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http://dx.doi.org/10.5187/JAST.2007.49.3.351

Study on Correlation Between Feed Protein Fractions and In situ Protein Degradation Rate  

Lee, S.Y. (School of Agricultural Biotechnology, Seoul National University)
Chung, Y.S. (School of Agricultural Biotechnology, Seoul National University)
Song, J.Y. (School of Agricultural Biotechnology, Seoul National University)
Park, S.H. (School of Agricultural Biotechnology, Seoul National University)
Sung, H.G. (School of Agricultural Biotechnology, Seoul National University)
Kim, H.J. (School of Agricultural Biotechnology, Seoul National University)
Ko, J.Y. (Livestock and Feed Research Institute, National Agricultural Cooperative Federation)
Ha, Jong-Kyu (School of Agricultural Biotechnology, Seoul National University)
Publication Information
Journal of Animal Science and Technology / v.49, no.3, 2007 , pp. 351-358 More about this Journal
Abstract
This experiment was conducted to determine correlation between in vitro protein fractions and in situ protein degradation rate with major dairy protein sources(soybean meal, corn gluten meal, cotton seed meal, kapok seed meal and perilla meal). Five protein fractions were obtained according to the Cornell Net Carbohydate and Protein System(CNCPS), and in situ protein degradation rates were determined by technique using nylon bags incubated for 0, 4, 8, 12 and 24hrs in the rumen of three Holstein steers. Fraction A was highest in kapok seed meal(14.6%) and lowest in corn gluten meal(0.6%) (P<0.05). The highest B1, B2 and B3 fractions were contained in soybean meal(8.27%), cotton seed meal(74%), and perilla meal(40%), respectively. Corn gluten meal was very high in fraction C. In situ protein degradation rate of soybean meal was 98%, highest among five protein sources, and corn gluten meal had the lowest rate at 28%. Correlation analysis showed that easily soluble fractions of both methods, in situ protein degradation rate and digestible protein fractions, and in situ protein degradation rate minus “a” and fraction B2+B3 were highly correlated. These results indicate that in vitro protein fractionation can be used in the estimation of in situ protein degradation.
Keywords
Protein fractions; In situ protein degradation; Correlation;
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