Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Responses of Edwardsiella tarda to innate immunity of olive flounder, Paralichthys olivaceus

넙치, Paralichthys olivaceus의 비특이적 방어기작에 대한 Edwardsiella tarda 균의 반응

  • Ha, Su-Jin (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Woo, Sung-Ho (Institute of Fisheries Science, Pukyong National University) ;
  • Lee, Deok-Chan (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
  • 하수진 (부경대학교 수산생명의학과) ;
  • 우승호 (부경대학교 수산과학연구소) ;
  • 이덕찬 (국립수산과학원 병리연구과) ;
  • 박수일 (부경대학교 수산생명의학과)
  • Published : 20081200
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Abstract

This paper aims to compare difference with the in an ability of their resistance and survival against in a non-specific defence mechanism of the olive flounder, between the virulent and the avirulent E. tarda strains. The tested E. tarda strains, we divided into the virulent and the avirulent strain groups on the basis of a value of 50% lethal dose (LD50) for the olive flounder weighed 10.3 g in average. The strains of LD50 101.6~104.2 cfu/fish were grouped as virulent strains, such as KE-1, KE-3, KE-5 and FSW910410. The group of avirulent strains as LD50 exceeded 108.7 cfu/fish were included the strains, SU100 and AL92448. A test was conducted to understand the survival ability of each strain in the mucus of the skin and the intestine of olive flounders. The results showed KE-1, KE-3, KE-5 and FSW910410 were highly to survive between 6 hours and 24 hours in intestine. The survival ability in the bile of olive flounder the number of avirulent strains declined during incubation but the virulent strain showed the number of alive bacteria having sustained or increased. In the test for the survival of bacteria in fresh sera of olive flounder, the virulent strains also had tendency to multiply. Concerning the tested bacteria internalization into the head kidney macrophages and the intracellurar replication in the macrophages of olive flounder. The virulent strains exhibited strong internalization, followed high rate replication. According to the results, virulent strains of E. tarda revealed more ability to resist and survive in the face of humoral and cellular defence factors than avirulent strains.

Keywords

References

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