Browse > Article
http://dx.doi.org/10.4489/MYCO.2009.37.4.258

Vegetative Growth and Phylogenetic Relationship of Commercially Cultivated Strains of Pleurotus eryngii based on ITS sequence and RAPD  

Alam, Nuhu (Department of Biology, University of Incheon)
Shim, Mi-Ja (Department of Life Science, University of Seoul)
Lee, Min-Woong (Department of Biology, Dongguk University)
Shin, Pyung-Gyun (Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horticulture & Herbal Science, RDA)
Yoo, Young-Bok (Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horticulture & Herbal Science, RDA)
Lee, Tae-Soo (Department of Biology, University of Incheon)
Publication Information
Mycobiology / v.37, no.4, 2009 , pp. 258-266 More about this Journal
Abstract
Pleurotus eryngii, known as king oyster mushroom has been widely used for nutritional and medicinal purposes. This study was initiated to screen the suitable conditions for mycelial growth and to determine the phylogenetic relationship of the selected strains. Optimal mycelial growth was observed at $30{^{\circ}C}$ and minimum mycelial growth observed at $10{^{\circ}C}$. This mushroom tolerates a broad pH range for mycelial growth, with most favorable growth observed at pH 6. Results also indicated that glucose peptone, yeast malt extract and mushroom complete media were favorable growth media, while Hennerberg and Hoppkins media were unfavorable. Dextrin was the best and xylose the least effective carbon sources. Results revealed that inorganic nitrogen sources were less effective than organic sources for the mycelial growth of P. eryngii. Investigation of genetic diversity is necessary to identify the strains. The ITS region of rDNA were amplified using PCR. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 214 to 222 bp and 145 to 236 bp, respectively. The sequence of ITS2 was more variable than that of ITS1, and the 5.8S sequences were identical. A phylogenetic tree based on the ITS region sequences indicated that selected strains could be classified into six clusters. Fourteen IUM and ATCC- 90212 strains were also analyzed by RAPD with 20 arbitrary primers. Fourteen of these primers were efficiently amplified the genomic DNA. The number of amplified bands varied with the primers and strains, with polymorphic fragments in the range from 0.2 to 2.3 kb.
Keywords
ITS; Phylogenetic relation; Pleurotus eryngii; RAPD; Vegetative growth;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Mayer, A. M. and Staples, R. C. 2002. Laccase: new functions for an old enzyme. Phytochemistry 60:551-565   DOI   ScienceOn
2 Nei, M. and Li, W. H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76:5269-5273   DOI   ScienceOn
3 Ro, H. S., Kim, S. S., Ryu, J. S., Jeon, C. O., Lee, T. S. and Lee, H. S. 2007. Comparative studies on the diversity of the edible mushroom Pleurotus eryngii: ITS sequence analysis, RAPD fingerprinting and physiological characteristics. Myco. Res. 111:710-715   DOI   ScienceOn
4 Soden, D. M. and Dobson, A. D. W. 2001. Differential regulation of laccase gene expression in Pleurotus sajor-caju. Microbiology 147:1755-1763   DOI
5 Sung, J. M., Kim, C. H., Yang, K. J., Lee, H. K. and Kim, Y. S. 1993. Studies on distribution and utilization of Cordyceps militaris and C. nutans. Kor. J. Mycol. 21:94-105   과학기술학회마을
6 Zervakis, G. I., Venturella, G. and Papadopoulou, K. 2001. Genetic polymorphism and taxonomic infrastructure of the Pleurotus eryngii species-complex as determined by RAPD analysis, isozyme profiles and ecomorphological characters. Microbiology 147:3183-3194   DOI   ScienceOn
7 Lewinsohn, D., Nevo, E., Wasser, S. P., Hadar, Y. and Beharav, A. 2001. Genetic diversity in populations of the Pleurotus eryngii complex in Israel. Myco. Res. 105:941-951   DOI   ScienceOn
8 Lee, S. B. and Taylor, J. W. 1990. Isolation of DNA from fungal mycelia and single spores. In: PCR protocol: A Guide to Methods and Applications, pp. 282-287. Eds. M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White. Academic Press, San Diego
9 Alam, N., Jaysinghe, C., Cha, Y. J., Kim, J. H. and Lee, T. S. 2008. Screening of suitable conditions for mycelial growth of wild strains of Pholiota adiposa. Bull. Life & Environ. Sci. 2:105-112
10 Cubero, O. F., Crespo, A., Fatehi, J. and Bridge, P. D. 1999. DNA extraction and PCR amplification method suitable for fresh, herbarium stored, lichenized and other fungi. Pant Syst. Evol. 216:243-249   DOI   ScienceOn
11 Tuckwell, D. S., Nicholson, M. J., Mc-Sweeney, C. S., Theodorou, M. K. and Brookman, J. L. 2005. The rapid assignment of ruminal fungi to presumptive genera using ITS1 and ITS2 RNA secondary structures to produce group specific fingerprints. Microbiology 151:1557-1567   DOI   ScienceOn
12 Watling, R. 1992. Observations on the Bolbitiaceae - 30. Some Brazilian taxa. Boletín de la Sociedad Argentina de Botánica. 28:77-103
13 Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol. Biol. Evol. 4:406-425
14 White, T. J., Bruns, T., Lee, S. and Taylor, J. W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocol: A Guide to Methods and Applications, pp. 315-322. Eds. M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White. Academic Press, SanDiego
15 Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A. and Tingey, S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531-6535   DOI   ScienceOn
16 Alam, N., Shim, M. J., Lee, M. W., Shin, P. G., Yoo, Y. B. and Lee, T. S. 2009. Phylogenetic relationship in different commercial strains of Pleurotus nebrodensis based on ITS sequence and RAPD. Mycobiology 37:183-188   과학기술학회마을   DOI   ScienceOn
17 Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighing position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680   DOI   ScienceOn
18 Choi, D., Kang, S. H., Song, Y. H., Kwun, K. H., Jeong, K. J. and Cha, W. S. 2005. Exopolysaccharide production in liquid culture of Pleurotus ferulae. J. Microbiol. Biotecnol. 15:368-375
19 Felsenstein, J. 1985. Confidence limit on phylogenies: an approach using the bootstrap. Evolution. 39:783-791   DOI   ScienceOn
20 Hur, H. 2008. Cultural characteristics and log-mediated cultivation of the medicinal mushroom, Phellinus linteus. Mycobiology 36:81-87   과학기술학회마을   DOI   ScienceOn
21 Shim, S. M., Oh, Y. H., Lee, K. R., Kim, S. H., Im, K. H., Kim, J. W., Lee, U. Y., Shim, J. O., Shim, M. J., Lee, M. W., Ro, H. S., Lee, H. S. and Lee, T. S. 2005. The characteristics of cultural conditions for the mycelial growth of Macrolepiota procera. Mycobiology 33:15-18   과학기술학회마을   DOI   ScienceOn
22 Shim, J. O., Son, S. G., Kim, Y. H., Lee, Y. S., Lee, J. Y., Lee, T. S., Lee, S. S. and Lee, M. W. 1997. The cultural conditions affecting the mycelial growth of Grifola umbellata. Kor. J. Mycol. 25:209-218   과학기술학회마을