• Title/Summary/Keyword: Improved Experiments

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Effects of Feeding Aspergillus oryzae Ferments on Performance, Intestinal Microflora, Blood Serum Components and Environmental Factors in Broiler (Aspergillus oryzae 배양물의 급여가 육계의 생산성, 장내미생물, 혈청성분 및 계사환경 요인에 미치는 영향)

  • 김상호;박수영;유동조;이상진;류경선;이동규
    • Korean Journal of Poultry Science
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    • v.30 no.3
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    • pp.151-159
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    • 2003
  • Two experiments were carried out to evaluate the effects of feeding Aspergillus oryzae(AO) ferment on performance, intestinal microflora, serum components, ammonia generation and litter dampness in broiler chicks. In experiment I, three hundred sixty, one day old broiler chicks, Abor Acres, were fed 0 and 0.1% of Aspergiilus oryzae short conidia ferment(AOS) and 0.1% of Aspergillus oryzae long conidia ferment(AOL) for five weeks. In experiment II, three hundred sixty, one day old broiler chicks, Abor Acres were fed 0, 0.1 and 0.2% of Aspergillus oryzae long conidia ferment(AOL) for five weeks. In experiment I, growth rates were not statistically different among dietary treatments. AOS and AOL showed increased tendency in weight gain and feed intake compared to those of control, whereas feed conversion was not different. Litter dampness of AOS and AOL was also tended to decrease compared to that of control, but was not significantly different. Fecal ammonia gas generation was decreased in feeding AOS and AOL, and maintained 1/2 to 3/4 compared to the control. In serum metabolites, AOS and AOL increased glucose and calcium, and decreased total protein, blood urea nitrogen and total cholesterol. In experiment II, body weight of chicks fed 0.1 and 0.2% AOL were heavier than the control(P<0.05). Feed intake of chicks fed 0.1 and 0.2% AOL also were higher than the none, but feed conversion ratio was not different among treatments. Ileal and cecal microflora showed increased tendency in lactic acid bacteria compared to those of the control. Salmonella and E. coli were decreased in ileum of chicks fed 0.1 and 0.2% AOL. In conclusion, feeding AO ferment increased growth performance and improved intestinal microflora of broiler chicks and environments of broiler house.

Effect of Feeding Plum and Red Ginseng Marc on Vital Reaction in Broiler Stress (매실과 홍삼 부산물이 스트레스에 대한 육계 생체 반응에 미치는 영향)

  • Bong, Mi-Hee;Ji, Sang-Yoon;Park, Jun-Cheol;Moon, Hong-Kil;Lee, Sang-Cheul;Lee, Jun-Heon;Hong, Joon-Ki
    • Korean Journal of Poultry Science
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    • v.38 no.3
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    • pp.213-223
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    • 2011
  • This study was conducted to test the efficacy of plum (Prunus mume) and red ginseng (Panax ginseng C.A Meyer) marc as stress inhibitors under heat stress and lipopolysaccharide (LPS) challenge in broilers by investigating their effects on blood biochemical parameters, immunoglobulin concentration and splenic cytokine mRNA expressions. A total of one hundred ninety-two 1-d-old male broiler chicks (Ross 308) were divided into 2 stress conditions (heat and LPS) experiments. Each experiment was divided into 4 treatment groups with 8 replicates of 3 birds in each group. NC (negative control, no immune substances), PC (positive control, 25 ppm ${\beta}$-glucan), PM (1% plum marc) and RGM (3% red ginseng marc) treatments were administered with respective substance through water supplementation. During heat stress, The Ca/Mg ratio in PM and RGM was significantly decreased in comparison with that of NC (P<0.05). The immunoglobulin M was significantly lower in PM than in NC (P<0.05). Expression patterns of splenic cytokine mRNAs (IL-1, IL-2 and IL-6) were similar over the treatment. Expression rates of IL-1 and IL-2 in PM were significantly decreased in comparison with NC. Also, expression rates of IL-1, IL-2 and IL-6 were significantly lower in RGM than in NC (P<0.05). In conclusion, the dietary supplementation of plum and red ginseng marc improved coping ability to heat stress by preventing Ca/Mg ratio increment and by inhibiting inflammatory response in broiler chicks. However, it is necessary to determine optimal dietary level of red ginseng marc for improving growth performances in broiler chickens. These results suggest the possibility that plum and red ginseng marc could be used as the stress inhibitor under heat stress and inflammatory response in broiler chicks.

Target Word Selection Disambiguation using Untagged Text Data in English-Korean Machine Translation (영한 기계 번역에서 미가공 텍스트 데이터를 이용한 대역어 선택 중의성 해소)

  • Kim Yu-Seop;Chang Jeong-Ho
    • The KIPS Transactions:PartB
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    • v.11B no.6
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    • pp.749-758
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    • 2004
  • In this paper, we propose a new method utilizing only raw corpus without additional human effort for disambiguation of target word selection in English-Korean machine translation. We use two data-driven techniques; one is the Latent Semantic Analysis(LSA) and the other the Probabilistic Latent Semantic Analysis(PLSA). These two techniques can represent complex semantic structures in given contexts like text passages. We construct linguistic semantic knowledge by using the two techniques and use the knowledge for target word selection in English-Korean machine translation. For target word selection, we utilize a grammatical relationship stored in a dictionary. We use k- nearest neighbor learning algorithm for the resolution of data sparseness Problem in target word selection and estimate the distance between instances based on these models. In experiments, we use TREC data of AP news for construction of latent semantic space and Wail Street Journal corpus for evaluation of target word selection. Through the Latent Semantic Analysis methods, the accuracy of target word selection has improved over 10% and PLSA has showed better accuracy than LSA method. finally we have showed the relatedness between the accuracy and two important factors ; one is dimensionality of latent space and k value of k-NT learning by using correlation calculation.

Studies on Processing and Keeping Quality of Retort Pouched Foods (5) Preparation and Keeping Quality of Retort Pouched Seasoned Ark Shell (레토르트파우치 식품의 가공 및 품질안정성에 관한 연구 (5) 레토르트파우치 조미피조개제품의 제조 및 품질안정성)

  • LEE Eung-Ho;OH Kwang-Soo;AHN Chang-Bum;LEE Tae-Hun;CHUNG Young-Hoon;SHIN Keun-Jin;KIM Woo-Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.19 no.2
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    • pp.109-117
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    • 1986
  • For the purpose of obtaining basic data which can be applied to processing of retort pouched shellfishes, retort pouched seasoned ark shell, Anadara broughtonii, was prepared. The frozen ark shell was thawed and seasoned with a mixed seasoning powder prepared with $10.0\%$ of sorbitol, $2.0\%$ of table salt and $0.5\%$ of monosodium glutamate at $5^{\circ}C$ for 10 hours, and then dried at $45^{\circ}C$ for 4 hours. The dried seasoned ark shell was coated with $1.0\%$ sodium alginate solution, dried with cola air blast for 2 hours and then vacuum-packed in the laminated plastic film bag (polyester/casted polypropylene= $12{\mu}m/70{\mu}m,\;15{\times}16cm$), and finally sterilized up to Fo=6.0 in hot water circulating retort at $121^{\circ}C$ for 10 minutes. The major fatty acids of raw ark shell and retort pouched seasoned ark shell products were 16:0, 20:5, 22:6, 18:0 and 18:3, and predominant free amino acids of those were lysine, arginine, glycine, alanine, glutamic acid and leucine. In nucleotides and its related compounds of raw ark shell and retort pouched seasoned ark shell products, the most abundant one was AMP, and total extract-N of those was chiefly consisted of free amino acids, betaine and nucleotide and its related compounds. During the processing procedure such as drying and sterilization, unsaturated fatty acids slightly decreased while saturated fatty acids increased, and total extract-N content decreased about a half. From the results of chemical and microbial experiments during storage, it was concluded that the products could be preserved in a good condition for 100 days at room temperature, and their duality could be improved by the coating treatment of sodium alginate solution.

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A Study on the Method of Minimizing the Bit-Rate Overhead of H.264 Video when Encrypting the Region of Interest (관심영역 암호화 시 발생하는 H.264 영상의 비트레이트 오버헤드 최소화 방법 연구)

  • Son, Dongyeol;Kim, Jimin;Ji, Cheongmin;Kim, Kangseok;Kim, Kihyung;Hong, Manpyo
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.28 no.2
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    • pp.311-326
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    • 2018
  • This paper has experimented using News sample video with QCIF ($176{\times}144$) resolution in JM v10.2 code of H.264/AVC-MPEG. The region of interest (ROI) to be encrypted occurred the drift by unnecessarily referring to each frame continuously in accordance with the characteristics of the motion prediction and compensation of the H.264 standard. In order to mitigate the drift, the latest related research method of re-inserting encrypted I-picture into a certain period leads to an increase in the amount of additional computation that becomes the factor increasing the bit-rate overhead of the entire video. Therefore, the reference search range of the block and the frame in the ROI to be encrypted is restricted in the motion prediction and compensation for each frame, and the reference search range in the non-ROI not to be encrypted is not restricted to maintain the normal encoding efficiency. In this way, after encoding the video with restricted reference search range, this article proposes a method of RC4 bit-stream encryption for the ROI such as the face to be able to identify in order to protect personal information in the video. Also, it is compared and analyzed the experimental results after implementing the unencrypted original video, the latest related research method, and the proposed method in the condition of the same environment. In contrast to the latest related research method, the bit-rate overhead of the proposed method is 2.35% higher than that of the original video and 14.93% lower than that of the latest related method, while mitigating temporal drift through the proposed method. These improved results have verified by experiments of this study.

A Study on the Development and Physical Properties of Low Yellowing Epoxy for Ceramic Preservation (도자기 복원용 저황변 Epoxy 수지의 개발 및 물성에 대한 연구)

  • Kim, Woo Hyun;Cheong, Da Som;Bae, Jin Soo;Jee, Joo Yeon;Wi, Koang Chul
    • Journal of Conservation Science
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    • v.30 no.2
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    • pp.181-187
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    • 2014
  • AThe studies on ceramic preservation have been conducted widely in various institutions such as national/ public museums and research labs as well as the excavation institutions and university museums. Although there are some differences in preservation methodologies and materials used across the institutions, the variation is minimal. Specifically, epoxy resin is mostly used for ceramic restoration for its high cohesiveness, low contraction and high strength although there are some variations in for Ceramic Preservation. The synthetic resin type used according to the type of damage in the ceramic. However, the yellowing or the change of color across the time after the restoration is the weakness of epoxy resin. In this study, we aim to develop a material which minimizes this yellowing of epoxy resin while enhancing its cohesiveness and strength as well as other physical properties. We made the new material to have similar properties with those used widely for the ceramic restoration, such as EPO-TEK301$^{(R)}$, L30$^{(R)}$, XTR-311$^{(R)}$ through comparative experiments. The cohesiveness of the newly developed resin was improved to 2.51(MPa), which is similar level of XTR-311$^{(R)}$ of the 2.30(MPa) but about 2x higher than the other resins EPO-TEK301$^{(R)}$, L30$^{(R)}$ (1.21 and 1.81 (MPa), respectively). Especially, the experiment on yellowing shows that the existing resins show the range of color change at 10~25(${\Delta}E^*ab$), but the new low yellowing epoxy resin has the color change value at 8.3 (${\Delta}E^*ab$), the value lowering the yellowing effect to 1 to 3 times of the existing epoxy resin, thereby solving the issue of generating sense of differences due to change of color or yellowing.

Enhanced Anaerobic Degradation of Food Waste by Employing Rumen Microorganisms (Rumen 미생물을 이용한 주방폐기물 혐기성소화의 효율증진 방안)

  • Shin, Hang-Sik;Song, Young-Chae;Son, Sung-Sub;Bae, Byung-Uk
    • Journal of the Korea Organic Resources Recycling Association
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    • v.1 no.1
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    • pp.103-113
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    • 1993
  • Every year, over $3.37{\times}10^7$ ton of municipal solid waste is generated in Korea, of which about 28% is organic food waste from restaurant, dining halls and households etc. Methane conversion of the food waste by anaerobic digestion could be a viable approach for energy recovery as well as safe disposal of the waste. However, as food waste is composed of highmolecular complex polymers such as cellulose, lignin and protein, anaerobic digestion of food waste has not been efficient in terms of volumetric loading rate, solid retention time and extent of anaerobic degradation. In this research, the improved anaerobic degradation of food waste was attemped by applying rumen microorganisms to anaerobic digestion. Acidification efficiency of food waste by rumen microorganisms was compared with that of conventional acidogenesis. And optimum acidification conditions by rumen microorganisms were also determined. For the experiments, anaerobic batch reactors of 600 mL was fed with the processed (dried and milled) food waste obtained from a restaurant. Ultimate volatile fatty acid (VFA) yield produced by rumen microorganisms was about 8.4 meq VFA/g volatile solid (VS) that is 95% of the theoretical value. This yield was not much different from that of conventional acidogenesis, but hydrolysis rate was about twice faster. Cumulative VFA concentration increased from 66 meq/L to 480 meq/L, when the initial TS was increased from 1% to 15%. But VFA yield at 15% TS was half of that at 1% TS. This inhibition on the acidification might be caused by the rapid drop of pH and higher concentration of nonionized VFA. Optimal pH and temperature range for the acidification were about 6.0~7.5 and $35{\sim}45^{\circ}C$, respectively.

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Improvement of Straw Loading Method on Survival of Mouse IVF/IVC Blastocysts Cryopreserved by Vitrification (체외수정과 체외배양에서 생산된 생쥐 배반포기배의 초자화 동결에 있어서 Straw Loading 방법의 개선)

  • 김선의;엄상준;김은영;윤산현;박세필;임진호
    • Korean Journal of Animal Reproduction
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    • v.20 no.1
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    • pp.35-42
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    • 1996
  • This study was carried out to investigate the effect of straw loading method and thawing protocol on the in vitro development of in vitro produced mouse blastocysts cryopreserved by vitrification. Three loading types of straw I, Il and III on loading and sealing method were made for vitrification. The ability of the solution on straw loading methods to remain vitreous during warming was tested by exposed in air for 1 to 10 s sec. and then plunged the vitrified straws into water bath at 25°C. Embryos to be vitrified were equilibrated to the 20% EG for 5min. and exposed in EFS 40 for 1min. The plug ends of Straw I and Straw II were sealed with straw powder and Straw III was treated straw powder, followed by heat sealing and then plunged into LN$_2$. The results obtained in these experiments were summarized as follows; 1) Straw I embryo column mostly changed from transparent to opaque upon thawing without exposure in air for 3-6 sec. Straw II embryo column was I improved partially but was not remained completely vitreous during warming. However, when Straw lll loading method was used, the embryo column was remained vitreous completely. 2) High survival rates and development rates of each groups (middle blastocysts and hatching blastocysts) of vitrified embryos were obtained by using Straw III loading method than Straw I method (P<0.05). And the range of s standard error was low in Straw lll method. 3) When the embryos vitrified-frozen were placed in air for 3, 5 and l0sec. and then warmed rapidly in water bath at 25$^{\circ}C$, the survival rates after 24h of culture were 72.7-87.1% and the development rates to hatching stage after 48h of culture were 34.0-48.4%. There were no significantly differences according to exposure time in air during warming. In conclusion, the present results showed that highly survival and low standard error of vitrified-frozen mouse bIastocysts were obtained by using straw lll loading, double sealing and appropriate 2 step warming method.

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Effect of Dietary Supplementation of Yeast Culture on the Performance, Nutrient Digestibility and Physico-Chemical characteristics of the Pork in Growing-Finishing Pigs (효모배양물의 수준별 급여가 육성·비육돈의 생산성, 영양소 소화율, 돈육의 이화학적 특성 및 지방산 조성에 미치는 영향)

  • Park, J. H.;Lim, O. C.;Na, C. S.;Ryu, K. S.
    • Journal of Animal Science and Technology
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    • v.45 no.2
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    • pp.219-228
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    • 2003
  • Two experiments were conducted to investigate the influence of dietary supplementation of yeast culture on the nutrient digestibility, performance and meat quality in growing-finishing pigs. Corn-soy basal diets contained 20, 18, and 16% CP and 3,265kcal/kg ME at different growth stages. One hundred and twenty pigs(8wk-old) were allocated into four supplementation levels of yest culture (0, 0.1, 0.2, 0.4%) in Expt 1. Weight gain, feed intake and feed efficiency were periodically recorded for 112 days. A metabolic feeding trial was conducted to measure the nutrient digestibility. Physical and chemical characteristics of the Longissimus Dorsi muscle(LM) from the pigs were measured at the end of experiment (Expt 2). In Expt 1, weight gain and feed efficiency were not different among the dietary treatment groups during the overall period. The digestibilities of protein and fiber were greater in 0.1% and 0.2% yeast culture-supplemented groups than in control (P<0.05). However, fat and ash digestibilities were not improved by the dietary treatment. In Expt 2, the LM protein content in 0.1% yeast culture-supplemented group, but not in 0.2% or 0.4%-supplemented group, was greater than that in the control group (P<0.05). The LM pH, purge loss, cooking loss and color were not affected by the dietary treatment. The LM shearing force was less in 0.2% yeast culture-supplemented group than in the control group (P<0.05). Other LM physicochemical properties did not differ between the dietary treatment groups. Total saturated and unsaturated fatty acids percentages and total cholesterol content of the LM did not differ across the dietary treatment groups.

Extraction & Purification of ${\beta}$-carotene from Recombinant Escherichia coli (재조합 대장균으로부터 고순도 베타-카로틴의 추출 및 정제)

  • Jo, Ji-Song;Nguyen, Do Quynh Anh;Yun, Jun-Ki;Kim, Yu-Na;Kim, You-Geun;Kim, Sung-Bae;Seo, Yang-Gon;Lee, Byung-Hak;Kang, Moon-Kook;Kim, Chang-Joon
    • Microbiology and Biotechnology Letters
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    • v.37 no.3
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    • pp.231-237
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    • 2009
  • This paper aimed to develop a solvent extraction and purification process to recover high-purified ${\beta}$-carotene from recombinant Escherichia coli. Cells harvested from the culture broth were treated through numerous steps: dehydration, solvent extraction, crystal formation and separation. To optimize the extracting condition, experiments were carried out to investigate the effect of cell disruption, temperature, organic solvents, solvent-biomass ratio on the yield of ${\beta}$-carotene extracted from cells. The result indicated that no significant differences of extraction yield were observed from cells with or without step of cell disruption. Among different extracting solvents, the highest extraction yield of ${\beta}$-carotene, 30.3 mg-${\beta}$-carotene/g-dry cells, was obtained with isobutyl acetate at solvent-biomass ratio 25 mL/g-dry cells at $50^{\circ}C$. Notably, in case of acetone, the extraction yield was quite low when using acetone itself, but increased almost up to the highest value when combining this solvent and olive oil. The purity of ${\beta}$-carotene crystals obtained from crystallization and separation was 89%. The purity degree was further improved up to 98.5% by treating crude crystals with additional ethanol washing.