• BMB Reports
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• 제29권1호
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• pp.63-67
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• 1996

In order to elucidate the molecular mechanism of plasmid incompatibility of broad host-range plasmid R1162, the plasmid-encoded replication protein RepIB was purified and tested for binding to the 20 bp direct repeat (DR) DNA sequence which is reiterated 3 and 1/2 times within the replicative origin of the plasmid. The RepIB protein specifically binds to the DR DNA. Point mutations in the DR which affect expression of plasmid incompatibility also coordinately affect binding. These results indicate that the incompatibility of broad host-range plasmid R1162 is exerted by the DR DNA by titrating the essential replication protein RepIB.

• Journal of Microbiology and Biotechnology
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• 제25권10호
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• pp.1614-1620
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• 2015

Plasmid-cured derivative strains of Bacillus anthracis are frequently used in laboratory studies. Plasmid incompatibility, which does not increase the risk of chromosomal mutation, is a useful method for plasmid curing. However, in bacteria containing multiple plasmids, it often requires the sequential introduction of multiple, specific incompatibility plasmids. This lengthy process renders the traditional plasmid incompatibility method inefficient and mutation-prone. In this study, we successfully cured plasmids pXO1 and pXO2 from B. anthracis A16 simultaneously using only one recombinant incompatible plasmid, pKORT, to obtain a plasmid-free strain, designated A16DD. This method may also be useful for the simultaneous, one-step curing of multiple plasmids from other bacteria, including Bacillus thuringiensis and Yersinia pestis.

• 한국응용곤충학회지
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• 제46권1호
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• pp.57-62
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• 2007

자생밀도의 증가를 위해 도입되는 생물적 방제인자를 선정하는 데 있어서 생식형불일치는 주요한 요인이다. 배추좀나방(Plutella xylostella(L.))에 대한 효과적 생물적방제 인자로서 프루텔고치벌(Cotesia plutellae (Kurdjumov))은 집단 사이에 생식형불일치로 크게 두 생식형집단으로 분류되었다. 본 연구는 국내 서식하는 특정 프루텔고치벌 집단에 대해서 형태적 및 미토콘드리아 염기서열 지표분석을 실시하였으며, 이 결과 국내 집단이 기존에 보고된 두 생식형집단에 비해 형태적 형질과 분자지표에서 뚜렷한 차이를 보였다. 이러한 결과는 국내 프루텔고치벌 집단이 새로운 생식형집단일 수 있다는 것을 제시하였다. 또한 본 연구 결과는 외래 프루텔고치벌의 도입을 위해서는 국내 자생종과의 생식형불일치 가능성을 중요하게 고려해야 한다고 보고한다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.191-191
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• 2022

Heterostyly continues to fascinate evolutionary biologists interested in heredity, evolution, breeding, and adaptive function. Polymorphism demonstrates how simply inherited developmental changes in the location of plant sexual associations can have important consequences for population pollination and mating biology. In contrast to homozygous self incompatibility, only a small number of mating phenotypes can be maintained in the population because insect pollinators have limitations in achieving multiple segregation sites for pollen deposition. Field studies of pollen tube growth have shown that reciprocal style-stamen polymorphisms function to increase the capacity of insect-mediated cross-pollination. The genetic pattern of style morphs is well established in various taxa, but despite recent advances, the identity, number, and structure of the genes controlling the heteromorphic syndrome have been poorly elucidated. The phenomenon of heterostyly in buckwheat has been controlled by gene complex concentrate to S-locus. Homomorphic autogamous buckwheat strains were established by the interspecific hybridization. Backcrossing of this line to the common buckwheat (pin) and selecting homostylar progenies made it possible to introduce the self-compatible gene into common buckwheat. In the result, we obtained the BC₉F₂ generation, and defined the strong linkage between flower type and self-incompatibility by microscopic observation of pollen tube growth. This finding suggests that self-incompatibility character is not controlled by one gene. Moreover, we defined the strong linkage between flower type and self-incompatibility. It strongly supports the S supergene theory. Therefore, we have plan to elucidate the heterostyly self-incompatibility by using molecular genetics, proteome analysis and apply to exploitation of buckwheat improvement. In near future, the expression of heterozygous syndromes in genus Fagopyrum with single isolated heterozygous species may provide clues to early stages of polymorphic assembly and shed light on evolutionary models of heterozygous strains.

• 한국식품과학회지
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• 제30권5호
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• pp.1035-1039
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• 1998

수용액상에서 단백질과 다당류 사이의 비혼합성은 일반적인 현상이므로 다양한 조성과 형태의 수용성 거대분자를 포함하는 식품에서 상분리는 매우 전형적인 현상이다. 본 연구에서는 식품원료로 자주 쓰이는 카제인과 알긴산을 이용하여, pH (6, 8, 10), NaCl 농도(0, 0.25, 0.5 M), 알긴산의 분자량$(1.64{\times}10^5,\;1.95{\times}10^5)$에 따른 상분리현상에 대하여 연구하였다. 식품에서 카제인이 유화제로 작용할 때 알긴산의 첨가에 따른 혼합성의 변화는 카제인의 유화안정성에 영향을 끼치게 된다. 알긴산의 분자량에 따른 혼합성의 차이는 크게 나타나지 않았다. pH가 높을수록, 염농도가 낮을수록 카제인-알긴산 혼합물의 비혼합성은 감소하였다. 이 결과로 보아 카제인과 알긴산 사이의 정전기적 작용이 혼합성에 관련되어 있는 것으로 생각된다.

• 생명과학회지
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• 제19권11호
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• pp.1666-1671
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• 2009

배추과에 있어서 SLG (S locus glycoprotein)와 SRK (S locus receptor kinase) 유전자는 자가불화합성의 인식반응에 관여한다. 또한 수화작용에 중요한 aquaporin (MOD) 유전자 역시 자가불화합성 작용에 요구된다. 본 실험은 양배추 자가화 합성(SC) 계통으로부터 SC-SLG, SC-SRK, 및 SC-MOD 유전자들을 동정하고 이들 유전자의 물리적 구조를 분석하였다. 그 결과 이들 유전자들은 기존에 보고된 유전자들과 높은 상동성을 보였고 RT-PCR에 의한 발현 해석에 있어서 정상적인 발현을 나타내었다. 이들 결과들로 부터, 자가화합 양배추 계통은 주두측 자가불화합성 유전자에 이상이 있는 것이 아니라 화분측 자가불화합성 유전자의 이상 그리고/또는 S-locus에 연쇄 되어 있지 않지만 자가불화합성에 관여하는 유전자의 변이에 의해 자가불화합성이 타파되었다고 생각된다.

• The Plant Pathology Journal
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• 제21권2호
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• pp.106-110
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• 2005

Sclerotinia sclerotiorum attacks most of the vegetable crops in the Jordan valley. Twenty-five samples/isolates were obtained in a complete coverage of that region. They were characterized for their mycelium incompatibility, and specific gene amplified using the primer SSREV/SSFWD. All isolates gave similar single band around 278 bp. Thirteen isolates were completely incompatible with the other 12 ones. The latter ones fell into four subgroups of mycelium incompatibility. RAPD analysis using three primers (OPA-2, OPA-10, and OPA-18) clustered the 25 isolates into subgroups in agreement with their morphological separation, indicating close correlation between amplified gene(s) and the gene(s) of incompatibility. All highly virulent isolates were among the group of 13, indicating a well established genomic type pathogen in this region.

• BMB Reports
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• 제30권1호
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• pp.18-20
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• 1997

Lycopersicon peruvianum shows a gametophytic self-incompatibility (GSI). GSI is controlled by a single locus (S locus) with multiple alleles. S ribonucleases encoded in S alleles cosegregate with their phenotypes of GSI in genetic cross. To understand the genetic role of S allele in L peruvianum, two large genomic fragments isolated previously were analyzed with total genomic DNAs from several tomato lines generated by cross-pollination. Southern blot analysis with the S allele fragments as probes revealed that the flanking region of S allele contained the highly homologous regions. It is speculated that they may play an important role to prevent genetic cross by self-pollination.

• Interaction and multiscale mechanics
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• 제1권4호
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• pp.423-435
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• 2008

This paper employs the reproducing kernel (RK) approximation for evaluation of field theory-based incompatibility tensor in a polycrystalline plasticity simulation. The modulation patterns, which is interpreted as mimicking geometrical-type dislocation substructures, are obtained based on the proposed method. Comparisons are made using FEM and RK based approximation methods among different support sizes and other evaluation conditions of the strain gradients. It is demonstrated that the evolution of the modulation patterns needs to be accurately calculated at each time step to yield a correct physical interpretation. The effect of the higher order strain derivative processing zone on the predicted modulation patterns is also discussed.

• 가정과삶의질연구
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• 제8권1호
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• pp.49-67
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• 1990

The purpose of this study is to investigate the relations between marital status incompatibility and the ways of dealing with marital conflict. Research was conducted by interview using questionnaire on 177 employed wives. Statistics used for data analysis were frequency distribution, percentage, t-test, chi-square test. 1) In resolving marital conflict four types of method are appeared, namely constructive method, passive method, evasion and physical violence. 2) Only physical violence of husband-to-wife of the four methods is related to status in-compatibility. That is the incidence rate of physical violence of husband-to-wife in incompatible couples is higher than that of compatible couples in conflict situations. 3) In case the wife's occupational status is high relative to her husband the incidence rate of physical violence of husband-to-wife is extremely high, but is significantly low in couples which are compatible in this status. 4) In case wife's status advance ent is controlled, there is significant relationship between status incompatibility and the method of conflict resolution by husband whose wife is status striver.