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Development of Modified Selective Media to Differentiate Cryptococcus Species Complex and its Serotypes using Natural Materials

  • Park, Gyu-Nam;Kim, Hye-Ran;An, Dong-Jun;Chae, Hee-Sun;Chang, Kyung-Soo
    • Biomedical Science Letters
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    • v.23 no.2
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    • pp.64-72
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    • 2017
  • The formation of brown colonies due to phenol oxidase activity on classic agar media containing natural material extracts of Helianthus annuus or on medium containing L-3,4-dihydroxyphenylalanine has been used to identify Cryptococcus species complex. In this study, various natural materials were used to develop a modified medium and to identify five major serotypes of Cryptococcus species complex. Serotypes A, D, and A/D were pigmented on medium using Perilla frutescens var. japonica Hara (PerJ agar) after a three-day incubation. Serotypes B and C were pigmented on PerJ agar after four- and five-day incubations, respectively. Growth time and pigmentation of the five serotypes occurred more rapidly on PerJ agar than on the other media. In addition, colony morphology, size, and pigmentation were specific by serotype. In conclusion, PerJ agar should be used in clinic settings to identify Cryptococcus species complex and its serotypes rapidly.

A Technology Planning Approach Based on Network and Growth Curve Analyses : the Case of Augmented Reality Patents (네트워크분석과 기술성장모형을 이용한 기술기획 : 증강현실 기술의 특허를 활용하여)

  • Kim, Jungwook;Jeong, Byeongki;Yoon, Janghyeok
    • Journal of Korean Institute of Industrial Engineers
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    • v.42 no.5
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    • pp.337-351
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    • 2016
  • As technologies' life-cycle shortens and their development directions are uncertain, firms' technology planning capability becomes increasingly important. Prior patent-based studies using technology growth curves identify developmental stages of technologies, thereby formulating technology development directions from an overall perspective. However, a technology generally consists of multiple sub-technologies and accordingly their development stages are likely various. In this regard, the prior studies failed to identify core sub-technologies and their specific development directions. Therefore, we suggest an approach consisting of 1) identifying core sub-technologies of a given technology using patent co-classifications and social network analysis, and 2) identifying each sub-technology's development stage and thereby determining its further development direction. We apply our approach to patents related to augmented reality to examine its applicability. It is expected that our approach will help identify evolving development stages for the core sub-technologies of a given technology, thereby effectively assisting technology experts in technology planning processes.

Quality of Life Related to Learning Style among Healthy Elderly (삶의 질과 학습유형의 관계: 건강한 노인을 대상으로)

  • Lee, Myung-Ok
    • The Journal of Korean Academic Society of Nursing Education
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    • v.12 no.1
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    • pp.21-27
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    • 2006
  • Purpose: This is a descriptive study to identify the relationship of quality of life and learning style of the elderly. Method: 106 elderly persons living in Seoul were surveyed using a questionnaire to identify their demographic characteristics, learning styles, and perceived quality of life. Results: 17% of the respondents were in the low quality of life (QOL). The QOL showed significantly different according to learning styles, gender, current health status, perceived level of current life happiness, and monthly pocket money. The highest average score of QOL was found in the Assimilator group, and the lowest average score was found in the Diverger group. Conclusions: Among the four categories significantly related to QOL, the case of learning style and current health status are the categories by which nurses can intervene to improve QOL. Thus, nurses should emphasize the relationship to improve the clients' QOL. Since the scores of QOL were high for the Assimilator and Accomodator groups, nurse should identify the learning style of the elderly as soon as possible and then help those who are under-developed, to further develop Assimilator and Accomodator learning styles.

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A Development of the Risk Identification Checklist through the Re-establishment of Risk Breakdown Structure of Construction Project (건설사업 위험분류체계의 재정립을 통한 위험인지 체크리스트 개발)

  • Chu Hae-Keum;Kim Seon-Gyoo
    • Korean Journal of Construction Engineering and Management
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    • v.4 no.2 s.14
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    • pp.109-117
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    • 2003
  • The construction project is largely exposed to much more risk events over the project life cycle due to its complexity and size than the other industries. Therefore, the construction risk management process to identify and response the risk events is not only performing acutely but also proceeding systematically. The risk identification phase in the risk management process is to identify various risk events and define its characteristics. At this phase, the risk identification system is very useful tool to identify every possible risk events in the project. This study shows some problems of the existing risk identification system and proposes the modified risk identification system based on the project phases and the contract bodies, and also suggests partial but more enough detailed risk checklists to be implemented in the actual risk identification phase than any other existing risk breakdown systems to be examined at this study.

Positional Cloning and Phenotypic Characterization of a New Mutant Mouse with Neuronal Migration Abnormality

  • Park, Chankyu;Ackerman, Susan-L
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2001.10a
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    • pp.14-17
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    • 2001
  • Positional clonging (map-based cloning) of mutations or genetic variations has been served as an invaluable tool to understand in-vivo functions of genes and to identify molecular components underlying phenotypes of interest. Mice homozygous for the cerebellar deficient folia (cdf) mutation are ataxic, with cerebellar hypoplasia and abnormal lobulation of the cerebellum. In the cdf mutant cerebellum approximately 40% of Purkinje cells are ectopically located within the white matter and the inner granule cell layer (IGL). To identify the cdf gene, a high-resolution genetic map for the cdf-gene-encompassing region was constructed using 1997 F2 mice generated from C3H/HeSnJ-cdf/cdf and CAST/Ei intercross. The cdf gene showed complete linkage disequilibrium with three tightly linked markers D6Mit208, D6Mit359, and D6Mit225. A contig using YAC, BAC, and P1 clones was constructed for the cdf critical region to identify the gene. A deletion in the cdf critical region on chromosome 6 that removes approximately 150 kb of DNA selection. cdf mutant mice with the transgenic copy of the identified gene restored the brain abnormalities of the mutant mice. The positional cloning of cdf gene provides a good example showing the identification of a gene could lead to finding a new component of important molecular pathways.

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A Study on the Effect of PPL Models on Fashion Brand Attitude (PPL 광고(廣告)모델이 의류(衣類)브랜드 태도(態度)에 미치는 영향(影響)에 관(關)한 연구(硏究))

  • Kim, Il;Kweon, Mi-Kyoung
    • Journal of Fashion Business
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    • v.7 no.1
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    • pp.1-13
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    • 2003
  • By considering the influences of PPL(Product Placement) commercial model on consumers' attitude to clothing brand as a commercial effect of PPL, this study intends to identify how much differently they take an attitude to clothing brands before and after exposed to PPL commercials, and also identify how much differently they take their attitude to the brands depending upon PPL commercial models. In the step of experimental design, a previous survey was performed to identify how consumers take their attitude to commercial models and brands before exposed to PPL commercials. And every subject was asked to watch video films with brand logos exposed. The results of experiment can be summarized as follows: After all subjects were exposed to PPL commercials, in general, their attitude to commercial models had more or less effects on the change of their attitude. Meanwhile, the interaction between their attitude to model and clothing brands respectively had somewhat significant influences only on brand recognition. This study verified that PPL commercial models had significant influences on consumers' attitude to brands. These results imply that a PPL commercial strategy in fashion industry should be made focusing on the respective roles of PPL commercial models.

Identification of Differentially Expressed Genes (DEGs) by Malachite Green in HepG2 Cells

  • Kim, Youn-Jung;Song, Mee;Ryu, Jae-Chun
    • Molecular & Cellular Toxicology
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    • v.4 no.1
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    • pp.22-30
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    • 2008
  • Malachite Green (MG), a toxic chemical used as a dye, topical antiseptic and antifungal agent for fish, is highly soluble in water, cytotoxic to various mammalian cells and also acts as a liver tumor promoter. In view of its industrial importance and possible exposure to human beings, MG possesses a potential environmental health hazard. So, we performed with HepG2, a human hepatocellular carcinoma cell line, to identify the differentially expressed genes (DEGs) related to toxicity of MG. And we compared gene expression between control and MG treatment to identify genes that are specifically or predominantly expressed by employing annealing control primer (ACP)-based $GeneFishing^{TM}$ method. The cytotoxicity $(IC_{20})$ of MG was determined above the $0.867{\mu}M$ in HepG2 cell for 48 h treatment. And the DEGs of MG were identified that 5 out of 6 DEGs were upregulated and 1 out of 6 DEGs was down-regulated by MG. Also, MG induced late apoptosis and necrosis in a dose dependent in flow cytometric analysis. Through further investigation, we will identify more meaningful and useful DEGs on MG, and then can get the information on mechanism and pathway associated with toxicity of MG.

Use Studies of Library Collections (장서평가에 관한 소고 -특히 이용조사를 중심으로-)

  • Yoo Chae-Ock
    • Journal of the Korean Society for Library and Information Science
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    • v.15
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    • pp.175-195
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    • 1988
  • Use studies of library collections have been conducted as a method of evaluating collections in a library. The main purpose of use studies is to evaluate the quality of a library collection in terms of extent and mode of its use. In addition to use studies, both quantitative and qualitative methods could be utilized in order to evaluate a library collection. However, the quantitative and qualitative collection evaluation methods are more concerned with the collection itself than with its use. Use studies have been conducted in large academic libraries for the following specific purposes: 1) They attempt to identify little used portion of collections that can be retired less accessible and less expensive storage area. 2) They try to identify core collections to satisfy some degree of circulation demands in the near future. 3) They try to identify use patterns of selected subject areas or type of books that can be used to adjusting collection development practices or fund allocations. 4) They try to assess the document delivery capability of a library to improve their availability. A number of methodologies employed for these specific purposes fall into four major categories; 1) circulation analysis method, 2) last circulation method, 3) relative use method, and 4) document delivery test. Each method is briefly reviewed with its limitations.

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A Study on the Emoticon Extraction based on Facial Expression Recognition using Deep Learning Technique (딥 러닝 기술 이용한 얼굴 표정 인식에 따른 이모티콘 추출 연구)

  • Jeong, Bong-Jae;Zhang, Fan
    • Korean Journal of Artificial Intelligence
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    • v.5 no.2
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    • pp.43-53
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    • 2017
  • In this paper, the pattern of extracting the same expression is proposed by using the Android intelligent device to identify the facial expression. The understanding and expression of expression are very important to human computer interaction, and the technology to identify human expressions is very popular. Instead of searching for the emoticons that users often use, you can identify facial expressions with acamera, which is a useful technique that can be used now. This thesis puts forward the technology of the third data is available on the website of the set, use the content to improve the infrastructure of the facial expression recognition accuracy, in order to improve the synthesis of neural network algorithm, making the facial expression recognition model, the user's facial expressions and similar e xpressions, reached 66%.It doesn't need to search for emoticons. If you use the camera to recognize the expression, itwill appear emoticons immediately. So this service is the emoticons used when people send messages to others, and it can feel a lot of convenience. In countless emoticons, there is no need to find emoticons, which is an increasing trend in deep learning. So we need to use more suitable algorithm for expression recognition, and then improve accuracy.

A Study on the Noise and Vibration Path of Hermetic Rotary Compressor by SEA (통계적 에너지 해석 기법에 의한 밀폐형 회전 압축기의 소음진동 전달경로 해석)

  • Hwang, Seon-Woong;Ahn, Byung-Ha;Jeong, Hyeon-Chul;Jeong, Weui-Bong;Kim, Kyu-Hwan
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2002.11b
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    • pp.869-874
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    • 2002
  • Hermetic rotary compressor is one of the most important components for air conditioning system since it has a great effect on both the performance and the noise and vibration of the system. Noise and vibration of rotary compressor is occurred due to gas pulsation during compression process and unbalanced dynamic force. In order to reduce noise and vibration, It is necessary to identify sources of noise and vibration and effectively control them. Many approaches have been tried to identify noise sources of compressor. However, compressor noise source identification has proven to be difficult since the characteristics of compressor noise are complicated due to the interaction of the compressor parts and gas pulsation. In this work, Statistical Energy Analysis has been used to trace the energy flow in the compressor and identify transmission paths from the noise source to the sound field.

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