• Journal of Microbiology and Biotechnology
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• v.16 no.6
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• pp.901-910
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• 2006

Poly(3-hydroxybutyrate) [P(3HB)] is a microbial polyester intracellularly accumulated as distinct granules in numerous microorganisms as an energy and carbon storage material. Recombinant Escherichia coli harboring the heterologous P(3HB) biosynthesis genes accumulates large amounts of P(3HB) granules, yet the granule-associated proteins have not been identified. Therefore, this study reports on an analysis of the P(3HB) granule-associated proteome in recombinant E. coli. Fiye proteins out of 7 spots identified were found to be involved in functions of translation, heat-stress responses, and P(3HB) biosynthesis. Two of the major granule-associated proteins, IbpA/B, which are already known to bind to recombinant proteins forming inclusion bodies in E. coli, were further analyzed. Immunoblotting and immunoelectron microscopic studies with IbpA/B antibodies clearly demonstrated the binding and localization of IbpA/B to P(3HB) granules. IbpA/B seemed to play an important role in recombinant E. coli producing P(3HB) by stabilizing the interface between the hydrophobic P(3HB) granules and the hydrophilic cytoplasm. Thus, IbpA/B were found to act like phasins in recombinant E. coli, as they are the major proteins bound to the P(3HB) granules, affect the morphology of the granules, and reduce the amount of cytosolic proteins bound to the P(3HB) granules.

• The Transactions of the Korea Information Processing Society
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• v.6 no.6
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• pp.1581-1589
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• 1999

In B-ISDN(Broadband Integrated Services Digital Networks), the efficient implementation of multicast function is very important since the demand for distributed type of service such as VOD(Video On Demand) system is expected to grow. In this paper, the multicast performance characteristics of fully-interconnected switch fabric used for our research is the proper architecture for a small-sized switch element, and it uses bit addressing method for addressing scheme and thus it is easy to implement multicast function without adding a function block. To incorporate the bursty nature of traffic in ATM networks, we used IBP(Interrupted Bernoulli Process) model as an input traffic model. We presented and analyzed the simulation results in terms of the multicast operation of the switch. Based. on this study, it its analyzed that congestion avoidance may be feasible if we use a proper traffic control scheme by finding an overload point due to multicast.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.19 no.6
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• pp.1085-1094
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• 1994

We propose a traffic flow control scheme of B-NT with temporary cell buffering and selective cell discarding to prevent congestion state of the network nodes in B-ISDN systems to reduce or suppress output cell strams towards T interface. We define the states of the network nodes as normal, pre-congestion, and congestion. In a pre-congestion state, the loss-sensitive traffic is temporarily buffered to slow down the rate of the output traffic streams. In a congestion state, the delay-sensitive traffic is selectively discarded to suppress the output traffic streams as possible in addition to the cell buffering. We model the input cell streams and the states of the network nodes with Interrupted Bernoulli Process and 3-state Markov chain to analyze the performance of the proposed scheme in the B-NT system. The appropriate size of the cell buffer is explored by means of simulation and the influence on the performance of the proposed scheme by the network node state is discussed. As results, more than 2,00 cells of buffer size is needed for the control of medium of lower than the medium, degree of congestion occurrence in the network node while the control of high degree of congestion occurrence is nearly impossible.

• Journal of Plant Biotechnology
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• v.39 no.1
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• pp.33-48
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• 2012

As a scientific curiosity to understand the structure and the function of crops and experimental efforts to apply it to plant breeding, genetic maps have been constructed in various crops. Especially, in the case of Brassica crop, genetic mapping has been accelerated since genetic information of model plant $Arabidopsis$ was available. As a result, the whole $B.$ $rapa$ genome (A genome) sequencing has recently been done. The genome sequences offer opportunities to develop molecular markers for genetic analysis in $Brassica$ crops. RFLP markers are widely used as the basis for genetic map construction, but detection system is inefficiency. The technical efficiency and analysis speed of the PCR-based markers become more preferable for many form of $Brassica$ genome study. The massive sequence informative markers such as SSR, SNP and InDels are also available to increase the density of markers for high-resolution genetic analysis. The high density maps are invaluable resources for QTLs analysis, marker assisted selection (MAS), map-based cloning and comparative analysis within $Brassica$ as well as related crop species. Additionally, the advents of new technology, next-generation technique, have served as a momentum for molecular breeding. Here we summarize genetic and genomic resources and suggest their applications for the molecular breeding in $Brassica$ crop.