• Fisheries and Aquatic Sciences
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• 제19권5호
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• pp.26.1-26.8
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• 2016

Background: The nematode species belonging to genus Anisakis occur at their third larval stage in numerous marine teleost fish species worldwide and known to cause accidental human infection through the ingestion of raw or undercooked fish or squids. They may also draw the attention of consumers because of the visual impact of both alive and dead worms. Therefore, the information on their geographical distribution and clear species identification is important for epidemiological survey and further prevention of human infection. Results: For identification of anisakid nematodes species isolated from largehead hairtail (Trichiurus japonicus), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of internal transcribed spacers of ribosomal DNA were conducted. Mitochondrial cytochrome c oxidase subunit 2 gene was also sequenced, and phylogenetic analysis was conducted. From the largehead hairtail (n = 9), 1259 nematodes were isolated in total. Most of the nematodes were found encapsulated throughout the viscera (56.2 %, 708/1259) or moving freely in the body cavity (41.5 %, 523/1259), and only 0.3 % (4/1259) was found in the muscles. By PCR-RFLP, three different nematode species were identified. Anisakis pegreffii was the most dominantly found (98.7 %, 1243/1259) from the largehead hairtail, occupying 98.7 % (699/708) of the nematodes in the mesenteries and 98.1 % (513/523) in the body cavity. Hybrid genotype (Anisakis simplex ${\times}$ A. pegreffii) occupied 0.5 %, and Hysterothylacium sp. occupied 0.2 % of the nematodes isolated in this study. Conclusions: The largehead hairtail may not significantly contribute accidental human infection of anisakid nematode third stage larvae because most of the nematodes were found from the viscera or body cavity, which are not consumed raw. But, a high prevalence of anisakid nematode larvae in the largehead hairtail is still in concern because they may raise food safety problems to consumers. Immediate evisceration or freezing of fish after catch will be necessary before consumption.

• 패션비즈니스
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• 제16권3호
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• pp.13-31
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• 2012

The purpose of the study was to analyze the work clothes' clothing comfort and wearer mobility of painting workers with the consideration of the work environment features in the machine and shipbuilding industries in South Korea. A questionnaire survey was conducted for the study, which consisted of questions on the clothing comfort and wearer mobility aspects of painting work clothes by clothes types and body parts. The work clothes' clothing comfort and wearer mobility levels were scaled in 5 points i.e. 1(: very tight/very uncomfortable) to 5(: very slack/very comfortable). The painting work environmental hazardous features were considered as high impact levels of workplace temperature, oxygen deficiency, organic solvent, toxic gas factors while metal fragment factor only impacts 'low' in the painting processes with the findings throughout this study. Since the painting work consisted of surface washing and the spray and touch-up painting processes, which was carried out in an outdoor work place, the painting work clothes should meet high performance of waterproofing from the painting material and air permeability specially in summer as well as thermal performance in winter. The subjects painting workers' assessment of the existing work clothes' clothing oppression was in the levels between 3 (i.e. moderate) and 4 (i.e. comfortable) in a range of 1 to 5 points. The existing painting work clothes' wearer mobility was evaluated 'very uncomfortable' in all work clothes parts, especially, armhole length, biacromial breadth, sleeve length of the jumper; and body rise, waist, hip, thigh and knee circumferences of the pants.

• Asian Pacific Journal of Cancer Prevention
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• 제17권2호
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• pp.647-653
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• 2016

Background: Breast cancer is the most common type of cancer affecting Malaysian women. Recent statistics revealed that the cumulative probability of breast cancer and related deaths in Malaysia is higher than in most of the countries of Southeast Asia. Single nucleotide polymorphisms (SNPs) in CYP2E1 (rs6413432 and rs3813867), STK15 (rs2273535 and rs1047972) and XRCC1 (rs1799782 and rs25487) have been associated with breast cancer risk in a meta-analysis but any link in Southeast Asia, including Malaysia, remained to be determined. Hence, we investigated the relationship between these SNPs and breast cancer risk among Malaysian women in the present case-control study. Materials and Methods: Genomic DNA was isolated from peripheral blood of 71 breast cancer patients and 260 healthy controls and subjected to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Results: Our study showed that the c1/c2 genotype or subjects with at least one c2 allele in CYP2E1 rs3813867 SNP had significantly increased almost 1.8-fold higher breast cancer risk in Malaysian women overall. In addition, the variant Phe allele in STK15 rs2273535 SNP appeared to protect against breast cancer in Malaysian Chinese. No significance association was found between XRCC1 SNPs and breast cancer risk in the population. Conclusions: This study provides additional knowledge on CYP2E1, STK15 and XRCC1 SNP impact of risk of breast cancer, particularly in the Malaysian population. From our findings, we also recommend Malaysian women to perform breast cancer screening before 50 years of age.

• Archives of Plastic Surgery
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• 제39권1호
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• pp.31-35
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• 2012

Background : Orbital roof fractures are frequently associated with a high energy impact to the craniofacial region, and displaced orbital roof fractures can cause ophthalmic and neurologic complications and occasionally require open surgical intervention. The purpose of this article was to investigate the clinical features and treatment outcomes of orbital root fractures combined with neurologic injuries after early reconstruction. Methods : Between January 2006 and December 2008, 45 patients with orbital roof fractures were admitted; among them, 37 patients were treated conservatively and 8 patients underwent early surgical intervention for orbital roof fractures. The type of injuries that caused the fractures, patient characteristics, associated fractures, ocular and neurological injuries, patient management, and treatment outcomes were investigated. Results : The patients underwent frontal craniotomy and free bone fragment removal, their orbital roofs were reconstructed with titanium micromesh, and associated fractures were repaired. The mean follow up period was 11 months. There were no postoperative neurologic sequelae. Postoperative computed tomography scans showed anatomically reconstructed orbital roofs. Two of the five patients with traumatic optic neuropathy achieved full visual acuity recovery, one patient showed decreased visual acuity, and the other two patients completely lost their vision due to traumatic optic neuropathy. Preoperative ophthalmic symptoms, such as proptosis, diplopia, upper eyelid ptosis, and enophthalmos were corrected. Conclusions : Early recognition and treatment of orbital roof fractures can reduce intracranial and ocular complications. A coronal flap with frontal craniotomy and orbital roof reconstruction using titanium mesh provides a versatile method and provides good functional and cosmetic results.

• 대한기계학회논문집A
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• 제39권6호
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• pp.575-581
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• 2015

드릴비트는 암반에 직접적으로 타격력과 회전력을 전달함으로써 연속적으로 암반을 파쇄, 분쇄하는 천공기의 핵심 구성요소이다. 일정한 속도로 회전하는 동시에 회전축 방향으로 타격하며 암반을 파쇄하기 때문에 암반 파쇄효율을 높이기 위해서는 드릴비트의 배열 최적설계가 필요하다. 그러므로 본 연구에서는 버튼배열 최적화를 수행하기 위한 선행연구로써 버튼배열의 성능을 정량적으로 평가할 수 있는 방법에 대한 연구를 수행하였다. 버튼의 중복타격 면적, 비 타격 면적, 천공편향 모멘트와 같은 평가지표를 활용하여 버튼배열의 파쇄 효율성을 평가하였으며, 더불어 제안된 평가방법을 활용하여 기존 드릴비트 버튼배열의 파쇄 효율성을 검토함으로써 제안한 배열성능 평가방법의 적합성을 확인하였다.

• 한국미생물·생명공학회지
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• 제18권3호
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• pp.260-267
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• 1990

분쇄마찰매체 함유 효소반응계에서 순수분리된 glucoamylase 또는 $\alpha$-amylase에 의한 옥수수 생전분의 효소당화 mechanism을 규명코자, 생성된 당조성, SEM을 이용한 전분입자의 구조, 효소흡착량 그리고 amylose 함량 등의 변화를 관찰하였다. 생성당 조성은 분쇄마찰매체 효소반응계에서도 큰 변화없이 glucoamylase의 경우 반응초기부터 glucose가 주로 생성되었고, $\alpha$-amylase의 경우에는 maltopentaose (G5)를 포함한 oligosaccharide(G2-G8)가 주고 생성되었고 약간의 glucose가 포함되었으며, 당조성은 경시적으로 크게 변하지 않았다. SEM으로 전분입자의 구조를 관찰한 결과, 효소를 첨가하지 않을 경우 분쇄마찰매체의 기계적 충격은 전분입자의 구조변화에 큰 영향을 미치지는 못하였고 다만 전분입자를 균열시켰다.

• The Plant Pathology Journal
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• 제32권2호
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• pp.136-144
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• 2016

Pseudomonas fluorescens pc78 is an effective biocontrol agent for soil-borne fungal diseases. We previously constructed a P43-gfp tagged biocontrol bacteria P. fluorescens pc78-48 to investigate bacterial traits in natural ecosystem and the environmental risk of genetically modified biocontrol bacteria in tomato rhizosphere. Fluctuation of culturable bacteria profile, microbial community structure, and potential horizontal gene transfer was investigated over time after the bacteria treatment to the tomato rhizosphere. Tagged gene transfer to other organisms such as tomato plants and bacteria cultured on various media was examined by polymerase chain reaction, using gene specific primers. Transfer of chromosomally integrated P43-gfp from pc78 to other organisms was not apparent. Population and colony types of culturable bacteria were not significantly affected by the introduction of P. fluorescens pc78 or pc78-48 into tomato rhizosphere. Additionally, terminal restriction fragment length polymorphism profiles were investigated to estimate the influence on the microbial community structure in tomato rhizosphere between non-treated and pc78-48-treated samples. Interestingly, rhizosphere soil treated with strain pc78-48 exhibited a significantly different bacterial community structure compared to that of non-treated rhizosphere soil. Our results suggest that biocontrol bacteria treatment influences microbial community in tomato rhizosphere, while the chromosomally modified biocontrol bacteria may not pose any specific environmental risk in terms of gene transfer.

• 한국추진공학회지
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• 제18권5호
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• pp.1-11
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• 2014

하나의 탄약이 폭발하였을 경우 에너지가 다른 탄약으로 전달되어 폭발을 야기해 최종적으로 연쇄적인 폭발에 이를 수 있다. 본 연구에서는 PBXN-109가 충전된 155 mm 고폭탄의 동조폭발에 대한 2차원 하이드로 시뮬레이션을 수행하여 여폭약과 수폭약간 거리 및 중간 완충제에 따른 순폭 여부를 예측하였다. 계산 결과, 가까운 거리의 화약 간 순폭은 충격 점화로 인해 발생하며, 상대적으로 먼 거리의 경우에는 케이스의 파손으로 발생한 파편과의 충돌이 주요 원인으로 나타났다. 완충제는 폭굉파와 파편의 효과를 억제하여 동조폭발의 발생 확률을 낮출 수 있는 것으로 예측되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권7호
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• pp.922-926
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• 2006

A genetic polymorphism study on butyrophilin gene was carried out to explore variability of this gene and to estimate effects of such variability on milk quality traits in crossbred cattle. Polymorphism was unraveled by conducting Hae III PCR-RFLP of this gene. Three genotypes such as AA, BB and AB and two alleles namely A and B were observed in crossbred population. The frequencies of genotypes and alleles were 0.78, 0.17 and 0.04 for AA, AB and BB genotypes, respectively, and 0.87 and 0.13 for A and B alleles, respectively. The nucleotides, which have been substituted from allele A to B, were observed as C to G ($71^{st}$ nucleotide), C to T ($86^{th}$ nucleotide), A to T ($217^{th}$ nucleotide), G to A ($258^{th}$ nucleotide), A to C ($371^{st}$ nucleotide) and C to T ($377^{th}$ nucleotide). The nucleotide substitutions at $71^{st}$, $86^{th}$ and $377^{th}$ position of the fragment were found as silent mutations whereas nucleotide changes at $217^{th}$, $258^{th}$ and $371^{st}$ positions were detected as substitution of amino acid lysine with arginine, valine with isoleucine, and leucine with proline from allele A to B. The genotypes had significant effects ($p{\leq}0.05$) on total milk solid%, fat%, SNF%, while showing nonsignificant impact on total protein%. AA genotype produced highest average yield for all the traits.

• Composites Research
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• 제24권6호
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• pp.7-11
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• 2011

우레탄 수지 첨가에 의한 다이니마/비닐에스터 복합재료의 방탄효과 향상을 고찰하였다. 첫째로 우레탄 수지와 비닐에스터 수지를 스프레이 코팅하여 프리프레그를 제조하였으며, 수지함량을 21%로 조절하였다. 또한 혼합수지를 스프레이 코팅과 필름코팅으로 비교를 위한 프리프레그를 제조하였다. 그 다음 이들을 상온에서 1-2시간, 그리고 필요시 $100^{\circ}C$ 30분 정도 건조시켰다. 이들 프리프레그를 24층 적층한 다음 $120^{\circ}C$에서 5분 동안 정치 시킨 후 25분 동안 150 $kg/cm^2$ 압력하에서 경화 시켰다. 모의 파편탄(17 gr)을 이용하여 이들 복합재료의 방탄성능($V_{50}$)를 측정하였으며, UP127 및 EB8200으로 제조된 시편은 XSR10으로 제조된 시편에 비하여 각각 6.5 및 9.0 % 향상된 값을 보였다.