• Korean Journal of Veterinary Service
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• v.17 no.1
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• pp.1-8
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• 1994

To investigate serum neutralizing antibodies against Bovine ephemeral fever (BEF) virus, Akabane virus and Ibaraki virus in southern area of Chungnam province, Holstein sera were collected from April-May(269 heads /37 farms) and October-November (226 heads /35 farms), 1993. The results were summarized as follows ; 1. Bovine ephemeral fever.-antibody positive rates to BEF virus were 46.1% (124 heads /269 Holstein) in April-May and 53.9%(122 heads /226 Holstein) in October-November. 2. Akabane disease.-antibody positive rates to Akabne virus were 34.2％(92 heads /269 holstein) in April-May and 51.3%(116 heads /226 Holstein) in October-Novermber. 3. Ibaraki disease.-antibody positive rates to Ibaraki virus were 57.6%(155 heads /269 Holstein) in April -May and 38.5%(87 heads /226 Holstein) in October-November.

• Korean Journal of Veterinary Research
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• v.29 no.3
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• pp.343-359
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• 1989

To study the effects of ibaraki virus on preimplantation mouse embryos collected from prepubertal ICR and BALB/cByJ mice (30~40days old) by superovulation, zona pellucidaintact(ZPI) or free(ZPF) embryos(n=774) of 4- to 8-cell and morulae were exposed to $10^{5.8}$ $TCID_{50}$ of the virus up to 96 hours. The embryos were examined morphologically by observing the degeneration and hatching rates, and virologically and immunologically by determining the presence of infection with the virus, in addition, the effect of washing the embryos to remove virus possibly attached to was also investigated. The ZPI 4- to 8-cell embryos and morulae exposed to the virus showed considerably higher degeneration rate than those not exposed, for 96, and for 72 to 96 hours, respectively(p<0.01). The ZPF 4- to 8-cell embryos and morulae exposed to the virus showed considerably higher degeneration rates than those not exposed, throughout the whole culture hours in vitro (p<0.01). The ZPI 4- to 8-cell embryos and morulae not exposed to the virus showed considerably higher rates of hatched blastocyst than those exposed (p<0.01). The virus infection rates of the ZPF 4- to 8-cell embryos and morulae were significantly higher than those of the ZPI embryos according to cell culture system. The viral antigen was detected exclusively on the zona pellucida of ZPI embryos, while the antigen was evenly distributed in the blastomeres of ZPF embryos by the immunofluorescent assay. In the ZPI embryos exposed to ibaraki virus, the virus was detected in the two times-washing groups, but not in the ten times-washing groups. The results indicated that zona pellucida of murine embryos would provide an effective protection and that ten times-washing of the ZPI embryos previously exposed to the virus was effective to remove virus from the embryos.

• Korean Journal of Veterinary Service
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• v.19 no.3
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• pp.207-211
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• 1996

To investigate the serum neutralizing antibodies against Ibaraki disease virus in the western area of Kyunggi province, a sero-epidemiological survey was done from August 1995 to March 1996. The results obtained are summarized as follows : 1. An overall prevalence of the neutralizing antibodies agaist Ibaraki virus was as high as 68.6% (218 positive reactors amomg 318 heads of dairy cattle). 2. It showed the regional differences with 60.5${\ulcorner}$(46/76) in Koyang, 75.2% (100/133) in Paju and 66.1% (72/109) in Kimpo. 3. It also appeared with a seasonal difference showing 74.4% of prevalence with the mean titer higher than 60 during the mosquito season (from August to November) and 58.6% of prevalence with the mean titer 22 after the mosquito season to March. 4. Any cross reactions between Ibaraki and bluetongue viruses were not detected in the ELISA and AGID tests.

• Korean Journal of Veterinary Research
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• v.38 no.1
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• pp.167-172
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• 1998

한국 및 일본을 포함한 동아시아 지역에 벡터 매개성 바이러스에 의한 소 질병으로 인하여 경제적 손실이 몇년을 주기로 극심하게 나타나고 있다. 그러나 본 질병의 정확한 역학에 대하여는 아직까지 확실한 연구가 되어 있지 않은 상태이다. 본 연구에서는 벡터 매개성 질병에 대한 역학을 좀더 명확하게 밝히기 위하여, 일본 열도중 가장 멀리 떨어져 있으며, 아열대 지역에 속하는 오끼나와 섬에서 사육되고 있으면서 백신접종을 받지 않은 소(Japanese black cattle)를 대상으로 하여 1988년도부터 1992년도에 걸쳐 벡터 매개성 바이러스군에 대한 중화항체가를 측정하고 분석하였다. 소의 유행성 바이러스(bovine ephemeral virus)에 대한 항체 양성율은 연도 및 계절별로 크게 변화를 보였고, Ibaraki virus에 대한 항체양성율은 그 어느 다른 계절보다도 5월에 높은 항체가를 보였으며, Akabane virus에 대한 항체 양성율은 이바라키 바이러스에 대한 항체 양성율과 유사한 수준으로 나타났다. 한편 Chuzan virus에 대한 항체양성율은 계절적인 변화가 심하지 않은 것으로 나타났다.

• Journal of Veterinary Clinics
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• v.20 no.3
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• pp.423-426
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• 2003

Congenital anomalies occur at low levels in Korean antive cattle. PCR and Serum neutrlizing test against BVD, Akabane virus, Ibaraki virus, Kasba virus were examined. The results were all negative. Gross anatomical analysis was carried aout. Congenital abnormal newborn calf had 2 atropic left hindlimbs, which was nor moved autonomosly. The length of 2 atropic hindlimbs was 72 em and 48cm respectively. Both of them were curved severely at joints. Affected limbs were developed in connective tissue but lack of muscular tissue. In the abdominal region right kidney was normal. But there were two left kidneys which was half size compared with normal kidney. One of them had polycysts. In skeletal region, affected left limb is defective of head of femur and tibia.

• Journal of Veterinary Clinics
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• v.20 no.3
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• pp.419-422
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• 2003

Humeral hemimelia is a rare congenital anomaly characterized by deficiency of the humerus with relatively intact scapular. The holstein calf was healthy expecting the deformity of forelimb humerus. The examination of PCR and serum neutralizing test was carried about BVD, akabane virus, Ibaraki virus and Kasba virus in cattle. The results were all negative. Affected calf showed forelimb deformation. Other abnormalitis was not observed in brain, spinal cord, liver, kidney and other visceral organs. Amelia are very rare limb anomalies. We report on a Holstein calf with forelimb deficiencies. This case appears humeral anomalies with ulnar and radius aplasia in calf. The condition in these calves was considered the result of a recurrence of a genetic mutation affecting a putative hemimelia locus.

• Korean Journal of Veterinary Research
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• v.35 no.3
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• pp.615-623
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• 1995

Sera from 304 Holsteins or Korean native cattle were collected from slaughterhouse in Kwangju area to study the infection of major virus-borne diseases. Serum antibody titers against infectious bovine rhinotracheitis virus(IBRV), bovine viral diarrhea virus(BVDV), parainfluenza type-3 virus(PI-3V), bovine ephemeral fever virus(BEFV), bovine Ibaraki virus(BIV), bovine Akabane virus(BAKV), bovine rotavirus(BRV), bovine coronavirus(BCV) were measured by serum neutralization tests. Results which obtained were as follows. Sera from 280 cattle(92.1%) contained antibodies against BRV which rate was the highest among the 8 viruses, and serum antibodies against BCV, BVDV, BIV, BAKV, BEFV, IBRV and PI-3V were detected from 266(87.5%), 149(49%), 108(35.5%), 94(30.9%), 80(26.3%), 32(10.5%) and 24(7.9%) cattle, respectively. Prevalence of seropositives to BVDV, BIV, BAKV, BEFV were higher among Holsteins than among the Korean native cattle(P<0.05). Prevalence of antibody titers against BVDV, BIV and BEFV in Korean native cattle were higher among females than males, while more males contained antibodies to BAKV, IBRV and PI-3V than females in their blood(P<0.05). Seropositives to BVDV, BIV, BAKV, BEFV and IBRV in Holsteins were higher among females than males(P<0.05). In Korean native cattle, serum antibody titers against IBRV and PI-3V ranged from 1:2~1:32 and 1:2~1:64, respectively, while serum antibody titers against the rest 6 viruses ranged from $1:2{\sim}1:{\geq}256$. In Holsteins, serum antibody titers against IBRV and PI-3V ranged from 1:2~1:64 and 1:2~1:32, respectively, while serum antibody titers against the rest 6 viruses ranged from $1:2{\sim}1:{\geq}256$.

• Korean Journal of Veterinary Research
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• v.23 no.1
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• pp.81-89
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• 1983

Epizootics and sporadic outbreaks of the cattle disease characterized by fever, stomatitis and dysphagia were observed in Korea in September through November, 1982. The number of cattle showed typical symptoms of the disease was estimated to 73 and these animals were concentrated in Gyongido province, the central district of Korea. In other districts around Gyongido province the disease tended to be sporadic occurrence. The main lesions of the disease were defined as edema, hemorrhage, degeneration and necrosis of the oral mucosa and musculatures of the esophagus and laryngopharyngeal area, and abomasum. Of these lesions hyaline degeneration and hemorrhage of the striated muscle of the esophagus and laryngopharyngeal area were the most striking changes of the disease and rated to be the lesion of diagnostic significance. The serum samples of the cattle with typical symptoms showed significant positive titer of the antibodies against Ibaraki virus. On these clinical, epidemiological, pathological and serological findings of the disease it was diagnosed as Ibaraki disease presenting the only report on the epizootics in the countries other than Japan.

• Korean journal of applied entomology
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• v.28 no.1
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• pp.10-15
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• 1989

This studies were carried out to selected high pathogenic nuclear polyhedrosis viruses(NPVs) against Pseudaletia(=Leucania) separata for the introduction of microbiol control of the insect NPV in Korea. Among 21 NPVs, Sesamia inferens and 4 P. separata NPV strains were highly pathogenic against P. separata when fed orchard grass leaves smeared virus suspension on the 2nd instar larvae. Three NPV strains (LsNPV-F, LsNPV-G, LsNPV-Y) were more susceptible to the younger instar than the older instar P. separata larvae when fed artificial diet mixed with the virus to the insect.

• Korean Journal Plant Pathology
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• v.14 no.1
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• pp.68-73
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• 1998

Barley mild mosaic virus (BaMMV-Kor) was isolated from the southern part of Korea, and by mechanical inoculation onto barley cultivars, purification and production of antibody. BaMMV-Kor purified form infected plants were filamentous particle, with 13 nm in diameter and 250∼300 nm and 500∼650 nm in length. Antibody of BaMMV-Kor was made by injecting the purified virus to the muscle of a rabbit. In gel-diffusion test, antibody to BaMMV-Kor created spur with BaMMV-Kal and BaMMV-M, but did not make spur with BaMMV-Kor infected New Golden, Ishukushirazu, Joshushiro Hadaka and Misato Golden, but did not infect Kashimamugi, Chikurin Ibaraki 1 and Mokusekko 3. In Korean barley cultivars, BaMMV-Kor infected most of the covered barley cultivars, but did not infect Saeolbori. It also infected naked barley cultivars except Chalbori and Hinssalbori. And all the beer barley cultivars were infected by BaMMV-Kor. BaMMV-Kor had two RNAs, RNA 1 (7.5 Kb) and RNA 2 (3.5 Kb), and coat protein (33 KDa).