• 제목/요약/키워드: Immune reaction

검색결과 562건 처리시간 0.032초

다용량 비타민 C 투여가 생쥐 세포매개면역반응에 미치는 영향 (The Effects of High-dose Vitamin C Administration on the Cell-mediated Immune Response in Mice)

  • 노가화;김헌곤;신영아;임현자;문성규;이용택;이왕재;이동섭;황영일
    • IMMUNE NETWORK
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    • 제3권3호
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    • pp.211-218
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    • 2003
  • Background: Vitamin C is an essential nutrient, taken as a daily supplement by many people. Recently, high-dose vitamin C is considered as a therapeutic regimen in some clinical situations. Until now, few studies have been done with the effects of high-dose vitamin C on the immune response. Methods: In this experiment, the effects of high-dose vitamin C on cell-mediated immune response in immunologically competent mice were evaluated. After intraperitoneal injection of 2.5, 5, or 10 mg/day of vitamin C for 10 days, delayed type hypersensitivity (DTH) was provoked against DNFB in the pinnae as a model for cell-mediated immune response. Severity of DTH reaction was evaluated as the thickness of pinnae, and the vitamin C levels were measured in the serum, liver, kidney, lung, pinnae, and splenocytes. Results: After challenge, the thickness increased at its peak on the $2^{nd}$ day in all groups. On the first day, the pinnae were thicker in the injected groups than in the control. On the contrary, the increment of the pinnae thickness was attenuated and the number of cells infiltrated in the site of DTH decreased proportionately to the amount of vitamin C administered from the second day on. With vitamin C exogenously given, the serum level peaked at 30 min after injection, and returned abruptly to its basal level without accumulation. However, it accumulated in the liver, kidney, and especially in the pinnae inflamed and splenopcytes, proportionately to the amount administered. Conclusion: Based on these results, it is suggested that, in one hand, exogenously administered high-dose vitamin C accumulated in the splenocytes and presumably changed the function of them resulting in the augmented cell-mediated immune response, as was revealed in the first day of DTH reaction. On the other hand, it seems likely that the vitamin C also showed anti-inflammatory effects.

상용 한약재의 부작용에 관한 연구 (A Study on the Side Effect of Crude Drugs)

  • 안덕균;김찬수
    • 생약학회지
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    • 제14권3호
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    • pp.102-106
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    • 1983
  • As many crude drugs are used in the oriental medical field problems on the side effects of these drugs come to the front. To conduct delayed-type hypersensitivity we selected 29 kinds of drugs used frequently for therapeutic agents in oriental medical hospitals (Table I). The cell-mediated immune response was evaluated by measuring the foot pad swelling reaction and humoral immune response by measuring the antibody formation to these crude drugs. Mice were given these drugs intraperioneally for sensitization and challenged with same drug as used for sensitization respectively by intral dermal injection on the left and righ hind foot pad 4 days after senstization and then the foot pads were measured with the dial micrometer. The results were as follow; 1) Gentianae Scabrae Radix, Arecae Semen, Corydalis Tuber, and Paeoniae Radix were significant as delayed-type hypersensitivity inducers. 2) None of the crude drugs tested had effect on the induction of humoral immune response.

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Ethanol의 면역독성에 대한 인삼엑기스의 영향 (Effect of Panax ginseng Extracts on the Immunotoxicity of Ethanol)

  • 안영근;김정훈;이병준
    • Environmental Analysis Health and Toxicology
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    • 제3권3_4호
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    • pp.29-37
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    • 1988
  • Experiments were performed on mice to investigate the effect of panax ginseng extracts on the immunotoxicity of ethanol. Immune response were evaluated by antibody production, Arthus reaction, delayed type hypersensitivity (DTH), Rosette froming cell (RFC) and macrophage activity in mice, sensitized and challenged with sheep red blood cells. The weight of liver, spleen and thymus were measured. Following results obtained in this experiment. The exposure of ethanol decreased humoral and cellular immune response, the body weight and macrophage activity. Ginseng extracts such as ethanol extract, petroleum ether extract and n-butanol fraction were significantly increased the body weight. The administration of ginseng ethanol extract and ginseng petroleum ether extract were restored or increased humoral and cellular immune response. Macrophage activity was decreased by ethanol, but restored by the ginseng extracts.

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수종 한약재가 면역 반응에 미치는 영향 (Effects of Several Herbs on the Immune Responses)

  • 송봉근
    • 대한한의학회지
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    • 제18권2호
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    • pp.43-57
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    • 1997
  • It was claimed that the herbal medicine with the function of strengthening the body resistance exerts to enhance the immunity. And the medicine with the effect of eliminating the pathogenic factor is stated to inhibit the immune response. To evaluate the the effects of the herbal medicine on the immune response, the mice were administrated with the herbal medicine for 2 weeks. And the responses were analyzed. As the result, water extract of Radix Astragali, Fructus Psoraleae, Cortex Acanthopanacis, Semen Coicis, Herba Ecliptae, Spica Prunellae, and Radix Sophorae increased the ROI production, while Radix Tripterygia inhibited it. Phagocytic activity was increased after administration of Radix Astragal, Fructus Psoraleae, Cortex Acanthopanacis, Herba Ecliptae, Spica Prunellae and Radix Sophorae. NK cell activity was also significantly inhibited by Radix Tripterygia. Administration of Radix Astragali, Fructus Psoraleae, Cortex Acanthopanacis, Herba Ecliptae, Spica Prunellae and Semen Coicis enhanced the antibodies(hemagglutinin and hemolysin) formation and the appearance of rosette forming cells of the spleen, while Radix Sophorae and Radix Tripterygia decreased it. Radix Sophorae and Radix Tripterygia also decreased the allogenic immune response and mixed-lymphocyte reaction. And all the experimental herbs decreased contact hypersensitivity against dinitroflurobenzene. These results show Radix Astragali, Fructus Psoraleae, Spica Prunellae, Cortex Acanthopanacis, Semen Coicis and Herba Ecliptae enhanced innate immunity, humoral and cellular immune responses. However Radix Sophorae and Radix Tripterygia exert imunosuppressive action. Also these results indicate that the medicine with the action of the strengthening the body resistance enhances the immunity. And the the some of drugs belonging to the eliminating the pathogenic factor also increase the immune responses.

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四君子湯이 免疫機能에 미치는 影響 (Effect of Sagunja-tang on Immune Function of Mouse Immune Cells)

  • 이상현;정명;임규상;윤용갑
    • 한방안이비인후피부과학회지
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    • 제28권3호
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    • pp.14-29
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    • 2015
  • Objectives : The extract of Sagunja-tang has been traditionally used for restorative treatment of constitutional weakness, vascular and immune disorder, and nervous disease in Oriental country. This study investigated the regulatory effects of Sagunja-tang on the expression, production, and activity of immune mediators.Methods : In this study, the extract of Sagunja-tang was prepared by extracting with distilled water at 100$^{\circ}C$ for 2.5h. The extract was freeze-dried following filtration through 0.45${{\mu}m}$ filter. The extract was dissolved in Hank's balanced salt solution (HBSS) and filtered again through 0.45${{\mu}m}$ filter before use. The level of nitrite, an oxidative product of nitric oxide(NO) was measured in the culture medium by the Griess reaction. The levels of prostaglandin E2(PGE2), Th1 cytokines (IFN-${\gamma}$, IL-2) and Th2 cytokines(IL-4, IL-5, IL-13) were measured by enzyme-linked immunosorbent assay and the protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression were determined by Western blot analysis. Also examined the effects of the extract on T-cell proliferation and cytotoxic activity of natural killer cells.Results : In this investigation, Production levels of Th2 cytokines (IL-4, IL-5, IL-13) was inhibited in a dose dependent manner by treatment with the extract. I also found that the extract increased T-cell proliferation and cytotoxic activity of natural killer cells in a dose-dependent manner.Conculsions : These results suggest that the water extract of Sagunja-tang may be useful for a therapeutic drug against a sickly constitution and immune diseases, probably by regulating the production of immune mediators.

Benzo(a)pyrene의 면역독성에 미치는 인삼의 영향 (The Effect of Ginseng on the Immunotoxicity of Benzo(a)pyrene)

  • 안영근;김주영;신하경;정종갑
    • Environmental Analysis Health and Toxicology
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    • 제1권1호
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    • pp.47-54
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    • 1986
  • The immunopotentiating effect of ethanol extract, butanol fraction and petroleum ether extract of Panax ginseng on the immunotoxicity of benzo(a)pyrene were investigated in mice. A single administration of benzo(a)pyrene induced an apparent but relatively transient reduction in HY titer, Arthus reaction, delayed type hypersensitivity, rosette forming cell and natural killer cell activity Ethanol extract very significantly restored HY titer, Arthus reaction. RFC and natural killer cell activity. Butanol fraction have no effect. But petroleum ether extract very significantly restored humoral and cellular immune response and especially natural killer cell activity.

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구름버섯의 항암성 다당류분획(Copolang)이 마우스의 면역기능에 미치는 영향에 관한 연구 (Antitumor Activity of the Polysaccharide-Fraction(Copolang) from Coriolus versicolor and its Effects on the Immune Function)

  • 문창규;이수환;목명수;김대욱
    • 약학회지
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    • 제31권2호
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    • pp.126-132
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    • 1987
  • Polysaccharide fraction isolated from Coriolus versicolor (Copolang) was studied on the antitumor activity and immunostimulation activities with reference to PS-K. Copolang showed nearly equal antitumor activities to the PS-K and exhibited marked augmentation effects on the antibody mediated hypersensitivity reaction, delayed type hypersensitivity reaction and NK-cell activity in tumor bearing mice. But it did not show any noticeable effect on the antibody secreting cell and macrophage function in normal mice. These results indicate that the antitumor activity and immunostimulating effect of Copolang are comparable to those of PS-K.

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Acriflavine 및 중금속이온반응에 의한 Brucella R형균 검출에 관한 연구 (STUDIES ON THE AGGLUTINATION REACTION OF BRUCELLA R-FORM BY ACRIFLAVIN AND HEAVY METAL IONS)

  • 강병규
    • 대한수의사회지
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    • 제10권1호
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    • pp.13-23
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    • 1966
  • 1. To evaluate the quantitative relationship between S-R variation in 8r. abortus strain 19 and Br. melitensis 16 M induced by homologous immune serum treatment was studied by means of tube agglutination reaction with acriflavine add various heavy metal i

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마우스에 있어서 에이코사펜타엔산이 면역반응(免疫反應)에 미치는 영향(影響)( I ) -I. 체액성(體液性) 면역(免疫)- (The Effect of Eicosapentaenoic Acid on the Immune Response in Mice(I) -I. Humoral-mediated immunity-)

  • 안영근;김정훈;이상근;김행순
    • 약학회지
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    • 제33권1호
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    • pp.20-29
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    • 1989
  • The humoral immune response of Eicosapentanoic acid(EPA) was investigated in mice. ICR male mice were divided into 8 groups and received intraperitoneal injection of EPA(5 mg, 10 mg, 20 mg/kg) for 4 weeks. Cyclophosphamide(5 mg/kg) was administered i.p. 2 days prior to secondary immunization. Humoral immune response was evaluated by antibody titer, hypersensitivity to SRBC (Arthus), plaque forming cell(PFC) and organ weight. The ontanined results were as followings: The increased rate of body weight, the ratio of liver weight, spleen weight to body weight were decreased by all EPA administration groups as compared to normal group. HA titer, HY titer and Arthus reaction were enhanced according to the increase of EPA doses as compared to normal group. PFC was significantly enhanced by EPA 10 mg administration group. These results suggest that EPA enhances humoral immune response to SRBC in mice, indicating that EPA may block a immunoglobulin synthesis inhibition of arachidonic acid.

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Kelp (Ascophyllum nodosum) meul 첨가 사료가 돌돔 (Oplegnathus fasciatus)의 성장과 비특이적 면역 반응에 미치는 영향 (Effects of Kelp (Ascophyllum nodosum) Meal on Growth and Nonspecific Immune Responses of Parrot Fish (Oplegnathus fasciatus))

  • 원경미;김병기;박수일;유병서
    • 한국수산과학회지
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    • 제37권4호
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    • pp.275-280
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    • 2004
  • In this study, the effcts of kelp (Ascophyllum nodosum) meal on growth and immune responses of parrot fish (Oplegnathus fasciatus) were studied. Fish were fed an experimental diet supplemented with $2{\%}\;and\;5{\%}$ kelp meal in a controled diet. Several factors such as weight gain, hematological parameters and nonspecific immune responses were evaluated far 0, 2, 4 and 8 weeks after the administration of the kelp meal supplemented diet. Weight gain in the fish fed the diet supplemented with $2{\%}\;and\;5{\%}$ kelp meal was not significant among the tested groups. The NBT reaction of the phagocytes in the head kidney and the phagocytic rate/index of phagocytes in the peripheral blood were significantly higher than the control group. But, there was no significant difference both in complement bactericidal activity, mucus Iysozyme activity and hematology among each group.