• 제목/요약/키워드: Immobilized cell

검색결과 320건 처리시간 0.025초

고정화된 효모로 충전된 관형발효기에서의 에탄올 연속발효 (Comparative Study on Continuous Ethanol Fermentation by Immobilized Tubular Fermentor)

  • 서근학;최명호;송승구
    • 한국미생물·생명공학회지
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    • 제16권3호
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    • pp.205-212
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    • 1988
  • Saccharomyces formosensis를 wood chip과 alginate gel에 고정화 실험을 한 결과, 모두 효모 고 정화도 매우 높으므로 고정화를 인한 Support로서 적절한 것으로 판단되었다. 관형발효기에 효모를 고 정화한 wood chip 및 alginate gel를 충전하여 연속발효 실험을 수행한 경우 0.446-0.485g EtOH/ g glucose로서 비슷하였으나, cell 수율은 alginate gel의 경우가 wood chip의 경우보다 낮아서 down stream의 처리시 유리하였다. 관형발효기의 에탄을 생산성은 wood chip을 이용할 경우 정상상태에서 에탄을 농도 68.3-54.9g/$\ell$ 범위에서 17.1-32.6g EtOH/$\ell$.hr를 나타내었고, alginate gel을 이용할 경우 정상상태에서 에탄올 농도 80.0-56.8g/$\ell$범위에서 에탄올 생산성은 20.0-32.0g EtOH/ hr를 얻었다. 본 실험의 에탄올 생산성은 다른 고정화법에 비하여 높았으며, 고정화 방법 중 alginate gel을 이용한 고정화법은 에탄을 생산을 위하여 효과적인 에탄을 생산 방법으로 사료되었다.

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Effects of high Cell Density on growth-Associated Monoclonal Antibody Production by Hybridoma T0405 Cells Immobilized in Macroporous Cellulose carriers

  • Hideki Mochoda;Wang, Pi-Chao;Fr Jr. Nayve;Ryuji Sato;Minoru Harige;Nakao Nomura;Masatoshi Matsumura
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제5권2호
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    • pp.110-117
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    • 2000
  • Relationship between monoclonal antibody (MAb) productivity and growth rate, and effects of high cell density on MAb production rate increased with increasing specifis growth rate in both suspended and immobilized continuous cultures indicate a positively growth-associated relationship between MAb productivity and growth rate. moreover, the specific production rate was higher in the immobilized cell culture than that in suspended one at all dilution rates. In order to clarify these phenomana, MAb mPNA experession and cell cycle distribution were investigated in bacth cultures with immobilized cells and suspended cells. RT-PCR was used for observation of MAb mRNA expression and a two-color bromodeoxyuridine (BrdU)/propidium iodide (PI) flow cytometry method for determination of cell cycle distribution. The results revealed that MAb nRNA expression until dead phase, which was longer than in suspended cell. The cell cycle distribution patterns were observed almost the same for both immobilized and suspended cells. Such results may imply that a high cell density state has positive influence on the mRNA expression and on growth-associated Mab productivity of T0405 cells.

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Improved mevinolic acid (MA) production by the immobilized cells, and the establishment of on-line measurement system for fermentation parameters using vent gas analyzer

  • 송성기;김경희;김명진;이상종;장용근;정연호;정용섭;전계택
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2003년도 생물공학의 동향(XII)
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    • pp.223-227
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    • 2003
  • Mevinolic acid (MA), a secondary metabolite produced by a filamentous fungus Aspergillus terreus, is acidic form of lovastatin which has been identified as a powerful cholesterol-lowering agent in humans. When immobilized cell culture was performed, MA production was about 5.3-fold higher than the parallel suspended cell culture. Although the immobilized cells proliferated slowly during exponential in comparison with the suspended cells, oxygen uptake rate and oxygen mass transfer coefficient of the immobilized cell culture were about 1.3- and 2.5- fold higher respectively than those of the parallel suspended cell culture. From these results, it was concluded that MA biosynthesis was closely dependent on the cell growth rate, morphology and oxygen availability.

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동물세포 배양액으로부터 암모늄 이온의 동시제거를 위한 고정화 흡착제의 개발과 동물세포 배양 시스템에의 응용: I. 고정화 흡착시스템 개발 (Development of an Immobilized Adsorbent for in situ Removal of Ammonium Ion from Mammalian Cell Culture Media and its Application to a Mammalian Cell Bioreactor: I. Development of Immobilized Adsorbent System)

  • 박병곤;민용원;전계택;김익환;정연호
    • KSBB Journal
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    • 제13권4호
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    • pp.404-410
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    • 1998
  • CHO 세포외 성장 및 생산성을 저해하는 엄포늄 이온의 동시 제거를 위해 PhJlhpsite~Gismondine 합성 zeolite가 alginate, cellulose acetate. dialysis membrane에 고정화된 흡착제가 개발되었다. 고정화 흡착체에 의한 암모늄 이온의 제거 효율 및 비에 따른 세표성장의 증진효과를 비교한 결과 최적의 고정화 흡착제로 membrane type이 선정되었다. 암모늄 이온의 제거 효파를 더욱더 명확하게 하고 고멸도의 세포 배양을 모사하가 위하여 8mM의 ammonium chloride를 첨가하고 membrane type 고정화 흡착제를 투여하여 암모늄 이온의 동시제거 효과를 조사한 결과 최대 세포 농도가 3배 이상 증가하였으며 생존율 역시 증가하였고 40%정도의 tPA 생산성 향상올 보여 주었다­. 이러한 증진 효과는 암모늄 이온의 고농도 시스템일수록 커졌다. 고정화 흡착제의 최적 투며 시기를 조사해본 결과 ammonium chlonde를 첨가하지 않았을 때 membrane type 고 정화 흡착제의 최적 투여 시기는 배양 시작 후 48시간이었고, 8mM의 ammonium chloride를 침가한 경우의 최적 투여 시기는 배양 시작 후 72시간이었다.

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발포성 포도주의 생산에 고정화 효모의 이용 (Using of Immobilized Yeast Cells for the Production of Sparkling Wine)

  • 이용수;이건표;최진상
    • 한국식품저장유통학회지
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    • 제5권2호
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    • pp.186-190
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    • 1998
  • In order to investigate the possible application of immobilized yeast cells in sparkling wine production instead of riddling puns by the traditional method, fermentation characteristics were tested during the sparkling wine fermentation in the bottle using immobilized yeast cells with alginate. The rates of sugar consumption and alcohol production were faster with free cells than those with immobilized cells during the fermentation. The higher concentration of yeast cells and the lower concentration of alginate in the cell immobilization resulted in the faster sugar consumption and alcohol production. It also resulted in the increase of yeast cell concentration released from immobilized beads during the fermentation. However, no differences were shown in the contents of alcohol, residual sugar and CO2 pressure after fermentation. In case concentration of yeast cells released from immobilized beads during bottle fermentation, the higher concentration of alginate had and the lower had.

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Stabilization of Bioluminescence of Immobilized Photobacterium phosphoreum and Monitoring of Environmental Pollutants

  • Britz, Margaret L.;Nina Simonov;Chun, Uck-Han
    • Journal of Microbiology and Biotechnology
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    • 제7권4호
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    • pp.242-249
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    • 1997
  • Stability of bioluminescence was investigated with Photobacterium phosphoreum immobilized on the strontium alginate in order to develope continuous real time monitoring of pollutants. The stability of bioluminescence emission was improved by prolonged aging time. The aging time of ${\geq}40$ min and the cell concentration of ${\leq}0.6\;of\;OD_660$ were selected for the immobilization of P. phosphoreum to give linearity between cell concentrations and bioluminescence intensity. In sensitivity tests using phenol, it was found that this compound quenched bioluminescence proportional to the concentration without lowering of cell growth. The lower value for maximum quenching ($q_s$) and higher dissociation constant ($K_s$) were observed with strontium-alginate immobilized cells compared to free cells. The response of bioluminescence to toxicants was evaluated with the immobilized luminescent bacteria. The sensitivity of the immobilized cells was found to be good in response to toxicants, 4-nitrophenol, salicylate and cadmium, when evaluated with a specific rate of bioluminescence quenching.

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고정화된 Chromobacterium chocolatum의 Whole Cell을 이용한 Dimethyl-cis- 1,3-dibenzyl-2-oxoimidazolidine-4,5-dicarboxylate의 가수분해 (The Hydrolysis of Dimethyl-cis-1,3-dibenzyl-2-oxoimidazolidine-4,5-dicarboxylate by Immobilized Whole Cells of Chromobacterium chocolatum)

  • 이윤진;심상균;안용현
    • 대한화학회지
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    • 제41권9호
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    • pp.483-487
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    • 1997
  • Chromobacterium chocolatum의 whole cell을 polyacrylamide의 matrix안에 고정화 하였으며 이를 이용하여 dimethyl-cis-1,3-dibenzyl-2-oxoimidazolidine-4,5-dicarboxlyate를 가수분해 하였다. 고정화된 mycelium을 이용하여 가수분해하는 최적조건을 연구하였다. 기질의 농도, 반응시간, 용액의 pH변화에 다른 lipase의 안정성및 반응의 영향을 연구하였다. 고정회된 mycelium의 리파아제의 활동도는 4주이상 지속되었고 wet cell 2g을 고정화 하여 200 mg의 기질을 pH=7의 반응조건에서 반응시켰을 때 최대 수율로 생성물이 얻어졌다.

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A study on the whole cell immobilized glucose oxidase from Aspergillus niger

  • Choe, I.S.;Roh, J.K.;Han, M.H.
    • 한국미생물생명공학회:학술대회논문집
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    • 한국미생물생명공학회 1979년도 추계학술대회 심포지움
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    • pp.245.2-246
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    • 1979
  • Heat treated whole cell of Aspergillus niger containing glucose oxidase-catalase system was entrapped in gelatin matrix crosslinked by glutaral-dehyde. The reaction characteristics of immobilized enzyme was studied in a fludized reactor. Heat treatment enhanced the stability and improved the properties of micellium for the immobilized process. The immobilized enzyme system showed the maximum activity at $35^{\circ}C$ and at pH 5.5. The optimum substrate concentration was 0.04M glucose. The activity of immobilized glucose oxidase was in proportion to the concentration of dissolved oxygen in reaction mixture as other reaction conditions were fixed. It was also demonstrated that the limiting factor for the activity of the immobilized glucose oxidase was the oxygen diffusion resistance which increases proportionally to the glucose concentration.

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Repeated-batch Culture of Immobilized Gibberella fujikuroi B9 for Gibberellic Acid Production: An Optimization Study

  • Kim, Chang-Joon;Lee, Sang-Jong;Chang, Yong-Keun;Chun, Gie-Taek;Jeong, Yeon-Ho;Kim, Sung-Bae
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제11권6호
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    • pp.544-549
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    • 2006
  • The performance of immobilized fungal cells on celite beads for the production of gibberrelic acid was investigated in flasks and 7-L stirred-tank reactor. Repeated incubations of immobilized fungal cells increased cell concentrations and volumetric productivity. The maximum volumetric productivity obtained in the immobilized-cell culture was 3-fold greater than that in suspended-cell culture. The concentration of cotton seed flour (CSF), among the various nutrients supplied, most significantly influenced productivity and operational stability. Notably, insoluble components in CSF were found to be essential for production. CSF at 6 g/L with 60 g/L glucose was found to be optimal for gibberellic acid production and stable operation by preventing excessive cell growth.

Kasugamycin 생산을 위한 연속 청정생물공정 개발 (Development of Continuous Clean Bioprocess for Kasugamycin Production)

  • 김창준;박선옥;장용근;전계택;이종대;김상용
    • 청정기술
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    • 제4권1호
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    • pp.45-59
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    • 1998
  • 방선균인 Streptomyces kasugaensis에 의해서 생산되는 이차대사산물인 kasugamycin 생산성 증대 및 단위 생산량 당 오염물질 배출량 저감을 위한 연속 고정화 배양을 수행하였다. 세포 고정화에 적합한 포자 수확을 위한 포자형성 촉진 배지 개발은 물론 고정화 담체로 사용한 셀라이트에 수확된 포자를 고정화시키는 방법을 확립하였다. 연속 고정화 배양 시 고정화세포의 유출을 방지함으로써 안정된 연속배양을 가능토록 하기위한 decantor 형태의 고정화세포 분리기가 장착된 반응기를 사용하였다. 희석 속도 및 공급액 중의 기질 농도(당 농도, 인 농도)를 변화시키며 생합성된 kasugamycin 생산성 및 화학적산소요구량(COD)을 측정하였으며 이들을 현탁세포를 이용한 회분식 발효에서의 kasugamycin 생산성 및 발효 폐액 중의 COD 값과 비교하였다. 고정화 세포를 이용한 연속배양에서의 kasugamycin 생산성이 현탁세포를 이용한 회분식 발효에 비해 2.5배 높았으며, 동시에 단위 생산량 당 COD가 2.3배 저감되는 것으로 나타났다. 이것으로부터 연속 고정화 배양이 현탁 회분식 배양에 비해 kasugamycin 생산성 및 오염물질 배출 측면에서 유리한 청정 공정임을 확인할 수 있었다.

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