• Clinical and Experimental Reproductive Medicine
• /
• 제44권2호
• /
• pp.63-72
• /
• 2017

Objective: Hyperstimulation methods are broadly used for in vitro fertilization (IVF) in patients with infertility; however, the side effects associated with these therapies, such as ovarian hyperstimulation syndrome (OHSS), have not been well studied. N-glycoproteomes are subproteomes used for the remote sensing of ovarian stimulation in follicular growth. Glycoproteomic variation in human follicular fluid (hFF) has not been evaluated. In this study, we aimed to identify and quantify the glycoproteomes and N-glycoproteins (N-GPs) in natural and stimulated hFF using label-free nano-liquid chromatography/electrospray ionization-quad time-of-flight mass spectrometry. Methods: For profiling of the total proteome and glycoproteome, pooled protein samples from natural and stimulated hFF samples were selectively isolated using hydrazide chemistry to obtain the total proteomes and glycoproteomes. N-GPs were validated by the consensus sequence N-X-S/T (92.2% specificity for the N-glycomotif at p<0.05). All data were compared between natural versus hyperstimulated hFF samples. Results: We detected 41 and 44 N-GPs in the natural and stimulated hFF samples, respectively. Importantly, we identified 11 N-GPs with greater than two-fold upregulation in stimulated hFF samples compared to natural hFF samples. We also validated the novel N-GPs thyroxine-binding globulin, vitamin D-binding protein, and complement proteins C3 and C9. Conclusion: We identified and classified N-GPs in hFF to improve our understanding of follicular physiology in patients requiring assisted reproduction. Our results provided important insights into the prevention of hyperstimulation side effects, such as OHSS.

• 한국운동역학회지
• /
• 제13권3호
• /
• pp.253-264
• /
• 2003

This study aims at finding the structure of spike technique by analysing comparatively the spike action by excellent and by non-excellent players throughout the section from a flying jump to the time of landing for the correct analysis of spike action and tries to help athletes and coaches to execute a scientific training. For the objected person of this study, six of H College athletes three of excellent athletes and three of non-excellent athletes, presently registered as athlete with the Korea Volleyball federation) were chosen, and the factors of analysis were analysed upon performance time of action by section, human body centered displacement, change of articulation angle, speed change of articulation of the upper limbs, uniformity of the articulation of the upper limbs upon impact, etc. The conclusion of this study is as follow: 1. In the time required for taking action, it shows to take $1.067{\pm}0.057$ seconds for the group of excellent athletes and $1.034{\pm}0.033$ seconds for the group of non-excellent athletes. Although there was not big difference between two groups in the performance time of action, it showed that the group of excellent athletes takes longer compared to the group of non-excellent athletes. And it was found by the result of this study that the group of excellent athletes stays longer in the duration of flight. 2. In the displacements of horizontal movement and vertical movement, it was found that the group of excellent athletes have moved more than the group of non-excellent athletes in the horizontal movement of the center of human body 3. In the angles of wrist and knee, it was found that the excellent athletes have shown little than the non-excellent athletes in the entire sections, but that in the angle of elbow, the non-excellent athletes have shown bigger than the excellent athletes.. 4. In the speed of the articulation of the upper limbs upon impact, it was found that the group of excellent athletes have shown bigger than the group of non-excellent athletes, and that in the maximum value of the articulation of the upper limbs, the maximum value for the hand was indicated upon impact and that forearm and upper arm have shown the maximum value just before the impact. 5. In the uniformity of articulation of the upper limbs at the time of impact, the group of excellent athletes showed bigger than the group of non-excellent athletes in all the articulations.

• 한국대기환경학회지
• /
• 제33권2호
• /
• pp.124-138
• /
• 2017

Physico-chemical measurement of non-refractory submicron particles($NR-PM_1$) was conducted in Baengnyeong Island, Korea using Aerodyne High Resolution Time of Flight Aerosol Mass Spectrometer (HR-ToF-AMS) from 2012 to 2014. Organics and ammoniated sulfate were dominant species in $NR-PM_1$. The organics was found to have similar fractions(approximate 40%) of $NR-PM_1$ during the summer and winter, while the sulfate fractions of $NR-PM_1$ were calculated to be approximately 47% and 31% for the summer and winter, respectively, suggesting the possibility that particles provide non-acidic surfaces for condensation of nitric acid in the winter. The nitrate fractions of approximate 4% and 20% of $NR-PM_1$ were observed in August (summer) and November (winter), respectively, resulting that the relatively low concentration of sulfate in $NR-PM_1$ provided a non-acidic surface for nitric acid condensation and formation of particulate ammoniated nitrate is favored thermodynamically in winter. The new particle formation (NPF) event and particle growth rate were analyzed for each month in 2014 using Scanning Mobility Particle Sizer(SMPS). The Percent of NPF events was the highest in winter, but NPF event was not observed during summer due to relatively high temperature and frequent rainfall. The average particle growth rate was 3.5 nm/h and the highest particle growth rate was 5.5 nm/h in May. We observed the long-range transport of the anthropogenic sulfate from the East Asia during the intensive monitoring period of November between Qingdao and Baengnyeong Island in 2013. The relatively high concentrations of m/z 60 measured in HR-ToF-AMS was observed in May and June at Baengnyeong Island, suggesting the possibility of the influence of biomass burning from the East Asia to the Korean Peninsula.

• 한국운동역학회지
• /
• 제15권1호
• /
• pp.75-89
• /
• 2005

D. K. LEE, J. S. LEE, B. J. LEE, H. S. LEE, Y. J. KIM, S. B. PARK, J. P. JOO. Plantar foot pressure analysis during golf swing motion using plantar foot pressure measurement system. Korean Journal of Sport Biomechanics, Vol. 15, No. 1, pp. 75-89, 2005. In this study, weight carrying pattern analysis and comparison method of four foot region were suggested. We used three types of club(driver, iron7, pitching wedge). This analysis method can compare between top class golfer and beginner. And the comparison data can be used to correct the swing pose of trainee. If motion analysis system, which can measure the swing speed and instantaneous acceleration at the point of hitting a ball, is combined with this plantar foot force analysis method, new design development of golf shoes to increase comfort and ball flight distance will be available. 1. Address acting, forces concentrated in rare foot regions and lateral foot of right foot. Back swing top acting, relatively high force occurred in medial forefoot region of left foot and forefoot region of right foot. Impact acting, high force value observed in the lateral rarefoot region of left foot and medial forefoot region of right foot. Finish acting, force concentration observed on the lateral region and rarefoot region of left foot. 2. Forces were increased in address of right foot with clubs length increased. All clubs, back swing top acting, high force value observed in the lateral forefoot region of right foot. All clubs, in impact, high force value observed in the lateral rarefoot region of left foot and medial forefoot region of right foot. Finish acting, force concentration observed on the rarefoot region in driver and lateral foot region in iron on left foot. 3. Right foot forces distribution were increased in address, back swing top and left foot force distribution were increased in impact, finnish

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권9호
• /
• pp.1401-1406
• /
• 2018

Objective: The uncoupling protein 3 (UCP3) is a member of the mitochondrial anion carrier superfamily and has crucial effects on growth and feed efficiency in many species. Therefore, the objective of the present study was to examine the association of polymorphisms in the UCP3 gene with feed efficiency in meat-type chickens. Methods: Six single nucleotide polymorphisms (SNPs) of the UCP3 gene were chosen to be genotyped using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry in meat-type chicken populations with 724 birds in total. Body weight at 49 (BW49) and 70 days of age (BW70) and feed intake (FI) in the interval were collected, then body weight gain (BWG) and feed conversion ratio (FCR) were calculated individually. Results: One SNP with a low minor allele frequency (<1%) was removed by quality control and data filtering. The results showed that rs13997809 of UCP3 was significantly associated with BWG and FCR (p<0.05), and that rs13997811 had significant effects on BW70 and BWG (p<0.05). Rs13997812 of UCP3 was strongly associated with BW70, FI, and FCR (p<0.05). Furthermore, individuals with AA genotype of rs13997809 had significantly higher BWG and lower FCR (p<0.05) than those with AT genotype. The GG individuals showed strongly higher BW70 and BWG than AA birds in rs13997811 (p<0.05). Birds with the TT genotype of rs13997812 had significantly greater BW70 and lower FCR compared with the CT birds (p<0.05). In addition, the TAC haplotype based on rs13997809, rs13997811, and rs13997812 showed significant effects on BW70, FI, and FCR (p<0.05). Conclusion: Our results therefore demonstrate important roles for UCP3 polymorphisms in growth and feed efficiency that might be used in meat-type chicken breeding programs.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XII)
• /
• pp.40-44
• /
• 2003

Soil-borne infectious disease including Pythium aphanidermatum and Rhizoctonia solani causes severe damage to plants, such as cucumber. This soil-borne infectious disease was not controlled effectively by chemical pesticide. Since these diseases spread through the soil, chemical agents are usually ineffective. Instead, biological control, including antagonistic microbe can be used as a preferred control method. An efficient method was developed to select an antagonistic strain to be used as a biological control agent strain. In this new method, surface tension reduction potential of an isolate was included in the ‘decision factor’ in addition to the other factors, such as growth rate, and pathogen inhibition rate. Considering these 3 decision factors by a statistical method, an isolate from soil was selected and was identified as Bacillus sp. GB16. In the pot test, this strain showed the best performance among the isolated strains. The lowest disease incidence rate and fastest seed growth was observed when Bacillus sp. GB16 was used. Therefore this strain was considered as plant growth promoting rhizobacteria (PGPR). The action of surface tension reducing component was deduced as the enhancement of wetting, spreading, and residing of antagonistic strain in the rhizosphere. This result showed that new selection method was significantly effective in selecting the best antagonistic strain for biological control of soil-borne infectious plant pathogen. The antifungal substances against P. aphanidermatum and R. solani were partially purified from the culture filtrates of Bacillus sp. GB16. In this study, lipopeptide possessing antifungal activity was isolated from Bacillus sp. GB16 cultures by various purification procedures and was identified as a surfactin-like lipopeptide based on the Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), high performance liquid chromatography mass spectroscopy (HPLC-MS), and quadrupole time-of-flight (Q-TOF) ESI-MS/MS data. The lipopeptide, named GB16-BS, completely inhibited the growth of Pythium aphanidermatum, Rhizoctonia solani, Penicillium sp., and Botrytis cineria at concentrations of 10 and 50 mg/L, respectively. A novel method to prevent the foaming and to provide oxygen was developed. During the production of surface active agent, such as lipopeptide (surfactin), large amount of foam was produced by aeration. This resulted in the carryover of cells to the outside of the fermentor, which leads to the significant loss of cells. Instead of using cell-toxic antifoaming agents, low amount of hydrogen peroxide was added. Catalase produced by cells converted hydrogen peroxide into oxygen and water. Also addition of corn oil as an oxygen vector as well as antifoaming agent was attempted. In addition, Ca-stearate, a metal soap, was added to enhance the antifoam activity of com oil. These methods could prevent the foaming significantly and maintained high dissolved oxygen in spite of lower aeration and agitation. Using these methods, high cell density, could be achieved with increased lipopeptide productivity. In conclusion to produce an effective biological control agent for soil-borne infectious disease, following strategies were attempted i) effective screening of antagonist by including surface tension as an important decision factor ii) identification of antifungal compound produced from the isolated strain iii) novel oxygenation by $H_2O_2-catalase$ with vegetable oil for antifungal lipopeptide production.

• 항공우주정책ㆍ법학회지
• /
• 제19권1호
• /
• pp.109-139
• /
• 2004

라이트형제에 의한 비행기(飛行機)의 개발(開發)은 동세기(同世紀) 항공공학(航空工學)의 급속(急速))한 발전(發展)을 이루어 항공기를 이용하여 태평양을 횡단하고, 소리의 속도보다 빠르게 비행하게 되었으며, 수백명이 우주(宇宙) 비행(飛行)을 하였고, 10여명이 달에 다녀왔다. 그러나 이 경이로운 인간의 기술이면에는 항공기의 개발과 더불어 항공사고라는 난점이 자리하고 있다. 항공의 발달은 운송수단으로서 타 교통수단의 경쟁을 유도하였고, 항공산업의 획기적인 발달을 이룩하여 모든 여행객들과 물류수송에 선도적인 역할을 담당하고 있다. 그러나, 항공활동의 증가는 대조적으로 항공사고를 수반하게 되고, 사고시는 막대한 인명과 재산피해를 동반하게 된다. 따라서 향후 개발되는 항공기 의 초대형화와 신기술 개발 및 항공 교통량의 증가에 비례하여 항공사고건수 또한 증가할 것이며 항공사고는 인명 및 재산피해의 초대형화를 동반할 것이다. 이러한 항공사고 방지를 위해 사고조사의 전문화, 체계화 및 과학화가 필수적이다. 이를 달성하기 위한 항공사고 조사기구의 효율적인 역할을 수행하기 위하여는 사고조사위원회 위원의 상근화, 통합적사고 조사기구의 신설, 사고조사관의 제도적 신분보장, 인적요소분야 사고조사관의 확보(確保), 사고조사관의 법적 책임 한계, 사고(accident)와 중대한 준사고(serious incident)에 대한 정의와 조사범위, 그리고 민간항공기와 국가항공기가 연관된 사고 발생시 조사의 주체가 관련규정에 명시될 필요성이 개선방안(改善方案)으로 연구되었다.

• 한국멀티미디어학회논문지
• /
• 제11권9호
• /
• pp.1204-1212
• /
• 2008

가상현실은 1920년대 vehiclesimula-tion(fight simulator)을 효시로 하여 정밀한 광학적/전자기적 장치의 발달로 군사적 목적 이외의 범위로 발전하기 시작하여 realistic display 기술을 선두로 오락, 의료, 학습, 영화, 건축설계, 관광 등 다양한 분야에서 점점 일반화 되어 지고 있다. 1989년 재론 래니어에 의해 가상현실이란 용어가 고안되었고, 모든 가상 프로젝트들을 하나의 단일 항목으로 묶을 수 있게 되었지만, 그 이전에 가상현실은 연구 되어져왔고 현재에 이르게 되었으며, 불가능한 것에 대한 새로운 경험은 인간에게 가상현실의 한 부문으로써 라이드필름을 개발하게 하였다. 라이드필름(Ride Film)의 어트랙션은 기술적 요소뿐만 아니라 인간의 심리적 기반을 분석하여 그 토대로 구성되어지며, 가상현실의 궁극적인 원칙은 상호작용에 의한 몰입이다. 진정한 상호작용은 인터페이스와 입력센서 그리고 반응능력을 요구하는데, 라이드필름(Ride Film)은 전형적인 상호작용성의 요소는 아니다. 따라서 본 연구는 가상현실 기반에서 연구되어온 기술 중심적 입장 외에, 경험 중심적 정의 측면에서 사이버상의 철학자라 불리 우는 마이클 하임 교수의 7가지 기초 개념, 즉 인공현실 / 상호작용 / 몰입 / 망으로 연결된 세계 / 원격현전 /시뮬레이션 / 온몬 몰입에 근거하여 가상현실로서의 적합성을 대입 분석해 보고, 완전한 몰입을 위한 라이드필름(Ride Film)의 발전 방향과 최적화된 결론을 도출하는데 목적을 두고 있다. 이런 관점에서 라이드필름(Ride Film)의 연구는 선례연구가 부족한 라이드필름(Ride Film)의 선행연구로써의 기틀과 디지털 영상을 한 차원 발전시키는데 기여할 것이라 여긴다.

• 한국항공우주학회지
• /
• 제32권7호
• /
• pp.1-12
• /
• 2004

나비가 비행할 때 적용할 수 있는 프링-크래핑 (fling-clapping) 날개의 첫 번째 사이클 운동에 관한 흐름 가시화가 수행되었다. 본 수치적 흐름 가시화 연구에서는 프링-크랩-정지 (fling-clapping-pause), 크랩-프링-정지(clap-fling-pause)의 단계를 거치는 두 가지 형태의 날개 운동 해석을 위해 시간의존 Navier-Stokes 방정식을 이용하였다. 결과에서는 두개군의 분리와류 쌍과 그것들이 전개되는 과정과 같은 주요 흐름특성이 관찰되었다. 프링-크랩-정지 운동의 경우, 날개가 열리는 프링단계에서 시계반대방향의 강한 분리와류 쌍이 날개 사이의 열린 내부공간에 발달된다. 이어지는 크랩단계에서 분리와류 쌍은 열린 내부공간 밖으로 이동된다. 크랩-프링-정지 운동의 경우, 크랩 단계의 외부 공간에서 발달한 분리와 류 쌍은 뒤따르는 프링 단계에서 열린 내부공간내로 움직인다. 크랩-프링-정지 운동의 프 링 단계에서 발생된 열린 내부공간의 분리와류 쌍은 프링-크랩-정지 운동의 프링단계에서 발생된 열린 내부공간의 분리와류 짱보다 더 강한 와류 쌍임이 관찰되었다.

• 한국항행학회논문지
• /
• 제1권1호
• /
• pp.81-94
• /
• 1997

우리나라의 급증하는 항공수요 처리와 동북아 지역 허브 공항 역할을 하기 위한 목적으로 인천국제공항이 건설되고 있다. 그러나 동북아지역 각국에서는 대규모 공항건설 및 수용능력 확장 사업이 추진되고 있어 인천국제공항이 성공적인 허브 공항으로 자리매김하기는 쉬운 일이 아니다. 특히 일본은 우리나라에 비해 월등히 많은 항공수요량을 바탕으로 신동경국제공항(나리타 공항)에 이어 오오사카 간사이 공항을 새로운 허브 공항으로 발전시키기 위한 계획을 추진하고 있다. 따라서 인천국제공항은 일본의 공항들과 동북아지역 허브 공항 경쟁을 하여야 한다. 공항의 허브 기능은 배후지형(hinterland) 허브 기능과 모래시계형(hourglass) 허브 기능이 있다. 성공적인 배후지형 허브 공항이 되기 위해서는 근거리 국내, 국제노선에 대한 풍부한 비행편이 존재해야만 한다. 인천국제공항운영자는 동 공항과 중국, 일본, 근거리 동남아 지역 주요 도시간의 비형편이 일본의 경쟁 공항을 이용하는 것보다 유리하도록 해야 할 것이다. 모래시계형 허브 기능으로서는 지리적 위치상 원거리 동남아 지역과 미주지역을 연결시켜주는 징검다리로서의 역할이 가능하다. 여기서도 경쟁 공항과 비행편 스케쥴링 전략에서 이겨야 하는데, 공항당국과 국적 항공사의 긴밀한 협조에 의한 수요개발과 외국(미국, 동남아) 항공사들과 국적항공사(대한항공, 아시아나 항공)간의 운항 제휴 등이 효과적 스케쥴링으로 결과될 수 있을 것이다. 정부의 항공당국도 외국과의 항공협정이나 국가 항공정책 수립시 일본의 정책을 고려하고 공항 당국 및 국척항공사의 의견을 수렴하여 인천국제공항이 동북아 허브 공항으로서 성공할 수 있도록 해야할 것이다.