• Title/Summary/Keyword: In situ experiment

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Effect of Several Adsorbents on The Gastrointestinal Absorption of Paraquat

  • Machijima, Hiraku;Nakamura, Hiroyuki;Hatanaka, Tomomi;Sugibayashi, Kenji;Morimoto, Yasunori
    • Archives of Pharmacal Research
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    • v.17 no.5
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    • pp.287-293
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    • 1994
  • The effect of several adsorbents on paraquat poisohing was investigated (1) by measuring the saturatd amount of the poison adhered on the adsorbents in vitro and (2) by assaying the blood level of paraquat in the rat in in situ intestinal absorption experiments. Activated charcoal powder, natural aluminum silifonate) were used as adorbents. The steady-state blood level of paraquat in its absorption experiment with the cationic exchange resins was markedly lower than those without the resins or with other adsorbents. A good relationship was achieved between the calculated AUC or adsorptioin rate (in situ) and the saturated adsorption amount (in vitro). The rank order of the effect was sodium polystyene sulfonate > calcium polystyene sulfonate>natural aluminum silicate>activated charcoal powder. The effect of sodium polystyrene sulfonate after intestinal washing with physiological saline ws also measured, and a synergistic effect (marked decrease in blood paraquat level0 was found as compared with the intestinal washing alone. The simultaneous use of G.I. weshing and powerful adsorbent was scientifically proven to be most benefical.

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Kimchi cabbage (Brassica rapa L.) by-products treated with calcium oxide and alkaline hydrogen peroxide as feed ingredient for Holstein steers

  • Son, A-Rang;Kim, Seon-Ho;Valencia, Raniel A.;Jeong, Chang-Dae;Islam, Mahfuzul;Yang, Chul-Ju;Lee, Sang-Suk
    • Journal of Animal Science and Technology
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    • v.63 no.4
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    • pp.841-853
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    • 2021
  • This study aimed to investigate the effects of Kimchi cabbage by-products either treated or untreated with calcium oxide (CaO) and alkaline hydrogen peroxide (AHP) as substitutional ingredient of total mixed ration (TMR) on in vitro fermentation, in situ disappearance and growth performance of Holstein steers. Cannulated Holstein (600 ± 47 kg) was used for both the in vitro and in situ experiments. The treatments used were TMR only (CON), TMR + 30% Kimchi cabbage by-products fresh matter (FM) basis (TC), TMR + 30% Kimchi cabbage by-products FM basis + 5% CaO FM basis (TCC), and TMR + 30% Kimchi cabbage by-products FM basis + 5% CaO FM basis + 3.22% AHP FM basis (TCCA). For in vivo experiment, thirty-four Holstein steers (273 ± 45 kg) were subjected to a 150-day feeding trial, divided into two groups: CON and TC. In the in vitro experiment, pH of TCCA was greatest (p < 0.05) among other treatments at all incubation times. Ammonia nitrogen and volatile fatty acid concentrations were not significantly different for each treatment. However, butyrate was greater (p < 0.05) in TCC and CON than in both TC and TCCA. During in situ experiment, the dry matter (DM) disappearance was greatest (p < 0.05) in TCCA among other treatments. Also, disappearance of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were observed greatest (p > 0.05) in TCCA treatment. In the in vivo experiment, average daily gain (ADG) did not differ between CON and TC. In blood profile analysis, alanine aminotransferase, aspartate aminotransferase, glucose, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and total protein concentration were not significantly different between treatments. But, creatinine concentration was greater (p < 0.05) in TC than in CON. Overall results suggest that Kimchi cabbage by-products either treated or untreated with CaO and AHP can be used as substitutional ingredient in TMR for Holstein steers.

Low-Soil Disturbance In-Situ Test Method Development and Its Application : Screw Plate Loading Test (지반의 교란을 최소화 한 원위치시험법 개발 및 적용 : 스크류재하시험)

  • Lee, Yong-Su;Hwang, Woong-Ki;Choi, Yong-Kyu;Kim, Tae-Hyung
    • Proceedings of the Korean Geotechical Society Conference
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    • 2009.03a
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    • pp.977-986
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    • 2009
  • Sampling disturbance can introduce considerable errors in the laboratory estimation of geotechnical properties of soils, and the results obtained from sophisticated sampling and careful laboratory testing are not matching with field behavior. Therefore, it is advantage to adopt in-situ testing techniques for the estimation of geotechnical parameters. Therefore, Screw plate loading test, one of new field test technologies, has been investigated in this study. This test can be utilized to find out important properties of soils such as load-displacement, elastic modulus, and shear strength. The screw plate loading test modified from the plate loading test is an experiment underneath ground by inserting a spiral type of auger screw. The structure and characteristics of the screw plate loading test device was examined in detail. In addition, The new screw plate loading test device was manufactured to refer the previous studies. The reliability of developing screw plate loading test was examined through the analysis of the laboratory test.

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Development of a Time-selective Self-triggering Water Sampler and Its Application to In-situ Calibration of a Turbidity Sensor

  • Jin, Jae-Youll;Hwang, Keun-Choon;Park, Jin-Soon;Yum, Ki-Dai;Oh, Jae-Kyung
    • Journal of the korean society of oceanography
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    • v.34 no.4
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    • pp.200-206
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    • 1999
  • Seawater sampling is the primary task for the study of the marine environmental parameters that require shipboard or laboratory experiments for their analyses, and is also required for the calibration of some instruments for in situ measurement. A new automatic bottle (AUTTLE) is developed for seawater sampling at any desired time and water depth by self-triggering. Both any type of single or assembled mooring for 15 days and manual actuation by using a remote messenger as existing instantaneous single point water samplers are possible. Its sampling capacity and the resolution of time setting are 2 liters and 1 second, respectively. The result of a field experiment with an optical backscattering sensor (OBS) and a total of 14 AUTTLES for the in situ calibration of the OBS shows that the AUTTLE must improve our understanding on the behavior of the sand/mud mixtures in the environments with high waves and strong tides. The AUTTLE will serve as a valuable instrument in the various fields of oceanography, especially where synchronized seawater sampling at several sites is required and/or the information in storm period is important.

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Study on Correlation Between Feed Protein Fractions and In situ Protein Degradation Rate (사료 단백질의 Fraction과 In situ 단백질 분해율의 상관관계에 관한 연구)

  • Lee, S.Y.;Chung, Y.S.;Song, J.Y.;Park, S.H.;Sung, H.G.;Kim, H.J.;Ko, J.Y.;Ha, Jong-Kyu
    • Journal of Animal Science and Technology
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    • v.49 no.3
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    • pp.351-358
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    • 2007
  • This experiment was conducted to determine correlation between in vitro protein fractions and in situ protein degradation rate with major dairy protein sources(soybean meal, corn gluten meal, cotton seed meal, kapok seed meal and perilla meal). Five protein fractions were obtained according to the Cornell Net Carbohydate and Protein System(CNCPS), and in situ protein degradation rates were determined by technique using nylon bags incubated for 0, 4, 8, 12 and 24hrs in the rumen of three Holstein steers. Fraction A was highest in kapok seed meal(14.6%) and lowest in corn gluten meal(0.6%) (P<0.05). The highest B1, B2 and B3 fractions were contained in soybean meal(8.27%), cotton seed meal(74%), and perilla meal(40%), respectively. Corn gluten meal was very high in fraction C. In situ protein degradation rate of soybean meal was 98%, highest among five protein sources, and corn gluten meal had the lowest rate at 28%. Correlation analysis showed that easily soluble fractions of both methods, in situ protein degradation rate and digestible protein fractions, and in situ protein degradation rate minus “a” and fraction B2+B3 were highly correlated. These results indicate that in vitro protein fractionation can be used in the estimation of in situ protein degradation.

Effects of Different Microbial Culture Supplements on In vitro and In situ Ruminal Fermentation Characteristics of Italian ryegrass Silage (미생물제 처리에 의한 이탈리안 라이그라스 사일리지의 In vitro 및 In situ 반추위 발효특성에 미치는 영향)

  • Lim, D.H.;Ki, K.S.;Choi, S.H.;Kim, T.I.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.4
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    • pp.309-317
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    • 2016
  • The study was conducted to evaluate the effects of microbial culture supplements on ruminal fermentation and fermentative quality of Italian ryegrass silage (IRGS) both in vitro and in situ. Three species of microbes (Lactobacillus casei (LC), Bacillus subtilis (BS), and Saccharomyces cerevisiae (SC)) were used in this study. They were applied to IRGS at 30 days after silage manufacture. Various items were measured using in vitro and in situ incubation technique after each microbial supplement was inoculated into IRGS at $0.5{\times}10^4CFU/g$. In the first experiment, in vitro ruminal fermentation characteristics of IRGS were evaluated at 0, 12, 24, 48, and 72 hours after microbes were inoculated into IRGS. In the second experiment, in situ fermentation characteristics were investigated at 0, 1, 3, and 5 days after the inoculation of each microbial supplement. In vitro ruminal $NH_3-N$ content was significantly (p<0.05) increased in LC-, BS-, and SC-IRGS at 12 hrs post incubation compared to that in control IRGS. In vitro ruminal total VFA concentration and dry matter digestibility (DMD) of IRGS were not significantly difference among LC-, BS-, and SC-IRGS, although they were numerically increased in LC-IRGS than those of the other IRGS. In addition, this study evaluated the fermentation characteristics and in situ DMD of IRGS with the lapse of incubation time up to 5 days. Throughout the incubation times from 1 day to 5 days, the pH value was significantly (p<0.05) lower in BS-, LC-, and SC-IRGS than that in control IRGS. Lactate was significantly (p<0.05) higher, and significantly (p<0.05) butyrate was lower in LC-IRGS than that in other treatments at 0 day. It was higher (p<0.05) in control IRGS than that of BS-, LC-, and SC-IRGS at 1-5 days. In situ DMD tended to increase in BS-, LC-, and SC-IRGS compared to that in control IRGS. Especially, DMD was higher in SC-IRGS than that in other treatments at 0 day. It tended to be higher in LC-IRGS at all incubation time. Taken together, these results suggest that it might be useful to select a microorganism by considering the feeding time of IRGS to ruminants because organic acids and DMD of IRGS were affected by the incubation time of each microorganism with IRG silage, especially for L. casei decreased the content of acetate and butyrate in IRGS.

The Analysis of Inflowing Sediments Behavior around the Sandbar in Nakdong River Estuary (낙동강 하구역 사주 주변에서의 퇴적물질의 유압거동 해석)

  • KIM KYUNG-HOI;LEE IN-CHEOL
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2004.11a
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    • pp.133-136
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    • 2004
  • In-situ collection experiments were performed to analyze inflowing sedilnents behavior as a basic study of topographical change in Nakdong River Estuary. Sediment rate and bed load flux ranged from 0.0004 to $0.472\;g/cm^2/day\;and\;0.0005\~1.7579\;g/cm^2/day$, respectively. The settling velocity of suspended particulate matter was estimated in tire range of $0.339\~1.010\;cm/sec$, The grain size analysis shows that surface sediments in backside of sandbar and bed load in front of dike have a similar grain size distribution. It is considered that the source of surface sediments in backside of sandbar were flowed in from Nakdong River. In order to verify the characteristics of inflowing sediments behavior, detailed surveys around Nakdong River Estuary need to be carried out, continuously.

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A Study on the Applicability of Resistivity Cone for Contaminant Investigation in Farm Land (농경지 오염탐사를 위한 비저항콘의 적용성에 관한 연구)

  • Yu, Chan;Yoon, Chun-Gyeong
    • Journal of Korean Society of Rural Planning
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    • v.5 no.1 s.9
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    • pp.45-55
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    • 1999
  • The applicability of resistivity cone for contaminant investigation in farm land was examined in laboratory and in-situ. To verify the resistivity variations in sand with contaminants, laboratory experiment used soil resistivity test box was performed. Heavy metal and others ions were appeared steep variations in low concentration. Pilot test results were the same as laboratory test results. In the in-situ test used resistivity cone, resistivities of waste landfill layer were low than the common soil layers and resistivities had been difference with concentration of contaminant components. Therefore contaminant investigation in farm land using resistivity cone was expected very effective.

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Overturning Resistance of Plain Concrete Piers in OSPG Railroad Bridges

  • Rhee, In-Kyu;Park, Joo-Nam;Choi, Eun-Soo
    • International Journal of Railway
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    • v.3 no.1
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    • pp.1-6
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    • 2010
  • The steel plate-girder bridges with concrete gravity piers have possibilities of overturning by lateral inertial force which can be reproduced by sudden earthquake attack. This paper explores an overturning mechanism of existing concrete gravity pier onto the sandy soil in the event of lateral push-over load by in-situ experimental observation. The in-situ push-over experiment for pier with earth anchors between spread footing and rock beds exhibits a reasonable enhancement of ductility against overturning. In unanchored system, a flexural crack at cold joint of concrete pier is not developed because of the over-turning of the pier. This leads a global instability (rotation) of pier-footing system with relatively low stresses in pier itself. While a lateral load is persistently increased in anchored system, the successive flexural cracking failure at cold joint is observed even after the local shear failure of soil due to redistribution of stress equilibrium between soil and pier structure as long as a tensile action of anchor cable is active.

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Laboratory experiment on the assessment of the ground strength with corestone (실내실험을 통한 핵석지반의 강도정수 산정연구)

  • 이수곤;김동은;황의성
    • Proceedings of the Korean Geotechical Society Conference
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    • 2003.03a
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    • pp.95-102
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    • 2003
  • Corestone rock mass has complex characters because it is made up of stronger and stiffer corestone in a weaker and softer matrix. Physical model corestone rock mass made up of stiffer corestone in weaker matrix were tested in uniaxial compression and numercal modelling analysis The result of the uniaxial compression tests showed that increasing the corestone proportion generally increased the modulus of deformation. And the strength decreased in the lower corestone proportion, but it increased in the higher proportion(45%, 65% corestone by volume). The strength and the modulus of deformation were not affected by different size coretone on the same proportion. The result of the numerical modelling analysis showed similar trend compared with the result of the result of the uniaxial compression test. But though the result of th uniaxial compression test is similar to the result of the numerical modelling analysis, it's unreasonalble to apply the results of this paper to in situ corestone rock mass. So mere laboratory tests including triaxial test and the other numerical program analyses are necessary to apply the results to in situ corestone mass

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