The objective of this study was to manufacture the wood based roughage using lumber from thinning of oak and pitch pine (Pinus rigida). And the study also aimed to investigate a feed value evaluation of wood based roughages. To investigate the optimization condition of steam-digestion treatment for roughage, the wood chips of oak and pitch pine were steam-digestion treated at $160^{\circ}C$ under pressure 6 atm depending on treatment times (60 min, 90 min and 120 min) followed by the content of essential oils analyzed. The essential oil content of steam-digestion treated roughages for 90 min and 120 min were under 0.1 mL/kg. The evaluation of feed value was carried out from steam-digestion treated roughages for 90 min through feed chemical composition analysis, NRC (National research Council) modeling, ruminal degradability analysis and relative economic value analysis. The feed chemical compositions including DM (dry mater), CP (crude protein), EE (ether extract), NDF (neutral detergent fiber), ADF (acid detergent fiber), ADL (acid detergent lignin), NFC (nonfiber carbohydrate) in oak roughage were 95.4, 1.36, 3.11, 90.05, 83.85, 17.33, 6.50%, respectively, and in pitch pine roughage were 94.37, 1.33, 5.48, 87.89, 86.88, 30.56, 6.32%, respectively. Both roughages showed low level of protein and very high level of NDF. The TDN (total digestible nutrient) levels using NRC (2001) model in oak and pitch pine roughages were 40.55, 31.22%, respectively. The ruminal in situ dry matter degradability was higher in oak roughage (23.84%) than in pitch pine roughage (10.02%). The economic values of oak and pitch pine rough-ages were 235, and 210 \, respectively.
Kim Yong Seok;Woo Chong Kyu;Lee Yong Sung;Koh Jai Kyung;Chun Ha Chung;Lee Myung Za
Radiation Oncology Journal
/
v.14
no.4
/
pp.265-279
/
1996
Damage produced by radiation elicits a complex response in mammalian cells, including growth rate changes and the induction of a variety of genes associated with growth control and apoptosis. At doses of 10,000 cGy or greater, the exposed individual was killed in a matter of minutes to a couple of days, with symptoms consistent with pathology of the central nervous system(CNS) including degenerative changes. The nature of the damage in irradiated cells underlies the unique hazards of ionizing radiation. Radiation injury to CNS is a rare event in clinical medicine, but it is catastrophic for the patient in whom it occurs. The incidence of cerebral necrosis has been reported as high as 16% for doses greater than 6,000 cGy. In this study, the effect of radiation on brain tissue was studied in vivo. Jun and p53 genes in the rat brain were induced by whole body irradiation of rat with 600Co in doses between 1 Gy and 100 Gy and analyzed for expression of jun and p53 genes at the postirradiation time up to 6 hours. Northern analyses were done using 1.8 Kb & 0.8 Kb-pGEM-2-JUN/Eco RI/Pst I fragments, 2.0 Kb-php53B/Bam HI fragment and ,1.1 Kb-pBluescript SK--ACTIN/Eco RI fragment as the digoxigenin or [${\alpha}^{32}P$] dCTPlabeled probes for Jun, p53 and ${\beta}$-actin genes, respectively. Jun gene seemed to be expressed near the threshold levels in 1 hour after irradiation of $^{60}$Co in dose less than 1 Gy and was expressed in maximum at 1 hour after irradiation of $^{60}$Co in dose of 30 Gy. Jun was expressed increasingly with time until 5 or 6 hours after irradiation of $^{60}$Co in doses of 1 Gy and 10 Gy. After irradiation of $^{60}$Co in dose between 20 Gr and 100 Gy, the expression of Jun was however increased to peak in 2 hours and decreased thereafter. p53 gene in this study also seemed to be expressed near the threshold levels in 1 hour after irradiation of $^{60}$Co in dose less than 1 Gy and was expressed in maximum at 6 hours after irradiation of $^{60}$Co in dose of 1 Gy, p53 was expressed increasingly with time until 5 or 6 hours after irradiation of $^{60}$Co in dose between 1 Gy and 40 Gy. After irradiation of $^{60}$Co in doses of 50 Gy and 100 Gy, the expression of p53 was however increased to peak in 2 hours and decreased thereafter. The expression of Jun and p53 genes was not correlative in the brain tissue from rats. It seemed to be very important for the establishment of the optimum conditions for the animal studies relevant to the responses of genes inducible on DNA damage to ionizing radiation in mammalian cells. But there are many limitations to the animal studies such as the ununiform patterns of gene expression from the tissue because of its complex compositions. It is necessary to overcome the limitations for development of in situ Northern analysis.
Park, Kyung-Ae;Park, Ji-Eun;Choi, Byoung-Ju;Byun, Do-Seong;Lee, Eun-Il
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
/
v.18
no.4
/
pp.234-265
/
2013
Oceanic current maps in the secondary school science and earth science textbooks have played an important role in piquing students's inquisitiveness and interests in the ocean. Such maps can provide students with important opportunities to learn about oceanic currents relevant to abrupt climate change and global energy balance issues. Nevertheless, serious and diverse errors in these secondary school oceanic current maps have been discovered upon comparison with up-to-date scientific knowledge concerning oceanic currents. This study presents the fundamental methods and strategies for constructing such maps error-free, through the unification of the diverse current maps currently in the textbooks. In order to do so, we analyzed the maps found in 27 different textbooks and compared them with other up-to-date maps found in scientific journals, and developed a mapping technique for extracting digitalized quantitative information on warm and cold currents in the East Sea. We devised analysis items for the current visualization in relation to the branching features of the Tsushima Warm Current (TWC) in the Korea Strait. These analysis items include: its nearshore and offshore branches, the northern limit and distance from the coast of the East Korea Warm Current, outflow features of the TWC near the Tsugaru and Soya Straits and their returning currents, and flow patterns of the Liman Cold Current and the North Korea Cold Current. The first draft of the current map was constructed based upon the scientific knowledge and input of oceanographers based on oceanic in-situ measurements, and was corrected with the help of a questionnaire survey to the members of an oceanographic society. In addition, diverse comments have been collected from a special session of the 2013 spring meeting of the Korean Oceanographic Society to assist in the construction of an accurate current map of the East Sea which has been corrected repeatedly through in-depth discussions with oceanographers. Finally, we have obtained constructive comments and evaluations of the interim version of the current map from several well-known ocean current experts and incorporated their input to complete the map's final version. To avoid errors in the production of oceanic current maps in future textbooks, we provide the geolocation information (latitude and longitude) of the currents by digitalizing the map. This study is expected to be the first step towards the completion of an oceanographic current map suitable for secondary school textbooks, and to encourage oceanographers to take more interest in oceanic education.
Kim, Tae-Sung;Park, Kyung-Ae;Choi, Won-Moon;Hong, Sungwook;Choi, Byoung-Cheol;Shin, Inchul;Kim, Kyung-Ryul
Korean Journal of Remote Sensing
/
v.28
no.5
/
pp.477-487
/
2012
Sea surface winds in the sea off the east coast of Korea were derived from L-band ALOS (Advanced Land Observing Satellite) PALSAR (Phased Array type L-band Synthetic Aperture Radar) data and their characteristics of errors were analyzed. We could retrieve high-resolution wind vectors off the east coast of Korea including the coastal region, which has been substantially unavailable from satellite scatterometers. Retrieved SAR-wind speeds showed a good agreement with in-situ buoy measurement by showing relatively small an root-mean-square (RMS) error of 0.67 m/s. Comparisons of the wind vectors from SAR and scatterometer presented RMS errors of 2.16 m/s and $19.24^{\circ}$, 3.62 m/s and $28.02^{\circ}$ for L-band GMF (Geophysical Model Function) algorithm 2009 and 2007, respectively, which tended to be somewhat higher than the expected limit of satellite scatterometer winds errors. L-band SAR-derived wind field exhibited the characteristic dependence on wind direction and incidence angle. The previous version (L-band GMF 2007) revealed large errors at small incidence angles of less than $21^{\circ}$. By contrast, the L-band GMF 2009, which improved the effect of incidence angle on the model function by considering a quadratic function instead of a linear relationship, greatly enhanced the quality of wind speed from 6.80 m/s to 1.14 m/s at small incident angles. This study addressed that the causes of wind retrieval errors should be intensively studied for diverse applications of L-band SAR-derived winds, especially in terms of the effects of wind direction and incidence angle, and other potential error sources.
Kim, Moon Su;Kim, Tae Seung;Kim, Hyun Koo;Kim, Dong Su;Jeong, Do Hwan;Ju, Byoung Kyu;Hong, Jung Ki;Kim, Hye Jin;Park, Sun Hwa;Jeong, Chan Ho;Cho, Byong Wook;Han, Jin Seok
Journal of Soil and Groundwater Environment
/
v.18
no.4
/
pp.19-31
/
2013
To figure out the decay characteristics of naturally occurring radionuclides, eight sampled groundwaters from a monitoring borehole having high levels of uranium and radon concentrations in a two mica granitic area have analyzed by liquid scintillation counters (LSC) for over 1 year. In December 2011, three groundwater samples (DJ1, DJ2, DJ3) were obtained from each aquifer system located at -20 m, -40 m, -60 m of the monitoring borehole below the ground surface, respectively. Five samples (DJ4, DJ5, DJ6, DJ7, DJ8) were additionally gained from each aquifer positioned -20 m, -40 m, -60 m, -100 m, -105 m of the borehole in February 2012, respectively. Temporal variation characteristics of uranium and radon concentrations have showed over maximum 2.1 times and 1.4 times fluctuations of the values in the same sampling intervals over time, respectively. The intervals of -40 m and -105 m in the borehole have the highest values of uranium and radon concentrations, respectively. This may imply that the concentrations of naturally occurring radionuclides such as uranium and radon in groundwater have been changed over time and indicate that the qualities of groundwaters from the aquifers developed at each interval in the borehole are different each other. This discrepancy, moreover, could be caused by behaviour differences between uranium which is in ionic status having a half life of 4.6 billion years and is transported along with the flowing groundwater, and radon which is in gaseous status having a 3.82 day's half life in the aquifer systems. Physicochemical characteristics of groundwaters from the aquifer systems could be identified by the results of the on-situ measuring items such as pH and Eh, and the major ionic contents. The CPM values of eight groundwater samples analysed by LSC over one year have shown not to follow the theoretical decay curve of the radon. The CPM values of the samples have ranged from 2 to 7.5 after it had passed two months when the theoretical CPM values of the radon started zero since the initial analysis. Alpha and beta particle spectrums have shown the peaks of radium-226, however they have not revealed any peaks of radon and it's daughter products such as polonium-218 and 214, bismuth-214 for the late stage of the analysis. This implies that the groundwater from the borehole may contain radium-226 having a half life of 1,600 years which decays continuously.
Recently in Korea, application of the soil nailing is gradually extended to the sites of excavations and slopes having various ground conditions and field characteristics. Design of the soil nailing is generally carried out in two steps, The First step is to examine the minimum safety factor against a sliding of the reinforced nailed-soil mass based on the limit equilibrium approach, and the second step is to check the maximum displacement expected to occur at facing using the numerical analysis technique. However, design parameters related to the soil nailing system are so various that a reliable design method considering interrelationships between these design parameters is continuously necessary. Additionally, taking into account the anisotropic characteristics of in-situ grounds, disturbances in collecting the soil samples and errors in measurements, a systematic analysis of the field measurement data as well as a rational technique of the optimum design is required to improve with respect to economical efficiency. As a part of these purposes, in the present study, a procedure for the optimum design of a soil nailing excavation wall system is proposed. Focusing on a minimization of the expenses in construction, the optimum design procedure is formulated based on the genetic algorithm. Neural network theory is further adopted in predicting the maximum horizontal displacement at a shotcrete facing. Using the proposed procedure, various effects of relevant design parameters are also analyzed. Finally, an optimized design section is compared with the existing design section at the excavation site being constructed, in order to verify a validity of the proposed procedure.
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
/
v.13
no.1
/
pp.42-55
/
2008
To evaluate the accuracy of currents measured by HF radar in the coastal sea off Keum River estuary, we compared the facing radial vectors of two HF radars, and HF radar-derived currents with in-situ measurement currents. Principal component analysis was used to extract regression line and RMS deviation in the comparison. When two facing radar's radial vectors at the mid-point of baseline are compared, RMS deviation is 4.4 cm/s in winter and 5.4 cm/s in summer. When GDOP(Geometric Dilution of Precision) effect is corrected from the RMS deviations that is analyzed from the comparison between HF radar-derived and current-metermeasured currents, the error of velocity combined by HF radar-derived current is less than 5.1 cm/s in the stations having moderate GDOP values. These two results obtained from different method suggest that the lower limit of HF radar-derived current's accuracy is 5.4 cm/s in our study area. As mentioned in previous researches, RMS deviations become large in the stations located near the islands and increase as a function of mean distance from the radar site due to decrease of signal-to-noise level and the intersect angle of radial vectors. We found that an uncertain error bound of HF radar-derived current can be produced from the separation process of RMS deviations using GDOP value if GDOP value for each component is very close and RMS deviations obtained from current component comparison are also close. When the current measured in the stations having moderate GDOP values is separated into tidal and subtidal current, characteristics of tidal current ellipses analyzed from HF radar-derived current show a good agreement with those from current-meter-measured current, and time variation of subtidal current showed a response reflecting physical process driven by wind and density field.
Kang M. Y.;Han M. S.;Lee S. C.;Kim J. H.;Sohn S. H.
Reproductive and Developmental Biology
/
v.29
no.1
/
pp.1-7
/
2005
Telomeres consisting of (TTAGGG)n tandem repeat DNA sequences and associated proteins are essential for chromosome stability and related with cell senescence, apoptosis and cancer. The telomerase is a ribonucleoprotein which act as a template for the synthesis of telomeric DNA. This study was carried out to identify the distribution of telomeres on mouse chromosomes and also to analyze the amount of telomeres and telomerase activity of mouse embryos at early embryonic stages. Germ cells and early embryos from 1 cell to blastocyst stage were analyzed. The amount of telomeres was analyzed by quantitative fluorescence in situ hybridization technique(Q-FISH) using a human telomeric DNA probe, and telomerase activity was measured by telomeric repeat amplification protocol assay(TRAP). In results, the telomeres on mouse chromosomes were distributed at the ends of all autosomes and sex chromosomes. Although the quantity of telomeres varied among chromosomes, most of chromosomes had higher amount in q-arm telomeres than in p-arm telomeres. The results of Q-FISH indicated that the relative amount of telomeres of mouse embryos in each embryonic stage was approximately the same except the higher amount in blastocysts. Using TRAP assay on mouse embryos, telomerase activity was detected in all preimplantation stages from mature oocytes to blastocysts. Especially the telomerase activity was significantly increased at the morula and blastocyst stage. In conclusion, there may be a close association between the amount of telomeres and telomerase activity in early embryonic stages, and analysis of telomere quantity and telomerase activity on early development will be helpful for the investigation of embryogenesis and embryonic cell differentiation in mice.
Journal of the Korean Association of Geographic Information Studies
/
v.17
no.2
/
pp.156-172
/
2014
This research measures and compares on-site net radiation energy, air temperature, wind speed, and surface temperature considering various spatial characteristics with a focus on land use types in urban areas in Changwon, Southern Gyeongsangnam-do, to analyze the accuracy of an ENVI-met model, which is an analysis program of microclimate. The on-site measurement was performed for three days in a mobile measurement: two days during the daytime and one day during the nighttime. The analysis using the ENVI-met model was also performed in the same time zone as the on-site measurement. The results indicated that the ENVI-met model showed higher net radiation than the on-site measurement by approximately $300Wm^{-2}$ during the daytime whereas the latter showed higher net radiation energy by approximately $200Wm^{-2}$ during the nighttime. The temperature was found to be much higher by approximately $2-6^{\circ}C$ in the on-site measurement during both the daytime and nighttime. The on-site measurement also showed higher surface temperature than the ENVI-met by approximately $7-13^{\circ}C$. In terms of the wind speed, there was a significant difference between the results of the ENVI-met model and on-site measurement. As for the correlation between the results of the ENVI-met model and on-site measurement, the temperature showed significantly high correlation whereas the correlations for the net radiation energy, surface temperature, and wind speed were very low. These results appear to be affected by excessive or under estimation of solar and terrestrial radiation and climatic conditions of the surrounding areas and characteristics of land cover. Hence, these factors should be considered when applying these findings in urban and environment planning for improving the microclimate in urban areas.
Lee, Ji Eun;Moon, Kwang Bin;Hwang, Jong Hee;Kwon, Eun Kyung;Kim, Sun Hee;Kim, Jong Won;Jin, Dong Kyu
Clinical and Experimental Pediatrics
/
v.45
no.9
/
pp.1126-1133
/
2002
Purpose : Prader-Willi syndrome(PWS) is a complex disorder affecting multisystems with characteristic clinical features. Its genetic basis is an expression defect in the paternally derived chromosome 15q11-q13. We analyzed the clinical features and genetic basis of PWS patients for early detection and treatment. Methods : We retrospectively studied 24 patients with PWS in Department of Pediatrics, Samsung Medical Center, from September 1997 to September 2001. We performed cytogenetic and molecular genetic techniques using high resolution GTG banding techniques, fluorescent in situ hybridization and methylation-specific PCR for CpG island of SNRPN gene region. Results : The average birth weight of PWS patients was $2.67{\pm}0.47kg$ and median age at diagnosis was 1.3 years. The average height and weight of PWS patients under one year at diagnostic time were located in a 3-10 percentile relatively, and a rapid weight gain was seen between two and six years. Feeding problems in infancy and neonatal hypotonia were the two most consistently positive major criteria in over 95% of the patients. In 18 of the 24 cases(75%), deletion of chromosome 15q11-q13 was demonstrated and one case among 18 had an unbalanced 14;15 translocation. In four cases without any cytogenetic abnormality, it may be considered as maternal uniparental disomy and the rest showed another findings. Conclusion : We suggest diagnostic testing for PWS in all infants/neonates with unexplained feeding problems and hypotonia. It is necessary for clinically suspicious patients to undergo an early genetic test. As the genetic basis of PWS was heterogenous and complex, further study is required.
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