• Title/Summary/Keyword: ITS sequence

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Interspecific Distinguishability of Veiled Lady Mushrooms (Dictyophora spp.) Based on rDNA-ITS Analysis (rDNA-ITS 분석에 의한 망태버섯속균(Dictyophora spp.)의 종간 구분 가능성)

  • Cheong, Jong-Chun;Lee, Myung-Chul;Kim, Bum-Gi;Park, Dong-Seok;Hong, Sung-Beom;Park, Jeong-Sik
    • The Korean Journal of Mycology
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    • v.32 no.1
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    • pp.1-7
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    • 2004
  • To establish the phylogenetic relationships of Dictyophora spp., rDNA-ITS regions of 11 strains of veiled lady mushroom collected from various countries were amplified and sequenced. It was observed that the 11 strains were divided into four groups based on PCR band patterns of each ITS region cleaved by eight different restriction enzymes in cleaved amplified polymorphic sequence analysis (CAPS). The phylogenic relationship of each group by cleaved amplified polymorphic sequence (CAPS) analysis matches well with previously reported morphological phylogeny, such as 5 strains of D. indusiata, 4 strains of D. echinovolvata, and a strain of Phallus rugulosus. Sequence analysis using the cluster V methods showed more detail classification than CAPS analysis. The 5.8S region showed two point nucleotide base exchanges from G to A according to four groups, and four groups were subdivided by sequence variation of ITS I and ITS II regions. But sequence variation of Phallus rugulosus was not showed in full ITS region. This study further delineates the taxonomic level at which ITS sequences, in comparison to ribosomal gene sequence, are most useful in systematics and other mushroom study.

A Design Method of Discrete Time Learning Control System (이산시간 학습제어 시스템의 설계법)

  • 최순철
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.13 no.5
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    • pp.422-428
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    • 1988
  • An iterative learning control system is a control system which makes system outputs follow desired outputs by iterating its trials over a finite time interval. In a discrete time system, we proposed one method in which present control inputs can be obtained by a linear combination of the input sequence and time-shifted error sequence at previous trial. In contrast with a continous time learning control system which needs differential opreration of an error signal, the time shift operation of the error sequence is simpler in a computer control system and its effectiveness is shown by a simulation.

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A Novel Similarity Measure for Sequence Data

  • Pandi, Mohammad. H.;Kashefi, Omid;Minaei, Behrouz
    • Journal of Information Processing Systems
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    • v.7 no.3
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    • pp.413-424
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    • 2011
  • A variety of different metrics has been introduced to measure the similarity of two given sequences. These widely used metrics are ranging from spell correctors and categorizers to new sequence mining applications. Different metrics consider different aspects of sequences, but the essence of any sequence is extracted from the ordering of its elements. In this paper, we propose a novel sequence similarity measure that is based on all ordered pairs of one sequence and where a Hasse diagram is built in the other sequence. In contrast with existing approaches, the idea behind the proposed sequence similarity metric is to extract all ordering features to capture sequence properties. We designed a clustering problem to evaluate our sequence similarity metric. Experimental results showed the superiority of our proposed sequence similarity metric in maximizing the purity of clustering compared to metrics such as d2, Smith-Waterman, Levenshtein, and Needleman-Wunsch. The limitation of those methods originates from some neglected sequence features, which are considered in our proposed sequence similarity metric.

Bioinformatics in Fish: its Present Status and Perspectives with Particular Emphasis on Expressed Sequence Tags

  • Nam, Yoon-Kwon;Kim, Dong-Soo
    • Journal of Aquaculture
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    • v.14 no.1
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    • pp.9-16
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    • 2001
  • Characterization of a single pass of cDNA sequence, an expressed sequence tag (EST) has been a fast growing activity in fish genomics. Despite its relatively short history, fish EST databases (dbESTs) have already begun to play a significant role in bridging the gaps in our knowledge on the gene expression in fish genome. This review provides a brief description of the technology for establishing fish dbESTs, its current status, and implication of the ESTs to aquaculture and fisheries science with particular emphasis on the discovery of novel genes for transgenic application, the use of polymorphic EST markers in genetic linkage mapping and the evaluation of signal-responsive gene expression.

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Molecular Phylogenetic Classification of Dermatophytes Isolated from Dogs and Cats (개와 고양이 유래 피부사상균의 분자생물학적 계통 분석)

  • Kim, Doo;Jeoung, Seok-Young;Ahn, So-Jeo
    • Journal of Veterinary Clinics
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    • v.23 no.4
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    • pp.405-410
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    • 2006
  • Using internal transcribed spacer 1 (ITS1) region ribosomal DNA sequences from 9 strains of Microsporum canis and 5 strains of Microsporum gypseum isolated from dogs and a cat with dermatophytosis, we demonstrated the mutual phylogenetic relationship of these strains. Nucleotide sequence analysis of the ITS 1 gene fragments from the 9 strains of M canis had the 100% nucleotide sequence similarities and the 5 strains of M gypseum also had the 100% nucleotide sequence similarities. The phylogenetic analysis of the nucleotide sequences of the 9 strains of M canis formed a nested cluster with the reference strains of M canis originating from USA, Australia, Japan, and Europe. M canis were genetically distinct from the other reference strains of Microsporum spp, but M distortum, M equinum, and M. ferrugineum were genetically very close to M canis. M gypseum from a cluster in the phylogenetic tree with M canis as an outgroup. The molecular analysis of ITS 1 genes provided the useful information for the identification of these microsporum species and the understanding of their relationship.

Genetic Relationships of Four Korean Oysters Based on RAPD and Nuclear rDNA ITS Sequence Analyses

  • Kim, Woo-Jin;Lee, Jeong-Ho;Kim, Kyung-Kil;Kim, Young-Ok;Nam, Bo-Hye;Kong, Hee-Jeong;Jung, Hyung-Taek
    • The Korean Journal of Malacology
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    • v.25 no.1
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    • pp.41-49
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    • 2009
  • Random amplified polymorphic DNA (RAPD) marker and sequence analyses of the internal transcribed spacer (ITS) region of ribosomal DNA were used to assess phylogenetic relationships of four Korean oyster species. The average number of species-specific markers identified from five universal rice primers (URPs) by RAPD-PCR was 1.8 for Crassostrea gigas, 3.2 for C. nippona, 3.6 for C. ariakensis, and 4.6 for Ostrea denselamellosa. The length of the ITS (ITS1-5.8S-ITS2) region ranged from 1,001 to 1,206 bp (ITS1, 426-518 bp; 5.8S, 157 bp; and ITS2, 418-536 bp), while the GC content ranged from 55.5-61.1% (ITS1, 56.8-61.8%; 5.8S, 56-57.3%; and ITS2, 54.1-62.2%). A phylogenetic analysis of the oysters based on our RAPD, ITS1, and ITS2 sequence data revealed a close relationship between C. gigas and C. nippona and a distant relationship between the genera Crassostrea and Ostrea. Our results indicated that RAPD and ITS sequence analysis was a useful tool for the elucidation of phylogenetic relationships and for the selection of species-specific markers in Korean oysters.

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Molecular Identification of Two Strains of Phellinus sp. by Internal Transcribed Spacer Sequence Analysis

  • Shin, Kwang-Soo
    • Mycobiology
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    • v.39 no.4
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    • pp.299-300
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    • 2011
  • Two species of cultivated Phellinus sp. were identified as P. baumii by internal transcribed spacer (ITS) sequence analysis. The fruit bodies of the examined strains were similar to those of naturally occurring strains, having a bracket-like form, yellow-to-orange color, and poroid hymenial surfaces. The DNA sequences of ITS region of both strains showed a homology of 99% with ITS1 to ITS2 sequences of P. (Inonotus) baumii strain PB0806.

Genetic Variation of Rice Populations Estimated Using nrDNA ITS Region Sequence

  • Wang, Dong;Hong, Soon-Kwan
    • Korean Journal of Plant Resources
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    • v.27 no.3
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    • pp.249-255
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    • 2014
  • The rice belonging to Oryza sativa is not only has significant economic importance, for it is the major source of nutrition for about 3 billion all around the world. But also plays a vital role as a model organism, because it has a number of advantages to be a model plant, such as efficient transformation system and small genome size. Many methods and techniques have been conducted to attempt to distinguish different Oryza sativa species, such as amplified fragment length polymorphism (AFLP), random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR) and so on. However, studies using sequence analysis of internal transcribed spacer (ITS), a region of ribosomal RNA has not been reported until now. This study was undertaken with an aim to understand the phylogenetic relationships among sixteen isolates of Oryza sativa collected from abroad and fifteen isolates collected from Korea, using ribosomal RNA (rRNA) internal transcribed spacer (ITS) sequences to compare the phylogeny relationships among different Oryza sativa species. The size variation obtained among sequenced nuclear ribosomal DNA (nrDNA) ITS region ranged from 515bp to 1000bp. The highest interspecific genetic distance (GD) was found between Sfejare 45 (FR12) and Anapuruna (FR15). Taebong isolate showed the least dissimilarity of the ITS region sequence with other thirty isolates. This consequence will help us further understanding molecular diversification in intra-species population and their phylogenetic analysis.

THE NORM RATIO OF THE POLYNOMIALS WITH COEFFICIENTS AS BINARY SEQUENCE

  • Taghavi, M.
    • Journal of applied mathematics & informatics
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    • v.13 no.1_2
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    • pp.195-200
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    • 2003
  • Given a positive integer q, the ratio of the 2q-norm of a polynomial which its coefficients form a binary sequence and its 2-norm arose from telecommunication engineering consists of finding any type of such polynomials haying the ratio “small” In this paper we consider some special types of these polynomials, discuss the sharpest possible upper bound, and prove a result for the ratio.

STATISTICALLY LOCALIZED SEQUENCES IN 2-NORMED SPACES

  • Yamanci, Ulas;Nabiev, Anar Adiloglu;Gurdal, Mehmet
    • Honam Mathematical Journal
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    • v.42 no.1
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    • pp.161-173
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    • 2020
  • We introduce statistically localized sequences in 2-normed spaces and give some main properties of statistically localized sequences. Also, we prove that a sequence is statistically Cauchy sequence if and only if its statistical barrier is equal to zero. Moreover, we define the uniformly statistically localized sequences on 2-normed spaces and investigate its relationship with statistically Cauchy sequences.