• 대한본초학회지
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• 제26권4호
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• pp.125-132
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• 2011

Objectives : An aim of study is to investigate effects of curculiginis rhizoma in vitro (factor Xa (FXa) inhibitor assay, prothrombinase assay, prothrombin time (PT) assay, activated partial thromboplastin time (aPTT) assay) and in vivo experiment (blood clotting time, thromboxane B2 content assay in serum and weight of thrombus by AV-shunt rat model). Methods : We gained a human serum and used serum in vitro study such as factor X activity (FXa) inhibition, prothrombinase inhibition, prothrombin time (PT) and activated partial thromboplastin time. Fifteen SD rats were divided into three groups (intact control group and two experimental group treated with extract of Curculiginis Rhizoma(ECR)). Rats were orally administrated DW (intact control group), 600 mg/kg concertration of ECR and 200 mg/kg concertration of ECR. After one hour, we anesthetized rats and made arteriovenous (AV) shunt rat models to study weights of thrombus, took a hole blood to study content of thromboxane B2 and blood clotting time. Results : In vitro, ECR increased a inhibitory activity of FXa, prothrombinase and aPTT compared than intact control group. Especially ECR made significant increase of FXa and prothrombinase inhibitory activity (p<0.05, p<0.01). And PT were increased in ECR control group compared with intact control group. In vivo, a blood clotting time of experiment group treated with ECR 600 mg/kg were significantly increased compared with that of intact control group (p<0.05) and content of thromboxane B2 was significantly decreased in group treated with ECR 600 mg/kg in seum. The weight of thrombus were significantly reduced in group treated with ECR 600 mg/kg compared with intact control group (p<0.05). But in vivo experiment study, those of group treated with ECR 200 mg/kg were reduced compared with those of intact control group without statistical significance. Conclusions : ECR has a antithromboic activity in internal course with inhibitory activity of FXa and prothrombinase in vitro, it required to research more study for effective compounds.

• 대한가정학회지
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• 제24권1호
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• pp.53-58
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• 1986

In order to study the antioxidative action of the effectient garlic components, peroxidase and superoxide dismutase activity were compared through the in vitro and in vivo experiments. RESULTS : 1. Observing the effects on peroxidase activity of efficient components in vitro, garlic oil, alliin and ethanol fraction showed effective, which was similar to the trend of TBA value, peroxide value and induction time for the first period of lipoperoxide formation in vitro. 2. In vivo experiment with peroxidase activity, the ethanol fraction and garlic oil were effective when intraperitoneally administered as well as orally administered. 3. Considering the superoxide disutase activity in vitro, the garlic oil, alliin and ethanol fraction were effective in efficient components. But non-kaolin fraction inhibited the activity on the contrary. 4. In terms of the efefcts on superoxide dismutase activity in vivo, alliin and garlic oil wee effective in intraperitoneal adminstraton and the ethanol fraction and alliin in oral administration.

• 동의신경정신과학회지
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• 제17권1호
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• pp.79-105
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• 2006

Objective : This research aims to investigates the effects of CMT and HCMT on Dementia. and we also want to know the different effect of CMT, HCMT by drug types. Methods : The research is progressed by two types of experiment. one experiment is BV2 microglial cell line treated by LPS in vitro and another experiment is memory deficit mice induced by scopolamine in vivo. Results : The CMT and HCMT is effective in BV2 microglial cell line treated by LPS in vitro and in the serum of the memory deficit mice induced by scopolamine in vivo. But, there is no significant difference between CMT and HCMT extract&nano powder in experimental conclusion. Conclusions : These results suggest that the two drug types of CMT and HCMT may be effective for the prevention and treatment of Dementia. Investigation into the further study two drug types of the CMT and HCMT for Dementia is suggested for future research.

• Journal of Nutrition and Health
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• 제26권4호
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• pp.379-389
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• 1993

Several studies have shown that extracts from yellow-green vegetables reveal antitumor activities. In the present study we investigated the effect of phytol in order to elucidate the immunological mechanism of antitumor activity of this substance. The results obtained from the experiment as follows: 1) Phytol showed cytotoxic effect on sarcoma 180 cells in vitro. 2) When phytol was injected into the peritoneal cavity of mice transplanted with sarcoma 180 cells, the average survival time (24.0 days) tended to increase as compared with the nontreated control (19.2 days). 3) When sarcoma 180 cells were injected subcutaneously into the right groin of mice, and then phytol was injected into the peritoneal cavity, the tumor inhibition ratio was 33%. 4) The natural killer(NK) cell activity was significantly augmented by phytol in vitro and in vivo. Similar augmentations of NK cell activity were obtained with culture supernatants of phytol exposed spleen cells and peripheral blood mononuiclear cells. 5) Phytol on the macrophage from peritoneal cavity showed a higher effectiveness in vivo than in vitro. These results indicate that phytol shows the inhibitory effect for growth of sarcoma 180 cells in vitro, also it can augment macrophage and NK cell activities in vivo.

• Journal of Acupuncture Research
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• 제23권4호
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• pp.187-203
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• 2006

Objectives : This study was designed to investigate the antimetastatic and immunomodulating effects of Patriniae Radix. Methods : Acute and subacute cytotoxicity experiment of Patriniae Radix was performed. Antimetastatic experiment was administered in vitro and in vivo. To observe the immunomodulating effects of Patriniae Radix, FACS analysis and ELISA assay were performed. Results : There was no acute and subacute toxicity responses in mouse treated with Patriniae Radix. Antimetastatic experiment in vitro and in vivo showed that Patriniae Radix has antimetastatic effects. This research revealed that Patriniae Radix mediate cellular immunity response. As compared with control, the population of total T cell, helper T cell, cytotoxic T cell and macrophage were increased. The production of Th 1 type cytokines from splenocyte and cytokines which is associated with anti-tumor activity form macrophage were increased significantly. Conclusion Patriniae Radix Herbal-acupuncture appears to have considerable activity on the treatment of liver metastasis from colon26-L5 carcinoma cell line, and deserves further evaluation in this setting.

• 대한의생명과학회지
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• 제10권3호
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• pp.219-230
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• 2004

This experiment was performed to examine the in vitro and in vivo affects of the two different haplotype strains of mice, BALB/c and C3H/HeN infected with Echinostoma hortense, and the manifestation of the profiles of cytokine in the splenocytes. In the in vitro experiment, the two mice's splenocytes were divided and stimulated with antigen of crude extracts and the antigen of excretory and secretory products of an adult warm and the manifestation of cytokine mRNA was verified with RT-PCR. As a result, the two different strains of mice both strongly manifested the Th2 cytokine rather than the Thl cytokine and in the case of the Th2 cytokine, the BALB/c mice manifested more strongly than the C3H/HeN mice. In the experiment using the ELISA method, the protem cytokine manifestation had the same result as the mRNA experiment. In the in vivo experiment, the mice was infected via oral route with the metacercaria of the Echinostoma hortense and the manifestation of cytokine was verified by RT-PCR and ELISA and the results were the same as the in vitro experiment. Therefore, in the two strains of BALB/c and C3H/HeN, the C3H/HeN showed a higher susceptivity to the Echinostoma hortense.

• 한국전자파학회:학술대회논문집
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• 한국전자파학회 2003년도 종합학술발표회 논문집 Vol.13 No.1
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• pp.13-17
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• 2003

In this paper, the design parameters for the magnetic field source at extremely low frequency are proposed. This facility can be used for in vivo experiments with small animals to investigate biological response to the driving magnetic fields. In case that the exposed animals are motionless, the animals may be affected by the directivity of driving field. To avoid this effect, a 2-axis ELF magnetic field driving apparatus was designed, The optimum location and number of turns of each coil were obtained by numerical analysis. Applying these data to the MATLAB code (for computation), the magnetic field distribution was obtained. The calculation result for a well-designed facility showed that the space in which the amplitude of the magnetic field lies within the 95% of the magnetic field distribution was more than 60% of each axis length.

• 대한의용생체공학회:의공학회지
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• 제23권2호
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• pp.131-137
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• 2002

이동 작동기형 인공 심장의 원격 모니터링을 위한 시스템을 설계, 구현하였다 동물 실험이나 환자에의 임상 실험 후 인공 심장 동작 상황에 대한 원격 모니터링은 필수적이다. 본 논문에서는 담당자가 환자로부터 멀리 떨어진 곳에 있을 때에도 인터넷을 통해 간편하게 인공 심장의 동작 상황과 혈류역학 정보를 확인할 수 있는 시스템을 제안하여 그 성능을 확인하였다. 기존의 시스템과 쉽게 연결하여 사용할 수 있도록. 또 쉬운 관리와 기능 향상을 위하여 COM(Component Object Model) 기술 기반의 콤포넌트 모듈로 데이터 전송 부분을 구현하였고. 범용의 브라우저를 이용하여 인터넷에 연결, 확인토록 자바 애플릿을 이용하여 실행되도록 하였다. 또한 정해진 룰에 따라 판단하여 인공 심장의 이상 동작시 관리자에게 알려주도록 하였다. 구현된 시스템을 1개월 이상 생존하는 동물에 적용하여 본 결과 아무런 문제없이 정상 동작함을 확인하였다.

• 원예과학기술지
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• 제29권2호
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• pp.151-155
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• 2011

Botrytis cinerea에 의한 딸기 잿빛곰팡이병은 최대 50%까지 발생하여 큰 피해를 주는 딸기의 주요 병해이다. 약제살포시기는 잿빛곰팡이병의 성공적인 방제를 좌우하는 필수적 요소이다. 딸기 '설향' 품종에 대한 잿빛곰팡이병 방제효과 검정을 위해 fenhexamid + imonoctadine tris, cyprodinil + fludioxonil, Bacillus subtilis QST713을 ex vivo에서 개화전, 개화기, 미착색과, 성숙과 단계부터 처리하여 효과를 비교하였다. 또한 '설향' 품종을 공시하여 ex vivo와 재배포장에서 cyprodinil + fludioxonil 혹은 fludioxonil을 저온($0^{\circ}C$, 5시간) 전후 처리하여 잿빛곰팡이병 방제효과를 조사하였다. 딸기 꽃과 성숙과실은 미착색과실보다 B. cinerea에 더 감수성이었다. ex vivo에서 개화단계별 약제방제효과는 fenhexamid + iminoctadine tris는 개화직전과 개화기, cyprodinil + fludioxonil은 개화기에 효과적이었으나 Bacillus subtilis QST713은 처리간 유의성이 없었다. 2010년 딸기 잿빛곰팡이병 방제효과는 fludioxonil을 개화기에 1주일 간격으로 2회 처리시 효과적이었다. 딸기 'Redpearl'과 '설향' 품종은 '매향'과 'Akihime' 품종보다 저온에 더 감수성이어서 주의를 요했으며 cyprodinil + fludioxonil을 저온처리 전에 살포시 90%이상의 방제효과를 나타냈다. 2010년 재배포장에서 잿빛곰팡이병 방제효과는 저온 발생 전 혹은 저온 발생 후 1주일 간격 2-3회 fludioxonil처리가 효과적이었다. 이런 결과로 딸기 잿빛곰팡이병 방제를 위한 약제의 효과적인 처리시기는 개화와 저온이 밀접한 관련이 있음을 알 수 있었다.

• Journal of Pharmaceutical Investigation
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• 제29권2호
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• pp.137-143
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• 1999

Ketoprofen together with various permeation enhancers was incorporated into a novel soft hydrogel which is semi-solid in a container and to form a thin film within a few minutes after applying on the skin. The effect of various enhancers on the skin permeation of ketoprofen from a soft hydrogel was investigated using in vitro and in vivo method. In vitro rat skin permeation of ketoprofen from soft hydrogel was conducted using modified Keshary-Chien diffusion cells. In vivo ketoprofen absorption was also investigated in rats, and the results were compared with that of commercial products. Anti-inflammatory activities were determined using carrageenan-induced paw edema method and adjuvant-induced arthritis method in rats. The anti-inflammatory activity of ketoprofen soft hydrogel formulation with that of commercial products were compared. In vitro as well as in vivo studies showed that $HPE-101^{\circledR}$ was the most effective skin permeation enhancer among those used in this study. Addition of an adhesive (polyisobutylene) in the soft hydrogel decreased skin permeation of ketoprofen. Paw edema and anti-arthritis tests showed that soft hydrogel containing $HPE-101^{\circledR}$ was more effective than the commercial products, which was consistent with the in vivo absorption experiment results.