• Title/Summary/Keyword: ITS1-ITS4

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New Alternative Splicing Isoform and Identification of the Kinase Activity of N-Terminal Kinase-Like Protein (NTKL)

  • Merlin, Jayalal L.P.
    • Journal of Integrative Natural Science
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    • v.6 no.4
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    • pp.234-243
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    • 2013
  • N-terminal kinase-like (NTKL) protein was initially identified as a protein binding to protein kinase B (PKB, also known as Akt). Though NTKL-BP1 (NTKL-binding protein 1) has been identified as an NTKL binding protein, its functions related to binding have not yet been elucidated. Here, a new alternative spliced variant of NTKL and its association with integrin ${\beta}1$ is described, in addition to the kinase activity of NTKL and its substrate candidates. Although the phosphorylation of the candidates must be further confirmed using other experimental methods, the observation that NTKL can phosphorylate ROCK1, DYRK3, and MST1 indicates that NTKL may act as a signaling protein to regulate actin assembly, cell migration, cell growth, and to facilitate differentiation and development in an integrin-associated manner.

Synthesis of a small molecular cage consisting of three aminomethyl pyrroles and its selective fluoride recognition

  • Nam Jung, Heo;Hye Jin, Han;Jaewon, Choi;Sung Kuk, Kim
    • Journal of the Korean Magnetic Resonance Society
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    • v.26 no.4
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    • pp.40-45
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    • 2022
  • A small cage-like molecule (2) composed of three aminomethyl pyrroles and two hexa-substituted benzenes has been prepared by reduction of its iminopyrrole analogue (1) using NaBH4. It was revealed by 1H NMR spectroscopic analyses that cage molecule 2 strongly binds the fluoride anion in polar DMSO-d6 relative to CDCl3. Compared to that of compound 1, the lowered affinity of 2 for the fluoride anion is attributable to its increased electron density resulting from the production of thesecondary amine groups.

A Study on the Ritual Dress of Korean new Religions (한국 신종교 의례복식 고찰 (I))

  • 임상임
    • Journal of the Korean Society of Costume
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    • v.19
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    • pp.83-103
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    • 1992
  • This thesis researched the ritual dress of Korean new religions such as Suungyo, Mirubulgyo, Taejonggyo and WonBuddhism, According to kind, color, cloth and shape this thesis analyzed the ritual dress of them on the basis of its literatures. I. The results of the ritual dress of them through its literatures are as follows: 1. In case of Suungyo, there are Songwansonbok, Togwandobok, Pulmogwan, Ch'onui, Chap'ae , Yukhwangang, Pobdae, Hakp'yo and Kyonjang in its kind. In its color there are Huk, Cha, Hoe, Chahwang, Hong, Paek, Hwang, ROk, and Nam. Chemical fiber with one layer is used in its cloth. Its shape is made by a comprise between the feature of Korean dress, chuui and that of the existing dress of Confucianim, Buddhism and Taoism. 2. In case of Mirukbulgyo, Inhwag-wan, Chongbok, P'oui, Tae, Moson, Yomju and tanju are systematized in its kind. In its color there are Paek, Huk, ch'ong,Chok, Ok and Rok, Kongdan and chemical fiber with one layer are used in its cloth. Its shape has a symbolic form of Yu'Pul and son based upon Korean dress, Chuui. In its symbol Inhwagwan symbolized Confucianism and Chongbok Taoism and P'oui Buddhism. 3. In case of Taejonggyo, there are Chusabok, Sjhobok, Yewonbok, Chusamo, Yewonmo, Tae and Hwa in its kind. In its color there are Paek(main one), Nam, Huk, Chaju, Chok, Hwang and Hoe. Kongdan with both sides is elaborately used in its cloth. Its shape thken from Korean dress means the succession of the national spirit of the white-clad folk. 4. In case of won Buddhism, there are kybok and Pobrak in its kind. In its color there are Paek, Huk and Hoe---especially, both Hwangt'o and Hwang are used in Pobrak, Anyone can use cloth at his will. In its shape Kyoblk for man is Yangbok and Kyobok for woman is Tongch'ima and Chogori, a style of Korean dress. Pobrak is a form Tanryong worm with Rakja.

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Effect of Sulfate on the Reaction of 3CaO.SiO2 Formation in Presence of Clinker Melt and Its Appearance in Clinker ($SO_3$가 클링커 액상이 존재하는 $3CaO.SiO_2$ 생성반응에 미치는 영향과 클링커안에서의 존재상태)

  • 정해문;한기성;최상흘
    • Journal of the Korean Ceramic Society
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    • v.30 no.1
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    • pp.7-16
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    • 1993
  • Effect of sulfate on the reactionof C3S formation in presence of clinker melt and its appearance in clinker were investigated, using (NH4)2SO4 and CaSO4 as sulfate sources. When (NH4)2SO4 and CaSO4 were added, both showed the similar results, 1.0wt% of sulfate could promoted the reaction of C3S formation, however for its content of more than 2.0wt%, the formation of C3S was prevented. Residual limit of sulfate to C3S formation is about 1.4wt%. Appearances of sulfate were C4A3l and CaSO4 in interstitial phase. For the addition of (NH4)2SO4 or CaSO4 of 20wt%~4.0wt%, C3S grains showed the hypertrophic growth. We might consider that, because sulfate reduced the surface tension and viscosity of the clinker melt, C3S crystals were precipitated below 140$0^{\circ}C$, and the crystlas of C3S were coalesced and linked in the same crystallographical direction with increasing temperature becuase of their rapid growth rate.

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A Case Study of a Resource Reservation Protocol in IP Based Wireless Access Networks for ITS Service

  • Jung Kwang Mo;Jung Hyun Chul;Min Sang Won
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.4 no.1 s.6
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    • pp.107-115
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    • 2005
  • For effective IP based service implementation on the wireless network environments, wireless network including the ITS network have to support QoS guaranteed protocol such as a RSVP. RSVP is a resource reservation protocol for Internet environment, and its scalability makes easy to implement RSVP over the various IP transport technologies. But for the If based ITS wireless network environment, RSVP is not suitable, since by its path setup procedure characteristic. In the wireless access network for ITS service, when a mobile node moves to other domain it must perform registration procedure. But the registration procedure is time consuming steps, so if a RSVP session was already established in the previous domain, the RSYP session may be disconnected and the time to re establish a new RSVP session is long enough to cause serious packet loss. In this paper, we propose a pre-path reservation mechanism for applying the RSVP in wireless access networks for ITS. In the pre-path reservation mechanism, the resource reservation procedure occurred during a mobile node's handoff time. An access point in wireless access network performs this procedure when the mobile node attempts handoff The access point executes pre-path reservation procedure as a proxy, since the mobile node does not have IP address until the address allocation procedure is finished in a new domain.

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Property Analyses of Deposits and Landform in Tidal Flat using Satellite Image

  • Jo, Myung-Hee;Sugimori, Yasuhiro;Jo, Wha-Ryong
    • Proceedings of the KSRS Conference
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    • 1998.09a
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    • pp.110-115
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    • 1998
  • Through the ISODATA method, the micro-landform of Julpo-Bay tidal flat was classified into mudflat, mixedflat, and sandflat using Landsat TM image. Each showed an apparent differences in its topographical characteristics and grain size composition. For example, mudflats are formed with flat faces and tidal channel of dissected gully. Its characteristics of grain size analysis that the grains have less than mean grain size 4 phi. Its sorting is bad (higher than 1 S.D.), and it showed strongly positive skewness. But sandflat is topographically flat without tidal channel. It has developed with ripple marks. According to the grain size analysis of deposits, the soil is coarse size with 90% of sand and its sorting is well(lower than 1 S.D.) Also, it showed strongly negative skewness. Mixed flat is in between mudflat and sandflat in its characteristics.

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Impact of Early Menopause on the Risk of Metabolic Syndrome and Its Components in Korean Women: Using the 4th to 8th Korea National Health and Nutrition Examination Survey (2007~2021) (한국 여성의 조기 폐경이 대사증후군과 구성요소 유병 위험에 미치는 영향: 국민건강영양조사 제4~8기(2007~2021) 자료를 이용하여)

  • So, Eun Sun
    • Journal of East-West Nursing Research
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    • v.30 no.1
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    • pp.60-69
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    • 2024
  • Purpose: This study was conducted to determine the impact of premature menopause (PM) on metabolic syndrome and its components to determine its contribution to the health of Korean women undergoing natural menopause. Methods: This study used data from the 4th to 8th National Health and Nutrition Examination Survey (2007~2021). A total of 11,989 women aged 30 to 65 who responded to questions about their current menstrual status, natural menopause status and its age. Complex sample multivariate Cox proportional hazards regression analysis was used. Results: Compared with women with late menopause, women with PM had a 1.34 times higher risk of developing metabolic syndrome. Excluding the risk of high blood pressure, women with PM had a significantly higher risks of increased waist circumference, decreased high-density lipoprotein, increased risk of high triglycerides, and increased fasting blood glucose. Conclusions: The results of this study provided data that can be applied to policies or interventions to prevent or manage metabolic syndrome in women with PM in Korea.

A Study on Flow Characteristics of Lid-Driven Square Cavity (정방형 벨트구동 캐비티의 흐름특성에 관한 연구)

  • 김진구;조대환;김춘식;이영호
    • Journal of Advanced Marine Engineering and Technology
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    • v.21 no.4
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    • pp.430-436
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    • 1997
  • Time-dependent Flow characteristics of a two-dimensional lid-driven square cavity flow of six high Reynolds numbers, $10^4 2{\times}lO^4., 3{\times}l0^4, 5{\times}lO^4, 7.5{\times}lO^4$ and $10^5$ were investigated. A convection conservative difference scheme based upon SOLA to maintain the nearly 2nd-order spatial accuracy was adopted on irregular grid formation. Irregular grid number is $80{\times}80$ and its minimum size is about 1/400 of the cavity height(H) and its maximum is about 1/53 H. The result shows that at Re= $10^4$, periodic migration of small eddies appearing in corner separation region and its temporal sinusoidal fluctuation are represented. And another critical Reynolds number which shows the commencement of flow randomness emerging from the periodic fluctuation is assumed to be around Re= $1.5{\times}10$. At five higher Reynolds numbers ($2{\times}lO^4., 3{\times}l0^4, 5{\times}lO^4, 7.5{\times}lO^4$ and $10^5$), an organizing structure of four consecutive vortices similar to a Moffat vortex at two lower corners is revealed from time-mean flow patterns.

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Vegetative Growth and Phylogenetic Relationship of Commercially Cultivated Strains of Pleurotus eryngii based on ITS sequence and RAPD

  • Alam, Nuhu;Shim, Mi-Ja;Lee, Min-Woong;Shin, Pyung-Gyun;Yoo, Young-Bok;Lee, Tae-Soo
    • Mycobiology
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    • v.37 no.4
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    • pp.258-266
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    • 2009
  • Pleurotus eryngii, known as king oyster mushroom has been widely used for nutritional and medicinal purposes. This study was initiated to screen the suitable conditions for mycelial growth and to determine the phylogenetic relationship of the selected strains. Optimal mycelial growth was observed at $30{^{\circ}C}$ and minimum mycelial growth observed at $10{^{\circ}C}$. This mushroom tolerates a broad pH range for mycelial growth, with most favorable growth observed at pH 6. Results also indicated that glucose peptone, yeast malt extract and mushroom complete media were favorable growth media, while Hennerberg and Hoppkins media were unfavorable. Dextrin was the best and xylose the least effective carbon sources. Results revealed that inorganic nitrogen sources were less effective than organic sources for the mycelial growth of P. eryngii. Investigation of genetic diversity is necessary to identify the strains. The ITS region of rDNA were amplified using PCR. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 214 to 222 bp and 145 to 236 bp, respectively. The sequence of ITS2 was more variable than that of ITS1, and the 5.8S sequences were identical. A phylogenetic tree based on the ITS region sequences indicated that selected strains could be classified into six clusters. Fourteen IUM and ATCC- 90212 strains were also analyzed by RAPD with 20 arbitrary primers. Fourteen of these primers were efficiently amplified the genomic DNA. The number of amplified bands varied with the primers and strains, with polymorphic fragments in the range from 0.2 to 2.3 kb.

Development of Molecular Markers for the authentication of Zanthoxyli Pericarpium by the analysis of rDNA-ITS DNA barcode regions (rDNA-ITS DNA 바코드 부위 분석을 통한 산초(山椒) 기원종 감별용 유전자 마커 개발)

  • Kim, Wook Jin;Ji, Yunui;Lee, Young Mi;Kang, Young Min;Choi, Goya;Moon, Byeong Cheol
    • The Korea Journal of Herbology
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    • v.30 no.3
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    • pp.41-47
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    • 2015
  • Objectives : Due to the morphological similarity of the pericarp and description of multi-species in National Pharmacopoeia of Korea and China, the Zanthoxylum Pericarpium is difficult to authenticate adulterant in species levels. Therefore, we introduced the sequence analysis of DNA barcode and identification of single nucleotide polymorphism(SNP) to establish a reliable tool for the distinction of Zanthoxylum Pericarpium from its adulterants. Methods : To analyze DNA barcode region, genomic DNA was extracted from twenty-four specimens of authentic Zanthoxylum species and inauthentic adulterant and the individual internal transcribed spacer regions (rDNA-ITS and ITS2) of nuclear ribosomal RNA gene were amplified using ITS1, ITS2-S2F, and ITS4 primer. For identification of species-specific sequences, a comparative analysis was performed using entire DNA barcode sequences. Results : In comparison of four Zanthoxylum ITS2 sequences, we identified 16, 4, 6, and 4 distinct species-specific nucleotides enough to distinguish Z. schinifolium, Z. bungeanum, Z. piperitum, and Z. simulans, respectively. The sequence differences were available genetic marker to discriminate four species. Futhermore, phylogenetic relationship revealed a clear classification between different Zanthoxylum species showing 4 different clusters. These results indicated that comparative analysis of ITS2 DNA barcode was an useful genetic marker to authenticate Zanthoxylum Pericarpium in species levels. Conclusions : The marker nucleotides, enough to distinguish Z. schinifolium, Z. piperitum, Z. bungeanum, and Z. simulans, were obtained at 30 SNP marker nucleotides from ITS2 sequences. These differences could be used to authenticate official Zanthoxylum Pericarpium from its adulterants as well as discriminating each four species.