• v.28 no.10
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• pp.1244-1253
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• 2018

Rumen microbiome consists of a wide variety of microorganisms, such as bacteria, archaea, protozoa, fungi, and viruses, that are in a symbiotic relationship in a strict anaerobic environment in the rumen. These rumen microbiome, a vital maker, play a significant role in feed fermentation within the rumen and produce different volatile fatty acids (VFAs). VFAs are essential for energy metabolism and protein synthesis of the host animal, even though emission of methane gas after feed fermentation is considered a negative indicator of loss of dietary energy of the host animal. To improve rumen microbial efficiency, a variety of approaches, such as feed formulation, the addition of natural feed additives, dietary feed-microbes, etc., have taken to increase ruminant performance. Recently with the application of high-throughput sequencing or next-generation sequencing technologies, especially for metagenomics and metatranscriptomics of rumen microbiomes, our understanding of rumen microbial diversity and function has significantly increased. The metaproteome and metabolome provide deeper insights into the complicated microbial network of the rumen ecosystem and its response to different ruminant diets to improve efficiency in animal production. This review summarized some recent advances of rumen microbiome techniques, especially "meta-omics," viz. metagenomic, metatranscriptomic, metaproteomic, and metabolomic techniques to increase feed fermentation and utilization in ruminants.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.5
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• pp.1435-1443
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• 2016

This study evaluated the sanitary state of seawater and shellfish in Yeoja bay from January 2014 to June 2016. The sampling stations for sanitary survey in Yeoja Bay was composed of 50 stations for seawater and 4 stations for ark shell and the samples have been collected monthly at a stated date. The total and fecal coliforms in seawater and Escherichia coli (E. coli) in shellfish were investigated as sanitary indicator bacteria, respectively. The geometric mean and the estimated 90th percentile ranges of total and fecal coliforms for seawater were <1.8-3.9, <1.8-2.6 and <1.8-29.7, <1.8-9.3 MPN/100 mL, respectively. The range of E. coli levels for ark shell were <20~330 MPN/100 g. The sanitary state in Yeoja bay was evaluated clean area according Korea criteria and conditionally approved area according US criteria and Class B area according EU criteria.

• Journal of Life Science
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• v.7 no.4
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• pp.316-321
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• 1997

The effects of the silver ion-exchanged water treatment agent (Ag-Os) upon E. coli RB 797 and Bacillus sp. have been discussed in this study. Silver ion causes a number of toxic effects with no known biological function. Silver ion-exchanged water treatment agent (Ag-Os) using oyster shell here showed antimicrobial activities. the soluble form of silver ion in water is more toxic to the growth of Bacillus sp. than that of E. Coli RB 797. The minium amount of Ag-Os needed for growth inhibition is 0.2 mg/ml for E. Coli RB 797 and 0.02 mg/ml for Bacillus sp., which is consistant with the data of the survival cell fraction. Binding studies suggested that binding of silver to the cell surface was a rapid, metabolic-independent process and different from active transport. Bacillus sp. showed more binding than E. Coli RB 797. Reducing substances of the cell cultures in the presence of Ag-Os was detected using Methylen blue as an indicator. From these results, we suggest that Ag-Os is effective as an antimicrobial agent on E. Coli RB 797 and Bacillus sp. and silver binds to the cells through rapid, metabolic-independent process and might complex to sulfur group in the cells for its toxicity.