• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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• pp.195-195
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• 1998

Ginkgetin was previously reported as an inhibitor of group II phospholipase A$_2$. It also inhibited in vitro arachidonate release from the activated macrophages and lymphocyte proliferation. These previous studies suggested an anti-inflammatory nature of ginkgetin, especially on chronic inflammation. In fact, ginkgetin showed potent anti-inflammatory activity against rat adjuvant-induced arthritis, a chronic inflammatory animal model, with comparable analgesic activity. In order to investigate the action mechanisms, tumor necrosis factor and interferone release were studied from human PMMC. It was found that ginkgetin clearly inhibited release of these cytoknes from human PMMC. Ginkgetin was also found to inhibit immunoglobulin M production at 1 - 10 uM. These results may contribute to antiarthritic activity of ginkgetin in vivo.

• Parasites, Hosts and Diseases
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• 제40권2호
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• pp.93-99
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• 2002

The effect of a secretory proteinase from the pathogenic amoebae Acanthamoeba castellanii on host's defense-oriented or regulatory proteins such as immunoglobulins, interleukin-1, and protease inhibitors was investigated. The enzyme was found to degrade secretory immunoglobulin A (slgA), IgG, and IgM. It also degraded $interleukin-1{\alpha}$ ($IL-l{\alpha}$) and $IL-l{\beta}$. Its activity was not inhibited by endogenous protease inhibitors, such as ${\alpha}$2-macroglobulin, ${\alpha}l-trypsin$ inhibitor, and ${\alpha}2-antiplasmin$. Furthermore, the enzyme rapidly degraded those endogenous protease inhibitors as well. The degradation of host's defense-oriented or regulatory proteins by the Acanthanoeba proteinase suggested that the enzyme might be an important virulence factor in the pathogenesis of Acanthamoeba infection.

• 분석과학
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• 제30권1호
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• pp.49-56
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• 2017

Here, we demonstrate surface functionalization of Au chips with 4-(carboxymethyl)aniline (CMA) and amine-terminated polyamidoamine (PAMAM) dendrimers for immobilization of antibodies on the Au surfaces. Use of the functionalization strategy led to high surface density of the immobilized antibodies on the Au chips. Specifically, we found that the functionalization of Au chips with CMA and amine-terminated $6^{th}$ generation PAMAM dendrimers allowed immobilization of immunoglobulin (IgG) antibodies with high surface density, which is 5 times higher than that obtained with Au surfaces functionalized with CMA and ethylenediamine.

• EDISON SW 활용 경진대회 논문집
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• 제6회(2017년)
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• pp.715-718
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• 2017

많은 항바이러스제의 개발에도 불구하고, B형간염바이러스 감염으로 인해 간 이식을 받은 환자를 대상으로 한 재발 억제 치료 시 항 B형간염바이러스 면역글로불린(Anti-hepatitis B immunoglobulin, HBIG) 치료는 아직도 가장 중요한 약물요법으로 여겨지고 있다. 본 연구진은 간 이식 환자에서의 6개월 간의 약동학 연구를 통해, HBIG의 약동학적 파라미터와 이에 대한 영향인자를 확인하였으며, 이를 이용해 용법용량 별로 유지요법 시기에 목표 농도 도달 가능성을 예측하는 시뮬레이션 tool을 개발하였다. 그 결과 95% 환자가 목표 농도 이상의 HBIG 농도를 유지하기 위한 용량은 '목표 농도 ${\times}$ 20'에 해당하는 것을 확인하였으며, 다수의 대학병원에서 통상적인 HBIG 유지 요법의 목표 농도를 최소 300 IU/L로 하고 있음을 고려할 때, 6,000 IU의 용량을 월 1회 투여하는 것이 바람직할 것으로 판단하였다.

• 대한임상독성학회지
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• 제16권1호
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• pp.57-59
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• 2018

Severe systemic responses including neurologic complications such as myasthenia gravis, myeloradiculopathy, optic neuropathy, parkinsonism, stroke and Guillain-$barr{\acute{e}}$ syndrome can occur after bee stings. This case describes a 78-year-old female who presented with symptoms of acute progressive bilateral symmetrical weakness in both lower legs after multiple bee stings. Nerve conduction study findings were consistent with acute sensorimotor axonal neuropathy and recovered by treatment with intravenous immunoglobulin. This case highlights that bee stings can result in acute onset Guillain-$barr{\acute{e}}$ syndrome, although the pathophysiologies of bee venoms need to be investigated accurately.

• 약학회지
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• 제47권3호
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• pp.190-194
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• 2003

As previously reported, an immunoglobulin M (IgM) monoclonal antibody (MAb) B6.1, specific for a cell wall B-l,2-mannotriose, was protective against vaginal infection due to Candida albicans when mice were treated with the antibody. In this study, the role of neutrophil was examined in the protective effect of MAb B6.1 against vaginal infection. To deplete neutrophils, mice were given intravenously rat anti-mouse neutrophile MAb RB6-8C5 prior to intraperitoneal administration of MAb B6.1 to these mice. The mice were examined for antibody in their reproductive tract. By an ELISA, MAb B6.1 was found in the vaginal homogenates, but no antibody was detected in vaginal lavage materials. The neutropenia was induced by a single dose of the anti-neutrophil antibody, but lymphocytes were also partially depleted. The protective effect of MAb B6.1 was decreased when mice pretreated with MAb RB6-8C5 were given the anti-Candida antibody before challenge with C. albicans yeast cells intravaginally. These results show that neutrophils are involved in the MAb B6.1 protection against Candida vaginal infection.

• 한국막학회:학술대회논문집
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• 한국막학회 1994년도 추계 총회 및 학술발표회
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• pp.72-73
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• 1994

Whey는 일명 lactoserum 이라고도 하며 치즈제조시 우유를 응고시키는 과정에서 Casein과 지방으로부터 분리되어 나오는 액상의 부산물로 본래 우유 부피의 약 90%를 차지하며, 용해성 단백질, 유당, 비타민과 무기질 등을 함유하고 있다. 유청에 함유되어 있는 단백질은 건조고형분의 약 13%가 되는데, 주요 단백질은 $\beta$-lactoglobulin(50%), $\alpha$-lactalbumin(22%), Serum albumin(5%), Immunoglobulin(12%), Proteose-peptone(10%) 등이 있다. 유청단백질중 가장 많이 함유되어 있는 $\beta$-lactoglobulin은 구형의 단백질로 단량체의 분자량은 약 18,400이며, pH 3.5~7 범위내에서는 해리되지 않는 이량체(dimer)를 형성한다. pH 3.5 이하에서 이량체는 해리되고 다량체의 형성으로 재평성한다. pH 7.0 이상의 알칼리 영역에서는 Conformational Change가 일어나는 것으로 알려져 있으며, 등전점(isoelectric point)은 pH 5.2이다. $\alpha$-lactalbumin은 14,200의 분자량을 가지는 구형의 단백질로 등전점은 pH 4.8이다.

• Clinical and Experimental Pediatrics
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• 제53권9호
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• pp.855-858
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• 2010

Kawasaki disease (KD) causes multisystemic vasculitis but infrequently manifests with deep neck infections, such as a peritonsillar abscess, peritonsillar or deep neck cellulitis, suppurative parapharyngeal infection, or retropharyngeal abscess. As its etiology is still unknown, the diagnosis is usually made based on typical symptoms. The differential diagnosis between KD and deep neck infections is important, considering the variable head and neck manifestations of KD. There are several reports on KD patients who were initially diagnosed with retropharyngeal abscess on on computed tomography scans (CT). However, the previously reported cases did not have abscess or fluid collection on retropharyngeal aspiration. Therefore, false-positive neck CT scans have been obtained, until recently. In this case, suspected neck abscess in patients with KD unresponsive to intravenous immunoglobulin could signal the possible coexistence of suppurative cervical lymphadenitis.

• Bulletin of the Korean Chemical Society
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• 제27권4호
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• pp.479-483
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• 2006

We developed a microfluidic immunoassay platform for the detection of various analytes such as bacterial pathogen by packing antibody-immobilized glass beads in spatially-isolated microchambers on a microfluidic device. Primary amines of antibody were covalently conjugated to carboxyl-terminated glass beads previously treated with aminosilane followed by glutaraldehyde. Through this covalent binding, up to 905 $\mu$g immunoglobulin G (IgG) per gram of glass beads was immobilized. For application, glass beads attaching antibody specific to Escherichia coli O157:H7, a foodborne pathogen, were packed into a microfluidic device and used for the detection of the serotype. This prototype immunoassay device can be used for the simultaneous detection of multiple analytes by sequentially packing different-sized glass beads attaching different antibody in discrete microchambers on a single microfluidic device.

• Annals of Clinical Neurophysiology
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• 제22권2호
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• pp.117-120
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• 2020

Miller Fisher syndrome (MFS) is characterized by the acute ophthalmoparesis, ataxia and areflexia. We describe the case of 70-year-old man with cardinal symptom of MFS and active pulmonary tuberculosis (Tb). A thorough evaluation led to the diagnosis of MFS and treatment with intravenous immunoglobulin (IVIg) was started. The complete resolution of ophthalmoparesis and ataxia was observed from the fourth day of IVIg treatment. This is the first report to describe a case of MFS that developed in patient pulmonary tuberculosis.