• Journal of Environmental Health Sciences
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• v.47 no.2
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• pp.182-191
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• 2021

Objective: This study was performed to detect indicator bacteria in drinking spring water samples in Gwangju City and to identify their genus using the VITEK-II system. Methods: The subjects were ten drinking spring water sites in Gwangju. Samples of spring water were taken every month from September 2019 to August 2020. We analyzed for the indicator bacteria Yersinia and microorganisms isolated from the spring water. Result: According to the research results on indicator bacteria, general bacteria in st1-st7 with sterilization facilities in the spring and summer were investigated in the range of 0-2 CFU/mL and 0-12 CFU/mL. In st9, where a sterilization facility was not installed, the most general bacteria were detected (160 CFU/mL). Total coliform and fecal coliform showed unsatisfied rates of 16.7 and 11.1% in spring and 14.7 and 11.8% in summer, respectively. The unsatisfied rates of total coliform for the designated and non-designated spring water facilities were 3.8 and 47.1%, respectively, and for the fecal coliform group they were 2.5 and 35.3%. The difference was confirmed according to the presence of a sterilization facility. Yersinia spp. was not detected in all drinking spring water. Forty-one strains in 25 species were isolated from ten sites. The results classified as major dominant species are Pseudomonas spp. 14.6%, Pantoea spp. 9.8%, Serratia spp. 9.8%, Acinetobacter spp. 9.8%, Citrobacter spp. 7.3%, Bordetella spp. 7.3%, Delftia spp. 4.9%, and Enterobacter spp. 4.9%. Conclusions: Based on the result that various species derived from fecal pollution and artificial pollutants were detected in the non-specified public spring water facilities that many people use, the facilities need institutional complements such as continuous management or complete shutdowns.

• Journal of the Korean Institute of Landscape Architecture
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• v.28 no.5
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• pp.87-92
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• 2000

The purpose of this study is to investigate Ozone which we have to contact in our daily lives. Recently Ozone becomes a serious pollutant in the inside and outside. To select th indicator plants or the purifier plants for air pollution caused by ozone, this stud has been conducted with interior landscape plant of 10 species. The results are as follows; 1) The visible demage by Ozone was firstly observed on Viburnum awabuki among 10 species, and it occurred after the lapse of 10, 8 and 4 hours on 0.5, 1.0 and 2.0 ppm ozone treatment, respectively. The first symptom of visible demage appeared on chlorosis or blotting of leaves, then progressed on necrosis of leaves. 2) On the other hand, Litsea japonica and Ardisia japonica were so resistant against Ozone fumigation that they were not demage independently of Ozone concentration till the lapse of 60 hours. 3) Chlorophyll contents were decreased by the increased Ozone concentration. At this time, grana destruction was observed. The most sensitive plant to ozone was Viburnum awabuki, and we suggest that Viburnum awabuki would be possible one to use for the indicator plant. The most resistance plant to Ozone was Litsea japonic and Ardisia japonica, we suggest that Litsea japonica and Ardisia japonica would be possible to use for the purifier plant for Ozone pollution.

• BMB Reports
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• v.46 no.12
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• pp.600-605
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• 2013

Interplay between calcium ions ($Ca^{2+}$) and reactive oxygen species (ROS) delicately controls diverse pathophysiological functions of vascular smooth muscle cells (VSMCs). However, details of the $Ca^{2+}$ and ROS signaling network have been hindered by the absence of a method for dual measurement of $Ca^{2+}$ and ROS. Here, a real-time monitoring system for $Ca^{2+}$ and ROS was established using a genetically encoded hydrogen peroxide indicator, HyPer, and a ratiometric $Ca^{2+}$ indicator, fura-2. For the simultaneous detection of fura-2 and HyPer signals, 540 nm emission filter and 500 nm~ dichroic beamsplitter were combined with conventional exciters. The wide excitation spectrum of HyPer resulted in marginal cross-contamination with fura-2 signal. However, physiological $Ca^{2+}$ transient and hydrogen peroxide were practically measurable in HyPer-expressing, fura-2-loaded VSMCs. Indeed, distinct $Ca^{2+}$ and ROS signals could be successfully detected in serotonin-stimulated VSMCs. The system established in this study is applicable to studies of crosstalk between $Ca^{2+}$ and ROS.

• Journal of the Korean Institute of Landscape Architecture
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• v.38 no.4
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• pp.106-127
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• 2010

Although multi-layer planting methods are more widely used as a method for clustered planting and environmental programs such as plant remediation, difficulties have been faced in applying those to planting design. This study develops a basic planting model that can be applied to multi-layer planting in basis on an analysis of forest structures in the Seoul area. An optimal number of clusters was determined through the ISA (Indicator Species Analysis), and 7 basic clusters were found through a cluster analysis by using PC ORD 4.0 software specifically developed for ecological analysis. The 7 basic clusters include the following communities: the Quercus acutissima Community, Sorbus alnifolia-Quercus mongolica Community, Pinus rigida-Pinus densifiora Community, Rododendron mucronulatum var. mucronulatum-Quercus mongolica Community, Juniperus rigida-Quercus mongolica Community, Rododendron mucronulatum var. mucronulatum-Pinus densiflora Community, and Rododendron sclippenbachii-Quercus mongolica Community. The study also selected 57 species with at least a 10% frequency among the plant species existing in the Seoul area and suggested both a companion species and available similar alternative species by conducting an additional interspecific association analysis. This study may help to enhance usefulness of the model in architectural planting design. In addition, the two results named above were synthesized to develop a multi-layer planting model that can be utilized in landscape planting design by selecting similar alternative species through the interspecific association analysis, which includes 7 clusters of natural plants. The multi-layer planting model can be widely applied to design planting because the model has an average target cover range based on the average value of a transformed likelihood.

• Korean Journal of Plant Taxonomy
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• v.51 no.1
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• pp.49-99
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• 2021

Gwangdeoksan Mountain (699.3 m) is the highest border mountain between the two cities of Chungcheongnamdo Province, Cheonan and Asan, Korea. In this study, we investigated the flora of Gwangdeoksan Mt. from April of 2015 to October of 2017. Through 20 independent field investigations, we identified and tallied a total of 428 species, 9 subspecies, 30 varieties, and a forma in 287 genera and 97 families. Of a total of 468 taxa, 128 taxa in 112 genera and 58 families were found to be Korean endemic species (7 taxa), floristic regional indicator species (45 taxa), rare or endangered species (3 taxa), species subject to the approval of outbound transfer (73 taxa), and alien or ecosystem disturbing species (32 taxa). The flora of Gwangdeoksan Mt. can be divided into four distinct floristic subregions, with higher diversity in the north-facing subregion. The complex flora of Gwangdeoksan Mt., emerging at the edge of two floristic regions of the Korean peninsula, may represent a significant conservation priority and a topic for future ecological and geographical studies.

• Journal of Korean Society of Forest Science
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• v.99 no.5
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• pp.770-776
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• 2010

This study was conducted to assess the existing flora and fauna, and to develop a spatial map of Alapang communal forest located in the province of Benguet, Philippines. A total of 52 species belonging to 27 families were identified during the inventory in this communal forest using the quadrat method while a total of 30 species belonging to 18 families were recorded using line intercept technique for the assessment of grasses, herbs, vines and other low-lying vegetation. The diversity index of the species in Alapang communal forests using the quadrat method was 2.6649 while for the line intercept technique it was 2.5446. The most dominant species in this area was found to be Pinus kesiya Royle ex Gordon (Benguet pine) under Family Pinaceae with an importance value of 106.74%. In the faunal assessment, four species of birds and a small mammal particularly a rodent were identified during the study. Aside from the high species diversity of this communal forest, the presence of endemic and indicator species in the area denotes that this forest was still in good condition hence must be protected. Spatial maps and database system were generated based from data gathered in the field using Geographic Information System (GIS).

• The Korean Journal of Soil Zoology
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• v.3 no.2
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• pp.91-105
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• 1998

This study was conducted to analyze oribatid mites communities in deciduous forests in Namsan and Kwangreung on which different levels of environmental stresses might press, and to select potential condidate of biological indicator species. In overall, pH was lower in soil than in litter layer. Also soil pH, total nitrate content and soluble sulfate content were significantly higher in Namsan than in Kwangreung (p<0.05). The flora in Namsan and Kwangreung deciduous forests were dominated by Quercus mongolica, and Carpinus laxiflora respectively. It indicated that Namsan was in the lower stage of the floral succession. The number of species of oribatid mites was significantly higher in Kwangreung (39 families, 65 genera and 114 species) than in Namsan (32 families, 53 genera and 89 species) (p<0.05). Common species found in two regions were 77 species. Mean density of oribatid mites was 1.8 times higher in Kwangreung than in Namsan and this difference came mainly from litter layer. Among correlation analyses between environmental factors and oribatid mites density and species number, only density was negatively correlated with fresh litter depth in Namsan litter layer (r=-0.89, p<0.05). The pH which was considered to affect distribution of oribatid mites didn't show significant results.

• Journal of Environmental Science International
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• v.19 no.12
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• pp.1385-1395
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• 2010

Harmful dinoflagellate Cochlodinium polykrikoides blooms have been frequently occurred in coastal areas of the East Sea since 1995. We compared the oceanic conditions in years 1995, 2001 and 2003 when the C. polykrikoides bloom was strong, and in years 1998 and 2004 when the C. polykrikoides bloom was not appeared. We studied temporal and spatial variation of upwelling and geostrophic currents on the western channel of Korean Strait, an entrance of the East Sea. The period and occurrence area of C. polykrikoides bloom was depended on variation of upwelling in summer. In the distributions of geostrophic current, southward current was dominant near the coast in August, 1998 and 2000. Whereas northward current was dominant near and off the coast in August, 1995 and 2003 which the C. polykrikoides bloom was strong. When compared dominant phytoplankton of the coastal areas in each year, Kuroshio indicator species Proboscia alata and Chaetoceros affine were dominant, respectively, in 2001 and 2003 at every stations. However, the dominant species was variable at each coastal area in 1998 and 2000. In 2003, the abundance of Sagitta elegans which is known as the cold water indicator was low, but the abundance of S. enflata, warm water indicator, was very high in Gangneung compared to Sokcho. It seemed that the distribution of S. elegans is restricted by strong warm water current. In conclusion, it was estimated that the distribution of C. polykrikoides bloom in the coastal area of the East Sea was closely related with the strength of East Korea Warm Current and upwelling.

• Journal of Environmental Health Sciences
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• v.24 no.1
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• pp.141-150
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• 1998

This study was performed to investigate the distribution of Yersiniae and correlation between Yersiniae and indicator organism by time and area in spring water located in northern part of Seoul. Samples collected from 46 spring waters located in four mountains(Dobong, Bukhan, Surak, Bulam) were inspected to detect Yersiniae and indicator organisms. And also there were examined bioserological characteristics and resistance of ahtibiotics of the isolated Yersiniae.The result were as follows. 1. The isolation rate of Yersiniae was 22% in February and 20% in April. The isolated species were 6 strains of Y. enterocolitica, 6 strains of Y. aldova, 4 strains of Y. intermedia and 43 strains of Y. pseudotuberculosis. The serotype of Y. pseudotuberculosis isolated from was all O:5 and biotype of Y. enterocolitica isolated from was all O:3. 2. The Geometric mean of standard plate count, coliform, and psychrotrophilic bacteria were 3.4 CFU/ml, 1.2 MPN/100 ml and 33.0 CFU/ml in February and 3.1 CFU/ml, 1.5 MPN/100 ml and 20.5 CFU/ml in April respectively. There was no significant difference by time and area but the indicator organisms were correlated significantly with each other (p<0.05). 3. Because detection of Yersiniae was not statistically associated with indicator organism, Yersiniae can be detected in the spring water approved microbiologically (p<0.05). 4. The Yersiniae isolated were resistant to Ampicillin, Colistin, Carbenicillin and Coilstin. All isolaed Y. enterocolitica were resistant to Ampicillin (100%). In the case of Y. pseudotuberculosis, only 1 of 3 isolated was resistant to Colistin but susceptible to other antibiotics.

• Korean Journal of Ecology and Environment
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• v.53 no.3
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• pp.286-294
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• 2020

The Chironomus(Diptera: Chironomidae) is a freshwater benthic invertebrate that is an important indicator organism used for environmental pollution and water quality monitoring. In this study, we performed morphological classification and genetic species identification using the cytochrome c oxidase subunit I (COI) gene on mitochondrial DNA for an accurate species classification of Chironomus larvae found in tap water purification plants in Incheon, Korea. Twenty larvae in six water purification plants consist of four species, including twelve Chironomus kiiensis, six Chironomus flaviplumus, one Chironomus dorsalis, and one Polypedilum yongsanensis (not included Genus Chironomus). Morphological characteristics of each larvae were identified based on the head capsule, the mentum, the mandible, the antenna, and the claw. Based on the COI sequences of 21 individuals of 17 Chironomus species registered in NCBI Genbank, phylogenetic analysis indicated that the 20 individuals investigated in this study consist of the same clade with corresponding species of the high homology (99~100%) including C. kiiensis, C. flaviplumus, C. dorsalis, and P. yongsanensis. These results will be used as main classification indicator for monitoring freshwater ecosystems by providing integrated morphological and genetic information for the species identification of Korean Chironomus.