• Title/Summary/Keyword: In-Situ 혼합

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Development of FISH Methods for Detection of Genus Bacillus (Bacillus 속 세균을 검출하기 위한 Fluorescent In Situ Hybridization 방법의 개발)

  • 홍선희;김옥선;송홍규;이동훈;안태석
    • Korean Journal of Microbiology
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    • v.37 no.3
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    • pp.204-208
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    • 2001
  • A technique for detection of Bacillus in soil and waste water treatment system was developed. Mixed col- tured solutions of 22 Bacillus strains were applied for selection of probe and pretreatment method for FISHAmong the three probes known as useful tool for FISH method,S-G-Bacill-0597-a-A-22 was best for detec-tion of Bacillus. Ninety five percent of DAPl count was observed with FISH method with S-G-Bacill-0597-a-A-22 probe. For increasing the permeability to Bacillus cell walls, pretreatment with lysozyme was betterthan that with lysozyme and SDS/DTT (dithiothreitol). Bacillus spore was not detected with FISH. So, Bacil-lus detection in ecosystem requires FISH with pretreatment of lysozyme and spore staining.

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On the Composites of Poly(ethylene terephthalate) with a Liquid Crystalline Polyester (액정 폴리에스테르와 폴리(에틸렌 테레프탈레이트)의 복합재료 연구)

  • Choi, Jae-Kon;Bang, Moon-Soo;Han, Chul
    • Applied Chemistry for Engineering
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    • v.8 no.1
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    • pp.76-83
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    • 1997
  • Blends of thermotropic liquid crystalline polymer(TLCP) with poly(ethylene terephthalate) (PET) were prepared by the coprecipitation from a common solvent. The blends were processed through a capillary die at $287^{\circ}C$ to produce a monofilament. Morphology and mechanical, thermal properties of blends and composites were examined by differential scanning calorimetry(DSC), tensile test, optical microscopy and scanning electron microscopy. Crystallization kinetics of the blends were investigated by the isothermal DSC method. The Avrami analyses were applied to obtain the information on the crystal growth geometry and factors controlling the rate of crystallization. In the blends, liquid crystalline phase did not reveal any significant macrophase separation and thermal degradation at the processing temperature. From scanning electron micrographs of cryogenic fracture surfaces of extruded fibers, the TLCP domains were found to be more or less finely dispersed with $0.1{\mu}m$ to $0.2{\mu}m$ in size. Interfacial adhesion between the TLCP and matrix polymer was excellent. Tensile strength and modulus of TLCP/PET in-situ fiber composites were enhanced with increasing draw ratio and LCP content.

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A Study on Jointed Rock Mass Properties and Analysis Model of Numerical Simulation on Collapsed Slope (붕괴절토사면의 수치해석시 암반물성치 및 해석모델에 대한 고찰)

  • Koo, Ho-Bon;Kim, Seung-Hee;Kim, Seung-Hyun;Lee, Jung-Yeup
    • Journal of the Korean Geotechnical Society
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    • v.24 no.5
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    • pp.65-78
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    • 2008
  • In case of cut-slopes or shallow-depth tunnels, sliding along with discontinuities or rotation could play a critical role in judging stability. Although numerical analysis is widely used to check the stability of these cut-slopes and shallow-depth tunnels in early design process, common analysis programs are based on continuum model. Performing continuum model analysis regarding discontinuities is possible by reducing overall strength of jointed rock mass. It is also possible by applying ubiquitous joint model to Mohr-Coulomb failure criteria. In numerical analysis of cut-slope, main geotechnical properties such as cohesion, friction angle and elastic modulus can be evaluated by empirical equations. This study tried to compare two main systems, RMR and GSI system by applying them to in-situ hazardous cut-slopes. In addition, this study applied ubiquitous joint model to simulation model with inputs derived by RMR and GSI system to compare with displacements obtained by in-situ monitoring. To sum up, numerical analysis mixed with GSI inputs and ubiquitous joint model proved to provide most reliable results which were similar to actual displacements and their patterns.

Effect of Pine Silage Feeding on Nutrient Digestibility, Feed Conversion and Carcass Traits of Korean Native Cattle (잣나무 生枝葉사일리지 급여가 한우의 영양소 소화율, 사료요구율 및 도체성적에 미치는 영향)

  • 오영균;정찬성;이상철;김경훈;최창원;강수원;문여황
    • Journal of Animal Science and Technology
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    • v.48 no.2
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    • pp.219-226
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    • 2006
  • The wastes (the needle leaves and branches) produced from thinning the forest were fermented under an anaerobic condition (pine silage) to utilize as a forage source of ruminants. An in situ trial was conducted with two ruminally fistulated Korean native cows by 4 replicates (2 bags per cow), and in vivo digestibility of pine silage was estimated with five Korean native steers by 5 replicates in incomplete double turn-over design. In order to investigate feed efficiency and carcass traits, forty eight Korean native bulls were assigned to four treatments (0%, 25%, 50%, 75% of pine silage) with a completely randomized design in 12 pens accommodating 4 animals per pen. The amounts of concentrate and roughage allowed to experimental animals were in the range of 2.5% and 0.6% of body weight, respectively. Animals had freely accessed to mineral block and water in stanchion barn.An in situ crude protein digestibility of the roughage sources when suspended for 48 hrs in the rumen was higher for feeding the pine silage than the rice straw, whereas NDF digestibility was vice versa. No differences between the treatments were observed in in vivo digestibilities of dry matter, crude protein and NDF. Daily body weight gain was significantly (P<0.01) higher for the pine silage substitutions compared with the rice straw feeding except for the 25% silage feeding. The pine silage feeding did not affect feed efficiency.In carcass traits, marbling score tended to be higher for the 50% pine silage treatment than the other treatments while back fat thickness tended to be the thinnest for the rice straw feeding. From these results, it may be recommended to substitute pine silage, as a forage source, for rice straw by 50 percent.

The In-Situ Ozone Oxidative Remediation Potential of Diesel Fuel-contaminated Soil (디젤오염토양에 대한 지중 오존산화처리 적용 가능성)

  • 유도윤;신응배;배우근
    • Journal of Korea Soil Environment Society
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    • v.4 no.3
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    • pp.3-15
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    • 1999
  • This paper includes the basic experimental results performed for developing an innovative and technologically feasible process wherein gaseous ozone, a powerful oxidant. is injected directly into vadose zone by which in-situ chemical degradation of semi- or, non-volatile petroleum product such as diesel fuel is derived. As ozone gas injected continuously(50mL/min, 119.0$\pm$6.1mg/L) into soil packed columns artificially contaminated with diesel fuel(initial concentration 1,485mg-DRO/kg/soil), the removal rates at the inlet and outlet point of 14hrs-operated column are 87.9% and 100.0%, respectively. On the other hand, soil vapor extraction system showed less than 30% of removal rates of residual diesel both at the inlet and outlet samples under the same experimental conditions which confirms the limited treatability of SVE in diesel contaminated soil.

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Thermal behavior of modified silicon surface by $CHF_3/C_2F_6$ reactive ion etching ($CHF_3/C_2F_6$ 반응성이온 건식식각에 의해 변형된 실리콘 표면의 열적 거동에 관한 연구)

  • Park, Hyung-Ho;Kwon, Kwang-Ho;Koak, Byong-Hwa;Lee, Joong-Whan;Lee, Soo-Min;Kwon, Oh-Joon;Kim, Bo-Woo;Seong, Yeong-Gwon
    • Korean Journal of Materials Research
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    • v.2 no.1
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    • pp.35-42
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    • 1992
  • Thermal behavior of residue and damaged layer formed by reactive ion etching (RIE) in $CHF_3/C_2F_6$ were investigated using X-ray photoelectron spectroscopy(XPS) and secondary ion mass spec-trometry(SIMS) techniques. Decomposition of polymer residue film begins at $200^{\circ}C$ and above $400^{\circ}C$ carbon compound as graphite mainly forms by in-situ resistive heating. It reveals that thermal decomposition of residue can be completed by rapid thermal anneal treatment above $800^{\circ}C$ under nitrogen atmosphere and out-diffusion of carbon and fluorine of damaged layer is observed.

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A Study of Bulk Modulus of Beryl Using Water as a Pressure-Transmitting Medium (물을 압력 매개체로 이용한 녹주석의 체적탄성률 연구)

  • Hwang, Gil Chan;Kim, Hyunho;Lee, Yongjae
    • Journal of the Mineralogical Society of Korea
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    • v.30 no.3
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    • pp.83-91
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    • 2017
  • In-situ high-pressure and ex-situ high temperature-pressure experiments of natural beryl ($Be_3Al_2Si_6O_{18}$, P6/mcc) from two different localities (beryl-A and beryl-B) were studied using pure water as pressure transmitting medium. Compared to the previous study using a mixture of methanol:ethanol medium in 4 : 1 by volume, pressure- and temperature-induced chemical and structural changes under water medium are expected to be different. The derived bulk moduli are 111(7) GPa, $K{_0}^{\prime}=73(7)$; 110(9) GPa, $K{_0}^{\prime}=65(8)$ for beryl-A and beryl-B, respectively. We observe densifications in volume compression, which appear to be attributed to the phase transitions of water to ICE VI and ICE VII around 1.0 GPa and 2.5 GPa, respectively.

In-site Processing and Mechanical Properties of Ti/TiB Composites (반응생성에 의한 Ti/TiB 복합재료의 제조와 기계적 성질)

  • Jeong, Hui-Won;Lee, Yong-Tae
    • Korean Journal of Materials Research
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    • v.9 no.3
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    • pp.307-314
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    • 1999
  • The effect of manufacturing variables, such as reactant powder$(TiB_2, B_4C)$, sintering temperature, and sintering time has been investigated on the microstructure and the mechanical properties of in-situ processed Ti/TiB composites. The mechanical properties were evaluated by measuring the compressive yield strength. The compressive yield strength of the in-situ processed composites was higher than that of the Ti-6AI-4V. The compressive yield strength of the composite made with TiE, reactant powder was higher than that of $B_4C$, mixed at the same volume fraction of reinforcement. It is because bonding nature between the matrix and the $TiB_2$, reactant powder was more strong than that of the other materials. It was proven by the examining the crack propagation path.

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Characteristics of the Heteroepitaxial $Si_{1-x}Ge_{x}$ Films Grown by RTCVD Method (RTCVD 법으로 성장한 $Si_{1-x}Ge_{x}$ 에피막의 특성)

  • Chung, W.J.;Kwon, Y.K.;Bae, Y.H.;Kim, K.I.;Kang, B.K.;Sohn, B.K.
    • Journal of Sensor Science and Technology
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    • v.5 no.2
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    • pp.61-67
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    • 1996
  • The growth and characterization of heteroepitaxial $Si_{1-x}Ge_{x}$ films grown by the RTCVD (Rapid Thermal Chemical Vapor Deposition) method were described. For the growth of $Si_{1-x}Ge_{x}$ heteroepitaxial layers, $SiH_{4}$ / $GeH_{4}$ / $H_{2}$ gas mixtures were used. The growth conditions were varied to investigate their effects on the Si / Ge composition ratios, the interface abruptness and crystalline properties. The experimental data shows that the misfit threading dislocation in $Si_{1-x}Ge_{x}$ / Si heteroepitaxial film of about $400\;{\AA}$ thickness was not observed at the growth temperature of as low as $650^{\circ}C$, and the composition ratios of Si / Ge changed linearly with $SiH_{4}$ / $GeH_{4}$ gas mixing ratios in our experimental ranges. In the in-situ boron doping experiments, the doping abruptness would be controlled within several hundreds ${\AA}$/decade.

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Experimental Study for the Development of the Mixing Ratio as a Compaction Pile (다짐말뚝 재료로서 쇄석과 저회의 적정 혼합비 도출을 위한 실험적 연구)

  • Leem, Hansoo;Kim, Sunkon;Lee, Jooho;Chun, Byungsik
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.9
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    • pp.5-16
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    • 2012
  • In the case of using the soil materials created by cutting in-situ ground directly without adjusting particle size, it is recommendable to seek the compaction property or material constant required for filling design or density control through indoor test, and many studies on this subject have been carried out during that time. The researches conducted during that time, however, were focused on the mixed materials with different diameters that exist in a natural condition. There has been no study conducted using coal fly ash that is by-product of the thermal power plant that is actively considered as the building materials. Therefore, this study was aimed at implementing compaction test and examining the basic engineering property in order to explore the influence of crushing the particles through compacting the admixture of crushed stone and coal fly ash produced from thermal power plant on its engineering property, and then the impact of the admixture volume of each material on compaction property and material property by conducting the One-Dimensional Compression Test. As result of compaction test, the optimum moisture ratio of coal fly ash was shown to be approx. 23%. As result of compaction test in accordance with the mixed ratio of coal fly ash and crushed stone under the same compaction energy and moisture ratio, dry unit weight tended to drop when the mixed ratio of coal fly ash exceeded 30%, while it reached approx. $1.81gf/cm^3$ when the mixed ratio was 30%. As result of One-Dimensional Compression Test in accordance with the mixed ratio of crushed stone and coal fly ash, the change in void ratio by particle crushing was at the highest level in the case of coal fly ash 100%, while the lowest level in the case of crushed stone 100%. In the case of mixed materials of crushed stone and coal fly ash, compression index was at the lowest level in case of coal fly ash 30%, and therefore this ratio of mixed material was judged to be the most stable from an engineering aspect.