Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Achyranthes japonica Nakai Water Extract Suppresses Binding of IgE Antibody to Cell Surface FcεRI.

  • Shim, Sun Yup (College of Pharmacy, Sunchon National University) ;
  • Lee, Mina (College of Pharmacy, Sunchon National University) ;
  • Lee, Kyung Dong (Department of Oriental Medicine Materials, Dongshin University)
  • Received : 2016.07.08
  • Accepted : 2016.09.13
  • Published : 2016.12.31
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Abstract

Achyranthes japonica Nakai (AJN) water extract has a variety of physiological properties, including anti-diabetic, anti-cancer, anti-inflammatory, anti-microbial, and anti-oxidative activities. In the present study, the inhibitory effects of AJN extract were investigated in high affinity immunoglobulin E receptor ($Fc{\varepsilon}RI$)-mediated KU812F cells activation. AJN extract showed suppressive effects on histamine release and intracellular calcium [$Ca^{2+}$]i elevation from anti$Fc{\varepsilon}RI$ antibody (CRA-1)-stimulated cells in a dose-dependent manner. Flow cytometric analysis showed that AJN extract treatment caused a dose-dependent decrease in the cell surface $Fc{\varepsilon}RI$ expression and the binding between the cell surface $Fc{\varepsilon}RI$ and the IgE antibody. Moreover, reverse transcription-polymerase chain reaction analysis showed that levels of the mRNA for the $Fc{\varepsilon}RI$ ${\alpha}$ chain was decreased by treatment with AJN extract. These results indicate that AJN extract may exert anti-allergic effects via the inhibition of calcium influx and histamine release, which occurs as a result from the downregulation of the binding of IgE antibody to cell surface $Fc{\varepsilon}RI$. This mechanism may occur through $Fc{\varepsilon}RI$ expression inhibition.

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References

  1. Jung SM, Choi SI, Park SM, Heo TR. 2007. Antimicrobial effect of Achyranthes japonica Nakai extracts against Clostridium difficile. Korean J Food Sci Technol 39: 564-568.
  2. Marcone MF, Jahaniaval F, Aliee H, Kakuda Y. 2003. Chemical characterization of Achyranthes bidentata seed. Food Chem 81: 7-12. https://doi.org/10.1016/S0308-8146(02)00250-9
  3. Hahn DR, Lee MW. 1991. Studies on the constituents of Achyranthis Radix (I)-oleanolic acid bisdesmoside from the root-. Yakhak Hoeji 35: 457-460.
  4. Ida Y, Katsumata M, Satoh Y, Shoji J. 1994. Glucuronide saponins of oleanolic acid from Achyranthes fauriei roots. Planta Med 60: 286-287. https://doi.org/10.1055/s-2006-959481
  5. Bang SY, Kim JH, Kim HY, Lee YJ, Park SY, Lee SJ, Kim YH. 2012. Achyranthes japonica exhibits anti-inflammatory effect via $NF-{\kappa}B$ suppression and HO-1 induction in macrophages. J Ethnopharmacol 144: 109-117. https://doi.org/10.1016/j.jep.2012.08.037
  6. Kim JS, Lee SW, Kim SK, Na SW, Kim YO. 2014. Osteoprotective effect of extract from Achyranthes japonica in ovariectomized rats. J Exerc Rehabil 10: 372-377. https://doi.org/10.12965/jer.140169
  7. Lee SG, Lee EJ, Park WD, Kim JB, Kim EK, Choi SW. 2012. Anti-inflammatory and anti-osteoarthritis effects of fermented Achyranthes japonica Nakai. J Ethnopharmacol 142: 634-641. https://doi.org/10.1016/j.jep.2012.05.020
  8. Park JH, Kang SN, Shin D, Hur IC, Kim IS, Jin SK. 2013. Antioxidant activities of Achyranthes japonica Nakai extract and its application to the pork sausages. Asian-Australas J Anim Sci 26: 287-294. https://doi.org/10.5713/ajas.2012.12438
  9. Nolte H, Backer V, Porsbjerg C. 2001. Environmental factors as a cause for the increase in allergic disease. Ann Allergy Asthma Immunol 87: 7-11. https://doi.org/10.1016/S1081-1206(10)62333-2
  10. Kinet JP, Blank U, Brini A, Jouvin MH, Kuster H, Mejan O, Ra C. 1991. The high-affinity receptor for immunoglobulin E: a target for therapy of allergic diseases. Int Arch Allergy Appl Immunol 94: 51-55. https://doi.org/10.1159/000235323
  11. Beaven MA, Rogers J, Moore JP, Hesketh TR, Smith GA, Metcalfe JC. 1984. The mechanism of the calcium signal and correlation with histamine release in 2H3 cell. J Biol Chem 259: 7129-7136.
  12. Beaven MA, Metzger H. 1993. Signal transduction by Fc receptors: the $Fc{\varepsilon}RI$ case. Immunol Today 14: 222-226. https://doi.org/10.1016/0167-5699(93)90167-J
  13. Dombrowicz D, Flamand V, Brigman KK, Koller BH, Kinet JP. 1993. Abolition of anaphylaxis by targeted disruption of the high affinity immunoglobulin E receptor $\alpha$ chain gene. Cell 75: 969-976. https://doi.org/10.1016/0092-8674(93)90540-7
  14. Gauchat JF, Henchoz S, Mazzei G, Aubry JP, Brunner T, Blasey H, Life P, Talabot D, Flores-Romo L, Thompson J, Kishi K, Butterfield J, Dahinden C, Bonnefoy JY. 1993. Induction of human IgE synthesis in B cells by mast cells and basophils. Nature 365: 340-343. https://doi.org/10.1038/365340a0
  15. Metzger H. 1991. The high affinity receptor for IgE on mast cells. Clin Exp Allergy 21: 269-279. https://doi.org/10.1111/j.1365-2222.1991.tb01658.x
  16. Yanagihara Y, Kajiwara K, Basaki Y, Ikizawa K, Akiyama K, Saito H. 1997. Induction of human IgE synthesis in B cells by a basophilic cell line, KU812. Clin Exp Immunol 108: 295-301. https://doi.org/10.1046/j.1365-2249.1997.d01-1001.x
  17. Blank U, Ra C, Miller L, White K, Metzger H, Kinet JP. 1989. Complete structure and expression in transfected cells of high affinity IgE receptor. Nature 337: 187-189. https://doi.org/10.1038/337187a0
  18. Shim SY, Byun DS. 2011. Inhibitory effects of Chrysanthemum zawadsaki ethanolic extract on $Fc{\varepsilon}RI$ $\alpha$ chain expression. Korean J Food Sci Technol 43: 220-223. https://doi.org/10.9721/KJFST.2011.43.2.220
  19. Shim SY, Byun DS, Kim HR, Lee KD, Cho YS. 2006. Suppressive effects of Scutellaria radix water extract on $Fc{\varepsilon}RI$ expression. Food Sci Biotechnol 15: 694-697.
  20. Shim SY, Seo YK, Park JR. 2009. Down-regulation of $Fc{\varepsilon}RI$ expression by Houttuynia cordata Thunb extract in human basophilic KU812F cells. J Med Food 12: 383-388. https://doi.org/10.1089/jmf.2007.0684
  21. Shim SY, Sun HJ, Song YH, Kim HR, Byun DS. 2010. Inhibitory effects of blueberry root methanolic extract on degranulation in KU812F cells. Food Sci Biotechnol 19: 1185-1189. https://doi.org/10.1007/s10068-010-0169-9
  22. Kishi K. 1985. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leuk Res 9: 381-390. https://doi.org/10.1016/0145-2126(85)90060-8
  23. Shore PA, Burkhalter A, Cohn VH Jr. 1959. A method for the fluorometric assay of histamine in tissues. J Pharmacol Exp Ther 127: 182-186.
  24. Hakimi J, Seals C, Kondas JA, Pettine L, Danho W, Kochan J. 1990. The $\alpha$ subunit of the human IgE receptor ($Fc{\varepsilon}RI$) is sufficient for high affinity IgE binding. J Biol Chem 265: 22079-22081.
  25. Schwartz LB, Lewis RA, Seldin D, Austen KF. 1981. Acid hydrolases and tryptase from secretory granules of dispersed human lung mast cells. J Immunol 126: 1290-1294.
  26. Rivera J. 2002. Molecular adapters in $Fc{\varepsilon}RI$ signaling and the allergic response. Curr Opin Immunol 14: 688-693. https://doi.org/10.1016/S0952-7915(02)00396-5
  27. Rivera J, Fierro NA, Olivera A, Suzuki R. 2008. New insights on mast cell activation via the high affinity receptor for IgE. Adv Immunol 98: 85-120. https://doi.org/10.1016/S0065-2776(08)00403-3
  28. Miller L, Blank U, Metzger H, Kinet JP. 1989. Expression of high-affinity binding of human immunoglobulin E by transfected cells. Science 244: 334-337. https://doi.org/10.1126/science.2523561
  29. Ra C, Jouvin MH, Kinet JP. 1989. Complete structure of the mouse mast cell receptor for IgE ($Fc{\varepsilon}RI$) and surface expression of chimeric receptors (rat-mouse-human) on transfected cells. J Biol Chem 264: 15323-15327.
  30. Fujimura Y, Tachibana H, Yamada K. 2001. A tea catechin suppresses the expression of the high-affinity IgE receptor $Fc{\varepsilon}RI$ in human basophilic KU812 cells. J Agric Food Chem 49: 2527-2531. https://doi.org/10.1021/jf001392w
  31. Fujimura Y, Tachibana H, Maeda-Yamamoto M, Miyase T, Sano M, Yamada K. 2002. Antiallergic tea catechin, (-)-epigallocatechin-3-O-(3-O-methyl)-gallate, suppresses $Fc{\varepsilon}RI$ expression in human basophilic KU812 cells. J Agric Food Chem 50: 5729-5734. https://doi.org/10.1021/jf025680z
  32. Li Y, Lee SH, Le QT, Kim MM, Kim SK. 2008. Anti-allergic effects of phlorotannins on histamine release via binding inhibition between IgE and $Fc{\varepsilon}RI$. J Agric Food Chem 56: 12073-12080. https://doi.org/10.1021/jf802732n
  33. Shim SY, Choi JS, Byun DS. 2009. Kaempferol isolated from Nelumbo nucifera stamens negatively regulates $Fc{\varepsilon}RI$ expression in human basophilic KU812F cells. J Microbiol Biotechnol 19: 155-160. https://doi.org/10.4014/jmb.0804.259
  34. Shim SY, Choi JS, Byun DS. 2009. Inhibitory effects of phloroglucinol derivatives isolated from Ecklonia stolonifera on $Fc{\varepsilon}RI$ expression. Bioorg Med Chem 17: 4734-4739. https://doi.org/10.1016/j.bmc.2009.04.050