• Applied Microscopy
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• v.38 no.4
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• pp.383-386
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• 2008

In performing in situ heating transmission electron microscopy (TEM) for materials characterizations, arising concerns such as specimen drifts and unintentional Cu contamination are discussed. In particular, we analysed the thermal and mechanical characteristics of in situ heating holders to estimate thermal drift phenomena. From the experimental results, we suggest an empirical model to describe the thermal drift behavior so that we can design an effective plan for in situ heating experiment. Practical approaches to minimize several hindrances arisen from the experiment are proposed. We believe that our experimental recommendations will be useful for a microscopist fascinated with the powerful potential of in situ heating TEM.

• Geomechanics and Engineering
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• v.16 no.4
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• pp.363-373
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• 2018

The evaluation of Thermo-Hydro-Mechanical (THM) coupling behavior is important for the development of underground space for various purposes. For a high-level radioactive waste repository excavated in a deep underground rock mass, the accurate prediction of the complex THM behavior is essential for the long-term safety and stability assessment. In order to develop reliable THM analysis techniques effectively, an international cooperation project, Development of Coupled models and their Validation against Experiments (DECOVALEX), was carried out. In DECOVALEX-2015 Task B2, the in situ THM experiment that was conducted at Horonobe Underground Research Laboratory(URL) by Japan Atomic Energy Agency (JAEA), was modeled by the research teams from the participating countries. In this study, a THM coupling technique that combined TOUGH2 and FLAC3D was developed and applied to the THM analysis for the in situ experiment, in which rock, buffer, backfill, sand, and heater were installed. With the assistance of an artificial neural network, the boundary conditions for the experiment could be adequately implemented in the modeling. The thermal, hydraulic, and mechanical results from the modeling were compared with the measurements from the in situ THM experiment. The predicted buffer temperature from the THM modelling was about $10^{\circ}C$ higher than measurement near by the overpack. At the other locations far from the overpack, modelling predicted slightly lower temperature than measurement. Even though the magnitude of pressure from the modeling was different from the measurements, the general trends of the variation with time were found to be similar.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2002.10a
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• pp.357-360
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• 2002

A in-situ experiment was performed to evaluate the pullout resistance capacity of chains which is used as a reinforcement of reinforced earth wall. It was also considered that chain was combined with a bar or L-type steel angle by the transverse reinforcement member in the experiment. As a result of experiment, it is expected that chain can be safely used as reinforcements of reinforced earth wall, although it is concerned that a theoretical estimation of the pullout resistance capability of chain is too conservative.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2005.04a
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• pp.58.1-58.1
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• 2005

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.20 no.4
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• pp.455-468
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• 2022

Colloid Formation and Migration (CFM) project is being carried out within the Grimsel Test Site (GTS) Phase Ⅵ. Since 2008, the Korea Atomic Energy Research Institute (KAERI) has joined CFM to investigate the behavior of colloid-facilitated radionuclide transport in a generic Underground Research Laboratory (URL). The CFM project includes a long-term in-situ test (LIT) and an in-rock bentonite erosion test (i-BET) to assess the in-situ colloid-facilitated radionuclide transport through the bentonite erosion in the natural flow field. In the LIT experiment, radionuclide-containing compacted bentonite was equipped with a triple-packer system and then positioned at the borehole in the shear zone. It was observed that colloid transport was limited owing to the low swelling pressure and low hydraulic conductivity. Therefore, a postmortem analysis is being conducted to estimate the partial migration and diffusion of radionuclides. The i-BET experiment, that focuses more on bentonite erosion, was newly designed to assess colloid formation in another flow field. The i-BET experiment started with the placement of compacted bentonite rings in the double-packer system, and the hydraulic parameters and bentonite erosion have been monitored since December 2018.

• Applied Microscopy
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• v.45 no.2
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• pp.95-100
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• 2015

The in-situ heating transmission electron microscopy experiment allows us to observe the time- and temperature-dependent dynamic processes in nanoscale materials by examining the same specimen. The temperature, which is a major experimental parameter, must be measured accurately during in-situ heating experiments. Therefore, calibrating the thermocouple readout of the heating holder prior to the experiment is essential. The calibration can be performed using reference materials whose phase-transformation (melting, oxidation, reduction, etc.) temperatures are well-established. In this study, the calibration experiment was performed with four reference materials, i.e., pure Sn, Al-95 wt%Zn eutectic alloy, NiO/carbon nanotube composite, and pure Al, and the calibration curve and formula were obtained. The thermocouple readout of the holder used in this study provided a reliable temperature value with a relative error of <4%.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.7
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• pp.929-939
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• 2011

The effects of micronization on in situ and in vitro nutrient disappearances of wheat, barley and corn were investigated in a series of experiments. In Experiment 1, chemical composition and in situ dry matter disappearance (DMD) of six varieties of wheat were determined. In addition, an in vitro study was completed using ground micronized and unmicronized wheat (var. Kansas). In Experiment 2, three varieties of wheat (Kansas, Sceptre and Laura) and in Experiment 3, three cereal grains (wheat, barley and corn) were either micronized for 1 min to attain internal kernel temperatures of 90-100$^{\circ}C$ or not (controls), and DM, protein and starch disappearances were estimated. In Experiment 2, an in vitro study was also completed using ground micronized and unmicronized wheat (var. Kansas). Wheat samples varied with respect to crude protein (10.0-21.2%), starch (61.6-73.9%), NDF (8.5-11.8%), volume weight (753-842 g/L) and kernel hardness (0.0-32.0). Rate (p = 0.003) and extent (p = 0.001) of in situ DMD differed among wheat varieties. Correlations between in situ kinetics, and chemical and physical properties of wheat varieties showed that protein content was negatively correlated with the rate of disappearance ($r^2$ = -0.77). Micronization of all grains markedly reduced (p = 0.001) the rate and extent of DM, and protein disappearances as compared to control samples. Micronization increased (p<0.05) the digestion of starch in wheat. However, release of ammonia into the incubation medium was markedly reduced (p<0.05), suggesting that micronization increased the resistance of protein to microbial digestion. Disappearances of DM, protein and starch differed (p = 0.001) among cereal grains with wheat>barley>corn. Micronization reduced the rate of DM disappearance (p = 0.011) and slowly degradable protein fractions (p = 0.03), however, increased (p = 0.004) slowly degradable starch fractions of all three cereals. Examination of in situ samples by scanning electron microscopy confirmed that microbial colonization focused on starch granules in micronized grains, and that the protein matrix exhibited resistance to microbial colonization. These results suggest that micronization may be used to increase the ruminal escape value of protein in cereal grains, but may lead to increased starch digestion if grains are finely ground.

• Applied Microscopy
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• v.43 no.2
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• pp.88-97
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• 2013

Technical investigation to figure out the problems arising during in-situ heating electron backscatter diffraction (EBSD) analysis inside scanning electron microscopy (SEM) was carried out. EBSD patterns were successfully acquired up to $830^{\circ}C$ without degradation of EBSD pattern quality in steels. Several technical problems such as image drift and surface microstructure pinning were taking place during in-situ experiments. Image drift problem was successfully prevented in constant current supplying mode. It was revealed that the surface pinning problem was resulted from the $TiO_2$ oxide particle formation during heating inside SEM chamber. Surface pinning phenomenon was fairly reduced by additional platinum and carbon multi-layer coating before in-situ heating experiment, furthermore was perfectly prevented by improvement of vacuum level of SEM chamber via leakage control. Plane view in-situ observation provides better understanding on the overall feature of recrystallization phenomena and cross sectional in-situ observation provides clearer understanding on the recrystallization mechanism.

• Biomolecules & Therapeutics
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• v.22 no.2
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• pp.161-165
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• 2014

The main purpose of this study was to develop a novel, in situ gel system for sustained delivery of ranitidine hydrochloride. Ranitidine in situ gels at 0.2%, 0.5%, and 1.0% gellan gum concentration (w/v) were prepared, respectively, and characterized in terms of preparation, viscosity and in vitro release. The viscosity of the gellan gum formulations in solution increased with increasing concentrations of gellan gum. In vitro study showed that the release of ranitidine from these gels was characterized by an initial phase of high release (burst effect) and translated to the second phase of moderate release. Single photon emission computing tomography technique was used to evaluate the stomach residence time of gel containing $^{99m}Tc$ tracer. The animal experiment suggested in situ gel had feasibility of forming gels in stomach and sustained the ranitidine release from the gels over the period of at least 8 h. In conclusion, the in situ gel system is a promising approach for the oral delivery of ranitidine for the therapeutic effects improvement.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1994.11a
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• pp.54-58
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• 1994

H$_2$ bake and GeH$_4$ clean are used as a in-situ pre-clean method in low temperature Si based epitaxial growth technology using rapid thermal processing chemical vapor deposition (RTPCVD). In this paper, the H$_2$ bake and GeH$_4$ clean processes are optimized for low surface defect density using Taguchi method. In H$_2$ bake process, the epitaxial growth temperature affects dominantly on the surface defect density, and the next affecting factors are H$_2$ bake temperature and rinse time in de-ionised water. In GeH$_4$ clean process, GeH$_4$ clean temperature affects most strongly on the surface defect density, and the minor factor is GeH$_4$flow rate. The optimum process conditions predicted fly Taguchi method agree well with tile experimental data in both in-situ clean processes.