• 한국미생물·생명공학회지
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• 제47권4호
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• pp.509-514
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• 2019

본 연구에서는 제주연안에 서식하는 감태에서 폴리페놀을 추출하고 남은 가공부산물을 이용하여 가축의 생산성 및 면역력을 증진할 수 있는 소재를 개발하고자 하였다. 흰쥐를 이용하여 감태 가공부산물을 0.1% 첨가 급여한 그룹에서 체중 증가량과 맹장 내 Lactobacillus spp.의 유의한 증가가 확인되었다. 또한, 혈액 분석 결과 일반적 혈액분석항목에서는 변화가 없었으나, IgA와 IgG 항목에서 유의한 증가가 나타났다. 흰쥐의 전체적인 체중 증가와 더불어 비복근을 절개하여 근육 성장 관련 유전자 발현을 분석한 결과, MyoD, Myogenin, IGF-1의 유의한 증가가 확인되었다. 감태 가공부산물 0.1% 급여 효과가 면역력을 증진하고, 근육성장에 기여하여 체중증가에 직접적으로 영향을 준 것임을 시사한다. 최종적으로 감태 가공부산물을 폐기하지 않고 성분 분석 및 효능 평가를 통해 생산성 증가용 기능성 사료첨가제로의 가능성을 확인하였으며, 향후 양계, 양돈 등 다양한 축종을 대상으로 생산성 평가 및 생리기능 평가가 수반되어야 할 것이다.

• 한국임상약학회지
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• 제27권3호
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• pp.127-135
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• 2017

Background: Kawasaki disease (KD) is an acute febrile, systemic vasculitis as a leading cause of acquired heart disease in children. Intravenous immunoglobulin G (IVIG) and aspirin are the standard initial therapy in the treatment of acute KD. The purpose of this study was to investigate drug utilization in children with KD, and to compare "IVIG + high-dose aspirin" and "IVIG + moderate-dose aspirin" in preventing cardiac complications. Methods: We analyzed pediatric patient sample data compiled by the Health Insurance Review & Assessment Service from 2010 to 2015. We identified patients with KD using the KCD-6 code of M30.3. We excluded patients in chronic phase or ${\geq}1$0 years. We also excluded patients who were diagnosed KD in November or December. Drug utilization pattern were assessed in acute KD patients and 30-day and 60-day cardiac complications were investigated between "IVIG + high-dose aspirin" group and "IVIG + moderate-dose aspirin" group. Results: In acute phase, IVIG was administered to 95.8% patients, and 57.1% patients were prescribed moderate-dose aspirin and 25% patients were with high-dose aspirin. Steroid use was rapidly increased from 4.0% in 2010 to 11.3% in 2015. Both 30-day and 60-day cardiac complications occurred less in "IVIG + high-dose aspirin" group compared to "IVIG + moderate-dose aspirin" group, but not statistically significant (0.9% vs 1.8%, p=0.252 for 30-day complication rate; 1.5% vs 2.7%. p=0.073 for 60-day complication rate). Conclusion: We were not able to demonstrate which aspirin therapy is superior for preventing cardiac complications in acute KD patients and further research is warranted.

• 대한예방한의학회지
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• 제15권3호
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• pp.163-178
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• 2011

Objective : This study is conducted to evaluate the efficiency of ethanol extracts of 4 mixed herbs(CP001 or CP002) on mouse model of atopic dermatitis induced by ovalbumin. Methods : Female BALB/c mice were internally sensitized with ovalbumin($20{\mu}g$) plus aluminum hydroxide hydrate(4mg) once per week. After 3 weeks, they were dermally challenged with patches containing ovalbumin ($100{\mu}g$) plus aluminum hydroxide hydrate(20mg) every other day for 3 weeks. After induction of atopic dermatitis, mice back skin were gently rubbed with CP001 or CP002(200mg/$m{\ell}$, $100{\mu}{\ell}$) for two weeks(every 2 days). Results : In CP001 or CP002 treated group, there was a remarkable reduction in infiltration of eosinophils on the skin areas and diminution of mast cells and total T cells in blood samples as compared with control group. Cutaneous expressions of interleukin-13, 17 were also decreased by CP001 or CP002. Moreover, blood immunoglobulin E level was decreased by drug administration while there was no decrease in OVA sensitization group. Conclusion : In summary, our result shows that herbal extracts(CP001 and CP002) could be potential candidates for the treatment of chronic atopic dermatitis.

• Clinical and Experimental Pediatrics
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• 제57권9호
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• pp.396-402
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• 2014

Purpose: The introduction of the mumps vaccine has dramatically reduced the number of mumps cases, but outbreaks have recently occurred among highly vaccinated populations in developed countries. Epidemiological and clinical characteristics of patients with mumps admitted between 1989 and 2012 in a single hospital in Korea are described in the present study. Methods: We retrospectively evaluated inpatients with mumps between 1989 and 2012 and outpatients and inpatients with mumps in 2011-2012. Results: A total of 152 patients with mumps were admitted between 1989 and 2012, and 163 patients were recorded in 2011-2012. The highest number of admitted cases occurred in 1998 and 2012 (35 and 34 cases, respectively). Among the patients admitted in 2011-2012, the highest frequency was observed among people aged 15-19 years, and low frequency was observed in those aged <4 years and >20 years, compatible to the city data and national data. In patients admitted to our department in 1998 (35 cases) and in 2010-2012 (27 cases), there were significant differences in the mean age and the rate of secondary measles-mumps-rubella (MMR) vaccination, but had similar clinical features, including complications, except aseptic meningitis. Antimumps immunoglobulin (Ig) G was positive in 83% and 100%, and IgM was positive in 67% and 41%, respectively, in the two periods. Conclusion: In Korea, recent mumps outbreaks have occurred mainly among secondary school students who received two doses of the MMR vaccine. The vaccinees might have a modified immune reaction to viral insults, manifesting modified epidemiological and clinical features.

• Clinical and Experimental Pediatrics
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• 제59권4호
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• pp.178-182
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• 2016

Purpose: Medium-dose (1 g/kg) intravenous immunoglobulin (IVIG) is effective in the majority of patients with Kawasaki disease (KD) but some patients who do not respond to medium-dose IVIG are at high risk for the development of coronary artery lesions (CALs). The purpose of this study was to identify the clinical predictors associated with unresponsiveness to medium-dose IVIG and the development of CALs. Methods: A retrospective study was performed in 91 children with KD who were treated with mediumdose IVIG at our institution from January 2004 to December 2013. We classified the patients into responders (group 1; n=68) and nonresponders (group 2; n=23). We compared demographic, laboratory, and echocardiographic data between the 2 groups. Results: Multivariate logistic regression analysis identified 6 variables as predictors for resistance to medium-dose IVIG. We generated a predictive scoring system assigning 1 point each for percentage of neutrophils ${\geq}65%$, C-reactive protein ${\geq}100mg/L$, aspartate aminotransferase ${\geq}100IU/L$, and alanine aminotransferase ${\geq}100IU/L$, as well as 2 points for less than 5 days of illness, and serum sodium level ${\leq}136mmol/L$. Using a cutoff point of ${\geq}4$ with this scoring system, we could predict nonresponsiveness to medium-dose IVIG with 74% sensitivity and 71% specificity. Conclusion: If a patient has a low-risk score in this system, medium-dose IVIG can be recommended as the initial treatment. Through this process, we can minimize the adverse effects of high-dose IVIG and incidence of CALs.

• Journal of Animal Science and Technology
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• 제47권1호
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• pp.19-28
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• 2005

The objectives of the current study were to develop polyclonal antibodies in sheep against adipocyte plasma membrane(APM) proteins isolated from swine, to investigate tissue specificity, and to determine cytotoxic effects of antiserum on swine adipocytes. Plasma membrane proteins from adipocyte, brain, heart, kidney, liver, and spleen were isolated using a 32% sucrose gradient. Adult male sheep was immunized three times at three week interval with the purified swine APM proteins. Antiserum was taken from immunized sheep at 10, 12, and 14 days after the third immunization. Antiserum expressed strong reactivity with APM proteins determined by enzyme-linked immunosorbent assay(ELISA), and the reactivity could be detected at dilutions in excess of 1 : 81,000. Antiserum showed very low binding affinity with proteins isolated from brain, heart, kidney, liver, or spleen. Tissue specificity of the antiserum was reconfirmed by Western immunoblotting using anti-sheep immunoglobulin G•alkalinephosphatase conjugate as a secondary antibody. The reactivity of antiserum to the external surface of fixed swine adipocytes was confmned by an immunohistochemical technique using anti-sheep immunoglobulin G-FITC. Confluent swine adipocytes in culture were lysed by antiserum treatment and cytosolie lactate dehydrogenase(LDH) was released as a dose-dependent patterns while adipocytes treated with normal sheep serum maintained their integrity and expressed low level of LDH. These results implicate that fat contents in the pigs can be reduced by immunological methods.

• 한국자기공명학회논문지
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• 제20권3호
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• pp.95-101
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• 2016

Apolipoprotein B-100 (Apo-B100) is a major component of low density lipoprotein (LDL). Apo B-100 protein has 4,536 amino acid sequence and these amino acids are classified into peptide groups A to G with subsequent 20 amino acids (P1-P302). The peptide groups were act as immunoglobulin (Ig) antigens which oxidized via malondialdehyde (MDA). The mimetic peptide P1 (EEEMLENVSLVCPKDAT RFK) out of D-group peptides carrying the highest value of IgG antigens were selected for structural studies that may provide antigen specificity. Circular Dichroism (CD) spectra were measured for peptide secondary structure in the range of 190-250 nm. Experimental results show that P1 exhibit partial of ${\beta}-sheet$ and random coil structure. Homonuclear (COSY, TOCSY, NOESY) 2D-NMR experiments were carried out for NMR signal assignments and structure determination for P1. On the basis of these completely assigned NMR spectra and distance data, distance geometry (DG) and Molecular dynamics (MD) were carried out to determine the structures of P1. The proposed structure was selected by comparisons between experimental NOE spectra and back calculated 2D NOE results from determined structure showing acceptable agreement. The total Root-Mean-Square-Deviation (RMSD) value of P1 obtained upon superposition of all atoms was in the range $0.33{\AA}$. The solution state P1 has mixed structure of ${\beta}-sheet$ (Glu[1] to Cys[12]) and random coil (Pro[13] to Lys[20]). These NMR results are well consistent with secondary structure from experimental results of circular dichroism. Structural studies based on NMR may contribute to the studies of atherosclerosis and observed conformational characteristics of apo B-100 in LDL using monoclonal antibodies.

• 동의생리병리학회지
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• 제34권5호
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• pp.245-254
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• 2020

The purpose of this study was to investigate the effects of natural herb mixture containing Laminaria japonica Areschoung (LAM) on fine dust-induced bronchitis in mice. Laminaria japonica Areschoung is main content of LAM, which is including fucoidan. Fucoidan extracted from phaophyta is known to prevent bronchitis and to defend against bacteria and virus infection. In this study, we experienced the effect of LAM on bronchitis and investigated gene expressions (e.g ; IL-8, COX-2, MCP-1) and bio-markers (e.g ; IL-8, PGE2, MUC5AC) associated with bronchitis by using A549 cells. Also, we investigated whether LAM can suppress the bronchitis in fine dust-induced animal models. We injected fine dust (50 ㎕) twice as INT (Intra-Nasal-Trachea) method. Then LAM (200 mg/kg and 400 mg/kg) were oral administered for 14 days. We analyzed the number of immune cells, immunoglobulin E, bio-markers level associated with bronchitis. LAM significantly decreased bio-marker (IL-1β, IL-6, IL-8, TNF-α, Histamine, PGE2), immune cells (white blood cell, neutrophil, lymphocyte, monocyte), and immunoglobulin E, that are increased by fine dust. Taken together, this study suggest that LAM can be used as effective herbal extract for bronchitis.

• 한국식품영양과학회지
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• 제33권2호
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• pp.271-277
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• 2004

최근에 방사선 조사에 대해 방호효과를 가지는 것으로 알려진 보중익기탕의 골수세포 분화 유도 효과에 대해 관찰하였다. 시험관에서 골수세포를 배양했을 때, 배양시간(5일,10일)에 따라 대조군의 세포 수는 현저히 감소하였고, 보중익기 탕의 total 분획을 첨가하였을 때도 비슷한 결과를 얻었다. 그러나 polysaccaride 분획을 첨가하였을 때는 감소하는 세포수가 일정한수준에서 유지되며 더 이상 감노하지 않았다. 그리고 이들 세포가 어떤 종류의 세포인지를 유세포분석기로 분석한 결과, Pre-B세포의 특징적인 세포 표면 단백질인 CDl9와 CD40을 동시에 발현한 세포인 것이 확인되었다. 또한, 이들 세포는 분화과정 이 끝난 B세포가 분비하는 IgM 뿐만 아니라 IgG1, G2a, G3를 분비하였다. 이상의 결과 보중익기탕의 polysaccaride 분획에 골수세포가 B세포로 분화 증식하는 것을 유도하는 성분이 포함되어 있어 방사선 조사로 상해를 입은 조혈계에 대한 방호 효과를 나타내는 것으로 생각된다.

• 농업과학연구
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• 제22권1호
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• pp.82-95
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• 1995

본(本) 연구(硏究)는 한국인(韓國人) 산모(産母)로 부터 수집한 초유(初乳) 및 Holstein종(種)으로 부터 수집한 초유(初乳) 중에 존재하는 Secretory immunoglobulin A의 분리방법(分離方法)을 검토하고 분리(分離)된 분획들의 면역화학적(免疫化學的) 특성을 비교 검토하여 향후 인공영양(人工營養)에 대한 시험(試驗) 자료를 얻고자 하였으며 그 결과(結果)는 다음과 같다. 1. 인유(人乳) 초유(初乳) 중의 SIgA는 Sephadex G-200과 Sepharose 6B를 통한 gel-filtration 만으로도 순수한 상태로 분리(分離) 정제할 수 있었으나, 우유(牛乳) 초유(初乳) 중의 SIgA는 gel-filtration을 반복하는 방법(方法)으로는 순수한 상태로 분리(分離) 및 정제할 수 없었으며, gel-filtration으로 얻어진 SIgA rich fraction을 모아서 DEAE를 실시한 결과(結果) 또한 마찬가지로 SIgA를 순수한 상태로 분리(分離)할 수는 없었다. 2. 인유(人乳) 초유(初乳)로 부터 gel-filtration을 통하여 분리(分離)한 분획들을 Immunoelectrophoresis 및 Double immunodiffusion 방법(方法)으로 면역화학적(免疫化學的) 특성을 살펴본 결과(結果), 첫번째 peak의 분획에서 IgM이 존재하고 있음을 확인할수 있었으며 두번째 peak의 분획에서 SIgA가 순수한 상태로 존재하고 있음을 확인할수 있었다. 반면에 우유(牛乳) 초유(初乳)로 부터 gel filtration 및 DEAE를 통하여 분리(分離)한 분획들을 같은 방법(方法)으로 면역화학적(免疫化學的) 특성을 살펴본 결과(結果), SIgA rich fraction 중에는 IgG가 다량(多量)으로 혼재(混在)되어 있음을 확인할 수 있었다. 3. 우유(牛乳) 초유(初乳)에서 분리(分離)한 SIgA rich fraction의 Fragment를 SDS-PAGE로 분자량을 측정해 본 결과(結果), Secretory component가 77,000-80,000, Heavy chain이 50,000-60,000, Light chain이 25,000-27,000 dalton으로 나타났으며 J-chain은 Light chain과 중복되어 분자량 측정이 어려웠다. 이와같은 결과(結果)는 인유(人乳) 초유(初乳) 중에 존재하는 SIgA Fragment 분자량과 유사한 결과(結果)를 보여주었다.