• Archives of Pharmacal Research
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• 제21권5호
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• pp.543-548
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• 1998

We examined the immunological properties of the recombinant hepatitis B surface antigen (r-HBsAg) which was expressed in mammalian cell (C127). The cross-immunity of r-HBsAg and plasma-derived hepatitis B surface antigen (p-HBsAg) were tested using Western blotting and ELISA with guinea pig polyclonal antibody and naturally infected human-derived antibody and the both antigens show the same results in their response pattern and intensity, which indicate they have a good cross-immunity. from the measurement of $ED_{50}$ after formalin- or heat-inactivation, both r-HBsAg and p-HBsAg and p-HBsAg showed $ED_{50}$ of 0.2-0.3 in formalin-inactivaton, while r-HBsAg was 0.05-0.09 and p-HBsAg was 0.03-0.07 in heat-inactivation, which means heat-inactivation method is 3-4 times superior in immunogenicity. In the immunopersistency test performed in guinea pig for the period of 3 months with two different adjuvants, antibody titer was 34.2 with muramyl dipeptide adjuvant, which was 1.8 times greater than the antibody titer of 18.9 with $AIPO_{4}$ adjuvant. the mutagenicity of r-HBsAg has the same cross-immunity with p-HBsAg, and heat-inactivation method and muramyl dipeptide adjuvant allow development of r-HBsAg vaccine with excellent immunogenicity.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2006년도 International Meeting of the Microbiological Society of Korea
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• pp.20-21
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• 2006

• BMB Reports
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• 제34권5호
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• pp.408-414
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• 2001

Ornithine decarboxylase (EC 4.1.1.17) is an essential enzyme for polyamine synthesis and growth in mammalian cells and plants. We compared the biochemical and immunological properties of rat and Nicotiana glutinosa ODC by cloning and expressing the recombinant proteins. The primary amino acid sequence between rat and N. glutinosa ODC had a 40% homology The molecular weight of the overexpressed rat ODC was 53 kDa, and that of N. glutinosa was 46.5 kDa. Adding 1 mM of putrescine to the enzyme reaction mixture inhibited both rat and N. glutinosa ODC activity to 30%. Agmatine had an inhibitory effect only on N. glutinosa ODC. Cysteine and lysine modifying reagents reduced both ODC activities, verifying the key roles of cysteine and lysine residues in the catalytic mechanism of ODC. ELISA was performed to characterize the immunological difference between the rat and plant ODC. Both the rat and N. glutinosa ODC were recognized by the polyclonal antibody that was raised against purified N. glutinosa ODC, but the rat ODC was 50-fold less sensitive to the antibody binding. These results indicate that even though both ODCs have the same evolutionary origin, there seems to be a structural distinction between the species.

• 한국동물학회지
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• 제34권1호
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• pp.1-7
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• 1991

Lipophorin of Lymuntrn'adispor L. has been purified by KBr density gradient ultracentrifugation. The properties and synthetic site of lipophorin and quantitative change of lipophorin during development have been determined using electrophoresis and immunological analysis. Lipophorin is composed of ho subunits. apo-Lpl (230.000), ago-Lpll (49,000). ann contains carbohydrates and lipids. Anti-lipophorin showed positive reactions with fat body extract and ovary extract but not with gut extract. The concentration of lipophorin in hemolymph showed gradual decrease during larval and pupal stages. Also. fat body released lipophorin into medium. Immunological test showed some partial identity between lipophorin of Lymantpia dispar and hemolymph proteins (probably lipophorin) of Hyphontria cuneo and Galleria metlonefla.

• 한국잠사곤충학회지
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• 제30권2호
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• pp.96-104
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• 1988

누에 난황단백질의 주성분인 난황소(vitellin)를 분리하고 그 면역반응적 특성과 난소 이식과의 관계 및 배발생중의 양적 변화를 조사한 결과를 요약하면 다음과 같다. 1. 누에의 용혈액 vitellogenin과 난황소는 원심분리에 이은 DEAE-cellulose column chromatography에 의해 거의 순수하게 분리되었으며 두 단백질의 polyacrylamide gel 전기영동상의 이동도는 같았다. 2. 누에의 난황소와 이의 전구물질인 용혈림프 vitellogenin은 면역반응적으로 서로 동질임이 확인되었고, 가잠의 근록곤충인 상잠(Bombyx mandarina)의 난황단백질에는 가잠의 난황소와 면역학적으로 같은 반응을 나타낸 항원이 있는 반면, 천잠(Antheraea yamamai)의 난황단백질에는 가잠과의 공통항원이 없었다. 3. 수누에에 이식한 난소에서 생성된 난황단백질에는 난황소가 결여됨이 확인되어 난황소의 합성에는 난소이외의 다른 암컷 기관에서 생성된 전구물질이 필요함을 나타내었다. 4. 배발생중의 난황소는 배자발육 후기에 주로 기관의 형성에 이용되어 배자발육 초기부터 꾸준하게 이용되는 난특이단백질(ESP)과는 이용상 다른 특성을 보였다.

• Tissue Engineering and Regenerative Medicine
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• 제15권6호
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• pp.771-779
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• 2018

BACKGROUND: Mesenchymal stromal cells (MSCs) are multipotent stem cells that can differentiate into several cell types. In addition, many studies have shown that MSCs modulate the immune response. However, little information is currently available regarding the maintenance of immunomodulatory characteristics of MSCs through passages. Therefore, we investigated and compared cytokine and gene expression levels from adipose (AD) and bone marrow (BM)-derived MSCs relevant to immune modulation from early to late passages. METHODS: MSC immunophenotype, growth characteristics, cytokine expressions, and gene expressions were analyzed. RESULTS: AD-MSCs and BM-MSCs had similar cell morphologies and surface marker expressions from passage 4 to passage 10. Cytokines secreted by AD-MSCs and BM-MSCs were similar from early to late passages. AD-MSCs and BM-MSCs showed similar immunomodulatory properties in terms of cytokine secretion levels. However, the gene expressions of tumor necrosis factor-stimulated gene (TSG)-6 and human leukocyte antigen (HLA)-G were decreased and gene expressions of galectin-1 and -3 were increased in both AD- and BM-MSCs with repeated passages. CONCLUSION: Our study showed that the immunophenotype and expression of immunomodulation-related cytokines of AD-MSCs and BM-MSCs immunomodulation through the passages were not significantly different, even though the gene expressions of both MSCs were different.

• 대한전기학회논문지
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• 제41권9호
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• pp.1104-1109
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• 1992

A mathematical model of cancerous system based on immunological surveillance has been proposed by Lee. The model involves a system of 12 coupled nonlinear differential equations due to cellular kinetics and each of them can be modeled bilinearly. This paper discusses only the properties of solutions to the nonlinear differential equations and identification.

• Journal of Ginseng Research
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• 제27권3호
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• pp.115-119
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• 2003

There has been continuing interest in the development of synthetic and natural compounds that modify the immune response particularly for the treatment of AIDS and cancer. During the past fifty years, numerous scientific studies have been published on ginseng. Modem human studies have investigated preventive effect of ginseng on several kinds of cancer, its long term immunological effect on HIV patients, its effect on cell mediated immune functions in healthy volunteers. Similarly non clinical studies on animal model system have studied the chemopreventive action of ginseng on cancer and immunological properties of ginseng. The precise mechanism of action of ginseng, however, not clearly understood. Considering its wide-ranging therapeutic effects, this study is being undertaken to elucidate the general mode of action of ginseng, especially to test our hypothesis that its biological action may be mediated by the immune system.

• 미생물학회지
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• 제13권3호
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• pp.91-100
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• 1975

The chemolithoautotrophic bacteria, Freeobacillus ferroxidans and Thiobacillus thiooxidans were identified according to their immunological and serological properties. The antibody to these organisms was easily elicited in experimental animals, however, the overall serologicl reactivities were low according to different kinds of titration methods. By means of the quantitive and qualitative analysis such as hemagglutination or ouchterlony tests, F.ferroxidans and T. thiooxidans were different in their immunoreactivities, whereas the strains among the F.ferrooxidans were possessed, in some extent, the sharing antigenic determinants. In the results of the polyacrylamide gel electrophoresis/radioimmunometric method, the major antigenic determinants of the organisms illustrated the type specificitles in the fraction number of 20-30 in their gel electrophoretograms with some modifications of the antigenic moieties.

• 한국자원식물학회지
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• 제30권6호
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• pp.662-669
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• 2017

Rubia cordifolia has been used to treat diseases for many years in China and India. Although the biological properties and major compounds of R. cordifolia have been extensively studied, the underlying mechanisms of its biological effects remain elusive. In terms of immunological effects, anti-inflammation effect of macrophage (Raw 264.7) simply has been reported. In this study, R. cordifolia was extracted in 70% ethanol and the extract did not affect to macrophage (Raw 264.7) pro-inflammation and T cell (Molt-4). However, in mast cell (RBL-2H3), it showed inhibition of degranulation. The inducing inhibitory effect on degranulation was related to concentration dependent variation in phosphorylation of ERK-1/2 and upregulating the JNK phosphorylation in RBL-2H3 cells. Based on these data, we concluded that R. cordifolia newly have anti-allergenic effects in RBL-2H3 and might be used as a therapeutic agent to treat or prevent allergic diseases such as asthma and atopic dermatitis.