• Title/Summary/Keyword: Identification characters

Search Result 296, Processing Time 0.024 seconds

Numerical Identification of a Streptomyces Strain Producing a Thermotolerable Restriction endonuclease Svi I (내열성 제한효소인 Svi I을 생성하는 Streptomyces 분리균주의 수리 동정)

  • Bae, Moo;Yun, Mi-Sub;Kim, Hyoung-Tae;Lee, Kye-Joon
    • Microbiology and Biotechnology Letters
    • /
    • v.21 no.4
    • /
    • pp.299-305
    • /
    • 1993
  • Numerical identification was carried out for an isolate of Streptomyces D2-5 producing a new restriction endonuclease Svi I. Fifty taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate was best matched to Streptomyces violochromogenes in the major cluster 18 of Streptomyces. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces violochromogenes.

  • PDF

Numerical Identification of an Actinomycetes Strain Producing an Antitumor Antibiotic with Inhibitory Activity against DNA Topoisomerase (DNA topoisomerase I Inhibitor 를 생성하는 방선균 분리균주의 수리동정)

  • Lee, Dong-Sun;Ha, Sang-Chul;Shin, Woo-Chang;Kim, Tae-Ho;Kim, Hong-Joong;Park, Yong-Ha;Kim, Jong-Guk;Hong, Soon-Duck
    • Microbiology and Biotechnology Letters
    • /
    • v.23 no.2
    • /
    • pp.123-130
    • /
    • 1995
  • DNA topoisomerase I have been shown to be important therapeutic target in cancer chemotherapy. Chemotaxonomy and numerical identification were carried out for an isolate strain No.7489 producing an antibiotic that inhibits DNA topoisomerase I activity. The genus of strain No.7489 was determined as Streptomyces sp. from culture, morphological and chemotaxonomic data. Thirty-nine taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate was best matched to Streptomyces melanosporofaciens in the major cluster 32 of Streptomyces. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces melanosporofaciens.

  • PDF

Isolation and Identification of Streptomyces chromofuscus Producing Cathepsin B Inhibitor (Cathepsin B 저해물질을 생산하는 Streptomyces chromofuscus의 분리 및 동정)

  • Lee, Hyoun Suk;Kim, In Seop;Kim, Hyoung Tae;Yoon, Sung Joon;Lee, Kye Joon
    • Microbiology and Biotechnology Letters
    • /
    • v.23 no.5
    • /
    • pp.565-572
    • /
    • 1995
  • The aim of the present research program was to develop a strain of actinomycetes producing extracellular cathepsin B inhibitor. Soil samples were collected from various sites in Korea and a number of actinomycetes were isolated from the soil samples by applying various physical and chemical pretreatments. An economical and effective method was developed for the screening of strains producing low molecular weight cathepsin B inhibitor, and consequently a strain (SMF28) among over 700 isolates was selected. Chemotaxonomic and numerical identification were carried out for the isolate. Fifty taxonomic unit characters were tested and the data were analyzed numerically using TAXON program. The isolate was identified as a strain of Streptomyces chromofuscus.

  • PDF

A Identification of Tire Moldnumber using 3 Dimension Data (3차원 데이터를 이용한 타이어 몰드번호 추출 및 인식)

  • Lee, Ki Seong;Jeong, Tae Won
    • The Transactions of the Korean Institute of Electrical Engineers D
    • /
    • v.54 no.10
    • /
    • pp.595-603
    • /
    • 2005
  • This Paper proposes the tire mold number identification method which reads the letters on a tire surface with 3D. It is very difficult to separate the letters from the background of an image since the letters on a tire surface is an embossed data. There was many studies to read the letters on a tire surface for the factory automation, however, it was very difficult to separate the letters from the background of an image since the letters on a tire surface is embossed black characters on the black ground. In this study, we first developed the method to find the location of tire mold number, which is used to classify the tire size, from the embossed letters on a tire surface using 3-dimensional laser profile camera which is not affected by the lighting condition, then developed the method to separate the mold number from that location. As a result, we were able to contribute to automate the tire size classification which has been manually performed by operators previously.

Numerical Identification of a Streptomyces Strain Producing Spores in Submerged Culture

  • Rho, Yong-Taik;Kim, Hyoung-Tae;Oh, Kyoung-Hee;Kang, Heui-Il;Alan C. Ward;Michael Goodfellow;Hah, Yung-Chil;Lee, Kye-Joon
    • Korean Journal of Microbiology
    • /
    • v.30 no.4
    • /
    • pp.278-285
    • /
    • 1992
  • Chemotaxonomic and numerical identification were carried out for a isolate of Streptomyces strain SMF301 producing spores in submerged culture. Fifty taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate SMF301 was identified to cluster 1A of Streptomyces and best matched to Streptomyces limosus which is a synonym of Streptomyces albidoflavus. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces alidoflavus.

  • PDF

Numerical Identification of a Streptomyces Strain Producing Thiol Protease Inhibitor

  • Lee, Kye-Joon;Kim, In-Seop;Kim, Hyoun-Tae;Ward, Alan-C.;Goodfellow, Michael
    • Journal of Microbiology and Biotechnology
    • /
    • v.2 no.3
    • /
    • pp.220-225
    • /
    • 1992
  • Chemotaxonomic and numerical identification were carried out for an isolate of Streptomyces strain SMF13 producing thiol protease inhibitor. Fifty taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate SMF13 was identified to be a member of the cluster 5 of Streptomyces and best matched to Streptomyces omiyaensis which is a synonym of Streptomyces exfoliatus. Therefore. it was concluded that the isolate was identified to be a strain of Streptomyces exfoliatus.

  • PDF

Numerical Identification of a Streptomyces Strain Producing Restriction Endonuclease Sdi I (제한효소인 Sdi I을 생성하는 Streptomyces 분리 균주의 수리동정)

  • Bae, Moo;Suh, Won-Na;Song, Eun-Sook;Lee, Kye-Joon
    • Microbiology and Biotechnology Letters
    • /
    • v.22 no.2
    • /
    • pp.126-133
    • /
    • 1994
  • Numerical identification was aplied for Streptomyces sp.264, an isolate producing a new restriction endonuclease Sdi I. The restriction enzyme would appear to be an isoschisomer of Xho I. Fifty taxonomic unit characters were tested and the data obtained were analyzed numerically by using the TAXON program. The isolate was identified to be the major cluster 19 of Streptomyces and best matched to S. diastatochromogenes. It was, therefore, concluded that the isolate was identified to be a member of Streptomyces diastatochromogenes.

  • PDF

Numerical Identification of an Actiomycetes Stain Producing an Antibiotic that Generates Oxygen Radical (활성산소를 발생하는 항생물질을 생성하는 방선균 분리균주의 수리동정)

  • Lee, Dong-Sun;Ha, Sang-Chul;Shin, Woo-Chang;Kim, Tae-Ho;Kim, Hong-Joong;Park, Yong-Ha;Kim, Jong-Guk;Hong, Soon-Duck
    • Microbiology and Biotechnology Letters
    • /
    • v.23 no.4
    • /
    • pp.397-404
    • /
    • 1995
  • Chemotaxonomy and numerical identification were carried out for an isolate strain No.8001 producing an antibiotic that generates oxygen radical. The genus of strain No.8001 was decided as Streptomyces sp. from chemotaxonomic data. Thirty-nine taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate was best matched to Streptomyces citreofluorescens in the major cluster 1B of Streptomyces. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces citreofluorescens.

  • PDF

Research about application of Shanghanlun disease pattern identification diagnostic system to 2 cases treated by Oreong-san (오령산(五苓散)을 활용한 2개 증례 분석을 통한 『상한론(傷寒論)』 변병진단체계(辨病診斷體系) 응용에 관한 연구)

  • Park, Jae-kyung;Lim, Eun-Kyo
    • 대한상한금궤의학회지
    • /
    • v.10 no.1
    • /
    • pp.81-94
    • /
    • 2018
  • Objective : The objective of this study is to define the clinical meaning of Shanghanlun sentence by applying Shanghanlun disease pattern identification diagnostic system. Methods : 2 clinical cases, which were treated by Oreong-san according to Shanghanlun provisions dualized with Je-Gang(提綱) and Jo-Moon(條文), were analyzed. Results : According to the results of analysis of 2 cases, the patients' diseases were improved. The pain was decreased, and general weakness and anorexia were improved in 2 cases. Conclusions : These results suggests that the medicines from Shanghanlun are clinically effective when Shanghanlun is interpreted considering the origins of Chinese characters in the text, and Shanghanlun disease pattern identification diagnostic system is applied.

Studies on the Identification of Turfgrass by Electrophoresis (SDS-PAGE, PAGIF) (전기영동법 (SDS-PAGE, PAGIF)에 의한 잔디 분류에 관한 연구)

  • 박재복;김영후;이수영
    • Asian Journal of Turfgrass Science
    • /
    • v.5 no.1
    • /
    • pp.11-22
    • /
    • 1991
  • This experiment was executed to investigate the possibility of the application of taxonomic method through the isoelectric focusing with polyacrylamide gel and sodium dodecyl sulfate-polyacrylamide gel electrophoresis with seeds in the identification of turfgrasses. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to investigate the pattern of seed proteins which were extracted from 18 cultivars of cool season turfgrass and 4 cultivars of warm season turfgrass. The isoelectric focusing with polyacrylarnide gel was used to investigate the activity of the three isozymes of esterase, peroxidase and phosphoglucose isomerase which were extracted from 18 cultivars of cool season turfgrass and 4 cultivars of warm season turfgrass. The results were summarized as follows. 1. The difference of the patterns of seed proteins was observed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The identification of intra-genus was easily detected. 2. The three isozymes of esterase, peroxidase and phosphoglucose isomerase were investigated through isoelectric focusing with polyacrylamide gel. As a result, esterase was most effective among three isozymes in the identification of turfgrass cultivars 3. In the past cultivar identification was primarily based on visual morphological characters, but there was a lot of difficulty. If we should use electrophoresis, we will be able to identifvturfgrass cultivars more effectively.

  • PDF