• Preventive Nutrition and Food Science
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• 제21권4호
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• pp.323-329
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• 2016

Achyranthes japonica Nakai (AJN) water extract has a variety of physiological properties, including anti-diabetic, anti-cancer, anti-inflammatory, anti-microbial, and anti-oxidative activities. In the present study, the inhibitory effects of AJN extract were investigated in high affinity immunoglobulin E receptor ($Fc{\varepsilon}RI$)-mediated KU812F cells activation. AJN extract showed suppressive effects on histamine release and intracellular calcium [$Ca^{2+}$]i elevation from anti$Fc{\varepsilon}RI$ antibody (CRA-1)-stimulated cells in a dose-dependent manner. Flow cytometric analysis showed that AJN extract treatment caused a dose-dependent decrease in the cell surface $Fc{\varepsilon}RI$ expression and the binding between the cell surface $Fc{\varepsilon}RI$ and the IgE antibody. Moreover, reverse transcription-polymerase chain reaction analysis showed that levels of the mRNA for the $Fc{\varepsilon}RI$ ${\alpha}$ chain was decreased by treatment with AJN extract. These results indicate that AJN extract may exert anti-allergic effects via the inhibition of calcium influx and histamine release, which occurs as a result from the downregulation of the binding of IgE antibody to cell surface $Fc{\varepsilon}RI$. This mechanism may occur through $Fc{\varepsilon}RI$ expression inhibition.

• Parasites, Hosts and Diseases
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• 제34권4호
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• pp.255-258
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• 1996

닭에 있어서 닭와포자충의 재감염에 대한 방어기전을 이해하기 위하여 장기간에 걸쳐 혈청IgG항체가를 경시적으로 조사하였다. 2일령의 SPF 병아리(Dekalb-Warren, Sex-Sal-Link)에 $5{\;}{\times}{\;}10^5$개 그리고 IgG가가 정상으로 복귀한 다음 10일인 340일령에 $1{\;}{\times}{\;}10^7$개의 닭와포자충의 오오시스트를 경구투여하고 나서 채혈하여 간점형광항체법을 이용하여 혈청IgG 항체가를 측정하였다. IgG 가는 감염후 3일부터 38일까지는 대조군과 차이가 없었으나 45일부터 1:21.33으로 상승하기 시작하여 120일에 1:512로 최고치에 이른다음 점점 떨어져 330일에 1:4로 정상치에 이르렀다. 도전 감염후 7, 14, 28일에 각각 1:16, 1:512, 1:1.024로서 점점 높아져 정점에 이른다음 급격히 떨어져 63일에 정상으로 복귀하였다. 한편, 대조군의 항체가는 모두 1:2~1:4의 범위에 있었으며 이 원충의 도전감염전 및 후에 있어서 분변으로부터 집충시켜 제작한 Kinyoun 항산염색표본에서도 오오시스트를 전혀 검출할 수 없었다. 분변내의 오오시스트의 소실 시기와 최초 감염에 있어서 IgG가의 피이크는 일치하지 않았다.

• Parasites, Hosts and Diseases
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• 제29권4호
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• pp.339-354
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• 1991

스파르가눔을 흰쥐와 흰생쥐에게 인긍 구강감염을 시켜 감염 2주후부터 숙주의 기생부위에서 충체와 함께 둘레의 조직을 적출하여 동결절편을 만들고 이것을 ABC면역효소표지법과 간접형광항체법 (IFA)으로 항원성분의 분포와 그 추이를 추구한 결과, IgG 항체에 의한 면역반응이 충체의 망상유조직에서 인지되었으며 특히 ABC면역효소표지법에 의하면 유조직 중 수질에서 보다 피질층에서 강한 반응성을 나타났다. 이 중에서도 석회소체가 있는 곳에 염색 반응성이 강하였다. 감염후기에서는 스파르가눔의 외피 (tegument) 내외면과 충체를 둘러 싸고 있는 피낭의 막면, 피막 밖에 있는 섬유성 결합조직과 근조직의 간극에서도 항원성분을 관찰할 수 있다. 특히 결합조직 사이에 분포된 소혈관의 둘레와 현관 외벽에 강한 반응이 나타났다. IgM 항체에 인지된 항원성분도 충체의 유조직층중 피질충에서 강한 반응이 나타났다. 스파르가눔의 추출 조항원성분과 배설분비 항원성분을 SDS-PAGE한 후 nitrocellulose 막에 영동전이하여 IgG, IgM 항체를 유도하는 항원성분을 ABC효소표지 항체 법으로 조사하였다. 스파르가눔의 추출 조항원 성분 중 흰쥐의 IgG 항체에 인지된 항원 성분은 23개이고 IsM 항체에는 15개의 항원성분이 인지되었다. 흰생쥐에서는 IgG 항체로 I6개 항원성분이, IgM 항체에 9개의 항원성분이 인지되었다. 배설분비 항원성분 중 횐쥐의 IgG 항체에 20개 항원성분이, IgM 항체에는 5개의 항원 성분이 인지되었다. 횐생쥐의 IgG 항체에 18개, IgM 항체에 11개의 항원성분이 인지되었다. 조창원성분 중 15개 항원성분과 배설분비 항원성분중 13개의 항원 성분이 흰쥐, 흰생쥐의 IgG 항체에 모두 교차반응이 일어났으며 IgM 항체에 반응한 항원성분중 IgG 항체와도 교차반응이 있었다.

• 한국가금학회지
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• 제22권2호
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• pp.85-95
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• 1995

This study was conducted to investigate the immune response of layers fed diets supplemented with excess micronutrients, i.e., vitamin A, methionine, Zn, Cu, and Fe to the inoculation of Newcastle disease vaccine(NDV) or infectious bronchitis vaccine(IBV). The antibody titer against the NDV increased immediately after the inoculation and stayed high during the next 6 wk. On the other hand, The antibody titer against the IBV increased after 4 wk of inoculation The IgM level increased rapidly after 1 wk of NDV inoculation, however, it decreased after 5 wk of inoculation. The IgA displayed similar pattern to that of IgM in response to NDV inoculation. The pattern of IgM change after IBV inoculation was similar to that when layers were treated with NDV. However, IgA level changed earlier than did IgM. The IgG response to the NDV and IBV was very weak compared to the other immune responses. The excess supplementation of micronutrients to the diets of layers inoculated with NDV elicited favorable antibody titer and immune response compared to the layers fed the control diet. The excess Zn, however, allowed the layers to have higher antibody titer for the 4-wk period after NDV injection: after that they showed no effect of extra-Zn. The immune responses of layers fed excess vitamin A, Cu, methionine, and Fe were markedly higher in IgA and IgG than the control layers. The excess Zn, however, did not bring about any favorable result. No difference was detected in IgG level between control and micronutrients-treated groups.

• 생명과학회지
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• 제17권11호
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• pp.1492-1496
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• 2007

CD40L를 발현하는 KU812세포와 CD40를 발현하는 항원 특이적 B 세포의 배양을 통해 IgE 클라스 스위치가 유도된다. 본 연구자는 체외면역법에 의해 건강인의 말초혈액을 이용하여 쌀 알레르겐 특이적 IgM 항체를 생성하는 B 세포주를 수립하였다. 본 연구에서는 KU812세포에 의한 쌀 알레르겐 특이적 IgE 항체의 유도를 시도하였다. 배양 전, 쌀 알레르겐 특이적 B 세포주, RA9G11과 PMA와 ionomycin을 6시간 처리하여 활성화된 KU812세포에서 CD40와 CD40L 발현량을 flow cytomerty를 통화여 각각 확인하였다. RA9G11세포와 활성화된 KU812세포는 높은 수준의 CD40와 CD40L를 각각 발현하였다. 쌀 알레르겐 특이적 IgE항체 합성 유도를 위해, 여러 가지 농도의 IL-4 존재하에, RA9G11세포와 활성화된 KU812세포를 12일 동안 배양하였다. IgE 정상영역의 mRNA 발현량과, 쌀 알레르겐 특이적 IgE 항체 생성 세포가 모든 조건에서 확인되었으며, 특히 50U의 L-4 농도 하에서 같은 비율의 RA9G11과 활성화된 KU812세포를 배양했을 때, IgE 합성이 가장 높았다. 따라서 활성화된 KU812세포에 의한 쌀 알레르겐 특이적 IgE 합성은 알레르기 질환의 치료 및 예방에 관한 연구에 기여할 것으로 사료된다.

• 한방안이비인후피부과학회지
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• 제13권2호
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• pp.165-181
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• 2000

Sinichengpaeum has been widely used in the oriental medical treatment of nasal diseases. These studies were performed done to investigate the effect of Sinicheng- paeum on the anti-allergic action We studied the vascular permeability response induced by the histamine and serotonin injection(allergy Ⅰ type), homologous PCA provoked by the IgE-like antibody against EWA(allergy Ⅳ type), contact dermatitis induced by picryl chloride(allergy Ⅳ type), delayed type hypersensitivity response to SRBC (allergy Ⅳ type), and the amount of eosinophil and IgE. The results were as follows: 1. The effect of Sinichengpaeum on vascular permeability responses to intradermal histamine and serotonin were significant. 2. In the homologous PCA provoked by the IgE-like antibody against white egg albumin, Sinichengpaeum showed a significant effect. 3. In the delayed type hypersensitivity responses to picryl chloride, Sini-chengpaeum provoked a significant effect. 4. After reaction provoked by picryl chloride, Sinichengpaeum showed an insignficant effect on amount of eosinophil, but a significant effect in IgE amount. 5. In the delayed type hypersensitivity responses to SRBC, Sinichengpaeum provoked a significant effect. 6. After reaction provoked by SRBC, Sinichengpaeum showed a significant effect on amount of cosinophil, but an insignificant effect in IgE amount.

• Nuclear Medicine and Molecular Imaging
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• 제40권4호
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• pp.211-217
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• 2006

재조합된 scFv lym-1의 세포결합 실험에서 높은 면역반응성을 보였으며, 비특이 반응에서 모항체인 IgG lym-1에 의해 결합이 억제됨을 확인하였고, 동물 영상을 통해 IgG보다 분자랑이 작은 scFv lym-1 항체가 빠른 체내대사율과 종양섭취율을 보여 방사면역진단에 유용할 것으로 보여진다.

• Parasites, Hosts and Diseases
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• 제28권2호
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• pp.91-100
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• 1990

폐흡충(Paragonimus Tuestermani) 피낭유충을 흰쥐(Wistar) 및 고양이에 감염시키고 감염 힐청이나 분회분리된 IgG또는 보체가 정상 또는 감염 흰쥐 복강 대식세포의 폐흡충 유충 살충에 어떠한 영향을 미치는지 부착 실험 (adherence assay) 및 세포독성을 통하여 관찰하였다. 폐흡충 감염은 복강 대식세포를 비특이적으로 활성화시퍼 대식세포의 유충에 대한 부착률 및 세포독성을 증가시켰으며, 감염 혈청을 첨가하였을 때 항체-의존 세포매개성 세포독성에 의해 배양 6시간 후에 세포 부착률 및 세포독성이 가장 강하였다. 감염 혈청을 56℃에서 30분간 가열하였을 때 IgG 항체 변성에 의해 세포독성이 저하되었다. IgG 및 보체를 첨가한 경우 세포 부착률은 낮았으나 24시간 후에는 유충이 사멸하였다. 그러나 보체의 단독적인 역할은 이 실험에서 알 수 없었다.

• 대한미생물학회지
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• 제17권1호
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• pp.75-85
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• 1982

In order to demonstrate the effect of specific serum IgG antibody on the adherence of Streptococcus mutans to smooth surface and the mechanism of effective adherence inhibition by IgG antibody, in the present study authors obtained purified IgG from different immunogen preparations of S. mutans NCTC 10449(serotype c) and observed the effect of each IgG preparation on the adherence of each S. mutans strain cultured in different conditions. In addition, the present study was undertaken to observe the cross-reactivity of IgG and the effect of sucrose concentration on the adherence of S. mutans in vitro non-growth condition. The adherence of S. mutans to glass surface was effectively inhibited by serum IgG antibody. At the same IgG concentrations, anti-2% fructose grown/1N NaCl washed S. mutans NCTC 10449 cell showed greater adherence inhibitory effect to S. mutans strains than anti-2% sucrose grown and anti-S. mutans NCTC 10449 cell wall, and the greater inhibitory effects of IgG preparations were observed in assay using 2% fructose grown S. mutans cell preparations than using 0.1% sucrose grown cell preparations. These results suggest that the more effective adherence inhibition by serum IgG antibody is due to the reaction with S. mutans cell surface antigens rather than glucan and cell-associated glucosyltransferase. The greatest adherence inhibitory effect of IgG to S. mutans strains was observed on homologous NCTC 10449 strain and the inhibition cross-reactivities were observed between serotype c, e, and f strains. More pronounced cross-reactivity of adherence inhibition of IgG to S. mutans was observed in assay using anti-2% fructose grown/1N NaCl washed cell than using other IgG preparations, and observed in assay using 2% fructose grown S. mutans cell preparations than 0.1% sucrose grown cell preparations. It was interested that low, but adequate concentration of reactive IgG antibody significantly increased the adherence ability of S. mutans. This result may be due to the formation of small cell aggregates resulted in a increase in the numbers of organisms which adhered to glass surface. The adherence of S. mutans to glass surface was possible in the absence of glucan-synthetic activity. Low level of sucrose significantly increased the adherence ability of S. mutans to glass surface, but excessive amount of sucrose induced large cell aggregates resulted in a decrease in the numbers of organism which adhered.

• Parasites, Hosts and Diseases
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• 제56권1호
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• pp.61-70
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• 2018

We developed a Rapid Diagnostic Test (RDT) kit for detecting IgG/IgM antibodies against Zika virus (ZIKV) using monoclonal antibodies to the envelope (E) and non-structural protein 1 (NS1) of ZIKV. These proteins were produced using baculovirus expression vector with Sf9 cells. Monoclonal antibodies J2G7 to NS1 and J5E1 to E protein were selected and conjugated with colloidal gold to produce the Zika IgG/IgM RDT kit (Zika RDT). Comparisons with ELISA, plaque reduction neutralization test (PRNT), and PCR were done to investigate the analytical sensitivity of Zika RDT, which resulted in 100% identical results. Sensitivity and specificity of Zika RDT in a field test was determined using positive and negative samples from Brazil and Korea. The diagnostic accuracy of Zika RDT was fairly high; sensitivity and specificity for IgG was 99.0 and 99.3%, respectively, while for IgM it was 96.7 and 98.7%, respectively. Cross reaction with dengue virus was evaluated using anti-Dengue Mixed Titer Performance Panel (PVD201), in which the Zika RDT showed cross-reactions with DENV in 16.7% and 5.6% in IgG and IgM, respectively. Cross reactions were not observed with West Nile, yellow fever, and hepatitis C virus infected sera. Zika RDT kit is very simple to use, rapid to assay, and very sensitive, and highly specific. Therefore, it would serve as a choice of method for point-of-care diagnosis and large scale surveys of ZIKV infection under clinical or field conditions worldwide in endemic areas.