• 식품과학과 산업
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• 제51권1호
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• pp.72-80
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• 2018

Yogurt, which has long been consumed as a longevity food, is characterized by a large number of lactic acid bacteria. The sales of yogurt market have increased for the past three years due to research findings that the intestinal microbial environment is closely associated with many human diseases and the well-being trend of recent consumers. The recent trends in the yogurt market have focused to low sugar content, functionalities such as enhanced immunity, and yogurt for freezing. In addition, recent yogurt products tend to be labeled for certain lactic acid bacteria having functionality in their products. Many yogurts have names of specific lactic acid bacteria on their products, such as 'Activia' and 'Bifidus'. We monitored the 'Bifidus' products in the market for reflecting its regulated counting in their products required to contain more than 10 million Bifidobacteria according to the revised lactic acid bacteria-counting test of Food Code 2015.

• 한국미생물·생명공학회지
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• 제48권4호
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• pp.569-573
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• 2020

Brucellosis is a zoonotic disease caused by Brucella infections and humans usually contract this disease from close contact with infected animals or their products, usually via the ingestion of cheese or crude milk. Macrophage migration inhibitory factor (MIF) and Pro- and anti-inflammatory cytokines play an important role in susceptibility/resistance and the immunopathogenesis of Brucella infection. These cytokines are crucial factors in the initiation and progression of protective immunity against Brucella infection but the role of MIF has not been well studied in the human response to intracellular microbes. This study was designed to investigate the effect of MIF expression on Brucella susceptibility. A total of 85 positive rose Bengal tests and 24 samples from healthy individuals were collected for this study and subjected to polymerase chain reaction assays (PCR) of the bcsp31 diagnostic gene. MIF concentrations were evaluated using Enzyme-Linked immunosorbent assay (ELISA) and the results showed that 46 (54%) of the rose Bengal test samples were positive and 39 (46%) were negative for bcsp31 (p ≤ 0.05) and used as the gold standard for all of the comparisons in this study. The ELISA results indicate that the mean concentration of MIF was significantly higher in patients with positive rose Bengal tests when compared to the control groups and that its concentration increases with increasing age in both the patient and control groups (p ≤ 0.05).

• Journal of Microbiology and Biotechnology
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• 제33권1호
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• pp.123-126
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• 2023

The prevalence of candidiasis, a contagious disease with high morbidity and mortality, has sharply increased globally over the last two decades. Candida albicans can cause serious infections in patients with weak immunity and in recipients of prolonged antibiotic treatment. Consequently, rapid and accurate identification of species can play an important role in the treatment of candidiasis. Here, we investigated the positive rate and infection trend of C. albicans according to age, specimen type, and sex using multiplex real-time polymerase chain reaction-based testing of samples collected for the diagnosis of sexually transmitted diseases in Korea between 2018 and 2020. When the type of specimen collected was a swab, the positive rate of C. albicans was higher among younger women, and tended to decrease with age. Analysis of swab samples revealed higher positive rates than urinalysis. The reduction trend in positive rates by age was comparable between the overall samples and urine specimens. Among male patients, the positive rate did not differ substantially across the various types of specimens collected. Previous studies have shown a higher prevalence of non-albicans Candida species than C. albicans in clinical specimens, and exclusion of the former from our analysis may be a limitation of this study. However, our findings contribute significantly to the literature because globally, there is a paucity of epidemiological studies using molecular techniques to detect C. albicans in sexually transmitted disease test samples.

• 인간식물환경학회지
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• 제22권5호
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• pp.505-513
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• 2019

As the number of foreign workers increases in Korea, people's interest in their adaptation to this society has also increased. The purpose of this study was to investigate the effect of a forest-healing program on foreign workers' cultural adaptation stress and psychological wellbeing. The experiment was conducted in a recreation forest located in Hongcheon-gun, Gangwon-do. The subjects who participated in this study included 40 foreign workers (16 males, 24 females). The experiment was conducted for three days and two nights from June 26 to 28, 2019. The forest healing program was composed of activities that can enhance immunity and restore physical and mental health by utilizing various healing environmental factors such as landscapes, sunlight, anion and oxygen in forest. The independent sample t-test was conducted to examine changes in cultural adaptation stress and psychological well-being before and after the program. The collected data were analyzed using SPSS 18.0 program. As a result, cultural adaptation stress was statistically significantly reduced in sub-factors including discrimination experience, language conflict and legal status. Psychological wellbeing improved in all the surveyed sub-factors such as self-confidence, ego, immersion, and joy. The results of this study are expected to be used as basic data for reducing the stress foreign workers experience from cultural adaptation and restoring their psychological wellbeing in the future.

• The Plant Pathology Journal
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• 제40권2호
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• pp.218-224
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• 2024

Plants are treasure trove of novel compounds that have potential for antifungal chemicals and drugs. In our previous study, we had screened plant extracts obtained from more than eight hundred plant materials collected in Korea, and found that butanol fraction of the Actinostemma lobatum were most potent in suppressing growth of diverse fungal pathogens of plants. Here in this study, we describe further analysis of the butanol fraction, and summarize the results of subsequent antifungal activity test for the sub-fractions against a selected set of plant pathogenic fungi. This line of analyses allowed us to identify the sub-fractions that could account for a significant proportion of observed antifungal activity of initial butanol fraction from A. lobatum. Further analysis of these sub-fractions and determination of structure would provide the shortlist for novel compounds that can be a lead to new agrochemicals.

• 대한전자공학회논문지SD
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• 제46권12호
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• pp.105-112
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• 2009

본 논문에서는 $0.35{\mu}m$ Bipolar-CMOS-DMOS(BCD)공정으로 설계한 스마트 파워 IC 내의 가드링 코너 영역에서 발생하는 비정상적인 정전기 불량을 관측하고 이를 분석하였다. 칩내에서 래치업(Latch-up)방지를 위한 고전압 소자의 가드링에 연결되어 있는 Vcc단과 Vss 사이에 존재하는 기생 다이오드에서 발생한 과도한 전류 과밀 현상으로 정전기 내성 평가에서 Machine Model(MM)에서는 200V를 만족하지 못하는 불량이 발생하였다. Optical Beam Induced Resistance Charge(OBIRCH)와 Scanning Electronic Microscope(SEM)을 사용하여 불량이 발생한 지점을 확인하였고, 3D T-CAD 시뮬레이션으로 원인을 검증하였다. 시뮬레이션 결과를 통해 Local Oxidation(LOCOS)형태의 Isolation구조에서 과도한 정전기 전류가 흘렀을 때 코너영역의 형태에 따라 문제가 발생하는 것을 검증하였다. 이를 통해 정전기 내성이 개선된 가드링 코너 디자인 방법을 제안하였고 제품에 적용한 결과, MM 정전기 내성 평가에서 200V이상의 결과를 얻었다. 통계적으로 Test chip을 분석한 결과 기존의 결과 대비 20%이상 정전기 내성이 향상된 것을 확인 할 수 있었다. 이 결과를 바탕으로 BCD공정을 사용하는 칩 설계 시, 가드링 구조의 정전기 취약 지점을 Design Rule Check(DRC) 툴을 사용하여 자동으로 찾을 수 있는 설계 방법도 제안하였다. 본 연구에서 제안된 자동 검증방법을 사용하여, 동종 제품에 적용한 결과 24개의 에러를 검출하였으며, 수정 완료 제품은 동일한 정전기 불량은 발생하지 않았고 일반적인 정전기 내성 요구수준인 HBM 2000V / MM 200V를 만족하는 결과를 얻었다.

• KSBB Journal
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• 제14권2호
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• pp.241-247
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• 1999

디프테리아는 Corynebacterium diphtheriae에 의해 발생되는 호흡기 질병으로 C. diphtheriae의 exo-toxin을 불활성화 시킨 toxoid 백신을 사용하여 예방해 왔다. 현재까지 국내에서는 정치배양 방법으로 디프테리아 toxin을 생산해 왔기 때문에 생산성과 품질에 한계가 있었으며, 이를 극복하기 위해 발효조를 이용한 발효조건 최적화에 대한 연구를 수행하였다. 디프테리아 toxin을 보다 효율적을 얻기 위한 배지로서 beef antigen이 함유되어 있지 않은 casein 유래의 NZ-Case를 선택하였다. 발효조에 의한 toxin 생성은 세포성장과 함께 증가하는 growh-associated form으로 나타났다. 최대의 세포성장과 toxin 생성은 초기 pH가 7.0인 배지에서 0.22vvm의 통기와 400rpm의 교반조건에서 얻을 수 있었다. 또한 최대의 toxin 생성을 위한 최적의 iron 이온의 농도는 0.3mg/L 이었으며, morgamcphospale의 첨가에 의한 calcium-phosphate 침전이 배지 내에 iron 이온의 농도조절을 위하여 요구되었다. 면역원성을 확인하기 위한 역가시험 결과 발효조 배양에서 얻은 toxoid는 4단위에서 모든 guinea-pigs가 생존하여 2단위인 정치배양 toxin에비해 월등히 우수한 것으로 판단되었다. 결국 발효조 배양으로 toxin을 생산할 경우 부작용이 적고 수율과 생산성 및 면역원성 이 우수한 toxid의 생산이 가능하였다.

• 전기학회논문지
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• 제63권2호
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• pp.266-271
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• 2014

The Electromagnetic Interference(EMI) generating from corona discharge of transformer area can interference the digital Instrument and Control(I&C) systems located nearby transformers. When the potential gradient of the electric field around the conductor is high enough to form a conductive region but not high enough to cause electrical breakdown to nearby objects, the EMI of corona discharge emits with the conducted and radiated noise and it interferences the signals of the I&C systems. Since digital I&C systems have an efficiency and competitive price, the analog I&C systems have been upgraded and displaced with the digital I&C systems but which have less EMI Immunity. There was no assessment to I&C systems by EMI generating corona discharge nearby transformers. When the safety-related I&C systems are installed in plants, the verification of equipment EMI should be done not in site-specific test but in test facilities. There are the need to do the site-specific EMI evaluation of corona discharge nearby transformers. This paper assesses the margin between plant emission limits and the highest composite plant emission of corona. When the non safety-related I&C systems are placed in transformer area, it suggests the appropriate radiated susceptibility level to EMI of corona discharge.

• 비파괴검사학회지
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• 제28권2호
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• pp.157-163
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• 2008

본 논문에서는 대형 구조물의 감시를 위한 새로운 광섬유 경사계를 제시한다. 이 경사계 시스템은 센서 헤드, 광 조절부 그리고 신호처리부로 이루어진다. 또한 본 시스템은 무아레 프린지 현상의 원리를 이용하며 광섬유라는 새로운 광측정 장치를 적용하였다. 이를 통해, 센서 시스템은 가격이 저렴하고 전자기학 잡음에 강하며 설치가 용이해 졌다. 실제로 본 논문에서는 저비용의 광전자 구동 시스템을 포함한 실제 시제품을 성공적으로 개발하였다. 그리고 실제 실험을 통해 센서의 선형성을 포함한 성능을 검증하였고 이를 통해 실제 토목구조물의 처짐각을 측정할 수 있는 광섬유 경사계 시스템으로서의 가능성과 성능을 입증하였다.

• 한국어병학회지
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• 제21권3호
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• pp.189-199
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• 2008

This paper aims to compare difference with the in an ability of their resistance and survival against in a non-specific defence mechanism of the olive flounder, between the virulent and the avirulent E. tarda strains. The tested E. tarda strains, we divided into the virulent and the avirulent strain groups on the basis of a value of 50% lethal dose (LD50) for the olive flounder weighed 10.3 g in average. The strains of LD50 101.6~104.2 cfu/fish were grouped as virulent strains, such as KE-1, KE-3, KE-5 and FSW910410. The group of avirulent strains as LD50 exceeded 108.7 cfu/fish were included the strains, SU100 and AL92448. A test was conducted to understand the survival ability of each strain in the mucus of the skin and the intestine of olive flounders. The results showed KE-1, KE-3, KE-5 and FSW910410 were highly to survive between 6 hours and 24 hours in intestine. The survival ability in the bile of olive flounder the number of avirulent strains declined during incubation but the virulent strain showed the number of alive bacteria having sustained or increased. In the test for the survival of bacteria in fresh sera of olive flounder, the virulent strains also had tendency to multiply. Concerning the tested bacteria internalization into the head kidney macrophages and the intracellurar replication in the macrophages of olive flounder. The virulent strains exhibited strong internalization, followed high rate replication. According to the results, virulent strains of E. tarda revealed more ability to resist and survive in the face of humoral and cellular defence factors than avirulent strains.