[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5012/bkcs.2006.27.4.479

Microfluidic Immunoassay Platform Using Antibody-immobilized Glass Beads and Its Application for Detection of Escherichia coli O157:H7

Lee, Nae-Yoon (Division of Nano Sciences, Ewha Womans University)
Yang, Yoon-sun (Division of Nano Sciences, Ewha Womans University)
Kim, Youn-Sang (Division of Nano Sciences, Ewha Womans University)
Park, Sung-su (Division of Nano Sciences, Ewha Womans University)

Publication Information

Bulletin of the Korean Chemical Society / v.27, no.4, 2006 , pp. 479-483 More about this Journal

Abstract

We developed a microfluidic immunoassay platform for the detection of various analytes such as bacterial pathogen by packing antibody-immobilized glass beads in spatially-isolated microchambers on a microfluidic device. Primary amines of antibody were covalently conjugated to carboxyl-terminated glass beads previously treated with aminosilane followed by glutaraldehyde. Through this covalent binding, up to 905 $\mu$ g immunoglobulin G (IgG) per gram of glass beads was immobilized. For application, glass beads attaching antibody specific to Escherichia coli O157:H7, a foodborne pathogen, were packed into a microfluidic device and used for the detection of the serotype. This prototype immunoassay device can be used for the simultaneous detection of multiple analytes by sequentially packing different-sized glass beads attaching different antibody in discrete microchambers on a single microfluidic device.

Keywords

Microchamber; Glass bead; IgG immobilization; Pathogenic bacteria; Escherichia coli O157:H7;

Citations & Related Records

Times Cited By Web Of Science : 6 (Related Records In Web of Science)
Times Cited By SCOPUS : 5

Reference
Cited By SCOPUS

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1	/ Current Proteomics
2	/ Journal of Agricultural and Food Chemistry
3	/ Environmental Technology
4	/
5	/ JALA - Journal of the Association for Laboratory Automation

1	Nanoscale Characterization of Escherichia coli Biofilm Formed under Laminar Flow Using Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM) / [Lim, Jee-Sun;Lee, Kang-Mu;Kim, So-Hyun;Nam, Seong-Won;Oh, Yoo-Jin;Yun, Hyun-Sun;Jo, William;Oh, Se-Jong;Kim, Sae-Hun;Park, Sungsu;] / Bulletin of the Korean Chemical Society
2	A Microfluidic Platform for Preconcentrating and Detecting Cu(II) with a Fluorescent Chemosensor and Cu(II)-Chelating Alginate Beads / [Kim, Jeong-A;Hwang, Hye-Jin;Jun, Eun-Jin;Nam, Seong-Won;Lee, Kang-Mu;Kim, So-Hyun;Yoon, Ju-Young;Kang, Suk-Won;Park, Sung-Su;] / Bulletin of the Korean Chemical Society
3	Continuous Production of Immunoliposomes using a Microvalve-controlled Microfluidic Device (μFD) / [Jin, Yan;Kim, So Hyun;Kim, Myunghee;Park, Sungsu;] / Bulletin of the Korean Chemical Society