• Title/Summary/Keyword: ITS1 / 5.8S / ITS2

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Water vapor in high-mass star-forming regions and PDRs: the Herschel/HIFI view

  • Choi, Yunhee;van der Tak, Floris F.S.;van Dishoeck, Ewine F.;Bergin, Edwin A.
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.2
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    • pp.42.2-43
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    • 2015
  • Massive stars play a major role in the interstellar energy budget and the shaping of the galactic environment. The water molecule is thought to be a sensitive tracer of physical conditions and dynamics in star-forming regions because of its large abundance variations between hot and cold regions. Herschel/HIFI allows us to observe the multiple rotational transitions of H2O including the ground-state levels, and its isotopologues toward high-mass star-forming regions in different evolutionary stages. Photodissociation regions (PDRs) are also targeted to investigate the distribution of water and its chemistry. We present line profiles and maps of H2O using data from two guaranteed-time key programs "Water In Star-forming regions with Herschel" and "Herschel observations of EXtra-Ordinary Sources". We analyze the temperature and density structures using LTE and non-LTE methods. We also estimate turbulent and expansion velocities, and abundance of water in the inner and outer envelopes using the 1D radiative transfer code. Around high-mass protostars we find H2O abundances of ~10-8-10-9 for the outer envelope and ~10-4-10-5 for the inner envelope, and expansion and turbulent velocities range from 1.0 km s-1 to 2.0 km s-1. The abundances and kinematic parameters of the sources do not show clear trends with evolutionary indicators. The Herschel/HIFI mapping observations of H2O toward the Orion Bar PDR show that H2O emission peaks between the shielded dense gas and the radicals position, in agreement with the theoretical and the observational PDR structure. The derived H2O abundance is ~10-7 and peaks at the depth of AV ~8 mag from the ionization front. Together with the low ortho-to-para ratio of H2O (~1) presented by Choi et al. (2014), our results show that the chemistry of water in the Orion Bar is dominated by photodesorption and photodissociation.

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Studies on the Consumptive Use of Irrigated Water in Upland (田作物 水分消費量 調査 硏究)

  • Kim, Shi-Won;Lee, Kyong-Hi;Doh, Duk-Hyun
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.26 no.2
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    • pp.47-58
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    • 1984
  • The study results of the mosture consumption character and irrigation effect of tomato, red pepper and chinese cabbage, in case the soil moisture is kept with different moisture content by the soil properties(loam, sandy loam, sand), are summarized as follows: 1. The available rainfall under bare soil condition had an order of sand>sandy loam> loam and their average was 64.2%. 2. Total moisture consumption under bare soil condition had an order of loam>sandy loam>sand and their average was 4.2mm. 3. The amount of irrigated water to keep certain soil moisture under bare soil condition showed minimum in sand and maximum in loam. It is considered because the capillary phenomenon was more developed in loam. 4. Total moisture consumption of tomatoes under premature cultivation showed 925mm in maximum and had on order of loam>sandy loam>sand. In the aspect of re-irrigation point, it had an order of PF 1.5> PF 1.7>PF 2.1. In case the twenty years's drought frequency was taken into account, the target amount of irrigation water meeded for premature cultivation was 916mm and its average daily moisture consumption was 10.8mm. 5. Total moisture consumption of red pepper under open cultivation showed 1145mm in maximum and had an order of loam>Sandy loam>sand. In the aspect of re-irrigation frequency was taken into consideration the target amount of irrigation water was 1,174.8mm and its average daily moisture consumption was 8.0mm. 6. Total moisture consumption of autumn chinese cabbages was 349mm in maximum and had an order of loam>sandy loam>sand. In the aspect of re-irrigation point, it had an order of PF 1.5>PF 2.1>PF 2.7. In case the twenty year's drought frequency was taken into account, the target amount of irrigation water needed for chinese cabbage cultivation was 259.5mm and its average daily moisture consumption was 6.5mm. 7. It is effective to keep the soil moisture of tomato from PF 1.5 to PF 2.1 in loam and the soil moisture control was effective in sandy loam than red pepper and chinese cabbage. In sand, the production was severaly decreased and the re-irrigation point of PF 1.5 was effective.

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Taxonomic Revision of Notohymena gangwonensis (Protozoa: Ciliophora), with Notes on Its Cortical Granules and Scanning Electron Micrographs

  • Moon, Ji Hye;Kim, Kang-San;Chae, Kyu Seok;Min, Gi-Sik;Jung, Jae-Ho
    • Animal Systematics, Evolution and Diversity
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    • v.36 no.2
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    • pp.113-122
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    • 2020
  • From a moss sample, we isolated and identified Notohymena gangwonensis Kim et al., 2019 based on morphological and molecular data. The moss and type population has completely identical 18S rRNA (nuclear small subunit ribosomal RNA) gene sequences and both are highly similar in morphological and morphometric attributes, except for the diameter and arrangement of the cortical granules. Thus, we reexamined the type materials(i.e., micrographs and gDNA) and resulted in finding mistakes made by the authors of the species. Based on these data and supporting materials newly obtained (i.e., internal transcribed spacer [ITS] 1, ITS2, 5.8S, and partial 28S rDNA sequences, and scanning electron micrographs), we provide improved diagnosis of the species to clarify its identity. In addition, a key for Notohymena species is provided.

Biophysical study of bioactive-substance conformation and interaction with drugs in solution

  • Yu, Byung-Sul;Lee, Bong-Jin;Sohn, Dong-Hwan
    • Archives of Pharmacal Research
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    • v.8 no.3
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    • pp.109-117
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    • 1985
  • The interaction of salicylic acid (S. A.), salicylamide (S,M) with nucleic acid base derivatives such as 9-ethyl adenine (A), 1-cyclohexyl uracil (U), 2', 3'-benzylidine-5' trityl-cytidine (C), gaunosine-2', 3', 5'-isobutylate (G) has been spectroscopically investigated to determine the binding mechanism. NMR and IR spectra were measured in nonpolar solvents. The association constant K of the formation of complex was calculated from the IR spectra. Compounds S. A. and A form a 1:1 or 1:2 cyclic hydrogen-bonded complex depending on the sample concentration. Compounds S. A. and U form a 1:1 or 1:2 hydrogen-bonded complex on the sample concentration. Compounds S. A. and C form a 2:1 hydrogen-bonded complex at low concentration (0.0016M). Compound S. A. binds compound G, but its binding does not completely break the self-association of compound G, Compound S. M. binds compounds A. U. C. G. very weakly.

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Effect of Garlic on Quality of Low Salted Anchovy - 2. Changes of Nitrogenous Compounds - (마늘 첨가가 저염 멸치젓의 품질에 미치는 영향 - 제2보 함질소화합물의 변화 -)

  • 진양호;권오천;성낙주;신정혜;강민정
    • Culinary science and hospitality research
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    • v.8 no.3
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    • pp.279-294
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    • 2002
  • The anchovy, Engraulis japonica, were prepared with two different salt concentration of 20%, 10% which was added 2, 5, 5 and 10 % of grind garlic(LSA 1, 2, 3, 4) and garlic juice(LSB 1, 2, 3, 4), respectively. The experimental samples were taken at 30, 60, 90 and 110 days fermentation, which were analyzed VBN(volatile basic nitrogen), amino acids, nucleotides and their related compounds and amino acids. VBN increased rapidly until 110 days fermentation, its contents in samples containing of 10% salt(CB) were increased about 2.4 times as compared with those of 30 days fermentation. And then its contents of 110 days fermented samples increased at average 2.3 times more than 30 days fermented samples containing grind garlic of 2, 5, 8% and garlic juice of 2%. Nucleotide and their related compounds were detected AMP, UMP, IMP, inosine and hypoxanthine. Hypoxanthine was dominants in all samples and increased steadily during fermentation of anchovy. The contents of composition amino acids decreased gradually during it's fermentation, but those content decreased 33% in CO group, 42% in the CB group and 38%(average) in the other garlic added low salt groups.

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Validation of an Instrument to Measure Exercise of Self-Care Agency and its Predictors (자가간호역량의 행사 측정도구의 타당성 및 예측요인 조사)

  • 소향숙;이은숙
    • Journal of Korean Academy of Nursing
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    • v.19 no.3
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    • pp.273-284
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    • 1989
  • The aim of this investigation was to delineate concepts contributing to the Exercise of Self-Care Agency (E.S.C.A.) Scale developed by Kearney & Fleischer (1979) and to test its construct validity and its predictors. The results are summarized as follows ; 1. By means of principal factor analysis and maximal likelihood factor analysis upon data generated from 280 undergraduate students, the factors of Concern about Health Knowledge, self-Concept, Information - Seeking Behavior, Decision -making and Responsibility, Self-esteem, and Passivity emerged. The total percent of variance explained by the 6 factors was 75.1% 2. To assess factor independence and instrument homogeneity, correlations among the 6 factors were computed. The correlations ranged from .24 to .46 indicating that the factors and the items were not too similar or redundant. 3. Test-retest reliability of the total scale is r=.70. Cronbach's $\alpha$ coefficient for internal consistency of the total scale is .86 and that of the factors ranged from .750 to .661 (only factor 6 .497). 4. In additional analysis of the Exercise of Self-Care Agency Scale in relationship to Multiple Health Locus of Control, Family Environmental Scale, and Cornell Medical Index using stepwise multiple regression, the Internal Health Locus of Control Score predicted 21.8%(F=53.34, p=.0001), Family Environmental Score 8.3%(F=22.59, P=.0001), Modified Cornell Medical Index Score 5.4%(F=15.74, P=.0001) of the score of the E.S.C.A.

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Acanthoparyphium shinanense n. sp. (Digenea: Echinostomatidae) from Experimental Chicks Infected with Metacercariae Encysted in Brackish Water Clams in the Republic of Korea

  • Ryoo, Seungwan;Jung, Bong-Kwang;Chang, Taehee;Hong, Sooji;Shin, Hyejoo;Chai, Jong-Yil
    • Parasites, Hosts and Diseases
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    • v.59 no.4
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    • pp.341-353
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    • 2021
  • Acanthoparyphium shinanense n. sp. (Digenea: Echinostomatidae) is described from chicks experimentally infected with the metacercariae encysted in 2 brackish water clam species, Ruditapes philippinarum and Coecella chinensis, in the Republic of Korea. The metacercariae were round to oval, armed with 23 collar spines, and 0.216 (0.203-0.226) mm in diameter. From 5 chicks experimentally infected each with 200 metacercariae, 34 juvenile (5-day-old worms) and 104 adult flukes (7-day-old worms) were harvested from their small intestines, with the average worm recovery rate of 13.8%. The adult flukes were 3.18 (2.89-3.55) mm long and 0.68 (0.61-0.85) mm wide, with an elongated, posteriorly tapering body, and a prominent head collar armed with 23 collar spines arranged in a single uninterrupted row. The posterior testis of A. shinanense was longitudinally elongated, which is similar to Acanthoparyphium spinulosum Johnston, 1917 but unique from the other closely related species, including Acanthoparyphium tyosenense Yamaguti, 1939, Acanthoparyphium kurogamo Yamaguti, 1939, and Acanthoparyphium marilae Yamaguti, 1934. The eggs of A. shinanense were larger than those of A. spinulosum, and the anterior extent of 2 lateral groups of vitellaria was slightly more limited in A. shinanense than in A. spinulosum. Molecular analysis of nuclear and mitochondrial genes revealed low homology with A. spinulosum from USA (96.1% in 5.8S rRNA) and Ukraine (97.9% in 28S rRNA), Acanthoparyphium n. sp. from USA (98.0% in 28S rRNA), and Acanthoparyphium sp. from Australia, Kuwait, and New Zealand. Biological characteristics, including its first intermediate host and natural definitive hosts, as well as its zoonotic capability, should be elucidated.

The Study on Attrition Resistance of ZnO/natural-zeolite/Fe$_2$O$_3$ Desulfurization Sorbents with CaO for Hot Gas Clean-up (산화칼슘이 첨가된 ZnO/Natural-zeolite/Balho Kim/Fe$_2$O$_3$ 탈황제의 내마모성특성 연구)

  • 정용길;박노국;이종대;전진혁;류시옥;이태진
    • Journal of Energy Engineering
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    • v.13 no.1
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    • pp.75-81
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    • 2004
  • ZZFCa sorbents for hot gas desulfurization in IGCC were prepared by adding calcium oxide to ZZF sorbent in order to improve its attrition resistance in this study. ASTM attrition test for the sorbent was performed at several different weight percentages of CaO to investigate the attrition characteristics of ZBFCa sorbents as a function of CaO content. Attrition index of ZZF without CaO was 28.3% and its collected attrition index was 10.8%. ZZFCa-3 containing 3 wt% CaO showed the lowest attrition index (AI=17.3%, CAI=8.8%) in the test. From the results of SEM morphologies and particle size distribution measurements, ZZFCa-3 maintained a fine shape and a desirable average particle size even after attrition test. In the experiments of sulfidation/regeneration for ZZFCa-3 sorbent concentration of hydrogen sulfide in coal gas was lowered from 10000 ppm to below 1 ppm. Sulfur removing capacity was about 28.8 g S/100 g sorbent. Neither formation of CaSO$_4$ was observed in XRD measurement nor SO$_2$ slippage was observed during sulfidation process.

Analysis of rDNA ITS Region from Trametes spp. in Kangwon Province, Korea (강원도 지역 구름버섯균의 rDNA의 ITS 부위 염기서열 분석)

  • Lee, Mi-Jeong;Jun, Sang-Cheol;Hwang, Il-Ki;Choi, Han-Ku;Kim, Kyu-Joong
    • The Korean Journal of Mycology
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    • v.33 no.1
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    • pp.1-10
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    • 2005
  • Nineteen strains of Trametes species were collected from the area of Kangwon Province, Korea. They have a variety of color hands and line-up markings on fruit bodies. Most strains were categorized into four types based on color bands, that is, dark brown, light brown, dark gray and light gray. They also have line-up marking shapes from sparse to compact on fruit bodies. In this study, we tried to investigate the relationship between the genetic variation and morphological appearance of these species using the nuclear ribosomal ITS1-5.8S-ITS2 region sequence, we used nineteen strains collected in nature and four species of five standard strains (T. versicolor KCTC16781, KCTC26203, T. villosa KCTC06866, T. suaveolens KCTC26205 and T. hirusta KCTC26200). The data of ITS sequences indicated that nineteen strains of T. versicolor have the difference of $1{\sim}6$ base pairs, comparing with standard strains of T. versicolor KCTC16781, and KCTC26203. Phylogenetic analysis of the Trametes species showed that they grouped into a wide range of single clade. Standard strains except T. versicolor KCTC16781 and KCTC26203, formed separated subgroup.

Occurrence and Molecular Identification of Anisakis Dujardin, 1845 from Marine Fish in Southern Makassar Strait, Indonesia

  • Anshary, Hilal;Sriwulan, Sriwulan;Freeman, Mark A.;Ogawa, Kazuo
    • Parasites, Hosts and Diseases
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    • v.52 no.1
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    • pp.9-19
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    • 2014
  • Anisakis spp. (Nematoda: Anisakidae) parasitize a wide range of marine animals, mammals serving as the definitive host and different fish species as intermediate or paratenic hosts. In this study, 18 fish species were investigated for Anisakis infection. Katsuwonus pelamis, Euthynnus affinis, Caranx sp., and Auxis thazard were infected with high prevalence of Anisakis type I, while Cephalopholis cyanostigma and Rastrelliger kanagurta revealed low prevalence. The mean intensity of Anisakis larvae in K. pelamis and A. thazard was 49.7 and 5.6, respectively. A total of 73 Anisakis type I larvae collected from K. pelamis and A. thazard were all identified as Anisakis typica by PCR-RFLP analysis. Five specimens of Anisakis from K. pelamis and 15 specimens from A. thazard were sequenced using ITS1-5.8S-ITS2 region and 6 specimens from A. thazard and 4 specimens from K. pelamis were sequenced in mtDNA cox2 region. Alignments of the samples in the ITS region showed 2 patterns of nucleotides. The first pattern (genotype) of Anisakis from A. thazard had 100% similarity with adult A. typica from dolphins from USA, whereas the second genotype from A. thazard and K. pelamis had 4 base pairs different in ITS1 region with adult A. typica from USA. In the mtDNA cox2 regions, Anisakis type I specimens from A. thazard and K. pelamis showed similarity range from 94% to 99% with A. typica AB517571/DQ116427. The difference of 4 bp nucleotides in ITS1 regions and divergence into 2 subgroups in mtDNA cox2 indicating the existence of A. typica sibling species in the Makassar Strait.