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Whole Genome Resequencing of Heugu (Korean Black Cattle) for the Genome-Wide SNP Discovery

  • Choi, Jung-Woo;Chung, Won-Hyong;Lee, Kyung-Tai;Choi, Jae-Won;Jung, Kyoung-Sub;Cho, Yongmin;Kim, Namshin;Kim, Tae-Hun
    • Food Science of Animal Resources
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    • v.33 no.6
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    • pp.715-722
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    • 2013
  • Heugu (Korea Black Cattle) is one of the indigenous cattle breeds in Korea; however there has been severe lack of genomic studies on the breed. In this study, we report the first whole genome resequencing of Heugu at higher sequence coverage using Illumina HiSeq 2000 platform. More than 153.6 Giga base pairs sequence was obtained, of which 97% of the reads were mapped to the bovine reference sequence assembly (UMD 3.1). The number of non-redundantly mapped sequence reads corresponds to approximately 28.9-fold coverage across the genome. From these data, we identified a total of over six million single nucleotide polymorphisms (SNPs), of which 29.4% were found to be novel using the single nucleotide polymorphism database build 137. Extensive annotation was performed on all the detected SNPs, showing that most of SNPs were located in intergenic regions (70.7%), which is well corresponded with previous studies. Of the total SNPs, we identified substantial numbers of non-synonymous SNPs (13,979) in 5,999 genes, which could potentially affect meat quality traits in cattle. These results provide genome-wide SNPs that can serve as useful genetic tools and as candidates in searches for phenotype-altering DNA difference implicated with meat quality traits in cattle. The importance of this study can be further pronounced with the first whole genome sequencing of the valuable local genetic resource to be used in further genomic comparison studies with diverse cattle breeds.

Improving the Gravity Model for Feasibility Studies in the Cultural and Tourism Sector (문화·관광부문 타당성조사를 위한 중력모형의 개선방안)

  • Hae-Jin Lee
    • Asia-Pacific Journal of Business
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    • v.15 no.1
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    • pp.319-334
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    • 2024
  • Purpose - The purpose of this study is to examine the gravity model commonly used for demand forecasting upon the implementation of new tourist facilities and analyze the main causation of forecasting errors to provide a suggestion on how to improve. Design/methodology/approach - This study first measured the errors in predicted values derived from past feasibility study reports by examining the cases of five national science museums. Next, to improve the predictive accuracy of the gravity model, the study identified the five most likely issues contributing to errors, applied modified values, and recalculated. The potential for improvement was then evaluated through a comparison of forecasting errors. Findings - First, among the five science museums with very similar characteristics, there was no clear indication of a decrease in the number of visitors to existing facilities due to the introduction of new facilities. Second, representing the attractiveness of tourist facilities using the facility size ratio can lead to significant prediction errors. Third, the impact of distance on demand can vary depending on the characteristics of the facility and the conditions of the area where the facility is located. Fourth, if the distance value is below 1, it is necessary to limit the range of that value to avoid having an excessively small value. Fifth, depending on the type of population data used, prediction results may vary, so it is necessary to use population data suitable for each latent market instead of simply using overall population data. Finally, if a clear trend is anticipated in a certain type of tourist behavior, incorporating this trend into the predicted values could help reduce prediction errors. Research implications or Originality - This study identified the key factors causing prediction errors by using national science museums as cases and proposed directions for improvement. Additionally, suggestions were made to apply the model more flexibly to enhance predictive accuracy. Since reducing prediction errors contributes to increased reliability of analytical results, the findings of this study are expected to contribute to policy decisions handled with more accurate information when running feasibility analyses.

Floristic Characteristics of Vascular Plants in the Maryong-san(Mt. 406.7m) and Daeso-san(Mt. 286m) Area (마룡산(406.7m, 울진군)과 대소산(286m, 영덕군) 일원의 관속식물)

  • Kim, Young-Chul;Chae, Hyun-Hee;Park, You-Cheol;Lee, Seon-Mi;Nam, Gi-Heum
    • Korean Journal of Environment and Ecology
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    • v.35 no.5
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    • pp.438-479
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    • 2021
  • Basic information such as flora and their distribution are the key factors to consider for preserving plant diversity on a regional level. The number of plant species identified through a survey is closely related to the survey area and the number of habitats distributed within the survey area. In this study, we surveyed the flora of Mt. Maryong-san (PH) and Mt. Daeso-san (YH) located in Uljin-gun, Gyeongsangbuk-do, six and nine times, respectively. We planned to include a variety of habitats in the survey routes. As a result, we identified a total of 594 taxa composing of 112 families, 349 genera, 548 species, 10 subspecies, 34 varieties, and 2 formulas in the Mt. Maryong-san (PH) area and a total of 434 taxa composing of 89 families, 270 genera, 400 species, 9 subspecies, and 25 varieties in the Mt. Daeso-san (YH) area. Of the plants observed in the 21 habitats, the plants distributed in the transition zone around the valley and the dry grassland accounted for the highest proportion. The traditional graveyards located on the coast also served as the major habitat for the distribution of some plant species. In the two graveyards, 19 taxa of endemic plant species were surveyed. Moreover, the identified floristic target species included 1 taxon for grade V, 11 taxa for grade IV, and 24 taxa for grade III. A total of 77 taxa of naturalized plants were identified, and four of them were invasive alien plants as designated by the Ministry of Environment. The differences in the plants distributed in the area of Mt. Maryong-san (PH) and Mt. Daeso-san (YH) were mainly due to the difference in the size of each habitat distributed within the survey area. Moreover, it was judged that the entire vicinity of Mt. Daeso-san (YH) was not connected to the surrounding mountains and was an isolated area surrounded by farmland and residential areas.

The Vascular Plants in Mt. Gilsangsan (Ganghwa-Isl.), Korea (길상산 (강화도)의 관속식물상)

  • Kim, Jung-Hyun;Kim, Sun-Yu
    • Korean Journal of Environment and Ecology
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    • v.27 no.3
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    • pp.280-304
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    • 2013
  • This study was carried out to investigate the flora of Mt. Gilsangsan (Ganghwa-Isl.). The vascular plants were collected 14 times (from March to November 2012), and were identified as 595 taxa; 122 families, 359 genera, 529 species, 7 subspecies, 51 varieties, 2 forms and 3 hybrids. The plant formation of Mt. Gisangsan is the deciduous broad-leaved and conifer mixed forest which is the common one in the middle part of Korean peninsula. All most mountain covered with young secondary forest which is mainly composed of Pinus and Quercus. The family represented by the largest number of species diversity were Asteraceae (11.%), Poaceae (10.7%), Cyperaceae (6.38%), Fabaceae (3.86%) and Rosaceae (3.52%) respectively. Korean endemic plants of this area were 12 taxa; Asarum misandrum, Clematis brachyura, Philadelphus schrenkii, Forsythia koreana, Polygonatum infundiflorum, etc. The red list of vascular plants according to IUCN valuation basis were examined, endangered species (EN) Abies koreana, Near Threatened species (NT) both Wikstroemia trichotoma and Sparganium erectum, Least Concern species (LC) Platycladus orientalis, Swertia diluta var. tosaensis and Iris minutoaurea and by Not Evaluate species (NE) Polygonatum infundiflorum. The floristic regional indicator plants found in this area were 45 taxa comprising two taxa of grade V and VI, nine taxa of grade III, three taxa of grade II, 29 taxa of gradeI. The naturalized plants were identified as 64 taxa and the percent of naturalized index (NI) was 10.7% of total 595 taxa vascular plants. Ecosystem disturbance wild plants were consisted of eight taxa; Rumex acetosella, Sicyos angulatus, Solanum carolinense, Ambrosia artemisiifolia, Ambrosia trifida, Aster pilosus, Hypochaeris radicata and Lactuca scariola. Furthermore, therophytes (26.5%), hemicryptophytes (26.0%), Geophyte (14.0%) showed high proportional ratio in life form spectrum.

A Efficient Architecture of MBA-based Parallel MAC for High-Speed Digital Signal Processing (고속 디지털 신호처리를 위한 MBA기반 병렬 MAC의 효율적인 구조)

  • 서영호;김동욱
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.41 no.7
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    • pp.53-61
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    • 2004
  • In this paper, we proposed a new architecture of MAC(Multiplier-Accumulator) to operate high-speed multiplication-accumulation. We used the MBA(Modified radix-4 Booth Algorithm) which is based on the 1's complement number system, and CSA(Carry Save Adder) for addition of the partial products. During the addition of the partial product, the signed numbers with the 1's complement type after Booth encoding are converted in the 2's complement signed number in the CSA tree. Since 2-bit CLA(Carry Look-ahead Adder) was used in adding the lower bits of the partial product, the input bit width of the final adder and whole delay of the critical path were reduced. The proposed MAC was applied into the DWT(Discrete Wavelet Transform) filtering operation for JPEG2000, and it showed the possibility for the practical application. Finally we identified the improved performance according to the comparison with the previous architecture in the aspect of hardware resource and delay.

Study of the Activation Plan for Rural Tourism of the Jeollabuk-do Using Big Data Analysis (빅데이터 분석을 통한 농촌관광 실태와 활성화 방안 연구: 전라북도를 중심으로)

  • Park, Ro Un;Lee, Ki Hoon
    • The Korean Journal of Community Living Science
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    • v.27 no.spc
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    • pp.665-679
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    • 2016
  • This study examined the main factors for activating rural tourism of Jeollabuk-do using big data analysis. The tourism big data was gathered from public open data sources and social network services (SNS), and the analysis tools, 'Opinion Mining', 'Text Mining', and 'Social Network Analysis(SNA)' were used. The opinion mining and text mining analysis identified the key local contents of the 14 areas of Jeollabuk-do and the evaluations of customers on rural tourism. Social network analysis detected the relationships between their contents and determined the importance of the contents. The results of this research showed that each location in Jeollabuk-do had their specific contents attracting visitors and the number of contents affected the scale of tourists. In addition, the number of visitors might be large when their tourism contents were strongly correlated with the other contents. Hence, strong connections among their contents are a point to activate rural tourism. Social network analysis divided the contents into several clusters and derived the eigenvector centralities of the content nodes implying the importance of them in the network. Tourism was active when the nodes at high value of the eigenvector centrality were distributed evenly in every cluster; however the results were contrary when the nodes were located in a few clusters. This study suggests an action plan to extend rural tourism that develop valuable contents and connect the content clusters properly.

Sex-related demographics in two remnant populations of a dioecious tree Ilex cornuta (Aquifoliaceae): implications for conservation

  • Shin, Sookyung;Lee, Hakbong;Lee, Jei-Wan;Kang, Hyesoon
    • Journal of Ecology and Environment
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    • v.43 no.3
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    • pp.320-331
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    • 2019
  • Background: Dioecious plant species having both male and female plants have been investigated regarding sex-related characteristics such as sex ratio, sex-differential resource requirements, and spatial segregation of the sexes. Habitat loss and fragmentation are major threats to the survival of plant populations, but dioecious species are particularly more prone to such habitat degradation than non-dioecious species because of their dimorphic sexual system. We examined the sex-related demographics of two Ilex cornuta populations being different regarding land use history. Methods: During 2016-2017, we examined I. cornuta trees with a basal diameter ${\geq}1.5cm$ in the Yongsu-ri population (YS population) and the Gotjawal Provincial Park population (GP population). Plant sex (male, female, or unsexed) was identified. The tree size (basal diameter and height of the main stem), clonal production (the ramet numbers per genet), and vitality for each clone were measured. The associations between population, sex, tree size, clonal production, and vitality were examined using ANOVAs and contingency table analyses. Finally, point pattern analyses using O-ring statistics were conducted to assess spatial patterns. Results: Upon excluding unsexed trees, the YS population with 74 trees was significantly male-biased (0.66), while the GP population with only 26 trees had a 1:1 sex ratio. In both populations, males and females did not differ in tree size. Although the mean number of ramets differed significantly between populations, females tended to produce more ramets than males. The proportion of weak trees was significantly higher in the YS than in the GP population. Neither population showed evidence of spatial segregation of the sexes. Conclusions: The two populations of dioecious I. cornuta are characterized by the small number of trees and relatively high frequencies of non-reproductive trees. Both indicate that these populations are quite susceptible to environmental and genetic stochasticity. On the other hand, the differences between populations in sex ratio, clonal production, and vitality suggest that conservation efforts for I. cornuta need to be population-specific. In order to help recover and enable this vulnerable species to persist, it is necessary to find ways to enhance their sexual reproduction and simultaneously reduce habitat disturbances due to anthropogenic activities.

Morphological, Cytological and Molecular Evidence for Intersubgeneric F1 Hybrid between Glycine max x G. tomentella (콩 Glycine max와 G. tomentella의 종간교잡으로부터 얻은 Fl식물체 검증을 위한 형태적 · 세포학적 · 분자유전학적 연구)

  • Choi, In-Soo;Kim, Yong-Chul
    • Journal of Life Science
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    • v.18 no.4
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    • pp.454-460
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    • 2008
  • This study was carried out to demonstrate morphological, cytological and molecular evidence for intersubgeneric $F_1$ hybrid between Glycine tomentella and G. max cv. ‘Baemkong’. Morphological features of $F_1$ plant for pistil and stamen, flower color and growth habit showed intermediate type between G. tomentella and G. max cv. ‘Baemkong’. Chromosome number of $F_1$ plant was 2n=39, which explained the evidence of $F_1$ hybrid between G. tomentella (2n=38) and G. max cv. ‘Baemkong’ (2n=40). Polyacrylamide isoelectric focusing pattern for esterase and peroxidase also showed that the $F_1$ plant was true $F_1$ hybrid between G. tomentella and G. max cv. ‘Baemkong’. From RAPD analysis, we identified that 62 primers showing bands in $F_1$ hybrid had both bands from G. tomentella and G. max cv. ‘Baemkong’, which suggested that this was true $F_1$ hybrid. Based on our results from morphological, cytological and molecular analyses, we suggest that the $F_1$ plant was true intersubgeneric hybrid between G. tomentella and G. max cv. ‘Baemkong’. Our results still remain us further study to recover fertility of $F_1$ hybrids. The occurrence of maternal and/or paternal inheritance in $F_1$ hybrid from intersubgeneric cross between G. tomentella and G. max cv. ‘Baemkong’ need to be explained.

The Effect of Organic Fertilizer According to the Duration and Amount of Soil Chemical Changes on Yield Components of Rice (유기질비료의 사용기간과 사용량에 따른 토양화학성변화와 벼의 수량구성요소에 미치는 영향)

  • Oh, Tae-Seok;Kim, Sung-Min;Kim, Chang-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.3
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    • pp.209-215
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    • 2014
  • The purpose of this study is identify chemical changes in yield components of soil using organic fertilizer for cultivating rice and establish reasonable methods of fertilization. In chemical change, comparing the control plot, the soil using organic fertilizer consistently for three years for cultivating rice showed an increase in pH and available phosphate in the experiment plots provided by the organic fertilizer, increase in spikelet number and 1000 grain weight in terms of yield components, and increase in yield in the three-year experiment plots. As for the quality of brown rice, the rate of head rice showed no difference when compared to that of the control plots. As for protein content, it was difficult to identify a statistical significance between the control plots and the experiment plots in 2009, but since 2010, the protein content of the experiment plots has been lower than that of the control plot, indicating that fertilization of organic fertilizer was effective in terms of rice quality. In conclusion, long-term use of organic fertilizer improved the chemical properties of soil and increased yield when compared to practical fertilization. Reasonable fertilization was identified to fertilize the nitrogen component of organic fertilizer to be a level of 12kg/10a.

Distribution and Classification of Cordyceps Parasites in Cicada (매미를 기주로 한 동충하초의 분포와 분류 동정)

  • Oh, Junsang;Han, Sang-Kuk;Shrestha, Bhushan;Sung, Gi-Ho;Sung, Jae-Mo
    • Journal of Naturopathy
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    • v.8 no.2
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    • pp.78-87
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    • 2019
  • Purpose: The aim was to collect and classify the Cordyceps parasitized in cicadas from July to October every year from 1990 to 2016 in Korea. And they were frequently collected in Nepal, Vietnam, Japan, China, and Thailand. Methods: Cordyceps parasitizing cicadas collected in mountains and fields. Results: A total of 1,104 specimens were collected that belonged to 10 different species under nine genera. The highest number of samples belonged to Ophiocordyceps (563 specimens), followed by Isaria (361 specimens), Polycephalomyces (73 specimens), Cordyceps (70 specimens), Beauveria (25 specimens), Perennicordyceps (8 specimens), Metarhizium (2 specimens) and Purpureocillium (2 ones). Among Ophiocordyceps spp. O. longissima was most frequently collected with a total of 426 samples out of 563, followed by O. heteropoda with 120 ones and O. sobolifera with 17 specimens. The species mainly collected in Korea, but C. ishikariensis was collected in Nepal only. The new characteristic was that Isaria cicada-like synnemata were found growing together with C. ishikariensis stromata on the same host. In Korea, the collected 691 specimens in total out of 1,104 were found in Mt. Halla in Jeju Island. Other mountains in Korea where the samples were collected were Mountains Daeryong, Jiri, Yongmoon, Samag, Seolag, Gujeol, Duryun, Baegam, Chilgap, Chundeung, Naejang, Welchul, and Daeryong. The three samples were not identified. Conclusions: A total of 1,104 specimens belonged to 10 different species under nine genera, and the collected 691 samples were found in Mt. Halla in Jeju Island.