• Title/Summary/Keyword: ITS1 sequencing

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S&T Theories and National Competitive Advantages in South Korea

  • Kim, Sang-Tae
    • World Technopolis Review
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    • v.1 no.3
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    • pp.186-191
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    • 2012
  • To understand South Korea's economic success several contending paradigms have emerged, ranging from a neo-classical explanation to a statist perspective, and Schumpeterian structuralism. The Korean miracle can be seen as a product of multiple factors involving the strategic choice of export-led growth, timely and active human-capital investment, business activism, and individual innovative ideas. However, South Korea needs more complex and innovative ideas to continue its both economic growth and S&T Development. The ideas of Schumpeterian structuralist needs to be more emphasized. Changing state-society relations and subsequent policy choice are greatly influenced by the industrial life cycle. The industrial life cycle is closely related to the historical sequencing and diffusion of technology. The complex mixture of statist-market-Schumpeterian structuralist perspectives could lead more technologically advanced society in South Korea.

Biological Phosphorus Removal using the Sequencing Batch Reactor Process (연속회분식반응조를 이용한 생물학적인 인 제거 연구)

  • Yang, Hyung-Jae;Shin, Eung-Bai;Chung, Yun-Chul;Choi, Hun-Geun
    • Journal of Korean Society on Water Environment
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    • v.16 no.4
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    • pp.533-539
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    • 2000
  • A bench-scale reactor using SBR process was experimented with an synthetic wastewater. The main purpose of this investigation was to evaluate applicability in the field and process removal efficiencies in terms of BOD and T-P and its corresponding kinetic parameters. Removal rate of phosphorus was 77% in terms of total phosphorus. Effluent concentrations were $9.8mg/{\ell}$ BOD and $1.1mg/{\ell}$ T-P. Effluent quality was maintained consistently stable by controlling decant volume and operating cycles. The efficiency for phosphorus removal was increased due to decrease in BOD-SS loading value in the range of $0.25{\leq}$aeration time ratio${\leq}0.52$.

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Isolation and Identification of Fungi for Decolorization of Synthetic Dyes

  • Lee, Jang-Hoon;Nam, Youn-Ku;Kwon, Hyuk-Ku
    • Proceedings of the Korean Environmental Health Society Conference
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    • 2005.12a
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    • pp.95-102
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    • 2005
  • For decolorization of synthetic dyes, Isolate fungi were investigated for the decolorization of 8 industrial dyes. One fungus isolated from textile wastewater collected from Banweol industrial complex, Korea showed excellent ability for removing synthetic dyes. Internal Transcribed Spacers (ITS) sequencing result was confirmed as the new Basidomycetes species. HUE05-1 The optimal decolorizaton conditions were pH5, 30$^{\circ}C$ and aerobic condition. HUE05-1 was completely decolorized all dyes in both solid and liquid condition. The result is decolorization effect at Reactive Orange 16; 97.12%, Reactive Blue 19; 92.09%, Reactive Blue 49; 97.04%, Reactive Yellow 145; 95.53%, Acid Orange 10; 99.18%, Acid Violet 43; 98.73%, Acid Blue 350; 94.71%, Disperse Blue 106; 90.07%.

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Amino Acid Sequence Homology of Hybrid Poplar O-methyltransferuse Involved in Lignin Biosynthesis

  • Park, Young-Goo;Sul, Ill-Whan;Shin, Dong-Ill;Park, Jang-Won;Park, Hee-Sung
    • Journal of Plant Biotechnology
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    • v.3 no.3
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    • pp.131-134
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    • 2001
  • In $\lambda$-Zap II vector system, a cDNA library was constructed for the developing secondary xylem mRNA from hybrid poplar, Populus nigra x maximowiczii. A cDNA clone of 1.5 kb in size, pOMTB1.4 encoding a lignin-bispecific O-methyltransferase was screened by plaque hybridization using a probe of 540 bp cDNA amplified by polymerase chain reaction from the cDNA library and identified by nucleotide sequencing. Its nucleotide sequence contains one open reading frame of 366 amino acids. The deduced amino acid sequence in comparison with that of Populus tremuloides showed the differences of 9 amino acids and revealed 85-99% homology among alfalfa, poplar and aspen.

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The Complete Nucleotide Sequence of Alkalophilic Bacillus sp. K-17 $\beta$-Xylosidase Gene

  • Chun, Hyo-Kon;Ko, Hak-Ryong;Kho, Yung-Hee
    • Journal of Microbiology and Biotechnology
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    • v.1 no.1
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    • pp.45-49
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    • 1991
  • The complete nucleotide sequence of alkalophilic Bacillus sp. K-17 $\beta$-xylosidase gene and its flanking regions were established. A 1263-bp of an open reading frame for $\beta$-xylosidase was observed. The molecular weight (50, 521 dalton), deduced from the nucleotide sequence of $\beta$-xylosidase gene, agreed with the result obtained by SDS-polyacrylamide gel electrophoresis of the purified enzyme (51, 000 dalton). The Shine-Dalgarno sequence, 5'-GAGGAGG-3', was found 8 bp upstream of the initiation codon ATG. The -10 sequence (TAAAAT) in the promoter region for $\beta$-xylosidase gene was similar to the consensus sequence for Bacillus subtilis RNA polymerase, whereas the -35 sequence (TCGATCA) different from all the known -35 regions in the promoter for Bacillus subtilis RNA polymerase.

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Characterization of the Nucleotide Sequence of a Polyubiquitin Gene (PUBC1) from Arabian Camel, Camelus dromedarius

  • Al-Khedhairy, Abdulaziz Ali A.
    • BMB Reports
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    • v.37 no.2
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    • pp.144-147
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    • 2004
  • Molecular amplification and sequencing of genomic DNA that encodes camel polyubiquitin (PUBC1) was performed by a polymerase chain reaction (PCR) using various sets of primers. The amplification generated a number of DNA fragments, which were sequenced and compared with the polyubiquitin coding sequences of various species. One DNA fragment that conformed to 325 bp was found to be 95 and 88% homologous to the sequences of human polyubiquitin B and C, respectively. The DNA translated into 108 amino acids that corresponded to two fused units of ubiquitin with no intervening sequence, which indicates that it is a polyubiquitin and contains at least two units of ubiquitin. Although, variations were found in the nucleotide sequence when compared to those of other species, the amino acid sequence was 100% homologous to the polyubiquitin sequences of humans, mice, and rats. This is the first report of the polyubiquitin DNA coding sequence and its corresponding amino acid sequence from camels, amplified using direct genomic DNA preparations.

Draft Genome Sequence of the White-Rot Fungus Schizophyllum Commune IUM1114-SS01

  • Kim, Da-Woon;Nam, Junhyeok;Nguyen, Ha Thi Kim;Lee, Jiwon;Choi, Yongjun;Choi, Jaehyuk
    • Mycobiology
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    • v.49 no.1
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    • pp.86-88
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    • 2021
  • The monokaryotic strain, Schizophyllum commune strain IUM1114-SS01, was generated from a basidiospore of dikaryotic parental strain IUM1114. It even showed the decolorizing activities for several textile dyes much better than its parental strain. Based on the results of a single-molecule real-time sequencing technology, we present the draft genome of S. commune IUM1114-SS01, comprising 41.1 Mb with GC contents of the genome were 57.44%. Among 13,380 protein-coding genes, 534 genes are carbon hydrate-active enzyme coding genes.

Morphological Characteristics and Molecular Identification of Calonectria ilicicola Causing Soybean Red Crown Rot (콩 검은뿌리썩음병균(Calonectria ilicicola)의 형태적 특징과 유전자 수준에서의 동정)

  • Park, Seong-Woo;Kim, Hong-Sik;Woo, Sun-Hee;Shim, Heong-Kwon;Kim, Heung-Tae
    • Research in Plant Disease
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    • v.12 no.3
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    • pp.178-184
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    • 2006
  • The 18 isolates of soybean red crown rot pathogen(Calonectria ilicicola) were isolated from infected soybean plants showing chlorosis symptom and red perithecia sign in 2002 and 2003 season in Korea. The morphological characteristics of the typical isolate SC03-15, such as macroconidia and macrovesicles in anamorph as well as perithecia, asci and ascospores in teleomorph, were observed from red crown rot pathogens. The perithecia were red-brown globose to ovoid, and $357.9(312.5{\sim}391.5.7){\mu}m$ high and $474.3(419{\sim}535){\mu}m$ diameter. Asci were hyaline and clavate and included 8 ascospores, which were straight to slightly curved, 1(-3)-septate, and $36.9(31.1{\sim}49.4){\times}5.2(4.5{\sim}6.1){\mu}m$. Macroconidia in anamorph were cylindrical, hyaline and in size $73.2(68.1{\sim}77.6){\times}5.8(4.9{\sim}6.8){\mu}m$. Further identification process was performed through molecular method, such as a phylogenetic analysis by rDNA sequencing of ITS and $\beta$-tubuline genes of all isolates tested in this study. All isolates were identified as Calonectria ilicicola by DNA sequencing of ITS and $\beta$-tubuline genes, and morphological characteristics.

Identification of Botrytis cinerea, the Cause of Post-Harvest Gray Mold on Broccoli in Korea

  • Aktaruzzaman, Md.;Afroz, Tania;Hong, Sae-Jin;Kim, Byung-Sup
    • Research in Plant Disease
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    • v.23 no.4
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    • pp.372-378
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    • 2017
  • In this study, we identified the causative agent of post-harvest gray mold on broccoli that was stored on a farmers' cooperative in Pyeongchang, Gangwon Province, South Korea, in September 2016. The incidence of gray mold on broccoli was 10-30% after 3-5 weeks of storage at $3^{\circ}C$. Symptoms included brownish curd and gray-to-dark mycelia with abundant conidia on the infected broccoli curds. The fungus was isolated from infected fruit and cultured on potato dextrose agar. To identify the fungus, we examined the morphological characteristics and sequenced the rDNA of the fungus and confirmed its pathogenicity according to Koch's postulates. The results of the morphological examination, pathogenicity test, and sequencing of the 5.8S rDNA of the internal transcribed spacer regions (ITS1 and ITS4) and three nuclear protein-coding genes, G3PDH, HSP60, and RPB2, revealed that the causal agent of the post-harvest gray mold on broccoli was Botrytis cinerea. To our knowledge, this is the first report of post-harvest gray mold on broccoli in Korea.